RchiOBHm_Chr1g0371301

Biotin carboxyl carrier protein of acetyl-CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006727
Physical Location & Seq
Forward (+)
60568397 .. 60573093
4697 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59539

Sequence Viewer

Length: 873 bp
ATGGAGTCCTCTGCCGTTCTCCGATCCTTCAACTGTTCTGTTGGCACAGTTTCACAGATCCACTCATTTGTTGACAAGCCTGGTATGCTTCCGGTGTATAATGCCAGAAGGCCTACCTCTAGTAGATCATATTTCCAAGGATTGATGGTTAGTGAGAAGTTCATTTATTCTCCACAAAAGCGGGAGGGAGTCCTAATATCATGTGTGAAGACAACAGAAGCTGCCAAGACTGAGAATTCCAGTGTTTTGTCAGACAGTAAGCCAGAAAGCACATCAGAGGGATCTCCTCAAAGCACTGTGTTTCCTAGTGGATATGAGGCTCTGATGCTAGAAGTCTGTGACGAGACAGAGATTGCTGAACTGAAACTTAAGATTGGAGACTTTGAAATGCATTTGAAGCGGAACATTGGAGCCACCAATGCTCCAGTGTCTACCATCTCGCCAACAGTAGCACCACCTATTCCATCTCCACCCATGGTTGAATCAGCTCCTGCTCCCCCTCCAGCATCTGCACCAAAACCTTCTCCTGCCAAAACTACTCCATTTAATAATGGCGCTGTATCCAAGTCCCCAAAATTAGCAGCATTGGAGGCTTCTGGATCTAATGGATATGTTCTGGTTACTTCTCCAACGGTTGGTTCGTTTCGAAGGGGCAGAACAGTGAAAGGAAAGAAGCAACCTCCTATCTTTCAAGAGGGTGATCTTATCAAAGAAGGACAGGTAATTGGGTACGTGGATCAGTTTGGCACTGAACTTCCTGTGAAGACTGATGTGGGTGGAGAAGTTTTGAAGCTCCTATTCAATGACGGAGATGCCGTTGGTTTTGGAGACGCACTTATTGCCGTCTTGCCATCATTCCCCGGAATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

290

Amino Acids

30.68

Weight (kDa)

6.2

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 635
AccI GTMKAC 1 cut(s) 431
AciI CCGC 2 cut(s) 181, 400
AclWI GGATC 5 cut(s) 18, 52, 289, 607, 744
AcsI RAATTY 1 cut(s) 235
AfaI GTAC 1 cut(s) 731
AfiI CCNNNNNNNGG 1 cut(s) 635
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 7 cut(s) 31, 386, 397, 482, 692, 790, 802
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 2 cut(s) 622, 654
AluBI AGCT 3 cut(s) 221, 488, 793
AluI AGCT 3 cut(s) 221, 488, 793
Alw26I GTCTC 3 cut(s) 338, 372, 822
AlwI GGATC 5 cut(s) 18, 52, 289, 607, 744
AlwNI CAGNNNCTG 3 cut(s) 221, 491, 509
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 221, 581
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 1 cut(s) 557
AsuC2I CCSGG 1 cut(s) 861
AsuHPI GGTGA 1 cut(s) 710
AsuII TTCGAA 1 cut(s) 646
BbsI GAAGAC 2 cut(s) 215, 770
BbvI GCAGC 2 cut(s) 208, 593
BccI CCATC 4 cut(s) 139, 443, 472, 859
BceAI ACGGC 2 cut(s) 800, 827
BciT130I CCWGG 1 cut(s) 81
BciVI GTATCC 1 cut(s) 571
BcnI CCSGG 1 cut(s) 861
BcoDI GTCTC 3 cut(s) 338, 372, 822
BfaI CTAG 3 cut(s) 120, 306, 329
BfoI RGCGCY 1 cut(s) 558
BfrI CTTAAG 1 cut(s) 368
BfuI GTATCC 1 cut(s) 571
BisI GCNGC 2 cut(s) 222, 582
BlsI GCNGC 2 cut(s) 223, 583
Bme1390I CCNGG 2 cut(s) 81, 861
BmiI GGNNCC 1 cut(s) 412
BmrFI CCNGG 2 cut(s) 81, 861
BmsI GCATC 3 cut(s) 315, 515, 802
BpiI GAAGAC 2 cut(s) 215, 770
BpmI CTGGAG 2 cut(s) 408, 486
Bpu14I TTCGAA 1 cut(s) 646
BpuMI CCSGG 1 cut(s) 861
BsaAI YACGTR 1 cut(s) 733
BsaJI CCNNGG 3 cut(s) 136, 474, 859
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 801, 831
Bsc4I CCNNNNNNNGG 1 cut(s) 635
Bse1I ACTGG 3 cut(s) 240, 425, 868
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 3 cut(s) 136, 474, 859
BseLI CCNNNNNNNGG 1 cut(s) 635
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 240, 425, 868
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 2 cut(s) 208, 593
BsgI GTGCAG 1 cut(s) 495
BshFI GGCC 1 cut(s) 112
BsiSI CCGG 2 cut(s) 92, 861
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 1 cut(s) 635
BsmAI GTCTC 3 cut(s) 338, 372, 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 646
Bsp143I GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 181, 400
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 1 cut(s) 412
BspPI GGATC 5 cut(s) 18, 52, 289, 607, 744
BspT104I TTCGAA 1 cut(s) 646
BspTI CTTAAG 1 cut(s) 368
BsrI ACTGG 3 cut(s) 240, 425, 868
BssECI CCNNGG 3 cut(s) 136, 474, 859
BssMI GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
BssT1I CCWWGG 2 cut(s) 136, 474
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 7 cut(s) 35, 49, 257, 298, 448, 634, 661
BstAFI CTTAAG 1 cut(s) 368
BstAPI GCANNNNNTGC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 474
BstH2I RGCGCY 1 cut(s) 558
BstHHI GCGC 1 cut(s) 557
BstKTI GATC 7 cut(s) 26, 60, 128, 284, 602, 703, 739
BstMAI GTCTC 3 cut(s) 338, 372, 822
BstMBI GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
BstMWI GCNNNNNNNGC 5 cut(s) 85, 397, 419, 590, 839
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 2 cut(s) 79, 859
BstV1I GCAGC 2 cut(s) 208, 593
BstV2I GAAGAC 2 cut(s) 215, 770
BstX2I RGATCY 3 cut(s) 57, 281, 599
BstYI RGATCY 3 cut(s) 57, 281, 599
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 474
BtsIMutI CAGTG 5 cut(s) 247, 294, 432, 666, 747
CaiI CAGNNNCTG 3 cut(s) 221, 491, 509
CfoI GCGC 1 cut(s) 557
CseI GACGC 1 cut(s) 839
Csp6I GTAC 1 cut(s) 730
CviAII CATG 2 cut(s) 201, 475
CviJI RGCY 9 cut(s) 79, 112, 221, 262, 320, 413, 488, 593, 793
CviKI_1 RGCY 9 cut(s) 79, 112, 221, 262, 320, 413, 488, 593, 793
CviQI GTAC 1 cut(s) 730
DdeI CTNAG 1 cut(s) 231
DpnI GATC 7 cut(s) 25, 59, 127, 283, 601, 702, 738
DpnII GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
Eco130I CCWWGG 2 cut(s) 136, 474
Eco147I AGGCCT 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 136, 474
EcoT22I ATGCAT 1 cut(s) 393
ErhI CCWWGG 2 cut(s) 136, 474
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 2 cut(s) 204, 478
FaiI YATR 7 cut(s) 86, 99, 130, 202, 315, 476, 612
FaqI GGGAC 1 cut(s) 553
FatI CATG 2 cut(s) 200, 474
FauI CCCGC 1 cut(s) 174
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 2 cut(s) 222, 582
Fsp4HI GCNGC 2 cut(s) 222, 582
FspBI CTAG 3 cut(s) 120, 306, 329
GlaI GCGC 1 cut(s) 556
GluI GCNGC 2 cut(s) 222, 582
GsuI CTGGAG 2 cut(s) 408, 486
HaeII RGCGCY 1 cut(s) 558
HaeIII GGCC 1 cut(s) 112
HapII CCGG 2 cut(s) 92, 861
HgaI GACGC 1 cut(s) 839
HhaI GCGC 1 cut(s) 557
Hin1II CATG 2 cut(s) 204, 478
Hin6I GCGC 1 cut(s) 555
HinP1I GCGC 1 cut(s) 555
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 4 cut(s) 5, 189, 482, 864
HpaII CCGG 2 cut(s) 92, 861
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 2 cut(s) 73, 432
Hpy188I TCNGA 4 cut(s) 23, 253, 277, 324
Hpy188III TCNNGA 2 cut(s) 597, 692
Hpy8I GTNNAC 2 cut(s) 73, 432
HpyAV CCTTC 5 cut(s) 37, 102, 531, 642, 707
HpyCH4III ACNGT 7 cut(s) 35, 49, 257, 298, 448, 634, 661
HpyCH4IV ACGT 1 cut(s) 732
HpyCH4V TGCA 2 cut(s) 391, 512
HpyF10VI GCNNNNNNNGC 5 cut(s) 85, 397, 419, 590, 839
HpyF3I CTNAG 1 cut(s) 231
HpySE526I ACGT 1 cut(s) 732
Hsp92II CATG 2 cut(s) 204, 478
HspAI GCGC 1 cut(s) 555
Kzo9I GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
LmnI GCTCC 5 cut(s) 410, 427, 493, 499, 798
Lsp1109I GCAGC 2 cut(s) 208, 593
LweI GCATC 3 cut(s) 315, 515, 802
MaeI CTAG 3 cut(s) 120, 306, 329
MaeII ACGT 1 cut(s) 732
MaeIII GTNAC 2 cut(s) 338, 619
MalI GATC 7 cut(s) 25, 59, 127, 283, 601, 702, 738
MboI GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
MboII GAAGA 2 cut(s) 220, 775
MflI RGATCY 3 cut(s) 57, 281, 599
MluCI AATT 3 cut(s) 235, 575, 723
MlyI GAGTC 2 cut(s) 14, 198
MmeI TCCRAC 1 cut(s) 653
Mph1103I ATGCAT 1 cut(s) 393
MseI TTAA 2 cut(s) 369, 546
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 92, 861
MspR9I CCNGG 2 cut(s) 81, 861
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 5 cut(s) 85, 397, 419, 590, 839
NciI CCSGG 1 cut(s) 861
NcoI CCATGG 1 cut(s) 474
NdeII GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
NlaIII CATG 2 cut(s) 204, 478
NlaIV GGNNCC 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 338
NsiI ATGCAT 1 cut(s) 393
NspV TTCGAA 1 cut(s) 646
PceI AGGCCT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 2 cut(s) 638, 813
PfeI GAWTC 2 cut(s) 482, 864
PflMI CCANNNNNTGG 1 cut(s) 635
PkrI GCNGC 2 cut(s) 223, 583
PleI GAGTC 2 cut(s) 13, 197
PpsI GAGTC 2 cut(s) 13, 197
Ppu21I YACGTR 1 cut(s) 733
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 412
PstNI CAGNNNCTG 3 cut(s) 221, 491, 509
PsuI RGATCY 3 cut(s) 57, 281, 599
RsaI GTAC 1 cut(s) 731
RsaNI GTAC 1 cut(s) 730
SaqAI TTAA 2 cut(s) 369, 546
SatI GCNGC 2 cut(s) 222, 582
Sau3AI GATC 7 cut(s) 23, 57, 125, 281, 599, 700, 736
SchI GAGTC 2 cut(s) 14, 198
ScrFI CCNGG 2 cut(s) 81, 861
SetI ASST 9 cut(s) 119, 223, 460, 490, 523, 682, 723, 735, 795
SfaNI GCATC 3 cut(s) 315, 515, 802
SfuI TTCGAA 1 cut(s) 646
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 3 cut(s) 235, 575, 723
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 2 cut(s) 181, 400
SspMI CTAG 3 cut(s) 120, 306, 329
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 79, 859
StyI CCWWGG 2 cut(s) 136, 474
TaaI ACNGT 7 cut(s) 35, 49, 257, 298, 448, 634, 661
TaiI ACGT 1 cut(s) 735
TaqI TCGA 1 cut(s) 646
TasI AATT 3 cut(s) 235, 575, 723
TfiI GAWTC 2 cut(s) 482, 864
Tru1I TTAA 2 cut(s) 369, 546
Tru9I TTAA 2 cut(s) 369, 546
TscAI CASTG 5 cut(s) 247, 301, 432, 666, 754
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 2 cut(s) 221, 581
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 1 cut(s) 151
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 5 cut(s) 247, 301, 432, 666, 754
Van91I CCANNNNNTGG 1 cut(s) 635
Vha464I CTTAAG 1 cut(s) 368
XapI RAATTY 1 cut(s) 235
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 3 cut(s) 120, 306, 329
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.