AT3G56130

Biotin carboxyl carrier protein of acetyl-CoA carboxylase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006727
Physical Location & Seq
Forward (+)
20826576 .. 20829270
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56130.2

Sequence Viewer

Length: 846 bp
ATGGCGTCTTCTGCAGCTCTCGGATCTCTCCATCAGACTTTAGGGTCAGCCATTAATTCACAGAGTGAGGTTCACTCGCTTTCTGGAAACTGGTCTGCCTCTGGTAATTCATGTGTGCCACGGTGGAGATTATCCAACAGGAACAGCAACTACAGGCTCGTGTTACGTGCTAAGGCCGCTAAATCTTCGACAACAACCATAAGTGATGGTTCATCTGATGCTAGTGTGTCAGACGGGAAGAAAACAGTTCGACGGATAACTTTCCCGAAAGAAGTGGAGGCACTGGTTCACGAGATGTGTGATGAAACTGAGGTTGCTGTGCTGCAACTTAAGGTTGGAGATTTCGAGATGAACCTAAAACGGAAGATTGGAGCAGCCACAAACCCCATTCCCGTGGCGGATATATCTCCAACTGTAGCGCCTCCTATTCCTTCTGAACCTATGAATAAATCTGCTTCTTCGGCTCCTAGCCCATCTCAAGCAAAGCCTTCCTCTGAGAAAGTGTCTCCATTTAAGAATACATCATATGGGAAACCAGCAAAGTTGGCTGCTTTGGAGGCATCTGGATCCACCAACTATGTGTTAGTCACATCTCCCGCAGTGGGCAAGTTTCAGAGGAGCAGAACTGTAAAAGGAAAGAAACAATCTCCTAGCTGCAAAGAGGGTGATGCAATAAAGGAAGGCCAAGTTATTGGATACTTACATCAGTTGGGAACAGAACTTCCAGTGACGTCAGATGTAGCTGGAGAAGTCCTTAAGCTTCTTTCAGATGACGGAGACTCCGTAGGTTATGGAGATCCTCTGGTTGCAGTCTTGCCATCTTTCCACGACATCAACATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

281

Amino Acids

29.58

Weight (kDa)

8.86

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_lipoyl PF00364 120 - 194 7.4e-09 Biotin-requiring enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 43
AatII GACGTC 1 cut(s) 734
AciI CCGC 3 cut(s) 177, 398, 597
AclWI GGATC 4 cut(s) 31, 561, 574, 791
AcyI GRCGYC 2 cut(s) 5, 731
AdeI CACNNNGTG 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 602
AflII CTTAAG 2 cut(s) 329, 755
AloI GAACNNNNNNTCC 2 cut(s) 706, 738
AluBI AGCT 4 cut(s) 17, 654, 743, 760
AluI AGCT 4 cut(s) 17, 654, 743, 760
Alw26I GTCTC 2 cut(s) 510, 771
AlwI GGATC 4 cut(s) 31, 561, 574, 791
AoxI GGCC 2 cut(s) 174, 682
ApeKI GCWGC 5 cut(s) 14, 322, 374, 548, 654
AseI ATTAAT 1 cut(s) 54
AspLEI GCGC 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 677
BamHI GGATCC 1 cut(s) 566
BauI CACGAG 2 cut(s) 158, 290
BbvI GCAGC 5 cut(s) 26, 309, 386, 535, 641
BccI CCATC 4 cut(s) 39, 200, 481, 826
BciVI GTATCC 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 510, 771
BfaI CTAG 3 cut(s) 222, 468, 651
BfmI CTRYAG 3 cut(s) 12, 151, 414
BfoI RGCGCY 1 cut(s) 422
BfrI CTTAAG 2 cut(s) 329, 755
BfuI GTATCC 1 cut(s) 689
BisI GCNGC 6 cut(s) 15, 177, 323, 375, 549, 655
BlsI GCNGC 6 cut(s) 16, 178, 324, 376, 550, 656
BmiI GGNNCC 2 cut(s) 465, 568
BmsI GCATC 3 cut(s) 208, 569, 658
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpmI CTGGAG 1 cut(s) 765
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 2 cut(s) 5, 731
BsaJI CCNNGG 2 cut(s) 119, 393
BsaXI ACNNNNNCTCC 4 cut(s) 764, 768, 794, 798
Bsc4I CCNNNNNNNGG 1 cut(s) 602
Bse1I ACTGG 4 cut(s) 95, 288, 725, 841
BseDI CCNNGG 2 cut(s) 119, 393
BseGI GGATG 1 cut(s) 837
BseLI CCNNNNNNNGG 1 cut(s) 602
BseMII CTCAG 2 cut(s) 300, 486
BseNI ACTGG 4 cut(s) 95, 288, 725, 841
BseRI GAGGAG 1 cut(s) 631
BseXI GCAGC 5 cut(s) 26, 309, 386, 535, 641
BshFI GGCC 2 cut(s) 176, 684
BslI CCNNNNNNNGG 1 cut(s) 602
BsmAI GTCTC 2 cut(s) 510, 771
BsnI GGCC 2 cut(s) 176, 684
Bsp143I GATC 3 cut(s) 23, 566, 796
BspACI CCGC 3 cut(s) 177, 398, 597
BspANI GGCC 2 cut(s) 176, 684
BspCNI CTCAG 2 cut(s) 301, 487
BspLI GGNNCC 2 cut(s) 465, 568
BspMAI CTGCAG 1 cut(s) 16
BspPI GGATC 4 cut(s) 31, 561, 574, 791
BspTI CTTAAG 2 cut(s) 329, 755
BsrI ACTGG 4 cut(s) 95, 288, 725, 841
BssECI CCNNGG 2 cut(s) 119, 393
BssMI GATC 3 cut(s) 23, 566, 796
BssNI GRCGYC 2 cut(s) 5, 731
BssSI CACGAG 2 cut(s) 158, 290
Bst2BI CACGAG 2 cut(s) 158, 290
Bst4CI ACNGT 4 cut(s) 123, 247, 415, 628
BstACI GRCGYC 2 cut(s) 5, 731
BstAFI CTTAAG 2 cut(s) 329, 755
BstBAI YACGTR 1 cut(s) 167
BstDEI CTNAG 3 cut(s) 171, 309, 495
BstDSI CCRYGG 2 cut(s) 119, 393
BstF5I GGATG 1 cut(s) 837
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 3 cut(s) 26, 569, 799
BstMAI GTCTC 2 cut(s) 510, 771
BstMBI GATC 3 cut(s) 23, 566, 796
BstMWI GCNNNNNNNGC 5 cut(s) 11, 176, 461, 545, 557
BstSFI CTRYAG 3 cut(s) 12, 151, 414
BstV1I GCAGC 5 cut(s) 26, 309, 386, 535, 641
BstX2I RGATCY 3 cut(s) 23, 566, 796
BstXI CCANNNNNNTGG 2 cut(s) 394, 692
BstYI RGATCY 3 cut(s) 23, 566, 796
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 2 cut(s) 176, 684
BtgI CCRYGG 2 cut(s) 119, 393
BtsCI GGATG 1 cut(s) 837
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 3 cut(s) 281, 606, 732
CfoI GCGC 1 cut(s) 421
CviAII CATG 1 cut(s) 111
DdeI CTNAG 3 cut(s) 171, 309, 495
DpnI GATC 3 cut(s) 25, 568, 798
DpnII GATC 3 cut(s) 23, 566, 796
DraIII CACNNNGTG 1 cut(s) 65
DrdI GACNNNNNNGTC 1 cut(s) 43
DseDI GACNNNNNNGTC 1 cut(s) 43
EciI GGCGGA 1 cut(s) 413
FaeI CATG 1 cut(s) 114
FaiI YATR 8 cut(s) 112, 200, 404, 443, 526, 528, 579, 792
FatI CATG 1 cut(s) 110
FauI CCCGC 1 cut(s) 604
FauNDI CATATG 1 cut(s) 526
Fnu4HI GCNGC 6 cut(s) 15, 177, 323, 375, 549, 655
FokI GGATG 1 cut(s) 824
Fsp4HI GCNGC 6 cut(s) 15, 177, 323, 375, 549, 655
FspBI CTAG 3 cut(s) 222, 468, 651
GlaI GCGC 1 cut(s) 420
GluI GCNGC 6 cut(s) 15, 177, 323, 375, 549, 655
GsuI CTGGAG 1 cut(s) 765
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 2 cut(s) 176, 684
HhaI GCGC 1 cut(s) 421
Hin1I GRCGYC 2 cut(s) 5, 731
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 1 cut(s) 779
HphI GGTGA 1 cut(s) 677
Hpy166II GTNNAC 2 cut(s) 73, 289
Hpy188I TCNGA 9 cut(s) 23, 36, 217, 232, 436, 496, 615, 736, 769
Hpy188III TCNNGA 5 cut(s) 84, 265, 290, 346, 564
Hpy8I GTNNAC 2 cut(s) 73, 289
Hpy99I CGWCG 1 cut(s) 255
HpyAV CCTTC 3 cut(s) 441, 498, 674
HpyCH4III ACNGT 4 cut(s) 123, 247, 415, 628
HpyCH4IV ACGT 2 cut(s) 166, 731
HpyCH4V TGCA 5 cut(s) 14, 325, 657, 671, 809
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 176, 461, 545, 557
HpyF3I CTNAG 3 cut(s) 171, 309, 495
HpySE526I ACGT 2 cut(s) 166, 731
Hsp92I GRCGYC 2 cut(s) 5, 731
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 1 cut(s) 419
Kzo9I GATC 3 cut(s) 23, 566, 796
LmnI GCTCC 3 cut(s) 371, 469, 618
Lsp1109I GCAGC 5 cut(s) 26, 309, 386, 535, 641
LweI GCATC 3 cut(s) 208, 569, 658
MaeI CTAG 3 cut(s) 222, 468, 651
MaeII ACGT 2 cut(s) 166, 731
MaeIII GTNAC 3 cut(s) 162, 586, 727
MalI GATC 3 cut(s) 25, 568, 798
MboI GATC 3 cut(s) 23, 566, 796
MboII GAAGA 4 cut(s) 177, 250, 376, 450
MflI RGATCY 3 cut(s) 23, 566, 796
MluCI AATT 2 cut(s) 55, 106
MlyI GAGTC 1 cut(s) 773
MmeI TCCRAC 3 cut(s) 159, 316, 434
MseI TTAA 4 cut(s) 54, 330, 513, 756
MslI CAYNNNNRTG 1 cut(s) 392
MspCI CTTAAG 2 cut(s) 329, 755
MwoI GCNNNNNNNGC 5 cut(s) 11, 176, 461, 545, 557
NdeI CATATG 1 cut(s) 526
NdeII GATC 3 cut(s) 23, 566, 796
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 2 cut(s) 465, 568
NmuCI GTSAC 2 cut(s) 586, 727
PcsI WCGNNNNNNNCGW 1 cut(s) 780
PkrI GCNGC 6 cut(s) 16, 178, 324, 376, 550, 656
PleI GAGTC 1 cut(s) 773
PpsI GAGTC 1 cut(s) 773
Ppu21I YACGTR 1 cut(s) 167
PshBI ATTAAT 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 465, 568
PsrI GAACNNNNNNTAC 2 cut(s) 134, 166
PstI CTGCAG 1 cut(s) 16
PsuI RGATCY 3 cut(s) 23, 566, 796
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 4 cut(s) 54, 330, 513, 756
SatI GCNGC 6 cut(s) 15, 177, 323, 375, 549, 655
Sau3AI GATC 3 cut(s) 23, 566, 796
SchI GAGTC 1 cut(s) 773
SfaNI GCATC 3 cut(s) 208, 569, 658
SfcI CTRYAG 3 cut(s) 12, 151, 414
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 3 cut(s) 329, 477, 755
SmoI CTYRAG 3 cut(s) 329, 477, 755
Sse9I AATT 2 cut(s) 55, 106
SsiI CCGC 3 cut(s) 177, 398, 597
SspMI CTAG 3 cut(s) 222, 468, 651
TaaI ACNGT 4 cut(s) 123, 247, 415, 628
TaiI ACGT 2 cut(s) 169, 734
TaqI TCGA 3 cut(s) 188, 250, 345
TasI AATT 2 cut(s) 55, 106
TauI GCSGC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 54, 330, 513, 756
Tru9I TTAA 4 cut(s) 54, 330, 513, 756
TscAI CASTG 3 cut(s) 288, 606, 732
TseFI GTSAC 2 cut(s) 586, 727
TseI GCWGC 5 cut(s) 14, 322, 374, 548, 654
Tsp45I GTSAC 2 cut(s) 586, 727
TspDTI ATGAA 5 cut(s) 99, 201, 318, 365, 458
TspGWI ACGGA 4 cut(s) 268, 376, 772, 789
TspRI CASTG 3 cut(s) 288, 606, 732
Vha464I CTTAAG 2 cut(s) 329, 755
VspI ATTAAT 1 cut(s) 54
XspI CTAG 3 cut(s) 222, 468, 651
ZraI GACGTC 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.