Rorug01G0362900

Biotin carboxyl carrier protein of acetyl-CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006727
Physical Location & Seq
Reverse (-)
47827384 .. 47830622
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0362900.1

Sequence Viewer

Length: 1266 bp
ATGGGCATTTCGTGCGACGACGTCGTTATAGTCCAGCAGGGGAAGAATCCCGGCGAGCCGACCGTCGTGACGGTCAATTGTCCCGACAAGCCCGGCCTCGGCTGCGACCTCTGCAGAACCGTGCTTCAATTCGGGCTTTGCATTACCAAAGGAGATTTCACGACGGACGGGAAGTGGTGCTACATAGTGCTGTGGGTGGTGGCGAATCAGAGCTCAATGAAGGTGGATTGGGAGAGGTTGAAGGAGCGGCTGCTCTCTGCTTGCCCGTCTTCGTGTTTCTATAAGGTTTACCTCAATATTCAGTCCAGTGCTCCCTCGCCGTCGCCGGTTTACCTTTTGAAGTTTTGGTGCTTTAACCAGAGAGGCTCACTGCATGATGTTACTAAGGTGCTGTGTGAGCTTGAGATGCTGATTCAGAGAGTGAAAGTGATGCCAACTCCGGATGGGAGGGTTTTGGACCTGTTCTTCATCACGGATTGCTTAGATTTGCTACACACGAAAAAGAGGAGAGATGACATATGTGAGCATCTGATGGCTGCCCTAGGAGAGTACTGTATCACCTGTGAGGTTCAGTTGGCAGGGCCTGAATATGAAAGTTTGCAGGGAAATTATTCTCTTCCATCAGCAATTGCCGAAGAATTATTTGGCTATCAGCTAACAGATGACAAAGCTTGTCCGAAAGCTCTCAGCCCAAGTTTGGCAACAGTGAAAAACACAAGTATTACGTTGGATAATACACTGAGCCCTGTTCATACGTTGCTGCAAATACAATGTGTGGATCAAAAGGGCCTCTTTTATGACGTACTAAGGACTTCCAAGGATTGCAAAATTCAGATTGCCTATGGTAGATTCTCTTCTACTGTCAAAGGCATCCGAAATTTGGAACTGTTTATTCAGCACACAGATGGGAAAAAGATTGTAGATCCTGAAAGACAGGTTGCATTGTGTTCTCGGTTGAAGGAGGAGATGCTTCACCCGCTGAGAGTGATCATTGCCAACAAGGGTCCAGATACTGAACTCCTAGTCGCTAATCCAGTTGAGTTATCTGGAAAAGGACGGCCCCTCGTCTTTTATGATGTTACTCTTGCACTGAAAAAGTTGGGAATATCTATTTTCTTGGCTGAAATTGTAAGGCATGAGACTGAAGATTGCCAGTGGGAAGTCTATAGATTTCTTTTGGATGATAGTCGAGAATTTCCTTTGGGGAGCAGAGAAGCTAGAAATAAGATTGTACAAAGAGTTAGAAGAACATTGATGGGATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

421

Amino Acids

47.52

Weight (kDa)

6.66

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACR10_N PF24914 12 - 89 1.1e-40 ACT domain-containing protein ACR10, N-terminal
ACT_ACR9_3rd PF24931 240 - 337 2.2e-46 ACR9-like, ACT-like domain
ACT_ACR9_C PF24926 338 - 420 6.6e-38 ACT domain-containing protein ACR9, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccBSI CCGCTC 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 439
AciI CCGC 2 cut(s) 247, 977
AclWI GGATC 2 cut(s) 786, 917
AcsI RAATTY 3 cut(s) 828, 877, 1193
AcuI CTGAAG 1 cut(s) 1164
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 3 cut(s) 551, 804, 1233
AfiI CCNNNNNNNGG 3 cut(s) 98, 697, 880
AgsI TTSAA 4 cut(s) 128, 241, 340, 958
AjuI GAANNNNNNNTTGG 4 cut(s) 140, 172, 627, 659
AluBI AGCT 6 cut(s) 213, 400, 655, 671, 683, 1217
AluI AGCT 6 cut(s) 213, 400, 655, 671, 683, 1217
Alw21I GWGCWC 2 cut(s) 215, 313
Alw26I GTCTC 1 cut(s) 1133
AlwI GGATC 2 cut(s) 786, 917
AlwNI CAGNNNCTG 2 cut(s) 584, 1013
Aor13HI TCCGGA 1 cut(s) 439
AoxI GGCC 4 cut(s) 94, 581, 787, 1058
ApeKI GCWGC 4 cut(s) 102, 250, 536, 760
ApoI RAATTY 3 cut(s) 828, 877, 1193
Asp700I GAANNNNTTC 1 cut(s) 610
AspA2I CCTAGG 1 cut(s) 541
AspS9I GGNCC 5 cut(s) 457, 581, 787, 1004, 1059
AsuC2I CCSGG 2 cut(s) 51, 93
AsuHPI GGTGA 2 cut(s) 550, 965
AvaII GGWCC 2 cut(s) 457, 1004
AvrII CCTAGG 1 cut(s) 541
BanII GRGCYC 2 cut(s) 215, 746
BarI GAAGNNNNNNTAC 4 cut(s) 164, 196, 838, 870
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 2 cut(s) 215, 313
BbvI GCAGC 4 cut(s) 89, 237, 523, 747
BccI CCATC 6 cut(s) 437, 526, 628, 899, 1249, 1254
BceAI ACGGC 2 cut(s) 304, 1073
BclI TGATCA 1 cut(s) 987
BcnI CCSGG 2 cut(s) 51, 93
BcoDI GTCTC 1 cut(s) 1133
BfaI CTAG 3 cut(s) 542, 1022, 1218
BfmI CTRYAG 2 cut(s) 112, 1165
BisI GCNGC 5 cut(s) 103, 248, 251, 537, 761
BlnI CCTAGG 1 cut(s) 541
BlsI GCNGC 5 cut(s) 104, 249, 252, 538, 762
BmcAI AGTACT 1 cut(s) 551
Bme1390I CCNGG 2 cut(s) 51, 93
Bme18I GGWCC 2 cut(s) 457, 1004
BmgT120I GGNCC 5 cut(s) 457, 581, 787, 1004, 1059
BmiI GGNNCC 2 cut(s) 1005, 1061
BmrFI CCNGG 2 cut(s) 51, 93
BmsI GCATC 5 cut(s) 396, 420, 535, 879, 957
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 2 cut(s) 51, 93
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 97, 541, 816
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 697, 880
Bse118I RCCGGY 1 cut(s) 325
Bse1I ACTGG 3 cut(s) 306, 1034, 1153
Bse3DI GCAATG 1 cut(s) 990
BseAI TCCGGA 1 cut(s) 439
BseDI CCNNGG 3 cut(s) 97, 541, 816
BseGI GGATG 4 cut(s) 448, 870, 1186, 1265
BseLI CCNNNNNNNGG 3 cut(s) 98, 697, 880
BseMI GCAATG 1 cut(s) 990
BseMII CTCAG 3 cut(s) 700, 731, 971
BseNI ACTGG 3 cut(s) 306, 1034, 1153
BseRI GAGGAG 2 cut(s) 520, 977
BseXI GCAGC 4 cut(s) 89, 237, 523, 747
Bsh1285I CGRYCG 1 cut(s) 63
BshFI GGCC 4 cut(s) 96, 583, 789, 1060
BsiEI CGRYCG 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 215, 313
BsiSI CCGG 4 cut(s) 51, 93, 326, 440
BslFI GGGAC 1 cut(s) 66
BslI CCNNNNNNNGG 3 cut(s) 98, 697, 880
BsmAI GTCTC 1 cut(s) 1133
BsmFI GGGAC 1 cut(s) 66
BsnI GGCC 4 cut(s) 96, 583, 789, 1060
Bsp1286I GDGCHC 3 cut(s) 215, 313, 746
Bsp13I TCCGGA 1 cut(s) 439
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 3 cut(s) 778, 922, 987
BspACI CCGC 2 cut(s) 247, 977
BspANI GGCC 4 cut(s) 96, 583, 789, 1060
BspCNI CTCAG 3 cut(s) 699, 732, 972
BspEI TCCGGA 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 1005, 1061
BspMAI CTGCAG 1 cut(s) 116
BspPI GGATC 2 cut(s) 786, 917
BsrBI CCGCTC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 990
BsrFI RCCGGY 1 cut(s) 325
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 3 cut(s) 306, 1034, 1153
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 3 cut(s) 97, 541, 816
BssMI GATC 3 cut(s) 778, 922, 987
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 2 cut(s) 541, 816
Bst4CI ACNGT 7 cut(s) 64, 73, 121, 554, 706, 862, 888
Bst6I CTCTTC 2 cut(s) 621, 859
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 1231
BstC8I GCNNGC 2 cut(s) 56, 262
BstDEI CTNAG 6 cut(s) 384, 481, 686, 740, 806, 980
BstF5I GGATG 4 cut(s) 448, 870, 1186, 1265
BstKTI GATC 3 cut(s) 781, 925, 990
BstMAI GTCTC 1 cut(s) 1133
BstMBI GATC 3 cut(s) 778, 922, 987
BstMCI CGRYCG 1 cut(s) 63
BstMWI GCNNNNNNNGC 6 cut(s) 12, 102, 111, 397, 406, 976
BstSCI CCNGG 2 cut(s) 49, 91
BstSFI CTRYAG 2 cut(s) 112, 1165
BstV1I GCAGC 4 cut(s) 89, 237, 523, 747
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 922
BstYI RGATCY 1 cut(s) 922
BsuRI GGCC 4 cut(s) 96, 583, 789, 1060
BtsCI GGATG 4 cut(s) 448, 870, 1186, 1265
BtsI GCAGTG 1 cut(s) 368
BtsIMutI CAGTG 6 cut(s) 313, 368, 711, 737, 1088, 1160
Cac8I GCNNGC 2 cut(s) 56, 262
CaiI CAGNNNCTG 2 cut(s) 584, 1013
Cfr10I RCCGGY 1 cut(s) 325
Cfr13I GGNCC 5 cut(s) 457, 581, 787, 1004, 1059
Csp6I GTAC 3 cut(s) 550, 803, 1232
CspCI CAANNNNNGTGG 2 cut(s) 204, 239
CviAII CATG 2 cut(s) 374, 1136
CviQI GTAC 3 cut(s) 550, 803, 1232
DdeI CTNAG 6 cut(s) 384, 481, 686, 740, 806, 980
DpnI GATC 3 cut(s) 780, 924, 989
DpnII GATC 3 cut(s) 778, 922, 987
Eam1104I CTCTTC 2 cut(s) 621, 859
EarI CTCTTC 2 cut(s) 621, 859
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 2 cut(s) 541, 816
Eco24I GRGCYC 2 cut(s) 215, 746
Eco47I GGWCC 2 cut(s) 457, 1004
Eco53kI GAGCTC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 1164
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 581, 787
EcoT14I CCWWGG 2 cut(s) 541, 816
EcoT38I GRGCYC 2 cut(s) 215, 746
ErhI CCWWGG 2 cut(s) 541, 816
FaeI CATG 2 cut(s) 377, 1139
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FaqI GGGAC 1 cut(s) 66
FatI CATG 2 cut(s) 373, 1135
FauI CCCGC 1 cut(s) 984
FauNDI CATATG 1 cut(s) 518
FbaI TGATCA 1 cut(s) 987
Fnu4HI GCNGC 5 cut(s) 103, 248, 251, 537, 761
FokI GGATG 3 cut(s) 455, 857, 1193
FriOI GRGCYC 2 cut(s) 215, 746
Fsp4HI GCNGC 5 cut(s) 103, 248, 251, 537, 761
FspBI CTAG 3 cut(s) 542, 1022, 1218
GluI GCNGC 5 cut(s) 103, 248, 251, 537, 761
HaeIII GGCC 4 cut(s) 96, 583, 789, 1060
HapII CCGG 4 cut(s) 51, 93, 326, 440
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 2 cut(s) 377, 1139
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 4 cut(s) 46, 205, 412, 849
HpaII CCGG 4 cut(s) 51, 93, 326, 440
HphI GGTGA 2 cut(s) 550, 965
Hpy166II GTNNAC 2 cut(s) 289, 331
Hpy188I TCNGA 6 cut(s) 210, 417, 531, 678, 834, 875
Hpy188III TCNNGA 8 cut(s) 67, 83, 160, 440, 926, 1007, 1047, 1190
Hpy8I GTNNAC 2 cut(s) 289, 331
Hpy99I CGWCG 6 cut(s) 20, 23, 26, 68, 166, 325
HpyAV CCTTC 3 cut(s) 214, 235, 952
HpyCH4III ACNGT 7 cut(s) 64, 73, 121, 554, 706, 862, 888
HpyCH4IV ACGT 4 cut(s) 21, 725, 755, 801
HpyCH4V TGCA 8 cut(s) 114, 141, 373, 601, 763, 825, 941, 1088
HpyF10VI GCNNNNNNNGC 6 cut(s) 12, 102, 111, 397, 406, 976
HpyF3I CTNAG 6 cut(s) 384, 481, 686, 740, 806, 980
HpySE526I ACGT 4 cut(s) 21, 725, 755, 801
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 2 cut(s) 377, 1139
Kpn2I TCCGGA 1 cut(s) 439
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 3 cut(s) 778, 922, 987
LmnI GCTCC 3 cut(s) 244, 316, 1206
Lsp1109I GCAGC 4 cut(s) 89, 237, 523, 747
LweI GCATC 5 cut(s) 396, 420, 535, 879, 957
MaeI CTAG 3 cut(s) 542, 1022, 1218
MaeII ACGT 4 cut(s) 21, 725, 755, 801
MaeIII GTNAC 3 cut(s) 67, 379, 1078
MalI GATC 3 cut(s) 780, 924, 989
MbiI CCGCTC 1 cut(s) 247
MboI GATC 3 cut(s) 778, 922, 987
MboII GAAGA 8 cut(s) 55, 261, 457, 608, 647, 846, 1157, 1257
MfeI CAATTG 2 cut(s) 76, 627
MflI RGATCY 1 cut(s) 922
MhlI GDGCHC 3 cut(s) 215, 313, 746
MluCI AATT 9 cut(s) 76, 128, 607, 627, 638, 828, 877, 1125, 1193
MmeI TCCRAC 1 cut(s) 708
MroI TCCGGA 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 903
MspA1I CMGCKG 1 cut(s) 979
MspI CCGG 4 cut(s) 51, 93, 326, 440
MspR9I CCNGG 2 cut(s) 51, 93
MunI CAATTG 2 cut(s) 76, 627
MwoI GCNNNNNNNGC 6 cut(s) 12, 102, 111, 397, 406, 976
NciI CCSGG 2 cut(s) 51, 93
NdeI CATATG 1 cut(s) 518
NdeII GATC 3 cut(s) 778, 922, 987
NlaIII CATG 2 cut(s) 377, 1139
NlaIV GGNNCC 2 cut(s) 1005, 1061
NmeAIII GCCGAG 1 cut(s) 78
NmuCI GTSAC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 4 cut(s) 46, 205, 412, 849
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 5 cut(s) 104, 249, 252, 538, 762
Psp124BI GAGCTC 1 cut(s) 215
PspN4I GGNNCC 2 cut(s) 1005, 1061
PspPI GGNCC 5 cut(s) 457, 581, 787, 1004, 1059
PstI CTGCAG 1 cut(s) 116
PstNI CAGNNNCTG 2 cut(s) 584, 1013
PsuI RGATCY 1 cut(s) 922
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 3 cut(s) 551, 804, 1233
RsaNI GTAC 3 cut(s) 550, 803, 1232
RseI CAYNNNNRTG 1 cut(s) 903
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 5 cut(s) 103, 248, 251, 537, 761
Sau3AI GATC 3 cut(s) 778, 922, 987
Sau96I GGNCC 5 cut(s) 457, 581, 787, 1004, 1059
ScaI AGTACT 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 51, 93
SduI GDGCHC 3 cut(s) 215, 313, 746
SfaNI GCATC 5 cut(s) 396, 420, 535, 879, 957
SfcI CTRYAG 2 cut(s) 112, 1165
SinI GGWCC 2 cut(s) 457, 1004
SmiMI CAYNNNNRTG 1 cut(s) 903
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
Sse9I AATT 9 cut(s) 76, 128, 607, 627, 638, 828, 877, 1125, 1193
SsiI CCGC 2 cut(s) 247, 977
SspI AATATT 1 cut(s) 298
SspMI CTAG 3 cut(s) 542, 1022, 1218
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 49, 91
StyI CCWWGG 2 cut(s) 541, 816
TaaI ACNGT 7 cut(s) 64, 73, 121, 554, 706, 862, 888
TaiI ACGT 4 cut(s) 24, 728, 758, 804
TaqI TCGA 1 cut(s) 1189
TasI AATT 9 cut(s) 76, 128, 607, 627, 638, 828, 877, 1125, 1193
TatI WGTACW 2 cut(s) 549, 1231
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 4 cut(s) 46, 205, 412, 849
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 6 cut(s) 313, 375, 711, 744, 1095, 1160
TseFI GTSAC 1 cut(s) 67
TseI GCWGC 4 cut(s) 102, 250, 536, 760
Tsp45I GTSAC 1 cut(s) 67
TspDTI ATGAA 4 cut(s) 233, 457, 606, 740
TspGWI ACGGA 2 cut(s) 179, 488
TspRI CASTG 6 cut(s) 313, 375, 711, 744, 1095, 1160
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 2 cut(s) 457, 1004
XapI RAATTY 3 cut(s) 828, 877, 1193
XmaJI CCTAGG 1 cut(s) 541
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 3 cut(s) 542, 1022, 1218
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.