Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0009602

Physical Location & Seq

Genomic Coordinates Reverse (-)

31353588 .. 31356687

Gene Structure 3100 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1185000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 906 bp

ATGGGGTCGTACAAGTGTGCTATGGACTTATGGAGGAAAAATCAAGTAGATTTGATTAGGGTTTTGCCCAGGATGAGATTGTGGGGGCAGAGGGCTTTCCACACAGAGGAGGTCAAGGCTGATCAGTTGCTGGCAGTAGTGAGATCAGGTTTTGCAAGTCAATCTGCTGCTCCTGGAAGCCATTTGTCCAGGGTGAGGGCGTGGCCGGAGCTAATGGTTGGAATTCCTGTTCTATATGGAAGGAATCCCGCCAAAGCGTTTATGCATGCTTCTTGGCAACAGTCACAACTTACAAGAAACTATGGAGAGAGTACCACCATTCCACTCTTTAGCAGATCCTTTTCATCAAAAGCTACACGGAAACAACAACAAAGTTCATCTGAGAATAAGAAAGACGTATCTACTGTAGAGGATCCTTTTGATGCTCCTACATACAACATCCCAGAAAAGCCTGTGACATTTGTAGAGGGAGCTTCCTACAGTCTTGTCATTCTTGCAGGGCTTGGAGTTGCTGTTGCGGCAGGATATGCTGTTTTCAAGGAGCTTATTTTTGAACCAAAAGAGTACAAGATCTTTAACAAGGCTCTTAAAAGGATTCAAGATGATGCACAGGTTAGGATGAGGGTCGGATATCCTATTACTGGATACGGTCAAGAAAGTAGAAATCGTGCTGCTCGCCAACGCATTCCCAACAGAATATGGCACGATGAAGAAGGCGTAGAGCATGTAGAGGTAAACTTTCATGTTCGTGGGCCGCATGGAGCCGGGAAAGTATTTGCGGAGATGTTCAAAGACGGAACAGACAAGCAATGGAAGTTCACATATTTGATCGTTCAGATACAGTCACCTTCTCCGGCACAGTTGATTCTGGAGTCTTATCTGCCTGCTTACAACGCAGCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0007275 GO:0008104 GO:0008150 GO:0008535 GO:0009791 GO:0009987 GO:0010257 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017004 GO:0017038 GO:0019866 GO:0022607 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032981 GO:0032991 GO:0033036 GO:0033108 GO:0033365 GO:0033617 GO:0034613 GO:0034622 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0065003 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0090351 GO:0098796 GO:0098798 GO:0098800 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TIM21

Protein Analysis

302

Amino Acids

34.01

Weight (kDa)

9.57

Isoelectric Point (pI)

47.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIM21	PF08294	157 - 282	1.4e-23	TIM21

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00026
fragaria_vesca	FvH4_7g06430
malus_domestica	MD05G1185000.v1.1 MD10G1173000.v1.1
prunus_persica	Prupe.8G211200_v2.0.a1 Prupe.8G211200_v2.0.a1
pyrus_communis	pycom05g16980 pycom10g14910
rosa_chinensis	RchiOBHm_Chr6g0285911
rosa_laevigata	RLG00000012600
rosa_multiflora	Rmu_co8342957.1_g000001 Rmu_co8417589.1_g000001 Rmu_sc0004808.1_g000002
rosa_roxburghii	Rroxscaffold_7G00182620
rosa_rugosa	Rorug06G0177500 Rorug06G0177500
rosa_samantha	Rh6AG289500 Rh6BG285600 Rh6BG291300 Rh6CG292300 Rh6DG284400
rosa_wichuraiana	Rw6G024940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	4 cut(s)	249, 518, 755, 779
AclWI	GGATC	3 cut(s)	330, 407, 420
AcoI	YGGCCR	1 cut(s)	203
AcsI	RAATTY	1 cut(s)	222
AfaI	GTAC	3 cut(s)	11, 313, 566
AfiI	CCNNNNNNNGG	3 cut(s)	106, 195, 641
AgsI	TTSAA	4 cut(s)	538, 554, 599, 790
AjnI	CCWGG	3 cut(s)	68, 172, 188
AloI	GAACNNNNNNTCC	2 cut(s)	213, 245
AluBI	AGCT	4 cut(s)	211, 353, 473, 544
AluI	AGCT	4 cut(s)	211, 353, 473, 544
AlwI	GGATC	3 cut(s)	330, 407, 420
AlwNI	CAGNNNCTG	1 cut(s)	130
AoxI	GGCC	2 cut(s)	203, 752
ApeKI	GCWGC	3 cut(s)	167, 671, 896
ApoI	RAATTY	1 cut(s)	222
AspS9I	GGNCC	1 cut(s)	752
AsuC2I	CCSGG	1 cut(s)	766
AsuHPI	GGTGA	2 cut(s)	205, 837
BamHI	GGATCC	1 cut(s)	412
BbvI	GCAGC	2 cut(s)	154, 658
BciT130I	CCWGG	3 cut(s)	70, 174, 190
BciVI	GTATCC	1 cut(s)	638
BclI	TGATCA	1 cut(s)	121
BcnI	CCSGG	1 cut(s)	766
BfaI	CTAG	1 cut(s)	904
BfmI	CTRYAG	2 cut(s)	405, 478
BfuI	GTATCC	1 cut(s)	638
BglII	AGATCT	1 cut(s)	570
BisI	GCNGC	5 cut(s)	168, 519, 672, 755, 897
BlsI	GCNGC	5 cut(s)	169, 520, 673, 756, 898
Bme1390I	CCNGG	4 cut(s)	70, 174, 190, 766
BmgT120I	GGNCC	1 cut(s)	752
BmiI	GGNNCC	2 cut(s)	414, 763
BmrFI	CCNGG	4 cut(s)	70, 174, 190, 766
BmsI	GCATC	2 cut(s)	412, 595
BpmI	CTGGAG	1 cut(s)	890
BpuMI	CCSGG	1 cut(s)	766
BsaJI	CCNNGG	2 cut(s)	68, 189
BsaXI	ACNNNNNCTCC	2 cut(s)	498, 528
Bsc4I	CCNNNNNNNGG	3 cut(s)	106, 195, 641
Bse1I	ACTGG	1 cut(s)	646
Bse3DI	GCAATG	1 cut(s)	815
BseBI	CCWGG	3 cut(s)	70, 174, 190
BseDI	CCNNGG	2 cut(s)	68, 189
BseGI	GGATG	3 cut(s)	78, 438, 624
BseLI	CCNNNNNNNGG	3 cut(s)	106, 195, 641
BseMI	GCAATG	1 cut(s)	815
BseMII	CTCAG	1 cut(s)	372
BseNI	ACTGG	1 cut(s)	646
BseRI	GAGGAG	1 cut(s)	122
BseXI	GCAGC	2 cut(s)	154, 658
BshFI	GGCC	2 cut(s)	205, 754
BsiSI	CCGG	3 cut(s)	206, 765, 854
BslI	CCNNNNNNNGG	3 cut(s)	106, 195, 641
BsmI	GAATGC	1 cut(s)	684
BsnI	GGCC	2 cut(s)	205, 754
Bsp143I	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
BspACI	CCGC	4 cut(s)	249, 518, 755, 779
BspANI	GGCC	2 cut(s)	205, 754
BspCNI	CTCAG	1 cut(s)	373
BspLI	GGNNCC	2 cut(s)	414, 763
BspPI	GGATC	3 cut(s)	330, 407, 420
BsrDI	GCAATG	1 cut(s)	815
BsrI	ACTGG	1 cut(s)	646
BssECI	CCNNGG	2 cut(s)	68, 189
BssMI	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
Bst2UI	CCWGG	3 cut(s)	70, 174, 190
Bst4CI	ACNGT	6 cut(s)	282, 406, 482, 650, 843, 861
BstAPI	GCANNNNNTGC	1 cut(s)	527
BstC8I	GCNNGC	4 cut(s)	132, 267, 676, 885
BstDEI	CTNAG	1 cut(s)	381
BstF5I	GGATG	3 cut(s)	78, 438, 624
BstKTI	GATC	6 cut(s)	124, 146, 338, 415, 573, 831
BstMBI	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
BstMWI	GCNNNNNNNGC	3 cut(s)	518, 527, 893
BstNI	CCWGG	3 cut(s)	70, 174, 190
BstNSI	RCATGY	2 cut(s)	269, 728
BstSCI	CCNGG	4 cut(s)	68, 172, 188, 764
BstSFI	CTRYAG	2 cut(s)	405, 478
BstV1I	GCAGC	2 cut(s)	154, 658
BstX2I	RGATCY	3 cut(s)	335, 412, 570
BstYI	RGATCY	3 cut(s)	335, 412, 570
BsuI	GTATCC	1 cut(s)	638
BsuRI	GGCC	2 cut(s)	205, 754
BtsCI	GGATG	3 cut(s)	78, 438, 624
Cac8I	GCNNGC	4 cut(s)	132, 267, 676, 885
CaiI	CAGNNNCTG	1 cut(s)	130
Cfr13I	GGNCC	1 cut(s)	752
Csp6I	GTAC	3 cut(s)	10, 312, 565
CviAII	CATG	4 cut(s)	266, 725, 743, 758
CviQI	GTAC	3 cut(s)	10, 312, 565
DdeI	CTNAG	1 cut(s)	381
DpnI	GATC	6 cut(s)	123, 145, 337, 414, 572, 830
DpnII	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
EaeI	YGGCCR	1 cut(s)	203
Eco32I	GATATC	1 cut(s)	632
EcoRI	GAATTC	1 cut(s)	222
EcoRII	CCWGG	3 cut(s)	68, 172, 188
EcoRV	GATATC	1 cut(s)	632
EcoT22I	ATGCAT	1 cut(s)	267
FaeI	CATG	4 cut(s)	269, 728, 746, 761
FatI	CATG	4 cut(s)	265, 724, 742, 757
FauI	CCCGC	1 cut(s)	256
FbaI	TGATCA	1 cut(s)	121
Fnu4HI	GCNGC	5 cut(s)	168, 519, 672, 755, 897
FokI	GGATG	3 cut(s)	85, 425, 631
Fsp4HI	GCNGC	5 cut(s)	168, 519, 672, 755, 897
FspBI	CTAG	1 cut(s)	904
GluI	GCNGC	5 cut(s)	168, 519, 672, 755, 897
GsuI	CTGGAG	1 cut(s)	890
HaeIII	GGCC	2 cut(s)	205, 754
HapII	CCGG	3 cut(s)	206, 765, 854
Hin1II	CATG	4 cut(s)	269, 728, 746, 761
HinfI	GANTC	4 cut(s)	244, 595, 865, 872
HpaII	CCGG	3 cut(s)	206, 765, 854
HphI	GGTGA	2 cut(s)	205, 837
Hpy166II	GTNNAC	2 cut(s)	736, 819
Hpy188I	TCNGA	3 cut(s)	382, 629, 837
Hpy188III	TCNNGA	3 cut(s)	599, 653, 869
Hpy8I	GTNNAC	2 cut(s)	736, 819
HpyAV	CCTTC	3 cut(s)	234, 707, 858
HpyCH4III	ACNGT	6 cut(s)	282, 406, 482, 650, 843, 861
HpyCH4IV	ACGT	1 cut(s)	396
HpyCH4V	TGCA	4 cut(s)	155, 265, 497, 608
HpyF10VI	GCNNNNNNNGC	3 cut(s)	518, 527, 893
HpyF3I	CTNAG	1 cut(s)	381
HpySE526I	ACGT	1 cut(s)	396
Hsp92II	CATG	4 cut(s)	269, 728, 746, 761
Ksp22I	TGATCA	1 cut(s)	121
Kzo9I	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
LmnI	GCTCC	6 cut(s)	175, 208, 430, 470, 541, 761
Lsp1109I	GCAGC	2 cut(s)	154, 658
LweI	GCATC	2 cut(s)	412, 595
MaeI	CTAG	1 cut(s)	904
MaeII	ACGT	1 cut(s)	396
MaeIII	GTNAC	3 cut(s)	282, 454, 843
MalI	GATC	6 cut(s)	123, 145, 337, 414, 572, 830
MboI	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
MboII	GAAGA	1 cut(s)	722
MflI	RGATCY	3 cut(s)	335, 412, 570
MluCI	AATT	1 cut(s)	222
MlyI	GAGTC	1 cut(s)	881
MmeI	TCCRAC	2 cut(s)	199, 607
MnlI	CCTC	9 cut(s)	27, 84, 100, 103, 189, 403, 460, 615, 724
Mph1103I	ATGCAT	1 cut(s)	267
MseI	TTAA	2 cut(s)	576, 588
MslI	CAYNNNNRTG	1 cut(s)	747
MspI	CCGG	3 cut(s)	206, 765, 854
MspR9I	CCNGG	4 cut(s)	70, 174, 190, 766
Mva1269I	GAATGC	1 cut(s)	684
MvaI	CCWGG	3 cut(s)	70, 174, 190
MwoI	GCNNNNNNNGC	3 cut(s)	518, 527, 893
NciI	CCSGG	1 cut(s)	766
NdeII	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
NlaIII	CATG	4 cut(s)	269, 728, 746, 761
NlaIV	GGNNCC	2 cut(s)	414, 763
NmuCI	GTSAC	3 cut(s)	282, 454, 843
NsiI	ATGCAT	1 cut(s)	267
NspI	RCATGY	2 cut(s)	269, 728
PaeI	GCATGC	1 cut(s)	269
PctI	GAATGC	1 cut(s)	684
PfeI	GAWTC	3 cut(s)	244, 595, 865
PfoI	TCCNGGA	1 cut(s)	172
PkrI	GCNGC	5 cut(s)	169, 520, 673, 756, 898
PleI	GAGTC	1 cut(s)	880
PpsI	GAGTC	1 cut(s)	880
Psp6I	CCWGG	3 cut(s)	68, 172, 188
PspGI	CCWGG	3 cut(s)	68, 172, 188
PspN4I	GGNNCC	2 cut(s)	414, 763
PspPI	GGNCC	1 cut(s)	752
PstNI	CAGNNNCTG	1 cut(s)	130
PsuI	RGATCY	3 cut(s)	335, 412, 570
RsaI	GTAC	3 cut(s)	11, 313, 566
RsaNI	GTAC	3 cut(s)	10, 312, 565
RseI	CAYNNNNRTG	1 cut(s)	747
SaqAI	TTAA	2 cut(s)	576, 588
SatI	GCNGC	5 cut(s)	168, 519, 672, 755, 897
Sau3AI	GATC	6 cut(s)	121, 143, 335, 412, 570, 828
Sau96I	GGNCC	1 cut(s)	752
SchI	GAGTC	1 cut(s)	881
ScrFI	CCNGG	4 cut(s)	70, 174, 190, 766
SfaNI	GCATC	2 cut(s)	412, 595
SfcI	CTRYAG	2 cut(s)	405, 478
SmiMI	CAYNNNNRTG	1 cut(s)	747
SphI	GCATGC	1 cut(s)	269
Sse9I	AATT	1 cut(s)	222
SsiI	CCGC	4 cut(s)	249, 518, 755, 779
SspMI	CTAG	1 cut(s)	904
StyD4I	CCNGG	4 cut(s)	68, 172, 188, 764
TaaI	ACNGT	6 cut(s)	282, 406, 482, 650, 843, 861
TaiI	ACGT	1 cut(s)	399
TasI	AATT	1 cut(s)	222
TatI	WGTACW	1 cut(s)	564
TauI	GCSGC	2 cut(s)	521, 757
TfiI	GAWTC	3 cut(s)	244, 595, 865
Tru1I	TTAA	2 cut(s)	576, 588
Tru9I	TTAA	2 cut(s)	576, 588
TseFI	GTSAC	3 cut(s)	282, 454, 843
TseI	GCWGC	3 cut(s)	167, 671, 896
Tsp45I	GTSAC	3 cut(s)	282, 454, 843
TspDTI	ATGAA	4 cut(s)	333, 366, 723, 731
TspGWI	ACGGA	2 cut(s)	373, 810
XapI	RAATTY	1 cut(s)	222
XceI	RCATGY	2 cut(s)	269, 728
XspI	CTAG	1 cut(s)	904
Zsp2I	ATGCAT	1 cut(s)	267

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.