Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009602

Physical Location & Seq

Genomic Coordinates Reverse (-)

10815 .. 13359

Gene Structure 2545 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00026.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 810 bp

ATGATGATGATGAATCTTTTAAGGAGATCAGCGATAGCCATCGGCCGCCAATCAAAATCGAAACTGGCGTCGTTTTCCTCCGCTACACAACCTTGTTCCGGAATCCCAAAATCGTCCAAGAGGGTTTTTAGCAACTCATTTCTTTCAAAGGACTCTACTGGAGCTAATGGACTATTGTTTCGCTTCCGAAACCCGCAAGCAAGTATATGTACGGAAGCTCGTCCAAAGAACATCAACTCATCTTACTTTACCAGATCTTTTGCATCAAGAACTTCAAAAGAACCTGGGAATCAGCAGAATAAGGCTAAGAAAGAGGTGACGACTGTGGAGGATCCTTTTGATTCTCCTACATACCACATCCCGGAGAAGCCGGTGACCTTTACAGAAGGTGCGTCCTACAGTCTTGTTATTCTGGCTGGGCTTGGTGTCGCTGGAGCTGCTGGCTACGGTGTGTTTAAAGAGCTCATTTTTCAACCAAAAGAGTACAAGGTCTTTGACAAAGCCTTGAAGAGAATTCAAGATGATGGTCAGGTGAGGGTTAGGATTGGATCCCCCATCAAAGGCTATGGACAAGAAACTAGAAACCGAGCTGCTCGTCAACGTATACCCAACAGAGTATTTACAGATGAAGACGGAGTGGAGCATGTTGAGGTAAACTTCTACATCCGTGGGCCTCAAGGAGCAGGGAAAGTGTACACGGAGATGTTTAAAGACAAGGCAGAGAAGGAATGGAAATACACGTACCTGATCGTAGAGATTTTAACACCTTCCCCAGCCAAATTGATGCTGGAGTCTTATTTGCCTGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0007275 GO:0008104 GO:0008150 GO:0008535 GO:0009791 GO:0009987 GO:0010257 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017004 GO:0017038 GO:0019866 GO:0022607 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032981 GO:0032991 GO:0033036 GO:0033108 GO:0033365 GO:0033617 GO:0034613 GO:0034622 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0065003 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0090351 GO:0098796 GO:0098798 GO:0098800 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TIM21

Protein Analysis

269

Amino Acids

30.05

Weight (kDa)

9.75

Isoelectric Point (pI)

43.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIM21	PF08294	128 - 253	2e-24	TIM21

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00026
fragaria_vesca	FvH4_7g06430
malus_domestica	MD05G1185000.v1.1 MD10G1173000.v1.1
prunus_persica	Prupe.8G211200_v2.0.a1 Prupe.8G211200_v2.0.a1
pyrus_communis	pycom05g16980 pycom10g14910
rosa_chinensis	RchiOBHm_Chr6g0285911
rosa_laevigata	RLG00000012600
rosa_multiflora	Rmu_co8342957.1_g000001 Rmu_co8417589.1_g000001 Rmu_sc0004808.1_g000002
rosa_roxburghii	Rroxscaffold_7G00182620
rosa_rugosa	Rorug06G0177500 Rorug06G0177500
rosa_samantha	Rh6AG289500 Rh6BG285600 Rh6BG291300 Rh6CG292300 Rh6DG284400
rosa_wichuraiana	Rw6G024940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	604
AccIII	TCCGGA	1 cut(s)	98
AciI	CCGC	3 cut(s)	46, 81, 194
AclWI	GGATC	4 cut(s)	326, 339, 543, 556
AcoI	YGGCCR	1 cut(s)	43
AcsI	RAATTY	1 cut(s)	513
AcyI	GRCGYC	1 cut(s)	68
AfaI	GTAC	4 cut(s)	211, 485, 695, 743
AfiI	CCNNNNNNNGG	3 cut(s)	98, 361, 560
AflIII	ACRYGT	1 cut(s)	738
AgsI	TTSAA	5 cut(s)	147, 276, 473, 508, 518
AjnI	CCWGG	1 cut(s)	283
AluBI	AGCT	5 cut(s)	164, 218, 437, 463, 590
AluI	AGCT	5 cut(s)	164, 218, 437, 463, 590
Alw21I	GWGCWC	1 cut(s)	465
AlwI	GGATC	4 cut(s)	326, 339, 543, 556
Aor13HI	TCCGGA	1 cut(s)	98
AoxI	GGCC	2 cut(s)	43, 671
ApeKI	GCWGC	2 cut(s)	437, 590
ApoI	RAATTY	1 cut(s)	513
ArsI	GACNNNNNNTTYG	4 cut(s)	53, 85, 162, 194
AspS9I	GGNCC	1 cut(s)	671
AsuC2I	CCSGG	1 cut(s)	362
AsuHPI	GGTGA	3 cut(s)	328, 385, 544
BamHI	GGATCC	2 cut(s)	331, 548
BanII	GRGCYC	1 cut(s)	465
BbsI	GAAGAC	1 cut(s)	636
Bbv12I	GWGCWC	1 cut(s)	465
BbvI	GCAGC	2 cut(s)	424, 577
BccI	CCATC	3 cut(s)	47, 518, 563
BciT130I	CCWGG	1 cut(s)	285
BcnI	CCSGG	1 cut(s)	362
BfaI	CTAG	1 cut(s)	579
BfmI	CTRYAG	1 cut(s)	397
BglII	AGATCT	1 cut(s)	254
BisI	GCNGC	3 cut(s)	46, 438, 591
BlsI	GCNGC	3 cut(s)	47, 439, 592
Bme1390I	CCNGG	2 cut(s)	285, 362
BmgT120I	GGNCC	1 cut(s)	671
BmiI	GGNNCC	2 cut(s)	333, 550
BmrFI	CCNGG	2 cut(s)	285, 362
BmsI	GCATC	2 cut(s)	272, 774
BpiI	GAAGAC	1 cut(s)	636
BpmI	CTGGAG	3 cut(s)	180, 453, 809
BpuEI	CTTGAG	1 cut(s)	660
BpuMI	CCSGG	1 cut(s)	362
BsaAI	YACGTR	1 cut(s)	741
BsaBI	GATNNNNATC	1 cut(s)	38
BsaHI	GRCGYC	1 cut(s)	68
BsaJI	CCNNGG	2 cut(s)	284, 667
BsaWI	WCCGGW	1 cut(s)	98
Bsc4I	CCNNNNNNNGG	3 cut(s)	98, 361, 560
Bse118I	RCCGGY	1 cut(s)	370
Bse1I	ACTGG	2 cut(s)	69, 163
Bse8I	GATNNNNATC	1 cut(s)	38
BseAI	TCCGGA	1 cut(s)	98
BseBI	CCWGG	1 cut(s)	285
BseDI	CCNNGG	2 cut(s)	284, 667
BseGI	GGATG	2 cut(s)	357, 663
BseJI	GATNNNNATC	1 cut(s)	38
BseLI	CCNNNNNNNGG	3 cut(s)	98, 361, 560
BseNI	ACTGG	2 cut(s)	69, 163
BseX3I	CGGCCG	1 cut(s)	43
BseXI	GCAGC	2 cut(s)	424, 577
BseYI	CCCAGC	2 cut(s)	416, 772
Bsh1285I	CGRYCG	1 cut(s)	46
BshFI	GGCC	2 cut(s)	45, 673
BsiEI	CGRYCG	1 cut(s)	46
BsiHKAI	GWGCWC	1 cut(s)	465
BsiSI	CCGG	3 cut(s)	99, 362, 371
BslI	CCNNNNNNNGG	3 cut(s)	98, 361, 560
BsnI	GGCC	2 cut(s)	45, 673
Bsp1286I	GDGCHC	1 cut(s)	465
Bsp13I	TCCGGA	1 cut(s)	98
Bsp1407I	TGTACA	1 cut(s)	693
Bsp143I	GATC	5 cut(s)	26, 254, 331, 548, 747
BspACI	CCGC	3 cut(s)	46, 81, 194
BspANI	GGCC	2 cut(s)	45, 673
BspEI	TCCGGA	1 cut(s)	98
BspLI	GGNNCC	2 cut(s)	333, 550
BspPI	GGATC	4 cut(s)	326, 339, 543, 556
BsrFI	RCCGGY	1 cut(s)	370
BsrGI	TGTACA	1 cut(s)	693
BsrI	ACTGG	2 cut(s)	69, 163
BssAI	RCCGGY	1 cut(s)	370
BssECI	CCNNGG	2 cut(s)	284, 667
BssMI	GATC	5 cut(s)	26, 254, 331, 548, 747
BssNAI	GTATAC	1 cut(s)	605
BssNI	GRCGYC	1 cut(s)	68
Bst1107I	GTATAC	1 cut(s)	605
Bst2UI	CCWGG	1 cut(s)	285
Bst4CI	ACNGT	3 cut(s)	325, 401, 449
Bst6I	CTCTTC	1 cut(s)	503
BstACI	GRCGYC	1 cut(s)	68
BstAUI	TGTACA	1 cut(s)	693
BstBAI	YACGTR	1 cut(s)	741
BstC8I	GCNNGC	3 cut(s)	198, 442, 804
BstDEI	CTNAG	1 cut(s)	306
BstDSI	CCRYGG	1 cut(s)	667
BstEII	GGTNACC	1 cut(s)	373
BstF5I	GGATG	2 cut(s)	357, 663
BstKTI	GATC	5 cut(s)	29, 257, 334, 551, 750
BstMBI	GATC	5 cut(s)	26, 254, 331, 548, 747
BstMCI	CGRYCG	1 cut(s)	46
BstMWI	GCNNNNNNNGC	1 cut(s)	437
BstNI	CCWGG	1 cut(s)	285
BstNSI	RCATGY	1 cut(s)	647
BstPI	GGTNACC	1 cut(s)	373
BstSCI	CCNGG	2 cut(s)	283, 360
BstSFI	CTRYAG	1 cut(s)	397
BstV1I	GCAGC	2 cut(s)	424, 577
BstV2I	GAAGAC	1 cut(s)	636
BstX2I	RGATCY	3 cut(s)	254, 331, 548
BstYI	RGATCY	3 cut(s)	254, 331, 548
BstZ17I	GTATAC	1 cut(s)	605
BstZI	CGGCCG	1 cut(s)	43
BsuRI	GGCC	2 cut(s)	45, 673
BtgI	CCRYGG	1 cut(s)	667
BtsCI	GGATG	2 cut(s)	357, 663
Cac8I	GCNNGC	3 cut(s)	198, 442, 804
Cfr10I	RCCGGY	1 cut(s)	370
Cfr13I	GGNCC	1 cut(s)	671
CseI	GACGC	2 cut(s)	57, 381
Csp6I	GTAC	4 cut(s)	210, 484, 694, 742
CviAII	CATG	1 cut(s)	644
CviQI	GTAC	4 cut(s)	210, 484, 694, 742
DdeI	CTNAG	1 cut(s)	306
DpnI	GATC	5 cut(s)	28, 256, 333, 550, 749
DpnII	GATC	5 cut(s)	26, 254, 331, 548, 747
DraI	TTTAAA	2 cut(s)	457, 709
EaeI	YGGCCR	1 cut(s)	43
EagI	CGGCCG	1 cut(s)	43
Eam1104I	CTCTTC	1 cut(s)	503
EarI	CTCTTC	1 cut(s)	503
Ecl136II	GAGCTC	1 cut(s)	463
EclXI	CGGCCG	1 cut(s)	43
Eco24I	GRGCYC	1 cut(s)	465
Eco52I	CGGCCG	1 cut(s)	43
Eco53kI	GAGCTC	1 cut(s)	463
Eco91I	GGTNACC	1 cut(s)	373
EcoICRI	GAGCTC	1 cut(s)	463
EcoO65I	GGTNACC	1 cut(s)	373
EcoRI	GAATTC	1 cut(s)	513
EcoRII	CCWGG	1 cut(s)	283
EcoT38I	GRGCYC	1 cut(s)	465
FaeI	CATG	1 cut(s)	647
FaiI	YATR	6 cut(s)	206, 208, 352, 567, 605, 645
FatI	CATG	1 cut(s)	643
FauI	CCCGC	1 cut(s)	201
FblI	GTMKAC	1 cut(s)	604
Fnu4HI	GCNGC	3 cut(s)	46, 438, 591
FokI	GGATG	2 cut(s)	344, 650
FriOI	GRGCYC	1 cut(s)	465
Fsp4HI	GCNGC	3 cut(s)	46, 438, 591
FspBI	CTAG	1 cut(s)	579
GluI	GCNGC	3 cut(s)	46, 438, 591
GsaI	CCCAGC	2 cut(s)	420, 776
GsuI	CTGGAG	3 cut(s)	180, 453, 809
HaeIII	GGCC	2 cut(s)	45, 673
HapII	CCGG	3 cut(s)	99, 362, 371
HgaI	GACGC	2 cut(s)	57, 381
Hin1I	GRCGYC	1 cut(s)	68
Hin1II	CATG	1 cut(s)	647
HincII	GTYRAC	1 cut(s)	599
HindII	GTYRAC	1 cut(s)	599
HinfI	GANTC	6 cut(s)	13, 102, 152, 289, 341, 791
HpaII	CCGG	3 cut(s)	99, 362, 371
HphI	GGTGA	3 cut(s)	328, 385, 544
Hpy166II	GTNNAC	5 cut(s)	599, 605, 655, 694, 696
Hpy188I	TCNGA	1 cut(s)	188
Hpy188III	TCNNGA	3 cut(s)	99, 267, 518
Hpy8I	GTNNAC	5 cut(s)	599, 605, 655, 694, 696
Hpy99I	CGWCG	1 cut(s)	73
HpyAV	CCTTC	3 cut(s)	380, 718, 777
HpyCH4III	ACNGT	3 cut(s)	325, 401, 449
HpyCH4IV	ACGT	2 cut(s)	601, 740
HpyCH4V	TGCA	1 cut(s)	263
HpyF10VI	GCNNNNNNNGC	1 cut(s)	437
HpyF3I	CTNAG	1 cut(s)	306
HpySE526I	ACGT	2 cut(s)	601, 740
Hsp92I	GRCGYC	1 cut(s)	68
Hsp92II	CATG	1 cut(s)	647
Kpn2I	TCCGGA	1 cut(s)	98
Kzo9I	GATC	5 cut(s)	26, 254, 331, 548, 747
LmnI	GCTCC	4 cut(s)	161, 434, 640, 680
Lsp1109I	GCAGC	2 cut(s)	424, 577
LweI	GCATC	2 cut(s)	272, 774
MaeI	CTAG	1 cut(s)	579
MaeII	ACGT	2 cut(s)	601, 740
MaeIII	GTNAC	2 cut(s)	316, 373
MalI	GATC	5 cut(s)	28, 256, 333, 550, 749
MboI	GATC	5 cut(s)	26, 254, 331, 548, 747
MboII	GAAGA	2 cut(s)	520, 641
MflI	RGATCY	3 cut(s)	254, 331, 548
MhlI	GDGCHC	1 cut(s)	465
MluCI	AATT	2 cut(s)	513, 779
MlyI	GAGTC	2 cut(s)	146, 800
MnlI	CCTC	7 cut(s)	88, 114, 307, 322, 528, 643, 684
MroI	TCCGGA	1 cut(s)	98
MseI	TTAA	4 cut(s)	20, 456, 708, 761
MslI	CAYNNNNRTG	1 cut(s)	701
MspI	CCGG	3 cut(s)	99, 362, 371
MspR9I	CCNGG	2 cut(s)	285, 362
MvaI	CCWGG	1 cut(s)	285
MwoI	GCNNNNNNNGC	1 cut(s)	437
NciI	CCSGG	1 cut(s)	362
NdeII	GATC	5 cut(s)	26, 254, 331, 548, 747
NlaIII	CATG	1 cut(s)	647
NlaIV	GGNNCC	2 cut(s)	333, 550
NmuCI	GTSAC	2 cut(s)	316, 373
NspI	RCATGY	1 cut(s)	647
PcsI	WCGNNNNNNNCGW	1 cut(s)	65
PfeI	GAWTC	4 cut(s)	13, 102, 289, 341
PfoI	TCCNGGA	1 cut(s)	360
PkrI	GCNGC	3 cut(s)	47, 439, 592
PleI	GAGTC	2 cut(s)	146, 799
PpsI	GAGTC	2 cut(s)	146, 799
Ppu21I	YACGTR	1 cut(s)	741
Psp124BI	GAGCTC	1 cut(s)	465
Psp6I	CCWGG	1 cut(s)	283
PspEI	GGTNACC	1 cut(s)	373
PspFI	CCCAGC	2 cut(s)	416, 772
PspGI	CCWGG	1 cut(s)	283
PspN4I	GGNNCC	2 cut(s)	333, 550
PspPI	GGNCC	1 cut(s)	671
PsuI	RGATCY	3 cut(s)	254, 331, 548
RsaI	GTAC	4 cut(s)	211, 485, 695, 743
RsaNI	GTAC	4 cut(s)	210, 484, 694, 742
RseI	CAYNNNNRTG	1 cut(s)	701
SacI	GAGCTC	1 cut(s)	465
SaqAI	TTAA	4 cut(s)	20, 456, 708, 761
SatI	GCNGC	3 cut(s)	46, 438, 591
Sau3AI	GATC	5 cut(s)	26, 254, 331, 548, 747
Sau96I	GGNCC	1 cut(s)	671
SchI	GAGTC	2 cut(s)	146, 800
ScrFI	CCNGG	2 cut(s)	285, 362
SduI	GDGCHC	1 cut(s)	465
SfaNI	GCATC	2 cut(s)	272, 774
SfcI	CTRYAG	1 cut(s)	397
SmiMI	CAYNNNNRTG	1 cut(s)	701
SmlI	CTYRAG	1 cut(s)	675
SmoI	CTYRAG	1 cut(s)	675
Sse9I	AATT	2 cut(s)	513, 779
SsiI	CCGC	3 cut(s)	46, 81, 194
SspMI	CTAG	1 cut(s)	579
SstI	GAGCTC	1 cut(s)	465
StyD4I	CCNGG	2 cut(s)	283, 360
TaaI	ACNGT	3 cut(s)	325, 401, 449
TaiI	ACGT	2 cut(s)	604, 743
TaqI	TCGA	1 cut(s)	59
TasI	AATT	2 cut(s)	513, 779
TatI	WGTACW	2 cut(s)	483, 693
TauI	GCSGC	1 cut(s)	48
TfiI	GAWTC	4 cut(s)	13, 102, 289, 341
Tru1I	TTAA	4 cut(s)	20, 456, 708, 761
Tru9I	TTAA	4 cut(s)	20, 456, 708, 761
TseFI	GTSAC	2 cut(s)	316, 373
TseI	GCWGC	2 cut(s)	437, 590
Tsp45I	GTSAC	2 cut(s)	316, 373
TspDTI	ATGAA	2 cut(s)	26, 642
TspGWI	ACGGA	4 cut(s)	227, 648, 656, 713
XapI	RAATTY	1 cut(s)	513
XceI	RCATGY	1 cut(s)	647
XcmI	CCANNNNNNNNNTGG	1 cut(s)	784
XmiI	GTMKAC	1 cut(s)	604
XspI	CTAG	1 cut(s)	579

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.