MD05G1196500.v1.1

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006326
Physical Location & Seq
Forward (+)
32380698 .. 32389677
8980 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1196500.v1.1.491

Sequence Viewer

Length: 3660 bp
ATGCCGTCCCTGGTCTCTCAGGGCAAAATCCTCCGCCTGGAGCTCGAGAACTTCAAGTCCTACAAGGGCTTCCAGACCATCGGCCCCTTCTACGACTTCACTGCGATTATTGGGCCCAATGGCGCCGGAAAGTCCAACCTCATGGACGCCATAAGCTTCGTTCTCGGCGTGCGGACGGGCCACCTCCGTGGGGCCCAGCTCAAGGACCTGATCTATGCCTTCGACGACAGGGAGAAGGACCAGAAGGGCCGCAGGGCCTTTGTCCGCTTGGTTTACCAGCTGGCCAGCGGGTCGGAGCTCCAGTTCACCCGAGCCATTACCGGCGGAGCGGGCAGCGAGTACCGTATCGATGGGGCGTCTGTGACGTGGGAAGAGTACAATGCGAAGCTCAGGTCTCTTGGGATTCTTGTCAAGGCCCGGAATTTCCTCGTCTTTCAGGGCGATGTGGAGTCTATTGCCTCCAAAAATCCCAAGGAACTTACTGCACTTCTTGAGCAGATCTCTGGGTCTGATGAACTCAAGAGAGACTATGAAAAATATGAAGAAGAAAAATCCGAAGCTGAACAAAAAGCAAGTCTTGTTTATCAGAGAAAGAGAACAATAGTATTGGAGAGGAGGCAAAAGAAAGAACAGAAAGAAGAAGCTGAGAAACACCTCCGTCTGCAAGATCAACTGAAATCTTTGAAGAAAGAACATTCTTTATGGCAATTGTTCAACATAGAAAAGGATATTGCAAAAATGACTGAGGAGCTTGAAGCTGAAAAGAAAAGCCGCGAACAAGTTATGCAAGAAATTGATGAGTTTCAGCTAGAAGCAAACAATAAGAAGAAAGAACAAGCAAAATATCTGAAAGAAATTGCACAATGTGAAAAGAAGATTTCAGATAGAAATAGTAAACTTGACAAAAGTCAACCAGACCTTCTGAAACTGAAAGAGGAAATGTCTCGTATAAATGCAAAAATCAAGAAAAGTGAGAAGGAGCTTGCTAAGAAAGAACAAGAAAGGAAAAGACATAAAGAGGATATAAAACAGCTGCAGAAGGGAATACAGGATCTGACTGCAAAACTAGAAGATTTACATGAACAAGGCCGAGATAGTGGCGACAAACTGAAGTTAGACGATACTGAATTAAGGGAATACTTTCGAATCAAGGAAGATGCTGGGATGAAAACTGGAAGGCTAAGAGATGAGAAAGAGGTCCTGGATAGGCAACAACATGCTGATCTTGAAGCTCAAAAGAATTTGGAAGAAAATCTTCAACAGTTGAGAAGTCGGGAGGCTGAATTAGAGGAACAAGAGGAACAAATGCAAACAAGGCAGAAAAAAATTAAAGAAAATTCTGCGAGACATAGGGATGAAGCTAAAAATCTGGATAATGAACTGCATGCCATGCAATCTAAAAATTCACAGGCCAGAACGAAATATGAAAATCTGAAGTCAAAAATTGATGAAATAGATAAGCAACTACGGGAATTGAAGGCTGACAGATATGAGAATGAGAGGGATTCCAGGTTGTCTCAGGCAGTCGAGACTCTCAAACGCCTGTTTCACGGTGTCCACGGTCGTATGACTGAACTCTGTAGGCCAACACAGAAAAAGTATAACCTCGCTGTTACTGTTGCCATGGGTAAATTTATGGATGCAGTTGTAGTTGAGGACGAACAAACGGGAAAGGAATGCATCAAGTATTTGAAAGAACAAAGGCTTCCCCCTCAGACATTCATACCTCTTCAGTCTGTCCGTGTGAAGCCCGTAATGGAGAGATTGCGCAACTTAGGTGGTACTGCAAAGCTGATCTTTGATGTAGCAATTCTTTTTGCGGTTGGCAATACTCTTGTCTGTGATGAACTTGATGAGGCTAAGCGTCTCAGCTGGACTGGTGAGAGGTTCAAAGTTGTAACTGTTGATGGAATTCTGCTTGCCAAATCTGGCACAATGACTGGCGGTACAAGTGGTGGAATGGAAGCAAGGTCAAAACAGTGGGACGATAAAAAAGTTGAAGGACTAAAGAAGAAGAAAGAACAATTTGAGTCAGAGTTGGAAGAGCTAGGGTCAATTAGAGAGATGCAGATAAAAGAGTCTGAGACAACTGGTAGAATTAGTGGACTCCAAAAAAAAATCCAGTATGCAGAGATTGAGAAGAAAAGCATCAAGGACAAACTTGCAACCTTGGCACGGGAAAAGCAGAATATAAAAGAAGAGATTGATCGCAGCAGTCCTGAACTTCTTAAGTTAAAGCAAGCTGTAGATAAGCGGAGCAAGGAAATCAATAAGCTTGAGAAGAGGATAAATGAAATTGTTGACAGAATCTACAAAGATTTTAGTAAATCTGTTGGGGTGGCAAACATCCGTGAGTATGAGGAAAATCAACTCAAGGCTTCCCAATATATGGCTGAAGAGAGGCTTAGTTTGAGTAGCCAGCTGTCAAAGTTGAAATACCAGTTAGAGTATGAGCAAAACCGGGACATGGAATCACGAATTAACGAACTCCAAGATTCTATTAGTAAATTTAAAAAAGCTTTAGAACGGGTTCAGAAGGAAGAGGCTGCAGCCAAGTCAGCAGCAGAAAAAGCTTCTGGTGAGATTGATCAGTGGAAGAAAGAAATACAAGAATGGAAATCCAAGTCAGAAGGGTGTGAGAAGGAAATCCAAGAATGGAACAAGCGGGGTTCTACAGCTACAACAAGTTTATCCAAACTTAATCGACAGATACACGCTAAGGAGTCACAGATTGAGCAGCTGATGTCACAGAAGCAGGAAATAGTAGAGAAATGTGAATTAGAACAAATAAACCTTCCCATCATCTCAGACCCAATGGAGATTGATTCCTCCCCAATGGGCCCAGTCTTCGATTTTAGTCAGTTAAACAAGTCTCATATGCGGGACAGAAGGCCCTCTGAACGGGAGAAGCTTGAGGTGGAATTTAAGCAAAAAATGGATGCCGTAATTTCAGAAATTGAAAAAACAGCTCCAAATTTGAAGGCCCTGGACCAATATGAGGCAATAAAAGAAAAGGAAAGAGCTGTAACTGAAGAGTTTGAAGTGGCTAGAATAGAAGAGAAGGAAAAAGCTGATATGTTTAACTCAGTTAAGCAGAAGAGGTACGAGTTGTTTATGGATGCCTTCCAACATATTTCCAGTAACATTGATAAGATATACAAACAACTGACAAAGAGCAACACACATCCACTGGGTGGGACGGCATATTTAAATTTGGAAAATGAAGATGATCCATTTTTACATGGCATTAAGTACACTGCTATGCCCCCAACAAAGCGCTTTCGTGATATGGAACAACTATCTGGCGGCGAGAAAACTGTTGCAGCATTGGCATTACTCTTTTCCATACACAGGCTTCTATTTCCTCTTATGCATGGGTTCAGTTTTAGGCCTTCACCGTTTTTCATACTGGATGAAGTCGATGCTGCACTAGATAACTTGAATGTTGCTAAGGTTGCTGGATTCATCCGTTCAAAGGCACGTGAAGGAGCCAGGGCTAACCAAGATGATGAGGGAGGCAATGGTTTTCAGAGCATTGTAATATCTCTAAAAGATAGCTTCTACGACAAGGCTGATGCTTTAGTTGGGGTTTACAGGGACTGCGAAAGGAGCTGTTCGGAAACTCTGACATTTGACCTGACCAAGTATCGGGAGTCGTGA

Protein Analysis

1220

Amino Acids

140.8

Weight (kDa)

7.98

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_15 PF13175 9 - 347 2.4e-13 AAA ATPase domain
SMC_N PF02463 10 - 1199 5.5e-36 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 12 - 231 8.9e-15 AAA domain
AAA_29 PF13555 12 - 55 7.6e-07 P-loop containing region of AAA domain
AAA_21 PF13304 33 - 62 4.4e-07 AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge PF06470 516 - 623 2.1e-20 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1769
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 2 cut(s) 2389, 3194
AccBSI CCGCTC 1 cut(s) 329
AccII CGCG 1 cut(s) 774
AclWI GGATC 2 cut(s) 1059, 3224
AcoI YGGCCR 1 cut(s) 282
AcuI CTGAAG 5 cut(s) 1130, 1454, 1715, 2415, 3051
AcvI CACGTG 1 cut(s) 3482
AcyI GRCGYC 3 cut(s) 123, 147, 356
AdeI CACNNNGTG 2 cut(s) 866, 3194
AfaI GTAC 6 cut(s) 341, 377, 1783, 1948, 3104, 3254
AfeI AGCGCT 1 cut(s) 3278
AflII CTTAAG 1 cut(s) 2228
AjiI CACGTC 1 cut(s) 366
AjnI CCWGG 6 cut(s) 9, 36, 1200, 1508, 2985, 3491
AleI CACNNNNGTG 1 cut(s) 186
AloI GAACNNNNNNTCC 4 cut(s) 41, 73, 287, 319
Alw21I GWGCWC 2 cut(s) 45, 300
AlwI GGATC 2 cut(s) 1059, 3224
AlwNI CAGNNNCTG 2 cut(s) 1054, 3600
Ama87I CYCGRG 2 cut(s) 44, 309
Aor51HI AGCGCT 1 cut(s) 3278
ApaI GGGCCC 3 cut(s) 117, 196, 2843
ApeKI GCWGC 9 cut(s) 333, 1033, 2211, 2546, 2549, 2561, 2737, 3323, 3425
ArsI GACNNNNNNTTYG 4 cut(s) 377, 409, 559, 591
Asp700I GAANNNNTTC 3 cut(s) 1140, 1254, 2529
AspLEI GCGC 3 cut(s) 125, 1770, 3279
AsuC2I CCSGG 2 cut(s) 418, 2462
AsuHPI GGTGA 4 cut(s) 298, 1892, 2591, 3387
AsuII TTCGAA 1 cut(s) 1144
AvaI CYCGRG 2 cut(s) 44, 309
AvaII GGWCC 4 cut(s) 205, 238, 1198, 2989
BaeGI GKGCMC 3 cut(s) 117, 196, 2843
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 5 cut(s) 45, 117, 196, 300, 2843
BbrPI CACGTG 1 cut(s) 3482
BbsI GAAGAC 1 cut(s) 2839
Bbv12I GWGCWC 2 cut(s) 45, 300
BbvI GCAGC 9 cut(s) 345, 1020, 2223, 2533, 2561, 2573, 2749, 3335, 3412
BccI CCATC 4 cut(s) 86, 344, 1901, 2807
BceAI ACGGC 2 cut(s) 2927, 3216
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BciT130I CCWGG 6 cut(s) 11, 38, 1202, 1510, 2987, 3493
BclI TGATCA 1 cut(s) 2587
BcnI CCSGG 2 cut(s) 418, 2462
BfaI CTAG 5 cut(s) 809, 1067, 2048, 3048, 3431
BfmI CTRYAG 5 cut(s) 1034, 1579, 2244, 2547, 2673
BfoI RGCGCY 2 cut(s) 126, 3280
BfrI CTTAAG 1 cut(s) 2228
BglII AGATCT 1 cut(s) 498
BlpI GCTNAGC 1 cut(s) 1860
Bme1390I CCNGG 8 cut(s) 11, 38, 418, 1202, 1510, 2462, 2987, 3493
Bme18I GGWCC 4 cut(s) 205, 238, 1198, 2989
BmeT110I CYCGRG 2 cut(s) 44, 309
BmgBI CACGTC 1 cut(s) 366
BmiI GGNNCC 7 cut(s) 85, 115, 124, 193, 194, 2841, 3490
BmrFI CCNGG 8 cut(s) 11, 38, 418, 1202, 1510, 2462, 2987, 3493
BmrI ACTGGG 2 cut(s) 2837, 3200
BmsI GCATC 9 cut(s) 1147, 1630, 1689, 2055, 2157, 2929, 3109, 3412, 3565
BmuI ACTGGG 2 cut(s) 2837, 3200
BoxI GACNNNNGTC 1 cut(s) 906
BpiI GAAGAC 1 cut(s) 2839
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 2 cut(s) 59, 284
Bpu10I CCTNAGC 3 cut(s) 389, 2718, 3450
Bpu1102I GCTNAGC 1 cut(s) 1860
Bpu14I TTCGAA 1 cut(s) 1144
BpuEI CTTGAG 6 cut(s) 185, 503, 512, 2297, 2357, 2933
BpuMI CCSGG 2 cut(s) 418, 2462
Bsa29I ATCGAT 1 cut(s) 348
BsaAI YACGTR 1 cut(s) 3482
BsaHI GRCGYC 3 cut(s) 123, 147, 356
BsaI GGTCTC 2 cut(s) 19, 399
BsaJI CCNNGG 8 cut(s) 9, 187, 471, 1558, 1623, 2169, 2985, 3492
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bse118I RCCGGY 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 3526
BseBI CCWGG 6 cut(s) 11, 38, 1202, 1510, 2987, 3493
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 8 cut(s) 9, 187, 471, 1558, 1623, 2169, 2985, 3492
BseGI GGATG 9 cut(s) 1170, 1360, 1645, 2346, 2944, 3124, 3184, 3418, 3465
BseMI GCAATG 1 cut(s) 3526
BseRI GAGGAG 2 cut(s) 628, 761
BseSI GKGCMC 3 cut(s) 117, 196, 2843
BseXI GCAGC 9 cut(s) 345, 1020, 2223, 2533, 2561, 2573, 2749, 3335, 3412
BseYI CCCAGC 2 cut(s) 195, 1160
BsgI GTGCAG 2 cut(s) 468, 3411
Bsh1236I CGCG 1 cut(s) 774
Bsh1285I CGRYCG 1 cut(s) 1564
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 348
BsiEI CGRYCG 1 cut(s) 1564
BsiHKAI GWGCWC 2 cut(s) 45, 300
BsiHKCI CYCGRG 2 cut(s) 44, 309
BsiSI CCGG 4 cut(s) 126, 321, 418, 2461
BslFI GGGAC 5 cut(s) 1997, 2477, 2897, 3211, 3611
BsmBI CGTCTC 1 cut(s) 1871
BsmFI GGGAC 5 cut(s) 1997, 2477, 2897, 3211, 3611
BsmI GAATGC 1 cut(s) 1682
Bso31I GGTCTC 2 cut(s) 19, 399
BsoBI CYCGRG 2 cut(s) 44, 309
Bsp119I TTCGAA 1 cut(s) 1144
Bsp120I GGGCCC 3 cut(s) 113, 192, 2839
Bsp1286I GDGCHC 5 cut(s) 45, 117, 196, 300, 2843
Bsp143I GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
Bsp1720I GCTNAGC 1 cut(s) 1860
Bsp19I CCATGG 1 cut(s) 1623
BspDI ATCGAT 1 cut(s) 348
BspFNI CGCG 1 cut(s) 774
BspLI GGNNCC 7 cut(s) 85, 115, 124, 193, 194, 2841, 3490
BspMAI CTGCAG 2 cut(s) 1038, 2551
BspPI GGATC 2 cut(s) 1059, 3224
BspQI GCTCTTC 1 cut(s) 2037
BspT104I TTCGAA 1 cut(s) 1144
BspT107I GGYRCC 1 cut(s) 122
BspTI CTTAAG 1 cut(s) 2228
BspTNI GGTCTC 2 cut(s) 19, 399
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 3526
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 8 cut(s) 9, 187, 471, 1558, 1623, 2169, 2985, 3492
BssMI GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
BssNI GRCGYC 3 cut(s) 123, 147, 356
BssT1I CCWWGG 3 cut(s) 471, 1623, 2169
Bst2UI CCWGG 6 cut(s) 11, 38, 1202, 1510, 2987, 3493
Bst4CI ACNGT 9 cut(s) 344, 1263, 1553, 1562, 1618, 1903, 1980, 3319, 3399
BstACI GRCGYC 3 cut(s) 123, 147, 356
BstAFI CTTAAG 1 cut(s) 2228
BstAPI GCANNNNNTGC 1 cut(s) 1390
BstBAI YACGTR 1 cut(s) 3482
BstBI TTCGAA 1 cut(s) 1144
BstC8I GCNNGC 9 cut(s) 170, 282, 286, 331, 984, 1386, 1920, 2241, 2420
BstDSI CCRYGG 3 cut(s) 187, 1558, 1623
BstF5I GGATG 9 cut(s) 1170, 1360, 1645, 2346, 2944, 3124, 3184, 3418, 3465
BstFNI CGCG 1 cut(s) 774
BstH2I RGCGCY 2 cut(s) 126, 3280
BstHHI GCGC 3 cut(s) 125, 1770, 3279
BstKTI GATC 9 cut(s) 213, 501, 670, 1054, 1225, 1797, 2209, 2590, 3232
BstMBI GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
BstMCI CGRYCG 1 cut(s) 1564
BstMWI GCNNNNNNNGC 9 cut(s) 330, 1315, 1390, 2171, 2558, 2570, 3329, 3455, 3609
BstNI CCWGG 6 cut(s) 11, 38, 1202, 1510, 2987, 3493
BstNSI RCATGY 2 cut(s) 1220, 1388
BstPAI GACNNNNGTC 1 cut(s) 906
BstSCI CCNGG 8 cut(s) 9, 36, 416, 1200, 1508, 2460, 2985, 3491
BstSFI CTRYAG 5 cut(s) 1034, 1579, 2244, 2547, 2673
BstSLI GKGCMC 3 cut(s) 117, 196, 2843
BstUI CGCG 1 cut(s) 774
BstV1I GCAGC 9 cut(s) 345, 1020, 2223, 2533, 2561, 2573, 2749, 3335, 3412
BstV2I GAAGAC 1 cut(s) 2839
BstX2I RGATCY 2 cut(s) 498, 1051
BstXI CCANNNNNNTGG 2 cut(s) 142, 188
BstYI RGATCY 2 cut(s) 498, 1051
Bsu15I ATCGAT 1 cut(s) 348
BsuTUI ATCGAT 1 cut(s) 348
BtgI CCRYGG 3 cut(s) 187, 1558, 1623
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 366
BtsCI GGATG 9 cut(s) 1170, 1360, 1645, 2346, 2944, 3124, 3184, 3418, 3465
BtsI GCAGTG 2 cut(s) 99, 3255
BtsIMutI CAGTG 5 cut(s) 99, 1985, 2597, 3188, 3255
Cac8I GCNNGC 9 cut(s) 170, 282, 286, 331, 984, 1386, 1920, 2241, 2420
CaiI CAGNNNCTG 2 cut(s) 1054, 3600
CfoI GCGC 3 cut(s) 125, 1770, 3279
Cfr10I RCCGGY 1 cut(s) 320
ClaI ATCGAT 1 cut(s) 348
CseI GACGC 3 cut(s) 155, 345, 1853
Csp6I GTAC 6 cut(s) 340, 376, 1782, 1947, 3103, 3253
CspCI CAANNNNNGTGG 2 cut(s) 1759, 1794
CviAII CATG 9 cut(s) 142, 1079, 1217, 1385, 1390, 1624, 2467, 3242, 3374
CviQI GTAC 6 cut(s) 340, 376, 1782, 1947, 3103, 3253
DinI GGCGCC 1 cut(s) 124
DpnI GATC 9 cut(s) 212, 500, 669, 1053, 1224, 1796, 2208, 2589, 3231
DpnII GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
DraI TTTAAA 2 cut(s) 2512, 3210
DraIII CACNNNGTG 2 cut(s) 866, 3194
EaeI YGGCCR 1 cut(s) 282
EciI GGCGGA 2 cut(s) 23, 339
Ecl136II GAGCTC 2 cut(s) 43, 298
Eco130I CCWWGG 3 cut(s) 471, 1623, 2169
Eco147I AGGCCT 1 cut(s) 3391
Eco24I GRGCYC 5 cut(s) 45, 117, 196, 300, 2843
Eco31I GGTCTC 2 cut(s) 19, 399
Eco47I GGWCC 4 cut(s) 205, 238, 1198, 2989
Eco47III AGCGCT 1 cut(s) 3278
Eco53kI GAGCTC 2 cut(s) 43, 298
Eco57I CTGAAG 5 cut(s) 1130, 1454, 1715, 2415, 3051
Eco72I CACGTG 1 cut(s) 3482
Eco88I CYCGRG 2 cut(s) 44, 309
EcoICRI GAGCTC 2 cut(s) 43, 298
EcoO109I RGGNCCY 6 cut(s) 192, 205, 255, 1198, 2892, 2983
EcoRI GAATTC 1 cut(s) 1911
EcoRII CCWGG 6 cut(s) 9, 36, 1200, 1508, 2985, 3491
EcoT14I CCWWGG 3 cut(s) 471, 1623, 2169
EcoT22I ATGCAT 2 cut(s) 1682, 3375
EcoT38I GRGCYC 5 cut(s) 45, 117, 196, 300, 2843
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 3 cut(s) 471, 1623, 2169
Esp3I CGTCTC 1 cut(s) 1871
FaeI CATG 9 cut(s) 145, 1082, 1220, 1388, 1393, 1627, 2470, 3245, 3377
FalI AAGNNNNNCTT 8 cut(s) 561, 593, 1239, 1271, 1781, 1813, 2388, 2420
FaqI GGGAC 5 cut(s) 1997, 2477, 2897, 3211, 3611
FatI CATG 9 cut(s) 141, 1078, 1216, 1384, 1389, 1623, 2466, 3241, 3373
FauI CCCGC 4 cut(s) 281, 322, 2658, 2874
FauNDI CATATG 1 cut(s) 2877
FbaI TGATCA 1 cut(s) 2587
FokI GGATG 9 cut(s) 1177, 1367, 1652, 2333, 2951, 3131, 3171, 3425, 3452
FriOI GRGCYC 5 cut(s) 45, 117, 196, 300, 2843
FspBI CTAG 5 cut(s) 809, 1067, 2048, 3048, 3431
FspI TGCGCA 1 cut(s) 1769
GlaI GCGC 3 cut(s) 124, 1769, 3278
GsaI CCCAGC 2 cut(s) 199, 1164
GsuI CTGGAG 2 cut(s) 59, 284
HaeII RGCGCY 2 cut(s) 126, 3280
HapII CCGG 4 cut(s) 126, 321, 418, 2461
HgaI GACGC 3 cut(s) 155, 345, 1853
HhaI GCGC 3 cut(s) 125, 1770, 3279
Hin1I GRCGYC 3 cut(s) 123, 147, 356
Hin1II CATG 9 cut(s) 145, 1082, 1220, 1388, 1393, 1627, 2470, 3245, 3377
Hin6I GCGC 3 cut(s) 123, 1768, 3277
HinP1I GCGC 3 cut(s) 123, 1768, 3277
HincII GTYRAC 2 cut(s) 911, 2302
HindII GTYRAC 2 cut(s) 911, 2302
HindIII AAGCTT 5 cut(s) 154, 2273, 2517, 2571, 2909
HpaII CCGG 4 cut(s) 126, 321, 418, 2461
HphI GGTGA 4 cut(s) 298, 1892, 2591, 3387
Hpy166II GTNNAC 9 cut(s) 274, 306, 896, 911, 1558, 2105, 2302, 3255, 3592
Hpy8I GTNNAC 9 cut(s) 274, 306, 896, 911, 1558, 2105, 2302, 3255, 3592
Hpy99I CGWCG 1 cut(s) 227
HpyCH4III ACNGT 9 cut(s) 344, 1263, 1553, 1562, 1618, 1903, 1980, 3319, 3399
HpyCH4IV ACGT 2 cut(s) 365, 3481
HpyF10VI GCNNNNNNNGC 9 cut(s) 330, 1315, 1390, 2171, 2558, 2570, 3329, 3455, 3609
HpySE526I ACGT 2 cut(s) 365, 3481
Hsp92I GRCGYC 3 cut(s) 123, 147, 356
Hsp92II CATG 9 cut(s) 145, 1082, 1220, 1388, 1393, 1627, 2470, 3245, 3377
HspAI GCGC 3 cut(s) 123, 1768, 3277
KasI GGCGCC 1 cut(s) 122
Ksp22I TGATCA 1 cut(s) 2587
Kzo9I GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
LguI GCTCTTC 1 cut(s) 2037
Lsp1109I GCAGC 9 cut(s) 345, 1020, 2223, 2533, 2561, 2573, 2749, 3335, 3412
LweI GCATC 9 cut(s) 1147, 1630, 1689, 2055, 2157, 2929, 3109, 3412, 3565
MaeI CTAG 5 cut(s) 809, 1067, 2048, 3048, 3431
MaeII ACGT 2 cut(s) 365, 3481
MaeIII GTNAC 7 cut(s) 361, 1612, 1897, 2724, 2745, 3025, 3140
MalI GATC 9 cut(s) 212, 500, 669, 1053, 1224, 1796, 2208, 2589, 3231
MbiI CCGCTC 1 cut(s) 329
MboI GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
MfeI CAATTG 1 cut(s) 707
MflI RGATCY 2 cut(s) 498, 1051
MhlI GDGCHC 5 cut(s) 45, 117, 196, 300, 2843
MlsI TGGCCA 1 cut(s) 284
MluNI TGGCCA 1 cut(s) 284
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 6 cut(s) 458, 1525, 2039, 2087, 2100, 2732
MmeI TCCRAC 4 cut(s) 159, 273, 2019, 3151
Mox20I TGGCCA 1 cut(s) 284
Mph1103I ATGCAT 2 cut(s) 1682, 3375
MroXI GAANNNNTTC 3 cut(s) 1140, 1254, 2529
MscI TGGCCA 1 cut(s) 284
MslI CAYNNNNRTG 3 cut(s) 186, 1353, 3260
Msp20I TGGCCA 1 cut(s) 284
MspA1I CMGCKG 6 cut(s) 280, 288, 1033, 1872, 2422, 2740
MspCI CTTAAG 1 cut(s) 2228
MspI CCGG 4 cut(s) 126, 321, 418, 2461
MspR9I CCNGG 8 cut(s) 11, 38, 418, 1202, 1510, 2462, 2987, 3493
MunI CAATTG 1 cut(s) 707
Mva1269I GAATGC 1 cut(s) 1682
MvaI CCWGG 6 cut(s) 11, 38, 1202, 1510, 2987, 3493
MvnI CGCG 1 cut(s) 774
MwoI GCNNNNNNNGC 9 cut(s) 330, 1315, 1390, 2171, 2558, 2570, 3329, 3455, 3609
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 2 cut(s) 418, 2462
NcoI CCATGG 1 cut(s) 1623
NdeI CATATG 1 cut(s) 2877
NdeII GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
NlaIII CATG 9 cut(s) 145, 1082, 1220, 1388, 1393, 1627, 2470, 3245, 3377
NlaIV GGNNCC 7 cut(s) 85, 115, 124, 193, 194, 2841, 3490
NmeAIII GCCGAG 2 cut(s) 144, 1115
NmuCI GTSAC 3 cut(s) 361, 2724, 2745
NsbI TGCGCA 1 cut(s) 1769
NsiI ATGCAT 2 cut(s) 1682, 3375
NspI RCATGY 2 cut(s) 1220, 1388
NspV TTCGAA 1 cut(s) 1144
OliI CACNNNNGTG 1 cut(s) 186
PaeI GCATGC 1 cut(s) 1388
PaeR7I CTCGAG 1 cut(s) 44
PceI AGGCCT 1 cut(s) 3391
PciSI GCTCTTC 1 cut(s) 2037
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 1682
PdmI GAANNNNTTC 3 cut(s) 1140, 1254, 2529
PfeI GAWTC 8 cut(s) 403, 1146, 1505, 2307, 2471, 2495, 2825, 3462
PflMI CCANNNNNTGG 2 cut(s) 2389, 3194
PfoI TCCNGGA 1 cut(s) 1200
PleI GAGTC 6 cut(s) 457, 1525, 2038, 2086, 2100, 2731
PluTI GGCGCC 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 3482
PmlI CACGTG 1 cut(s) 3482
PpsI GAGTC 6 cut(s) 457, 1525, 2038, 2086, 2100, 2731
Ppu21I YACGTR 1 cut(s) 3482
PpuMI RGGWCCY 2 cut(s) 205, 1198
PshAI GACNNNNGTC 1 cut(s) 906
Psp124BI GAGCTC 2 cut(s) 45, 300
Psp5II RGGWCCY 2 cut(s) 205, 1198
Psp6I CCWGG 6 cut(s) 9, 36, 1200, 1508, 2985, 3491
PspCI CACGTG 1 cut(s) 3482
PspFI CCCAGC 2 cut(s) 195, 1160
PspGI CCWGG 6 cut(s) 9, 36, 1200, 1508, 2985, 3491
PspN4I GGNNCC 7 cut(s) 85, 115, 124, 193, 194, 2841, 3490
PspOMI GGGCCC 3 cut(s) 113, 192, 2839
PspPPI RGGWCCY 2 cut(s) 205, 1198
PstI CTGCAG 2 cut(s) 1038, 2551
PstNI CAGNNNCTG 2 cut(s) 1054, 3600
PsuI RGATCY 2 cut(s) 498, 1051
PvuII CAGCTG 5 cut(s) 280, 1033, 1872, 2422, 2740
RsaI GTAC 6 cut(s) 341, 377, 1783, 1948, 3104, 3254
RsaNI GTAC 6 cut(s) 340, 376, 1782, 1947, 3103, 3253
RseI CAYNNNNRTG 3 cut(s) 186, 1353, 3260
SacI GAGCTC 2 cut(s) 45, 300
SapI GCTCTTC 1 cut(s) 2037
Sau3AI GATC 9 cut(s) 210, 498, 667, 1051, 1222, 1794, 2206, 2587, 3229
SchI GAGTC 6 cut(s) 458, 1525, 2039, 2087, 2100, 2732
ScrFI CCNGG 8 cut(s) 11, 38, 418, 1202, 1510, 2462, 2987, 3493
SduI GDGCHC 5 cut(s) 45, 117, 196, 300, 2843
SfaNI GCATC 9 cut(s) 1147, 1630, 1689, 2055, 2157, 2929, 3109, 3412, 3565
SfcI CTRYAG 5 cut(s) 1034, 1579, 2244, 2547, 2673
SfoI GGCGCC 1 cut(s) 124
Sfr274I CTCGAG 1 cut(s) 44
SfuI TTCGAA 1 cut(s) 1144
SinI GGWCC 4 cut(s) 205, 238, 1198, 2989
SlaI CTCGAG 1 cut(s) 44
SmiI ATTTAAAT 1 cut(s) 3210
SmiMI CAYNNNNRTG 3 cut(s) 186, 1353, 3260
SmlI CTYRAG 8 cut(s) 44, 200, 491, 518, 2228, 2276, 2372, 2912
SmoI CTYRAG 8 cut(s) 44, 200, 491, 518, 2228, 2276, 2372, 2912
SphI GCATGC 1 cut(s) 1388
SseBI AGGCCT 1 cut(s) 3391
SspDI GGCGCC 1 cut(s) 122
SspMI CTAG 5 cut(s) 809, 1067, 2048, 3048, 3431
SstI GAGCTC 2 cut(s) 45, 300
StuI AGGCCT 1 cut(s) 3391
StyD4I CCNGG 8 cut(s) 9, 36, 416, 1200, 1508, 2460, 2985, 3491
StyI CCWWGG 3 cut(s) 471, 1623, 2169
SwaI ATTTAAAT 1 cut(s) 3210
TaaI ACNGT 9 cut(s) 344, 1263, 1553, 1562, 1618, 1903, 1980, 3319, 3399
TaiI ACGT 2 cut(s) 368, 3484
TaqI TCGA 8 cut(s) 45, 222, 348, 1144, 1527, 2704, 2850, 3420
TatI WGTACW 2 cut(s) 375, 3252
TauI GCSGC 3 cut(s) 252, 774, 3309
TfiI GAWTC 8 cut(s) 403, 1146, 1505, 2307, 2471, 2495, 2825, 3462
TscAI CASTG 5 cut(s) 106, 1985, 2597, 3195, 3262
TseFI GTSAC 3 cut(s) 361, 2724, 2745
TseI GCWGC 9 cut(s) 333, 1033, 2211, 2546, 2549, 2561, 2737, 3323, 3425
Tsp45I GTSAC 3 cut(s) 361, 2724, 2745
TspGWI ACGGA 5 cut(s) 176, 647, 1730, 2339, 3458
TspRI CASTG 5 cut(s) 106, 1985, 2597, 3195, 3262
Van91I CCANNNNNTGG 2 cut(s) 2389, 3194
Vha464I CTTAAG 1 cut(s) 2228
VpaK11BI GGWCC 4 cut(s) 205, 238, 1198, 2989
XceI RCATGY 2 cut(s) 1220, 1388
XhoI CTCGAG 1 cut(s) 44
XmnI GAANNNNTTC 3 cut(s) 1140, 1254, 2529
XspI CTAG 5 cut(s) 809, 1067, 2048, 3048, 3431
Zsp2I ATGCAT 2 cut(s) 1682, 3375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.