Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009602

Physical Location & Seq

Genomic Coordinates Reverse (-)

26565732 .. 26568958

Gene Structure 3227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1173000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 897 bp

ATGGGATCCTACAAGTGTGCAATGGAGTTATTGAGGAAAAATCAAGTAGATTTGATTAGGGTTTTGCCCAGGATGAGATTGAGGGGGCAGCGGGCTTTCCACACAGAGGAGGTCAAGGCAGATCAGTTGCTGGCAGTAGTAAGATCAGGTTTTGCGAGTCAATCTGCTGCTCCCGGAAGCCACTTGACTAGGGGCGTAGCCAGAGCTAATGGAATTCCTGTTCTATATGGAAGGTATCCTGCCAGAGAGTTTATGCATGCTTCTTGGCCACAGTCACAACTCATAAGAAACTACAGAGCAAGTACCACCATTCCTCTCTTTAGCAGATCCTTTTCATCAAAAGCTACACGGAAACAGCAACAAAGTTCATCTGAGAATAAGAAAGATGTATCTACTATAGAGGATCCTTTTGATGCTCCTACATACAACATCCCAGAAAAGCCTGTGACGTTTGTAGAGGGAGCTTCCTACAGTCTTGTCATTCTTGCAGGGCTTGGAATTGCTGTTGCTGCAGGATATGCTGTTTTCAAGGAGCTTATTTTTGAACCAAAAGAGTACAAGATCTTTAACAAGGCTCTTAAAAGGATTCAAGATGATGCACAGGTTAGGATGAGGGTCGGATATCCTATTACTGGATACGGTCAAGAAAGTAGAAACCGTGCTGCTCGCCAACGCATTCCCAACAGAGTATGGCACGATGAAGAAGGCGTAGAGCATGTAGAGGTTAACTTTCATGTTCGCGGACCCCATGGAGCCGGGAAAGTATTTGCGGAGATGTTCAAAGACGGAACAGACAATCAATGGAAGTTCACGTATTTGATTGTTCAGATACAATCACCTTCTCCGACACAATTGATTCTAGAGTCTTATCTGCCTTCTTACAACACAGCCAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006605 GO:0006626 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0007275 GO:0008104 GO:0008150 GO:0008535 GO:0009791 GO:0009987 GO:0010257 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0017004 GO:0017038 GO:0019866 GO:0022607 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032981 GO:0032991 GO:0033036 GO:0033108 GO:0033365 GO:0033617 GO:0034613 GO:0034622 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0065003 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0090351 GO:0098796 GO:0098798 GO:0098800 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TIM21

Protein Analysis

299

Amino Acids

33.66

Weight (kDa)

9.72

Isoelectric Point (pI)

44.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIM21	PF08294	154 - 279	7.4e-24	TIM21

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00026
fragaria_vesca	FvH4_7g06430
malus_domestica	MD05G1185000.v1.1 MD10G1173000.v1.1
prunus_persica	Prupe.8G211200_v2.0.a1 Prupe.8G211200_v2.0.a1
pyrus_communis	pycom05g16980 pycom10g14910
rosa_chinensis	RchiOBHm_Chr6g0285911
rosa_laevigata	RLG00000012600
rosa_multiflora	Rmu_co8342957.1_g000001 Rmu_co8417589.1_g000001 Rmu_sc0004808.1_g000002
rosa_roxburghii	Rroxscaffold_7G00182620
rosa_rugosa	Rorug06G0177500 Rorug06G0177500
rosa_samantha	Rh6AG289500 Rh6BG285600 Rh6BG291300 Rh6CG292300 Rh6DG284400
rosa_wichuraiana	Rw6G024940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	741
AciI	CCGC	3 cut(s)	91, 741, 770
AclWI	GGATC	4 cut(s)	13, 321, 398, 411
AcoI	YGGCCR	1 cut(s)	266
AcsI	RAATTY	1 cut(s)	213
AfaI	GTAC	2 cut(s)	304, 557
AfiI	CCNNNNNNNGG	2 cut(s)	106, 632
AgsI	TTSAA	4 cut(s)	529, 545, 590, 781
AjnI	CCWGG	1 cut(s)	68
AloI	GAACNNNNNNTCC	2 cut(s)	204, 236
AluBI	AGCT	4 cut(s)	206, 344, 464, 535
AluI	AGCT	4 cut(s)	206, 344, 464, 535
AlwI	GGATC	4 cut(s)	13, 321, 398, 411
AlwNI	CAGNNNCTG	1 cut(s)	130
AoxI	GGCC	1 cut(s)	266
ApeKI	GCWGC	4 cut(s)	88, 167, 509, 662
ApoI	RAATTY	1 cut(s)	213
AspS9I	GGNCC	1 cut(s)	743
AsuC2I	CCSGG	2 cut(s)	174, 757
AsuHPI	GGTGA	1 cut(s)	828
AvaII	GGWCC	1 cut(s)	743
BalI	TGGCCA	1 cut(s)	268
BamHI	GGATCC	2 cut(s)	5, 403
BbvI	GCAGC	4 cut(s)	100, 154, 496, 649
BciT130I	CCWGG	1 cut(s)	70
BciVI	GTATCC	2 cut(s)	246, 629
BcnI	CCSGG	2 cut(s)	174, 757
BfaI	CTAG	3 cut(s)	189, 860, 895
BfmI	CTRYAG	4 cut(s)	292, 396, 469, 510
BfuI	GTATCC	2 cut(s)	246, 629
BglII	AGATCT	1 cut(s)	561
BisI	GCNGC	4 cut(s)	89, 168, 510, 663
BlsI	GCNGC	4 cut(s)	90, 169, 511, 664
Bme1390I	CCNGG	3 cut(s)	70, 174, 757
Bme18I	GGWCC	1 cut(s)	743
BmgT120I	GGNCC	1 cut(s)	743
BmiI	GGNNCC	4 cut(s)	7, 405, 745, 754
BmrFI	CCNGG	3 cut(s)	70, 174, 757
BmsI	GCATC	2 cut(s)	403, 586
BpuMI	CCSGG	2 cut(s)	174, 757
BsaAI	YACGTR	1 cut(s)	813
BsaJI	CCNNGG	2 cut(s)	68, 748
Bsc4I	CCNNNNNNNGG	2 cut(s)	106, 632
Bse1I	ACTGG	1 cut(s)	637
Bse3DI	GCAATG	1 cut(s)	27
BseBI	CCWGG	1 cut(s)	70
BseDI	CCNNGG	2 cut(s)	68, 748
BseGI	GGATG	3 cut(s)	78, 429, 615
BseLI	CCNNNNNNNGG	2 cut(s)	106, 632
BseMI	GCAATG	1 cut(s)	27
BseMII	CTCAG	1 cut(s)	363
BseNI	ACTGG	1 cut(s)	637
BseRI	GAGGAG	1 cut(s)	122
BseXI	GCAGC	4 cut(s)	100, 154, 496, 649
Bsh1236I	CGCG	1 cut(s)	741
BshFI	GGCC	1 cut(s)	268
BsiSI	CCGG	2 cut(s)	174, 756
BslI	CCNNNNNNNGG	2 cut(s)	106, 632
BsmI	GAATGC	1 cut(s)	675
BsnI	GGCC	1 cut(s)	268
Bsp143I	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
Bsp19I	CCATGG	1 cut(s)	748
BspACI	CCGC	3 cut(s)	91, 741, 770
BspANI	GGCC	1 cut(s)	268
BspCNI	CTCAG	1 cut(s)	364
BspFNI	CGCG	1 cut(s)	741
BspLI	GGNNCC	4 cut(s)	7, 405, 745, 754
BspMAI	CTGCAG	1 cut(s)	514
BspPI	GGATC	4 cut(s)	13, 321, 398, 411
BsrDI	GCAATG	1 cut(s)	27
BsrI	ACTGG	1 cut(s)	637
BssECI	CCNNGG	2 cut(s)	68, 748
BssMI	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
BssT1I	CCWWGG	1 cut(s)	748
Bst2UI	CCWGG	1 cut(s)	70
Bst4CI	ACNGT	4 cut(s)	273, 473, 641, 659
BstAPI	GCANNNNNTGC	1 cut(s)	518
BstBAI	YACGTR	1 cut(s)	813
BstC8I	GCNNGC	4 cut(s)	93, 132, 258, 667
BstDEI	CTNAG	1 cut(s)	372
BstDSI	CCRYGG	1 cut(s)	748
BstF5I	GGATG	3 cut(s)	78, 429, 615
BstFNI	CGCG	1 cut(s)	741
BstKTI	GATC	6 cut(s)	8, 124, 146, 329, 406, 564
BstMBI	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
BstMWI	GCNNNNNNNGC	2 cut(s)	509, 518
BstNI	CCWGG	1 cut(s)	70
BstNSI	RCATGY	2 cut(s)	260, 719
BstSCI	CCNGG	3 cut(s)	68, 172, 755
BstSFI	CTRYAG	4 cut(s)	292, 396, 469, 510
BstUI	CGCG	1 cut(s)	741
BstV1I	GCAGC	4 cut(s)	100, 154, 496, 649
BstX2I	RGATCY	4 cut(s)	5, 326, 403, 561
BstYI	RGATCY	4 cut(s)	5, 326, 403, 561
BsuI	GTATCC	2 cut(s)	246, 629
BsuRI	GGCC	1 cut(s)	268
BtgI	CCRYGG	1 cut(s)	748
BtsCI	GGATG	3 cut(s)	78, 429, 615
Cac8I	GCNNGC	4 cut(s)	93, 132, 258, 667
CaiI	CAGNNNCTG	1 cut(s)	130
Cfr13I	GGNCC	1 cut(s)	743
Csp6I	GTAC	2 cut(s)	303, 556
CviAII	CATG	4 cut(s)	257, 716, 734, 749
CviQI	GTAC	2 cut(s)	303, 556
DdeI	CTNAG	1 cut(s)	372
DpnI	GATC	6 cut(s)	7, 123, 145, 328, 405, 563
DpnII	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
EaeI	YGGCCR	1 cut(s)	266
Eco130I	CCWWGG	1 cut(s)	748
Eco32I	GATATC	1 cut(s)	623
Eco47I	GGWCC	1 cut(s)	743
EcoRI	GAATTC	1 cut(s)	213
EcoRII	CCWGG	1 cut(s)	68
EcoRV	GATATC	1 cut(s)	623
EcoT14I	CCWWGG	1 cut(s)	748
EcoT22I	ATGCAT	1 cut(s)	258
ErhI	CCWWGG	1 cut(s)	748
FaeI	CATG	4 cut(s)	260, 719, 737, 752
FatI	CATG	4 cut(s)	256, 715, 733, 748
FauI	CCCGC	1 cut(s)	84
Fnu4HI	GCNGC	4 cut(s)	89, 168, 510, 663
FokI	GGATG	3 cut(s)	85, 416, 622
Fsp4HI	GCNGC	4 cut(s)	89, 168, 510, 663
FspBI	CTAG	3 cut(s)	189, 860, 895
GluI	GCNGC	4 cut(s)	89, 168, 510, 663
HaeIII	GGCC	1 cut(s)	268
HapII	CCGG	2 cut(s)	174, 756
Hin1II	CATG	4 cut(s)	260, 719, 737, 752
HincII	GTYRAC	1 cut(s)	727
HindII	GTYRAC	1 cut(s)	727
HinfI	GANTC	4 cut(s)	157, 586, 856, 863
HpaI	GTTAAC	1 cut(s)	727
HpaII	CCGG	2 cut(s)	174, 756
HphI	GGTGA	1 cut(s)	828
Hpy166II	GTNNAC	2 cut(s)	727, 810
Hpy188I	TCNGA	4 cut(s)	373, 620, 828, 846
Hpy188III	TCNNGA	3 cut(s)	590, 644, 860
Hpy8I	GTNNAC	2 cut(s)	727, 810
HpyAV	CCTTC	4 cut(s)	225, 698, 849, 885
HpyCH4III	ACNGT	4 cut(s)	273, 473, 641, 659
HpyCH4IV	ACGT	2 cut(s)	449, 812
HpyCH4V	TGCA	5 cut(s)	20, 256, 488, 512, 599
HpyF10VI	GCNNNNNNNGC	2 cut(s)	509, 518
HpyF3I	CTNAG	1 cut(s)	372
HpySE526I	ACGT	2 cut(s)	449, 812
Hsp92II	CATG	4 cut(s)	260, 719, 737, 752
KspAI	GTTAAC	1 cut(s)	727
Kzo9I	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
LmnI	GCTCC	5 cut(s)	175, 421, 461, 532, 752
Lsp1109I	GCAGC	4 cut(s)	100, 154, 496, 649
LweI	GCATC	2 cut(s)	403, 586
MaeI	CTAG	3 cut(s)	189, 860, 895
MaeII	ACGT	2 cut(s)	449, 812
MaeIII	GTNAC	2 cut(s)	273, 445
MalI	GATC	6 cut(s)	7, 123, 145, 328, 405, 563
MboI	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
MboII	GAAGA	1 cut(s)	713
MfeI	CAATTG	1 cut(s)	851
MflI	RGATCY	4 cut(s)	5, 326, 403, 561
MlsI	TGGCCA	1 cut(s)	268
MluCI	AATT	3 cut(s)	213, 498, 851
MluNI	TGGCCA	1 cut(s)	268
MlyI	GAGTC	2 cut(s)	166, 872
MmeI	TCCRAC	2 cut(s)	598, 869
MnlI	CCTC	9 cut(s)	27, 75, 100, 103, 324, 394, 451, 606, 715
Mox20I	TGGCCA	1 cut(s)	268
Mph1103I	ATGCAT	1 cut(s)	258
MscI	TGGCCA	1 cut(s)	268
MseI	TTAA	3 cut(s)	567, 579, 726
Msp20I	TGGCCA	1 cut(s)	268
MspA1I	CMGCKG	1 cut(s)	91
MspI	CCGG	2 cut(s)	174, 756
MspR9I	CCNGG	3 cut(s)	70, 174, 757
MunI	CAATTG	1 cut(s)	851
Mva1269I	GAATGC	1 cut(s)	675
MvaI	CCWGG	1 cut(s)	70
MvnI	CGCG	1 cut(s)	741
MwoI	GCNNNNNNNGC	2 cut(s)	509, 518
NciI	CCSGG	2 cut(s)	174, 757
NcoI	CCATGG	1 cut(s)	748
NdeII	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
NlaIII	CATG	4 cut(s)	260, 719, 737, 752
NlaIV	GGNNCC	4 cut(s)	7, 405, 745, 754
NmuCI	GTSAC	2 cut(s)	273, 445
NsiI	ATGCAT	1 cut(s)	258
NspI	RCATGY	2 cut(s)	260, 719
PaeI	GCATGC	1 cut(s)	260
PctI	GAATGC	1 cut(s)	675
PfeI	GAWTC	2 cut(s)	586, 856
PfoI	TCCNGGA	1 cut(s)	172
PkrI	GCNGC	4 cut(s)	90, 169, 511, 664
PleI	GAGTC	2 cut(s)	165, 871
PpsI	GAGTC	2 cut(s)	165, 871
Ppu21I	YACGTR	1 cut(s)	813
Psp6I	CCWGG	1 cut(s)	68
PspGI	CCWGG	1 cut(s)	68
PspN4I	GGNNCC	4 cut(s)	7, 405, 745, 754
PspPI	GGNCC	1 cut(s)	743
PstI	CTGCAG	1 cut(s)	514
PstNI	CAGNNNCTG	1 cut(s)	130
PsuI	RGATCY	4 cut(s)	5, 326, 403, 561
RsaI	GTAC	2 cut(s)	304, 557
RsaNI	GTAC	2 cut(s)	303, 556
SaqAI	TTAA	3 cut(s)	567, 579, 726
SatI	GCNGC	4 cut(s)	89, 168, 510, 663
Sau3AI	GATC	6 cut(s)	5, 121, 143, 326, 403, 561
Sau96I	GGNCC	1 cut(s)	743
SchI	GAGTC	2 cut(s)	166, 872
ScrFI	CCNGG	3 cut(s)	70, 174, 757
SfaNI	GCATC	2 cut(s)	403, 586
SfcI	CTRYAG	4 cut(s)	292, 396, 469, 510
SinI	GGWCC	1 cut(s)	743
SphI	GCATGC	1 cut(s)	260
Sse9I	AATT	3 cut(s)	213, 498, 851
SsiI	CCGC	3 cut(s)	91, 741, 770
SspMI	CTAG	3 cut(s)	189, 860, 895
StyD4I	CCNGG	3 cut(s)	68, 172, 755
StyI	CCWWGG	1 cut(s)	748
TaaI	ACNGT	4 cut(s)	273, 473, 641, 659
TaiI	ACGT	2 cut(s)	452, 815
TasI	AATT	3 cut(s)	213, 498, 851
TatI	WGTACW	1 cut(s)	555
TfiI	GAWTC	2 cut(s)	586, 856
Tru1I	TTAA	3 cut(s)	567, 579, 726
Tru9I	TTAA	3 cut(s)	567, 579, 726
TseFI	GTSAC	2 cut(s)	273, 445
TseI	GCWGC	4 cut(s)	88, 167, 509, 662
Tsp45I	GTSAC	2 cut(s)	273, 445
TspDTI	ATGAA	4 cut(s)	324, 357, 714, 722
TspGWI	ACGGA	2 cut(s)	364, 801
VpaK11BI	GGWCC	1 cut(s)	743
XapI	RAATTY	1 cut(s)	213
XbaI	TCTAGA	1 cut(s)	859
XceI	RCATGY	2 cut(s)	260, 719
XspI	CTAG	3 cut(s)	189, 860, 895
Zsp2I	ATGCAT	1 cut(s)	258

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.