pycom01g17630

Biotin carboxyl carrier protein of acetyl-CoA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0006727
Physical Location & Seq
Forward (+)
16843465 .. 16847169
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17630.1

Sequence Viewer

Length: 867 bp
ATGGAGTCCTCCGCCGCTCTCCGATCCTTCAACTGTTCTGTAGGCACAGTTTCCCATGTCCGGTCCTTGCTTGACAAGCCTGGTATGTTGCCTGTGCACAATACCAGAAGGCCCGTGCCAAGTAGGTCATATTTCCAAGGCTCGTTGGTTAGTGAGAAGTTCATTTATTCTTCACAAAAGCGAAAGGGAGTGCTAGTATCATGTGTGAAGACATCTGACGCTACCAAGAGTGAAAATTCCAGCGTTTCAGCAGATAGTAAACCACAAGGCCCATCAGAAAAAGCTACCCAACCTGCAATTTTTCCTAGAGGATTTGAGGCACTGATGCTGGAAGTTTGTGATGAGACTGAGGTTGCTGAACTGAAACTTAAGGTTGGAGACTTTGAAATGCATTTGAAGCGGAACATTGCAGGCACCAGTGCCCCAGTGCCTAGCATCTCGCCAGCAACACCACCACCTGTTCCATCTGCACCTATGGATTCAACTCCTGCTCCCCCTCCAGCATCACCACCAAAATCTTCTGAAAAAACCACTCCATTTACAAACGTATCTGCAGACAAGTCATCAAGATTAGCAGCCTTGGAGGCTTCTGGTGCTAATGGATATGGTCTAGTTTCTTCTCCAACGGTTGGTTCATTCCGCAGGGGAAGAACTGTGAAAGGAAAGAAGCAACCTCCTATCTGTAAAGAGGGTGATTTGATCGATCAAGGACAGGTAGTCGGATATGTGGATCAGTTTGGCACCGAACTTCCTGTGAAGACTGATTTGGGTGGAGAAGTTTTGAAGCTCCTCTTCAACGACGGAGAAGCTGTTGGTTTCGGAGACCCCCTTATCGCTGTCTTGCCCTCATTTCCCGGTATCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Biotin_lipoyl

Protein Analysis

289

Amino Acids

30.37

Weight (kDa)

8.59

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 2 cut(s) 413, 740
AccB7I CCANNNNNTGG 1 cut(s) 629
AccBSI CCGCTC 1 cut(s) 17
AciI CCGC 4 cut(s) 12, 15, 400, 640
AclWI GGATC 2 cut(s) 18, 738
AcsI RAATTY 1 cut(s) 235
AfiI CCNNNNNNNGG 2 cut(s) 60, 629
AflII CTTAAG 1 cut(s) 368
AgsI TTSAA 6 cut(s) 31, 386, 397, 483, 784, 796
AhdI GACNNNNNGTC 1 cut(s) 716
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 4 cut(s) 616, 648, 749, 781
AluBI AGCT 3 cut(s) 284, 787, 809
AluI AGCT 3 cut(s) 284, 787, 809
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 338, 372, 816
Alw44I GTGCAC 1 cut(s) 95
AlwI GGATC 2 cut(s) 18, 738
AoxI GGCC 2 cut(s) 110, 268
ApaLI GTGCAC 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 235
AspS9I GGNCC 3 cut(s) 63, 111, 269
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 2 cut(s) 498, 704
AvaII GGWCC 1 cut(s) 63
BaeGI GKGCMC 2 cut(s) 99, 424
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BanI GGYRCC 2 cut(s) 413, 740
BbsI GAAGAC 2 cut(s) 215, 764
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 587
BccI CCATC 2 cut(s) 280, 472
BciT130I CCWGG 1 cut(s) 81
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 3 cut(s) 338, 372, 816
BfaI CTAG 4 cut(s) 194, 306, 432, 611
BfmI CTRYAG 2 cut(s) 39, 552
BfrI CTTAAG 1 cut(s) 368
BfuAI ACCTGC 1 cut(s) 301
BglI GCCNNNNNGGC 1 cut(s) 584
BisI GCNGC 2 cut(s) 15, 576
BlsI GCNGC 2 cut(s) 16, 577
Bme1390I CCNGG 2 cut(s) 81, 855
Bme18I GGWCC 1 cut(s) 63
BmeRI GACNNNNNGTC 1 cut(s) 716
BmgT120I GGNCC 3 cut(s) 63, 111, 269
BmiI GGNNCC 2 cut(s) 415, 742
BmrFI CCNGG 2 cut(s) 81, 855
BmrI ACTGGG 1 cut(s) 419
BmsI GCATC 3 cut(s) 315, 444, 512
BmuI ACTGGG 1 cut(s) 419
BpiI GAAGAC 2 cut(s) 215, 764
BpmI CTGGAG 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 855
Bsa29I ATCGAT 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 2 cut(s) 136, 579
BsaWI WCCGGW 1 cut(s) 60
BsaXI ACNNNNNCTCC 6 cut(s) 180, 210, 475, 505, 795, 825
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 629
Bse1I ACTGG 3 cut(s) 417, 425, 862
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 81
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 2 cut(s) 136, 579
BseLI CCNNNNNNNGG 2 cut(s) 60, 629
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 3 cut(s) 417, 425, 862
BseRI GAGGAG 1 cut(s) 779
BseSI GKGCMC 2 cut(s) 99, 424
BseXI GCAGC 1 cut(s) 587
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 112, 270
BshNI GGYRCC 2 cut(s) 413, 740
BshVI ATCGAT 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 61, 855
BslI CCNNNNNNNGG 2 cut(s) 60, 629
BsmAI GTCTC 3 cut(s) 338, 372, 816
BsnI GGCC 2 cut(s) 112, 270
Bso31I GGTCTC 1 cut(s) 816
Bsp1286I GDGCHC 2 cut(s) 99, 424
Bsp143I GATC 4 cut(s) 23, 699, 703, 730
BspACI CCGC 4 cut(s) 12, 15, 400, 640
BspANI GGCC 2 cut(s) 112, 270
BspCNI CTCAG 1 cut(s) 340
BspDI ATCGAT 1 cut(s) 702
BspLI GGNNCC 2 cut(s) 415, 742
BspMAI CTGCAG 1 cut(s) 556
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 2 cut(s) 18, 738
BspT107I GGYRCC 2 cut(s) 413, 740
BspTI CTTAAG 1 cut(s) 368
BspTNI GGTCTC 1 cut(s) 816
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 3 cut(s) 417, 425, 862
BssECI CCNNGG 2 cut(s) 136, 579
BssMI GATC 4 cut(s) 23, 699, 703, 730
BssT1I CCWWGG 2 cut(s) 136, 579
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 35, 49, 628, 655
Bst6I CTCTTC 1 cut(s) 797
BstAFI CTTAAG 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 412, 444
BstDEI CTNAG 1 cut(s) 348
BstKTI GATC 4 cut(s) 26, 702, 706, 733
BstMAI GTCTC 3 cut(s) 338, 372, 816
BstMBI GATC 4 cut(s) 23, 699, 703, 730
BstMWI GCNNNNNNNGC 4 cut(s) 76, 397, 584, 593
BstNI CCWGG 1 cut(s) 81
BstSCI CCNGG 2 cut(s) 79, 853
BstSFI CTRYAG 2 cut(s) 39, 552
BstSLI GKGCMC 2 cut(s) 99, 424
BstV1I GCAGC 1 cut(s) 587
BstV2I GAAGAC 2 cut(s) 215, 764
Bsu15I ATCGAT 1 cut(s) 702
BsuRI GGCC 2 cut(s) 112, 270
BsuTUI ATCGAT 1 cut(s) 702
BtsIMutI CAGTG 3 cut(s) 320, 424, 432
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 2 cut(s) 412, 444
Cfr13I GGNCC 3 cut(s) 63, 111, 269
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 1 cut(s) 227
CviAII CATG 2 cut(s) 56, 201
CviJI RGCY 9 cut(s) 79, 112, 141, 270, 284, 578, 587, 787, 809
CviKI_1 RGCY 9 cut(s) 79, 112, 141, 270, 284, 578, 587, 787, 809
DdeI CTNAG 1 cut(s) 348
DpnI GATC 4 cut(s) 25, 701, 705, 732
DpnII GATC 4 cut(s) 23, 699, 703, 730
DriI GACNNNNNGTC 1 cut(s) 716
Eam1104I CTCTTC 1 cut(s) 797
Eam1105I GACNNNNNGTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 797
Eco130I CCWWGG 2 cut(s) 136, 579
Eco31I GGTCTC 1 cut(s) 816
Eco47I GGWCC 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 2 cut(s) 136, 579
EcoT22I ATGCAT 1 cut(s) 393
ErhI CCWWGG 2 cut(s) 136, 579
FaeI CATG 2 cut(s) 59, 204
FaiI YATR 7 cut(s) 57, 86, 130, 202, 476, 606, 726
FalI AAGNNNNNCTT 2 cut(s) 776, 808
FatI CATG 2 cut(s) 55, 200
Fnu4HI GCNGC 2 cut(s) 15, 576
Fsp4HI GCNGC 2 cut(s) 15, 576
FspBI CTAG 4 cut(s) 194, 306, 432, 611
GluI GCNGC 2 cut(s) 15, 576
GsuI CTGGAG 1 cut(s) 483
HaeIII GGCC 2 cut(s) 112, 270
HapII CCGG 2 cut(s) 61, 855
HgaI GACGC 1 cut(s) 227
Hin1II CATG 2 cut(s) 59, 204
HinfI GANTC 2 cut(s) 5, 479
HpaII CCGG 2 cut(s) 61, 855
HphI GGTGA 2 cut(s) 498, 704
Hpy166II GTNNAC 2 cut(s) 97, 260
Hpy188I TCNGA 6 cut(s) 23, 217, 277, 523, 722, 821
Hpy188III TCNNGA 1 cut(s) 567
Hpy8I GTNNAC 2 cut(s) 97, 260
Hpy99I CGWCG 1 cut(s) 803
HpyAV CCTTC 2 cut(s) 37, 102
HpyCH4III ACNGT 4 cut(s) 35, 49, 628, 655
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 6 cut(s) 97, 296, 391, 410, 470, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 397, 584, 593
HpyF3I CTNAG 1 cut(s) 348
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 2 cut(s) 59, 204
Kzo9I GATC 4 cut(s) 23, 699, 703, 730
LmnI GCTCC 2 cut(s) 496, 792
Lsp1109I GCAGC 1 cut(s) 587
LweI GCATC 3 cut(s) 315, 444, 512
MaeI CTAG 4 cut(s) 194, 306, 432, 611
MaeII ACGT 1 cut(s) 546
MalI GATC 4 cut(s) 25, 701, 705, 732
MbiI CCGCTC 1 cut(s) 17
MboI GATC 4 cut(s) 23, 699, 703, 730
MboII GAAGA 7 cut(s) 162, 220, 510, 609, 660, 769, 784
MhlI GDGCHC 2 cut(s) 99, 424
MluCI AATT 2 cut(s) 235, 297
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 3 cut(s) 355, 647, 700
Mph1103I ATGCAT 1 cut(s) 393
MseI TTAA 1 cut(s) 369
MspCI CTTAAG 1 cut(s) 368
MspI CCGG 2 cut(s) 61, 855
MspR9I CCNGG 2 cut(s) 81, 855
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 4 cut(s) 76, 397, 584, 593
NciI CCSGG 1 cut(s) 855
NdeII GATC 4 cut(s) 23, 699, 703, 730
NlaIII CATG 2 cut(s) 59, 204
NlaIV GGNNCC 2 cut(s) 415, 742
NsiI ATGCAT 1 cut(s) 393
PfeI GAWTC 1 cut(s) 479
PflMI CCANNNNNTGG 1 cut(s) 629
PkrI GCNGC 2 cut(s) 16, 577
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 415, 742
PspPI GGNCC 3 cut(s) 63, 111, 269
PstI CTGCAG 1 cut(s) 556
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 15, 576
Sau3AI GATC 4 cut(s) 23, 699, 703, 730
Sau96I GGNCC 3 cut(s) 63, 111, 269
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 81, 855
SduI GDGCHC 2 cut(s) 99, 424
SfaNI GCATC 3 cut(s) 315, 444, 512
SfcI CTRYAG 2 cut(s) 39, 552
SinI GGWCC 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 2 cut(s) 235, 297
SsiI CCGC 4 cut(s) 12, 15, 400, 640
SspMI CTAG 4 cut(s) 194, 306, 432, 611
StyD4I CCNGG 2 cut(s) 79, 853
StyI CCWWGG 2 cut(s) 136, 579
TaaI ACNGT 4 cut(s) 35, 49, 628, 655
TaiI ACGT 1 cut(s) 549
TaqI TCGA 1 cut(s) 702
TasI AATT 2 cut(s) 235, 297
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 327, 424, 432
TseI GCWGC 1 cut(s) 575
TspDTI ATGAA 2 cut(s) 151, 624
TspGWI ACGGA 1 cut(s) 816
TspRI CASTG 3 cut(s) 327, 424, 432
Van91I CCANNNNNTGG 1 cut(s) 629
Vha464I CTTAAG 1 cut(s) 368
VneI GTGCAC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 235
XspI CTAG 4 cut(s) 194, 306, 432, 611
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.