RchiOBHm_Chr6g0287971

Structural maintenance of chromosomes protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006326
Physical Location & Seq
Forward (+)
51266273 .. 51276716
10444 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25837

Sequence Viewer

Length: 4041 bp
ATGCCGTCCCTGATCTCCCAGGGCAAAATCCACCGCCTGGAGCTCGAGAATTTCAAGTCCTACCGGGGCCACCAGGTCATCGGCCCCTTCTCCGACTTCACGGCGATTATCGGCCCCAATGGCTCCGGCAAGTCCAACCTCATGGACGCCATAAGCTTCGTCCTTGGGGTCAAGACCGGCCAGCTCCGTGGGTCCCAGTTGAAGGACCTGATCTACGCCATGGACGACAGTGAGAAGAGCGAGAAAGGCCGCAGGGCGTTTGTGAGCTTGGTTTACCAGCTTGCGAATGAGTCTGAGATCCAGTTCACAAGGGCCATCACTAGCTCCGGCGGCAGTGAGTACCGCATAGACGGCAAGTCGGTATCGTGGGAAGCTTACAATGAGAAGCTTAAGTCCCTTGGAATTCTAGTCAAGGCTCGCAATTTTCTTGTCTTTCAGGGTGATGTCGAGTCCATTGCCTCTAAAAATCCTAAGGAACTCACTGGACTGCTTGAGCAGATCTCAGGGTCTGATGAGCTCAAGAGAGACTATGAAAAGTATGAAGAAGAGAAAGGCATAGCTGAAGAAAAAGCAGCCCTTGTTTATCAGAAAAAAAGAACCATAGTAGCAGAGAGGAAGCAAAAGAAAGAGCAAAAGGAAGAAGCTGAGAAGCACATTCGCCTGCAAAATGAACTGAAATCTTTGAAGAGAGAACACTTTTTGTGGCAGTTGTTTAACATAGAAAAAGATATAACGAAAACAACTGATGAGCTTGAAGCTGAAAAGAGAAGCCGCGAACAGGTTATGCAAGAACTAGATGATTTTCAGCAAGAAGCAAACAAAAAGAAGAAAGAACTTGCCAAATACTTAAAAGAGATTGCTCAATGTGAAAAGAAGATTGCTGAGAGAAGTAGTAGACTTGACAGAAGTAAACCAGAGCTGCTGAAACTGAAAGAGGAAATGTCGCGAATAAATTCAAAAATCAAGAAAAGTAAGACAGAGCTTGGTAGAAAAGAACAAGAACGGGGGAGACATAAGGAGGAAATTAAAAAGCTGCAGAAGGGCATCCAGGATTTGACCACACAACTAGAGGATTTACAGGAAAAAGGCCGAGACAGTGGTGAAAAATTACATTTAGACGATACTAAATTAAGGGAATATTTTAAAGTGAAGGAGGATGCTGGGATGAAAACTGCAAAGCTAAAAGATGAGAAAGAGGTTCTGGATCGGCAACAGCATGCTGATCTTGAAGCTCAAAAGAATTTGGAAGAAAATCTTCAACAGTTGAGAAGTAGGGAGCGTGAACTGGATTCACAGAATGAGCAAATGCTGACAAGGCAGAGAAAAATTAAGGAAAACTCTGCAAAACACAAGCAGGAATCTAAAAATCTGAATAATGAATTGCATGTGATGCAGGAGAAACATCAGAATGCCAGACAAAAGTATGAAAATCTGAAGTCAAAAATTGATGAATTAGAAAAGCAATTACGTGAACTGAAGGCCGACAGATATGAAAATGAGAGAGATTCCAGGTTGTCTCAGGCAGTGGAGACGCTCAAGCGCTTGTTTCAGGGTGTCCATGGTCGCATGACTGAACTCTGTAGGCCAACACAAAAGAAGTATAATCTTGCTGTCACTGTTGCCATGGGGAAATTTATGGATGCAGTCGTAGTTGAAGATGAACAAACAGGAAAGGAGTGTATCAAGTATTTGAAAGAACAAAGGCTTCCTCCCCAGACATTCATACCTCTCCAATCTGTTCGTGTAAAGCAAGTAATGGAGAGATTGCGTAACTTGGGTGGTACTGCAAAGCTGGTTTTTGATGTTTGCATATTTTGTTGCTCTTTATTTGTATGGATAGGAATAGGCAGAGACATTATGTGTCTGATTGATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATTACATGTCATGTTTTGCAGCATTGGGAGCATCTTTGATCTGGGATGGTTTGTGCTGCTAATTTTTGGTGCATTCACGAGTTTTCTTTATGCAACTCCTCACTCCTGTGGCCCTCTCCCACAAAGAAGTTTAAGTTTTCAACTGATAATAATCATCCACATTAGTATCATCACTGCATTTATTTTGGTGAACATGTTAATGCATGGAAGCATCAAGCGGACACCTTCACCATTTCATGTTTGTCTACTTAACAACCAAACATTTGATCATGCTTTGGAGAAGGCTATTCTTTTTGCGGTTGGCAATACACTTGTTTGTGACGAACTTGATGAGGCTAAGCGCCTAAGCTGGAGTGGTGAGAGGTTCAAAGTTGTAACTGTTGATGGAATTATGCTGTCAAAATCTGGCACAATGACTGGGGGTACCAGTGGAGGAATGGAAGCTAGATCAAAACAATGGGATGATAAAAAAGTTGAAGGACTGAAGAAGAAGAAAGAACAGTTTGAGTTGGAGCTAGAAGAGCTTGGGTCAATTAGAGAGATGCAGCTAAAAGAGTCTGAAACAGCTGGCAGAATTAGTGGGCTTGACAAGAAAATCCAATATGCAGATATTGAGAAGGAAAGCATAAAGGACAAACTTGCAAATTTGGTCCACGAGAGGCAGAATATAAAAGAAGAGATTGATCGTATCAGTCCTGATCTTCTGAAGCTAAAACAAGCTGTAGATAAGCGGAGCACAGAAATCAATAAGCTTGAGAAGAGGATAAATGATATTGTTGACAGACTCTACAAAGGCTTTAGTAAATCTGTTGGGGTAGATAACATTCGCGAGTATGAAGAAAAACAACTCAAGGCTACCCAATCTATGGCTGAAGAGAGGCTTAGTTTGAGTAGCCAGCTATCAAAACTGAAATACCAGCTAGAGTATGAGCAAAATCGGGACATGGATACACGAATTGAAGAACTCCAATCATTTATTAGTAACCTACTAAAAGATTTAGAACGGGTTCAAAAGAAAGAGGCCGAAGCCAACTCTGCAGCAGAAAAAGCCTCTGATGAGATTCTTCAGTTGAAGGAAGAAGCACAAGAATGGAAGTCCAAGTCGGAAGGGTGTGAAAAGGAAATTCAAGAATGGAACAAGCGGGGTTCTACTGCAACATCAAATGTATCCAAACTTAATCGACAGATAAATTCTAAGGAATCGCAGATTGAGCAGCTAACATCTCGAAAGCAGGAGATAGTAGAAAATTGTGAACTACAGCAAATAAGCCTTCCGATCATTTCAGATCCAATGGAGACTGAATCCTCCACGACAGGCCCAGTATTTGATTTTGATGAGTTAGATGAGTCTCTATTGAGGAATAGGAGGCCTTCTGAGCGGGAGAAAGTTGAGTTAGACTTTAAGAAACAAATGGATGCCAAGTTATCAGAAATTGAAAGAACAGCTCCCAATTTGAAGGCCATGGACCAATATGAGGCTCTACAAGAAAAGGAAAGAGATGTAACTGGAGAGTTTGAAGTGGCCAGAAAAGAACAGAAGCAAAAGGCTGATCTGTTTAACTCGGTTAAGCAAAGGAGGTATGAGTTGTTTATGGATGCCTTCAACCATATTTCCAGTAATATTGATAAGATATACAAACAACTGACGAAGAGCAATACACATCCCCTGGGTGGGACGGCATATTTGAATTTAGAAAATGAAGATGATCCCTTTTTACATGGCGTCAAGTACACTACCATGCCCCCAACAAAGCGCTTTCGAGATATGGAACAATTATCTGGCGGAGAGAAAACTGTTGCAGCGTTAGCATTACTGTTTTCCATACACAGCTATAGGCCTTCGCCTTTTTTCATACTGGATGAAGTTGATGCTGCACTGGATAACTTAAATGTAGCAAAGGTTGCTCGATTCATCCGTTCAAAGTCATGCCAAGGAGCCAGGGTTAATCAGGATACTGAAGGAGGCAATGGTTTTCAAAGTATTGTAATATCTCTAAAAGATAGCTTCTATGACAAGGCTGAAGCTCTGGTTGGGGTTTTCAGGGACGCTGACATGGGTTGTTCCAAAACACTGTCATTTGACTTGACTAAATTTCGGGAGGAGTAA

Protein Analysis

1346

Amino Acids

155.46

Weight (kDa)

7.5

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_15 PF13175 9 - 356 4.5e-14 AAA ATPase domain
SMC_N PF02463 10 - 1327 9.9e-56 RecF/RecN/SMC N terminal domain
AAA_29 PF13555 11 - 55 4.4e-08 P-loop containing region of AAA domain
AAA_23 PF13476 12 - 219 4.4e-16 AAA domain
AAA_21 PF13304 33 - 61 1.7e-07 AAA domain, putative AbiEii toxin, Type IV TA system
SMC_hinge PF06470 517 - 585 2.6e-15 SMC proteins Flexible Hinge Domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2357
AccB1I GGYRCC 1 cut(s) 2357
AccB7I CCANNNNNTGG 2 cut(s) 37, 2800
AccBSI CCGCTC 1 cut(s) 3313
AccI GTMKAC 2 cut(s) 895, 2179
AccII CGCG 3 cut(s) 774, 946, 2763
AclWI GGATC 4 cut(s) 292, 1212, 3215, 3635
AcoI YGGCCR 2 cut(s) 178, 3456
AcuI CTGAAG 9 cut(s) 582, 1454, 1496, 2438, 2660, 2826, 2984, 3912, 3975
AcyI GRCGYC 2 cut(s) 147, 3657
AfaI GTAC 4 cut(s) 341, 1783, 2359, 3665
AfeI AGCGCT 2 cut(s) 1541, 3689
AfiI CCNNNNNNNGG 7 cut(s) 37, 202, 778, 2800, 3409, 3605, 3606
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 2 cut(s) 1939, 2127
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 7 cut(s) 18, 36, 72, 1047, 1508, 3600, 3872
AjuI GAANNNNNNNTTGG 2 cut(s) 3948, 3980
AleI CACNNNNGTG 1 cut(s) 2040
Alw21I GWGCWC 3 cut(s) 45, 519, 2672
Alw26I GTCTC 8 cut(s) 519, 1003, 1086, 1521, 1523, 1845, 3224, 3288
AlwI GGATC 4 cut(s) 292, 1212, 3215, 3635
AlwNI CAGNNNCTG 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 44
Aor51HI AGCGCT 2 cut(s) 1541, 3689
ArsI GACNNNNNNTTYG 2 cut(s) 3601, 3633
Asp700I GAANNNNTTC 3 cut(s) 952, 1254, 2940
Asp718I GGTACC 1 cut(s) 2357
AspLEI GCGC 3 cut(s) 1542, 2277, 3690
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 5 cut(s) 452, 1112, 2134, 2154, 2303
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 4 cut(s) 192, 205, 2584, 3400
AxyI CCTNAGG 1 cut(s) 471
BaeI ACNNNNGTAYC 2 cut(s) 2083, 2116
BalI TGGCCA 1 cut(s) 3458
BanI GGYRCC 1 cut(s) 2357
BanII GRGCYC 2 cut(s) 45, 519
BauI CACGAG 2 cut(s) 2011, 2588
Bbv12I GWGCWC 3 cut(s) 45, 519, 2672
BccI CCATC 3 cut(s) 323, 1974, 2312
BceAI ACGGC 3 cut(s) 117, 367, 3627
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 7 cut(s) 20, 38, 74, 1049, 1510, 3602, 3874
BciVI GTATCC 3 cut(s) 2875, 3112, 3880
BclI TGATCA 1 cut(s) 2200
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 8 cut(s) 519, 1003, 1086, 1521, 1523, 1845, 3224, 3288
BfaI CTAG 7 cut(s) 321, 407, 794, 1067, 2379, 2450, 2855
BfmI CTRYAG 6 cut(s) 1034, 1579, 2655, 2970, 3191, 3766
BfoI RGCGCY 3 cut(s) 1543, 2278, 3691
BfrI CTTAAG 1 cut(s) 389
BfuI GTATCC 3 cut(s) 2875, 3112, 3880
BglI GCCNNNNNGGC 1 cut(s) 120
BglII AGATCT 1 cut(s) 498
BlpI GCTNAGC 1 cut(s) 2271
Bme1390I CCNGG 8 cut(s) 20, 38, 65, 74, 1049, 1510, 3602, 3874
Bme18I GGWCC 4 cut(s) 192, 205, 2584, 3400
BmeRI GACNNNNNGTC 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 8 cut(s) 68, 85, 115, 124, 193, 194, 2359, 3871
BmrFI CCNGG 8 cut(s) 20, 38, 65, 74, 1049, 1510, 3602, 3874
BmrI ACTGGG 3 cut(s) 190, 2361, 3248
BmuI ACTGGG 3 cut(s) 190, 2361, 3248
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 3 cut(s) 59, 2305, 3462
Bpu10I CCTNAGC 1 cut(s) 2279
Bpu1102I GCTNAGC 1 cut(s) 2271
BpuEI CTTGAG 5 cut(s) 503, 512, 1520, 2708, 2768
BpuMI CCSGG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 1469
BsaBI GATNNNNATC 1 cut(s) 2084
BsaHI GRCGYC 2 cut(s) 147, 3657
BsaXI ACNNNNNCTCC 2 cut(s) 3861, 3891
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 202, 778, 2800, 3409, 3605, 3606
Bse118I RCCGGY 1 cut(s) 176
Bse21I CCTNAGG 1 cut(s) 471
Bse3DI GCAATG 2 cut(s) 453, 3907
Bse8I GATNNNNATC 1 cut(s) 2084
BseBI CCWGG 7 cut(s) 20, 38, 74, 1049, 1510, 3602, 3874
BseJI GATNNNNATC 1 cut(s) 2084
BseLI CCNNNNNNNGG 7 cut(s) 37, 202, 778, 2800, 3409, 3605, 3606
BseMI GCAATG 2 cut(s) 453, 3907
BseMII CTCAG 6 cut(s) 285, 516, 636, 873, 1532, 3300
BseRI GAGGAG 1 cut(s) 2022
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 1 cut(s) 3792
Bsh1236I CGCG 3 cut(s) 774, 946, 2763
BshNI GGYRCC 1 cut(s) 2357
BsiHKAI GWGCWC 3 cut(s) 45, 519, 2672
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 4 cut(s) 64, 126, 177, 327
BslFI GGGAC 5 cut(s) 178, 379, 2888, 3622, 3992
BslI CCNNNNNNNGG 7 cut(s) 37, 202, 778, 2800, 3409, 3605, 3606
BsmAI GTCTC 8 cut(s) 519, 1003, 1086, 1521, 1523, 1845, 3224, 3288
BsmBI CGTCTC 1 cut(s) 1523
BsmFI GGGAC 5 cut(s) 178, 379, 2888, 3622, 3992
BsmI GAATGC 2 cut(s) 1414, 2006
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 3 cut(s) 45, 519, 2672
Bsp1720I GCTNAGC 1 cut(s) 2271
Bsp19I CCATGG 4 cut(s) 219, 1558, 1623, 3396
Bsp68I TCGCGA 2 cut(s) 946, 2763
BspCNI CTCAG 6 cut(s) 286, 515, 637, 874, 1531, 3301
BspFNI CGCG 3 cut(s) 774, 946, 2763
BspLI GGNNCC 8 cut(s) 68, 85, 115, 124, 193, 194, 2359, 3871
BspMAI CTGCAG 2 cut(s) 1038, 2974
BspPI GGATC 4 cut(s) 292, 1212, 3215, 3635
BspQI GCTCTTC 3 cut(s) 230, 2448, 3578
BspT107I GGYRCC 1 cut(s) 2357
BspTI CTTAAG 1 cut(s) 389
BsrBI CCGCTC 1 cut(s) 3313
BsrDI GCAATG 2 cut(s) 453, 3907
BsrFI RCCGGY 1 cut(s) 176
BssAI RCCGGY 1 cut(s) 176
BssNI GRCGYC 2 cut(s) 147, 3657
BssSI CACGAG 2 cut(s) 2011, 2588
BssT1I CCWWGG 7 cut(s) 163, 219, 397, 1558, 1623, 3396, 3865
Bst2BI CACGAG 2 cut(s) 2011, 2588
Bst2UI CCWGG 7 cut(s) 20, 38, 74, 1049, 1510, 3602, 3874
Bst4CI ACNGT 9 cut(s) 230, 1097, 1263, 1618, 2314, 2436, 3730, 3750, 4008
Bst6I CTCTTC 8 cut(s) 230, 540, 680, 2448, 2604, 2687, 2802, 3578
BstACI GRCGYC 2 cut(s) 147, 3657
BstAFI CTTAAG 1 cut(s) 389
BstAPI GCANNNNNTGC 2 cut(s) 1390, 3836
BstBAI YACGTR 1 cut(s) 1469
BstC8I GCNNGC 7 cut(s) 182, 282, 418, 662, 1218, 2503, 2831
BstDSI CCRYGG 5 cut(s) 187, 219, 1558, 1623, 3396
BstFNI CGCG 3 cut(s) 774, 946, 2763
BstH2I RGCGCY 3 cut(s) 1543, 2278, 3691
BstHHI GCGC 3 cut(s) 1542, 2277, 3690
BstMAI GTCTC 8 cut(s) 519, 1003, 1086, 1521, 1523, 1845, 3224, 3288
BstNI CCWGG 7 cut(s) 20, 38, 74, 1049, 1510, 3602, 3874
BstNSI RCATGY 4 cut(s) 1220, 1388, 1943, 2131
BstSCI CCNGG 8 cut(s) 18, 36, 63, 72, 1047, 1508, 3600, 3872
BstSFI CTRYAG 6 cut(s) 1034, 1579, 2655, 2970, 3191, 3766
BstUI CGCG 3 cut(s) 774, 946, 2763
BstX2I RGATCY 3 cut(s) 297, 498, 3220
BstXI CCANNNNNNTGG 2 cut(s) 142, 188
BstYI RGATCY 3 cut(s) 297, 498, 3220
Bsu36I CCTNAGG 1 cut(s) 471
BsuI GTATCC 3 cut(s) 2875, 3112, 3880
BtgI CCRYGG 5 cut(s) 187, 219, 1558, 1623, 3396
BtsI GCAGTG 3 cut(s) 340, 1530, 2106
BtuMI TCGCGA 2 cut(s) 946, 2763
Cac8I GCNNGC 7 cut(s) 182, 282, 418, 662, 1218, 2503, 2831
CaiI CAGNNNCTG 1 cut(s) 509
CfoI GCGC 3 cut(s) 1542, 2277, 3690
Cfr10I RCCGGY 1 cut(s) 176
CseI GACGC 4 cut(s) 155, 1540, 3646, 3989
CsiI ACCWGGT 1 cut(s) 72
Csp6I GTAC 4 cut(s) 340, 1782, 2358, 3664
CviQI GTAC 4 cut(s) 340, 1782, 2358, 3664
DraI TTTAAA 1 cut(s) 1144
DriI GACNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 2 cut(s) 178, 3456
Eam1104I CTCTTC 8 cut(s) 230, 540, 680, 2448, 2604, 2687, 2802, 3578
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 8 cut(s) 230, 540, 680, 2448, 2604, 2687, 2802, 3578
EciI GGCGGA 1 cut(s) 3732
Ecl136II GAGCTC 2 cut(s) 43, 517
Eco130I CCWWGG 7 cut(s) 163, 219, 397, 1558, 1623, 3396, 3865
Eco147I AGGCCT 2 cut(s) 3304, 3772
Eco24I GRGCYC 2 cut(s) 45, 519
Eco47I GGWCC 4 cut(s) 192, 205, 2584, 3400
Eco47III AGCGCT 2 cut(s) 1541, 3689
Eco53kI GAGCTC 2 cut(s) 43, 517
Eco57I CTGAAG 9 cut(s) 582, 1454, 1496, 2438, 2660, 2826, 2984, 3912, 3975
Eco81I CCTNAGG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 44
EcoICRI GAGCTC 2 cut(s) 43, 517
EcoO109I RGGNCCY 2 cut(s) 192, 205
EcoRI GAATTC 1 cut(s) 402
EcoRII CCWGG 7 cut(s) 18, 36, 72, 1047, 1508, 3600, 3872
EcoT14I CCWWGG 7 cut(s) 163, 219, 397, 1558, 1623, 3396, 3865
EcoT22I ATGCAT 1 cut(s) 2139
EcoT38I GRGCYC 2 cut(s) 45, 519
ErhI CCWWGG 7 cut(s) 163, 219, 397, 1558, 1623, 3396, 3865
Esp3I CGTCTC 1 cut(s) 1523
FalI AAGNNNNNCTT 6 cut(s) 561, 593, 1239, 1271, 2799, 2831
FaqI GGGAC 5 cut(s) 178, 379, 2888, 3622, 3992
FauI CCCGC 2 cut(s) 3069, 3306
FbaI TGATCA 1 cut(s) 2200
FblI GTMKAC 2 cut(s) 895, 2179
FriOI GRGCYC 2 cut(s) 45, 519
FspBI CTAG 7 cut(s) 321, 407, 794, 1067, 2379, 2450, 2855
GlaI GCGC 3 cut(s) 1541, 2276, 3689
GsaI CCCAGC 1 cut(s) 1164
GsuI CTGGAG 3 cut(s) 59, 2305, 3462
HaeII RGCGCY 3 cut(s) 1543, 2278, 3691
HapII CCGG 4 cut(s) 64, 126, 177, 327
HgaI GACGC 4 cut(s) 155, 1540, 3646, 3989
HhaI GCGC 3 cut(s) 1542, 2277, 3690
Hin1I GRCGYC 2 cut(s) 147, 3657
Hin6I GCGC 3 cut(s) 1540, 2275, 3688
HinP1I GCGC 3 cut(s) 1540, 2275, 3688
HincII GTYRAC 1 cut(s) 2713
HindII GTYRAC 1 cut(s) 2713
HindIII AAGCTT 4 cut(s) 154, 372, 386, 2684
HpaII CCGG 4 cut(s) 64, 126, 177, 327
HphI GGTGA 5 cut(s) 452, 1112, 2134, 2154, 2303
HpyCH4III ACNGT 9 cut(s) 230, 1097, 1263, 1618, 2314, 2436, 3730, 3750, 4008
HpyCH4IV ACGT 1 cut(s) 1468
HpySE526I ACGT 1 cut(s) 1468
Hsp92I GRCGYC 2 cut(s) 147, 3657
HspAI GCGC 3 cut(s) 1540, 2275, 3688
KflI GGGWCCC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 2361
Ksp22I TGATCA 1 cut(s) 2200
LguI GCTCTTC 3 cut(s) 230, 2448, 3578
MabI ACCWGGT 1 cut(s) 72
MaeI CTAG 7 cut(s) 321, 407, 794, 1067, 2379, 2450, 2855
MaeII ACGT 1 cut(s) 1468
MaeIII GTNAC 6 cut(s) 1612, 1769, 2252, 2308, 2915, 3436
MbiI CCGCTC 1 cut(s) 3313
MflI RGATCY 3 cut(s) 297, 498, 3220
MhlI GDGCHC 3 cut(s) 45, 519, 2672
MlsI TGGCCA 1 cut(s) 3458
MluNI TGGCCA 1 cut(s) 3458
MlyI GAGTC 5 cut(s) 299, 458, 2498, 2712, 3290
MmeI TCCRAC 4 cut(s) 117, 159, 2424, 3018
Mox20I TGGCCA 1 cut(s) 3458
Mph1103I ATGCAT 1 cut(s) 2139
MroXI GAANNNNTTC 3 cut(s) 952, 1254, 2940
MscI TGGCCA 1 cut(s) 3458
MslI CAYNNNNRTG 5 cut(s) 1407, 2040, 2132, 3022, 3671
Msp20I TGGCCA 1 cut(s) 3458
MspA1I CMGCKG 1 cut(s) 2501
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 4 cut(s) 64, 126, 177, 327
MspR9I CCNGG 8 cut(s) 20, 38, 65, 74, 1049, 1510, 3602, 3874
Mva1269I GAATGC 2 cut(s) 1414, 2006
MvaI CCWGG 7 cut(s) 20, 38, 74, 1049, 1510, 3602, 3874
MvnI CGCG 3 cut(s) 774, 946, 2763
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 4 cut(s) 219, 1558, 1623, 3396
NlaIV GGNNCC 8 cut(s) 68, 85, 115, 124, 193, 194, 2359, 3871
NmeAIII GCCGAG 1 cut(s) 1115
NmuCI GTSAC 2 cut(s) 1612, 2252
NruI TCGCGA 2 cut(s) 946, 2763
NsiI ATGCAT 1 cut(s) 2139
NspI RCATGY 4 cut(s) 1220, 1388, 1943, 2131
OliI CACNNNNGTG 1 cut(s) 2040
PaeI GCATGC 1 cut(s) 1220
PaeR7I CTCGAG 1 cut(s) 44
PasI CCCWGGG 2 cut(s) 19, 3601
PceI AGGCCT 2 cut(s) 3304, 3772
PciI ACATGT 2 cut(s) 1939, 2127
PciSI GCTCTTC 3 cut(s) 230, 2448, 3578
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 3847
PctI GAATGC 2 cut(s) 1414, 2006
PdmI GAANNNNTTC 3 cut(s) 952, 1254, 2940
PfeI GAWTC 7 cut(s) 1289, 1358, 1505, 2995, 3134, 3236, 3843
PflMI CCANNNNNTGG 2 cut(s) 37, 2800
PfoI TCCNGGA 1 cut(s) 1047
PleI GAGTC 5 cut(s) 298, 457, 2497, 2712, 3289
PpsI GAGTC 5 cut(s) 298, 457, 2497, 2712, 3289
Ppu21I YACGTR 1 cut(s) 1469
PpuMI RGGWCCY 2 cut(s) 192, 205
PscI ACATGT 2 cut(s) 1939, 2127
Psp124BI GAGCTC 2 cut(s) 45, 519
Psp5II RGGWCCY 2 cut(s) 192, 205
Psp6I CCWGG 7 cut(s) 18, 36, 72, 1047, 1508, 3600, 3872
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 7 cut(s) 18, 36, 72, 1047, 1508, 3600, 3872
PspN4I GGNNCC 8 cut(s) 68, 85, 115, 124, 193, 194, 2359, 3871
PspPPI RGGWCCY 2 cut(s) 192, 205
PstI CTGCAG 2 cut(s) 1038, 2974
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 3 cut(s) 297, 498, 3220
PvuII CAGCTG 1 cut(s) 2501
RruI TCGCGA 2 cut(s) 946, 2763
RsaI GTAC 4 cut(s) 341, 1783, 2359, 3665
RsaNI GTAC 4 cut(s) 340, 1782, 2358, 3664
RseI CAYNNNNRTG 5 cut(s) 1407, 2040, 2132, 3022, 3671
SacI GAGCTC 2 cut(s) 45, 519
SapI GCTCTTC 3 cut(s) 230, 2448, 3578
SchI GAGTC 5 cut(s) 299, 458, 2498, 2712, 3290
ScrFI CCNGG 8 cut(s) 20, 38, 65, 74, 1049, 1510, 3602, 3874
SduI GDGCHC 3 cut(s) 45, 519, 2672
SexAI ACCWGGT 1 cut(s) 72
SfcI CTRYAG 6 cut(s) 1034, 1579, 2655, 2970, 3191, 3766
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 4 cut(s) 192, 205, 2584, 3400
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 5 cut(s) 1407, 2040, 2132, 3022, 3671
SmlI CTYRAG 7 cut(s) 44, 389, 491, 518, 1535, 2687, 2783
SmoI CTYRAG 7 cut(s) 44, 389, 491, 518, 1535, 2687, 2783
SphI GCATGC 1 cut(s) 1220
SseBI AGGCCT 2 cut(s) 3304, 3772
SspI AATATT 2 cut(s) 1139, 3556
SspMI CTAG 7 cut(s) 321, 407, 794, 1067, 2379, 2450, 2855
SstI GAGCTC 2 cut(s) 45, 519
StuI AGGCCT 2 cut(s) 3304, 3772
StyD4I CCNGG 8 cut(s) 18, 36, 63, 72, 1047, 1508, 3600, 3872
StyI CCWWGG 7 cut(s) 163, 219, 397, 1558, 1623, 3396, 3865
TaaI ACNGT 9 cut(s) 230, 1097, 1263, 1618, 2314, 2436, 3730, 3750, 4008
TaiI ACGT 1 cut(s) 1471
TaqI TCGA 6 cut(s) 45, 447, 3115, 3160, 3694, 3841
TatI WGTACW 1 cut(s) 3663
TauI GCSGC 3 cut(s) 252, 333, 774
TfiI GAWTC 7 cut(s) 1289, 1358, 1505, 2995, 3134, 3236, 3843
TseFI GTSAC 2 cut(s) 1612, 2252
Tsp45I GTSAC 2 cut(s) 1612, 2252
TspGWI ACGGA 2 cut(s) 176, 3839
Van91I CCANNNNNTGG 2 cut(s) 37, 2800
Vha464I CTTAAG 1 cut(s) 389
VpaK11BI GGWCC 4 cut(s) 192, 205, 2584, 3400
XceI RCATGY 4 cut(s) 1220, 1388, 1943, 2131
XcmI CCANNNNNNNNNTGG 1 cut(s) 2368
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 895, 2179
XmnI GAANNNNTTC 3 cut(s) 952, 1254, 2940
XspI CTAG 7 cut(s) 321, 407, 794, 1067, 2379, 2450, 2855
Zsp2I ATGCAT 1 cut(s) 2139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.