AT4G00290

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005685
Physical Location & Seq
Reverse (-)
122851 .. 125591
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00290.1

Sequence Viewer

Length: 1494 bp
ATGGCCGGAGTTAGGTTATCGCTTCTCAAGTCAATTCAAAGATCAATAAAACCTCATGCCACTGCAAAATCGTGTTCTGGTCTCCTAAACTCTCATGCCAGGGCCTTTACTTGCGGAAACCTTCTTGATGGACCAAAAGCTTCTCCTTCGATGATCTCCTTTTCATCGAACATACGCCTGCATAATGATGCTAAGCCTTTCAATTACTTAGGCCATTCTTCGTACGCACGAGCTTTCAGCTCCAAATCTGATGATTTCGGAAGTATTGTAGCCTCTGGTGTTACTGGCTCTGGTGATGGGAATGGGAATGGGAATGATTGGGTTGAGAAAGCTAAAGATGTGTTGCAGACTAGTGTCGATGCTGTTACTGAGACTGCAAAGAAGACTAAAGATGTCTCTGATGAGATGATCCCTCATGTTCAGCAGTTCCTTGACTCTAACCCTTATCTTAAAGATGTTATAGTTCCTGTTAGTCTCACTATGACTGGTACTTTATTTGCTTGGGTTGTGATGCCTAGGATTCTTAGAAGGTTTCACACTTATGCTATGCAAAGTTCCGCTAAACTGCTTCCCGTTGGCTTCTCTAACGAAGATGTTCCTTATGAGAAAAGCTTTTGGGGAGCTTTAGAGGATCCTGCTCGTTACTTGGTGACCTTCATTGCTTTTGCACAAATTGCTGCAATGGTGGCGCCAACAACTATAGCAGCTCAATACTTTTCCCCGACTGTGAAGGGAGCGGTTATTCTCTCACTTGTGTGGTTCCTATATCGGTGGAAGACCAATGTGATTACCCGAATGTTGTCGGCTAAGAGTTTTGGTGGGCTTGATAGGGAGAAGGTATTGACTTTAGACAAAGTATCATCTGTTGGTCTTTTCGCTATCGGTTTGATGGCTTCTGCTGAAGCATGTGGTGTAGCTGTTCAATCTATCTTGACAGTTGGTGGAGTTGGAGGGGTAGCCACCGCATTTGCTGCCAGAGATATCCTTGGTAATGTGCTGAGTGGTCTGTCAATGCAATTCTCGCGGCCTTTCTCTATGGGTGATACTATCAAAGCTGGATCCGTAGAAGGTCAAGTTATTGAAATGGGACTTACGACAACATCTTTGTTGAATGCAGAAAAATTTCCGGTTTTGGTACCCAATTCACTGTTTTCGAGTCAGGTGATTGTGAATAAGTCACGTGCTCAGTGGCGAGCAATAGCATCCAAGATCCCACTGCAGATCGATGACTTGGATATGATTCCTCAAATATCAAATGAAATCAAGGAAATGTTGAGGTCCAACACAAAAGTTTTCTTAGGAAAAGAAGCTCCACACTGTTACTTATCGCGAGTGGAAAAATCTTTCGCTGAACTTACCATTGGGTGCAATCTGATACGCATGGGAAAAGAAGAGCTATACAATACACAACAAGAGGTCCTTCTTGAGGCGGTGAAGATCATAAAGAAGCACGGTGTGTCGTTAGGTACCACCTGGGACAATTCTACATTGTGA

Protein Analysis

497

Amino Acids

53.88

Weight (kDa)

9.07

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 327 - 394 3.1e-15 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1135, 1466
AccB1I GGYRCC 3 cut(s) 688, 1135, 1466
AccBSI CCGCTC 1 cut(s) 737
AccII CGCG 2 cut(s) 1024, 1330
AciI CCGC 6 cut(s) 114, 558, 737, 963, 1024, 1430
AclWI GGATC 6 cut(s) 403, 626, 639, 1053, 1066, 1204
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1121
AcuI CTGAAG 1 cut(s) 921
AcvI CACGTG 1 cut(s) 1181
AcyI GRCGYC 1 cut(s) 689
AdeI CACNNNGTG 1 cut(s) 1456
AfaI GTAC 4 cut(s) 224, 490, 1137, 1468
AfiI CCNNNNNNNGG 2 cut(s) 12, 1426
AgsI TTSAA 5 cut(s) 38, 202, 923, 1082, 1111
AhlI ACTAGT 1 cut(s) 350
AjnI CCWGG 2 cut(s) 98, 1472
AjuI GAANNNNNNNTTGG 2 cut(s) 1344, 1376
AleI CACNNNNGTG 1 cut(s) 754
Alw21I GWGCWC 1 cut(s) 1186
Alw26I GTCTC 4 cut(s) 86, 365, 400, 479
AlwI GGATC 6 cut(s) 403, 626, 639, 1053, 1066, 1204
AoxI GGCC 4 cut(s) 3, 102, 211, 1025
ApeKI GCWGC 3 cut(s) 677, 704, 971
ApoI RAATTY 1 cut(s) 1121
ArsI GACNNNNNNTTYG 2 cut(s) 1087, 1119
Asp700I GAANNNNTTC 2 cut(s) 594, 1122
Asp718I GGTACC 2 cut(s) 1135, 1466
AspA2I CCTAGG 1 cut(s) 515
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 4 cut(s) 102, 131, 1278, 1417
AsuHPI GGTGA 5 cut(s) 305, 661, 1052, 1174, 1444
AvaII GGWCC 3 cut(s) 131, 1278, 1417
AvrII CCTAGG 1 cut(s) 515
BamHI GGATCC 2 cut(s) 631, 1058
BanI GGYRCC 3 cut(s) 688, 1135, 1466
BauI CACGAG 1 cut(s) 228
BbrPI CACGTG 1 cut(s) 1181
BbsI GAAGAC 2 cut(s) 389, 782
Bbv12I GWGCWC 1 cut(s) 1186
BbvI GCAGC 3 cut(s) 664, 716, 958
BccI CCATC 3 cut(s) 122, 290, 883
BciT130I CCWGG 2 cut(s) 100, 1474
BcoDI GTCTC 4 cut(s) 86, 365, 400, 479
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 2 cut(s) 351, 516
BfmI CTRYAG 2 cut(s) 699, 1217
BfoI RGCGCY 1 cut(s) 692
BisI GCNGC 4 cut(s) 678, 705, 972, 1025
BlnI CCTAGG 1 cut(s) 515
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 4 cut(s) 679, 706, 973, 1026
Bme1390I CCNGG 2 cut(s) 100, 1474
Bme18I GGWCC 3 cut(s) 131, 1278, 1417
BmgT120I GGNCC 4 cut(s) 102, 131, 1278, 1417
BmiI GGNNCC 6 cut(s) 633, 690, 761, 1060, 1137, 1468
BmrFI CCNGG 2 cut(s) 100, 1474
BmsI GCATC 4 cut(s) 178, 349, 501, 1211
BoxI GACNNNNGTC 1 cut(s) 353
BpiI GAAGAC 2 cut(s) 389, 782
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 2 cut(s) 11, 1445
Bsa29I ATCGAT 1 cut(s) 1224
BsaAI YACGTR 1 cut(s) 1181
BsaHI GRCGYC 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 99, 515, 985, 1473
BsaWI WCCGGW 1 cut(s) 1126
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 1426
Bse1I ACTGG 2 cut(s) 289, 490
Bse3DI GCAATG 2 cut(s) 657, 687
BseBI CCWGG 2 cut(s) 100, 1474
BseCI ATCGAT 1 cut(s) 1224
BseDI CCNNGG 4 cut(s) 99, 515, 985, 1473
BseGI GGATG 1 cut(s) 1202
BseLI CCNNNNNNNGG 2 cut(s) 12, 1426
BseMI GCAATG 2 cut(s) 657, 687
BseMII CTCAG 3 cut(s) 360, 989, 1199
BseNI ACTGG 2 cut(s) 289, 490
BseXI GCAGC 3 cut(s) 664, 716, 958
Bsh1236I CGCG 2 cut(s) 1024, 1330
BshFI GGCC 4 cut(s) 5, 104, 213, 1027
BshNI GGYRCC 3 cut(s) 688, 1135, 1466
BshVI ATCGAT 1 cut(s) 1224
BsiHKAI GWGCWC 1 cut(s) 1186
BsiSI CCGG 2 cut(s) 6, 1127
BsiWI CGTACG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 1101, 1490
BslI CCNNNNNNNGG 2 cut(s) 12, 1426
BsmAI GTCTC 4 cut(s) 86, 365, 400, 479
BsmFI GGGAC 2 cut(s) 1101, 1490
BsmI GAATGC 1 cut(s) 1117
BsnI GGCC 4 cut(s) 5, 104, 213, 1027
Bso31I GGTCTC 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 1186
Bsp143I GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp68I TCGCGA 1 cut(s) 1330
BspACI CCGC 6 cut(s) 114, 558, 737, 963, 1024, 1430
BspANI GGCC 4 cut(s) 5, 104, 213, 1027
BspCNI CTCAG 3 cut(s) 361, 990, 1198
BspDI ATCGAT 1 cut(s) 1224
BspFNI CGCG 2 cut(s) 1024, 1330
BspLI GGNNCC 6 cut(s) 633, 690, 761, 1060, 1137, 1468
BspMAI CTGCAG 1 cut(s) 1221
BspPI GGATC 6 cut(s) 403, 626, 639, 1053, 1066, 1204
BspQI GCTCTTC 1 cut(s) 1386
BspT107I GGYRCC 3 cut(s) 688, 1135, 1466
BspTNI GGTCTC 1 cut(s) 86
BsrBI CCGCTC 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 657, 687
BsrI ACTGG 2 cut(s) 289, 490
BssECI CCNNGG 4 cut(s) 99, 515, 985, 1473
BssMI GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
BssNI GRCGYC 1 cut(s) 689
BssSI CACGAG 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 515, 985
Bst2BI CACGAG 1 cut(s) 228
Bst2UI CCWGG 2 cut(s) 100, 1474
Bst4CI ACNGT 5 cut(s) 727, 937, 1149, 1319, 1454
Bst6I CTCTTC 1 cut(s) 1386
BstACI GRCGYC 1 cut(s) 689
BstAPI GCANNNNNTGC 2 cut(s) 674, 971
BstBAI YACGTR 1 cut(s) 1181
BstC8I GCNNGC 2 cut(s) 179, 1194
BstDEI CTNAG 8 cut(s) 192, 208, 369, 524, 807, 998, 1185, 1297
BstEII GGTNACC 1 cut(s) 649
BstENI CCTNNNNNAGG 1 cut(s) 1424
BstF5I GGATG 1 cut(s) 1202
BstFNI CGCG 2 cut(s) 1024, 1330
BstH2I RGCGCY 1 cut(s) 692
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 8 cut(s) 44, 156, 411, 634, 1061, 1212, 1224, 1440
BstMAI GTCTC 4 cut(s) 86, 365, 400, 479
BstMBI GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
BstMWI GCNNNNNNNGC 4 cut(s) 674, 686, 971, 1021
BstNI CCWGG 2 cut(s) 100, 1474
BstNSI RCATGY 1 cut(s) 909
BstPAI GACNNNNGTC 1 cut(s) 353
BstPI GGTNACC 1 cut(s) 649
BstSCI CCNGG 2 cut(s) 98, 1472
BstSFI CTRYAG 2 cut(s) 699, 1217
BstUI CGCG 2 cut(s) 1024, 1330
BstV1I GCAGC 3 cut(s) 664, 716, 958
BstV2I GAAGAC 2 cut(s) 389, 782
BstX2I RGATCY 3 cut(s) 631, 1058, 1209
BstYI RGATCY 3 cut(s) 631, 1058, 1209
Bsu15I ATCGAT 1 cut(s) 1224
BsuRI GGCC 4 cut(s) 5, 104, 213, 1027
BsuTUI ATCGAT 1 cut(s) 1224
BtsCI GGATG 1 cut(s) 1202
BtsI GCAGTG 2 cut(s) 60, 1214
BtsIMutI CAGTG 5 cut(s) 60, 1145, 1193, 1214, 1315
BtuMI TCGCGA 1 cut(s) 1330
Cac8I GCNNGC 2 cut(s) 179, 1194
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 4 cut(s) 102, 131, 1278, 1417
ClaI ATCGAT 1 cut(s) 1224
Csp6I GTAC 4 cut(s) 223, 489, 1136, 1467
CspCI CAANNNNNGTGG 2 cut(s) 949, 984
CviAII CATG 5 cut(s) 56, 95, 416, 906, 1381
CviQI GTAC 4 cut(s) 223, 489, 1136, 1467
DdeI CTNAG 8 cut(s) 192, 208, 369, 524, 807, 998, 1185, 1297
DinI GGCGCC 1 cut(s) 690
DpnI GATC 8 cut(s) 43, 155, 410, 633, 1060, 1211, 1223, 1439
DpnII GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
DraIII CACNNNGTG 1 cut(s) 1456
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 1386
EarI CTCTTC 1 cut(s) 1386
Eco130I CCWWGG 2 cut(s) 515, 985
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 982
Eco47I GGWCC 3 cut(s) 131, 1278, 1417
Eco57I CTGAAG 1 cut(s) 921
Eco72I CACGTG 1 cut(s) 1181
Eco91I GGTNACC 1 cut(s) 649
EcoNI CCTNNNNNAGG 1 cut(s) 1424
EcoO109I RGGNCCY 2 cut(s) 102, 1417
EcoO65I GGTNACC 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 98, 1472
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 2 cut(s) 515, 985
EgeI GGCGCC 1 cut(s) 690
EheI GGCGCC 1 cut(s) 690
ErhI CCWWGG 2 cut(s) 515, 985
FaeI CATG 5 cut(s) 59, 98, 419, 909, 1384
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FaqI GGGAC 2 cut(s) 1101, 1490
FatI CATG 5 cut(s) 55, 94, 415, 905, 1380
Fnu4HI GCNGC 4 cut(s) 678, 705, 972, 1025
FokI GGATG 1 cut(s) 1189
Fsp4HI GCNGC 4 cut(s) 678, 705, 972, 1025
FspBI CTAG 2 cut(s) 351, 516
GlaI GCGC 1 cut(s) 690
GluI GCNGC 4 cut(s) 678, 705, 972, 1025
HaeII RGCGCY 1 cut(s) 692
HaeIII GGCC 4 cut(s) 5, 104, 213, 1027
HapII CCGG 2 cut(s) 6, 1127
HhaI GCGC 1 cut(s) 691
Hin1I GRCGYC 1 cut(s) 689
Hin1II CATG 5 cut(s) 59, 98, 419, 909, 1384
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HindIII AAGCTT 2 cut(s) 138, 610
HinfI GANTC 4 cut(s) 434, 520, 1156, 1240
HpaII CCGG 2 cut(s) 6, 1127
HphI GGTGA 5 cut(s) 305, 661, 1052, 1174, 1444
Hpy188I TCNGA 4 cut(s) 250, 260, 400, 1374
Hpy188III TCNNGA 4 cut(s) 125, 931, 1329, 1424
HpyAV CCTTC 8 cut(s) 131, 156, 522, 664, 724, 829, 1061, 1430
HpyCH4III ACNGT 5 cut(s) 727, 937, 1149, 1319, 1454
HpyCH4IV ACGT 1 cut(s) 1180
HpyF10VI GCNNNNNNNGC 4 cut(s) 674, 686, 971, 1021
HpyF3I CTNAG 8 cut(s) 192, 208, 369, 524, 807, 998, 1185, 1297
HpySE526I ACGT 1 cut(s) 1180
Hsp92I GRCGYC 1 cut(s) 689
Hsp92II CATG 5 cut(s) 59, 98, 419, 909, 1384
HspAI GCGC 1 cut(s) 689
KasI GGCGCC 1 cut(s) 688
KpnI GGTACC 2 cut(s) 1139, 1470
Kzo9I GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
LguI GCTCTTC 1 cut(s) 1386
LmnI GCTCC 4 cut(s) 245, 620, 734, 1315
Lsp1109I GCAGC 3 cut(s) 664, 716, 958
LweI GCATC 4 cut(s) 178, 349, 501, 1211
MaeI CTAG 2 cut(s) 351, 516
MaeII ACGT 1 cut(s) 1180
MaeIII GTNAC 6 cut(s) 280, 364, 641, 649, 1176, 1319
MalI GATC 8 cut(s) 43, 155, 410, 633, 1060, 1211, 1223, 1439
MbiI CCGCTC 1 cut(s) 737
MboI GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
MboII GAAGA 6 cut(s) 210, 394, 602, 787, 1403, 1447
MflI RGATCY 3 cut(s) 631, 1058, 1209
MhlI GDGCHC 1 cut(s) 1186
MluCI AATT 7 cut(s) 33, 202, 672, 1016, 1121, 1141, 1480
Mly113I GGCGCC 1 cut(s) 689
MlyI GAGTC 2 cut(s) 428, 1165
MmeI TCCRAC 2 cut(s) 928, 1305
MnlI CCTC 9 cut(s) 63, 283, 423, 622, 944, 1254, 1269, 1408, 1420
MroXI GAANNNNTTC 2 cut(s) 594, 1122
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 3 cut(s) 186, 540, 754
MspI CCGG 2 cut(s) 6, 1127
MspR9I CCNGG 2 cut(s) 100, 1474
Mva1269I GAATGC 1 cut(s) 1117
MvaI CCWGG 2 cut(s) 100, 1474
MvnI CGCG 2 cut(s) 1024, 1330
MwoI GCNNNNNNNGC 4 cut(s) 674, 686, 971, 1021
NarI GGCGCC 1 cut(s) 689
NdeII GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
NlaIII CATG 5 cut(s) 59, 98, 419, 909, 1384
NlaIV GGNNCC 6 cut(s) 633, 690, 761, 1060, 1137, 1468
NmuCI GTSAC 2 cut(s) 649, 1176
NruI TCGCGA 1 cut(s) 1330
NspI RCATGY 1 cut(s) 909
OliI CACNNNNGTG 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 1386
PctI GAATGC 1 cut(s) 1117
PdmI GAANNNNTTC 2 cut(s) 594, 1122
PfeI GAWTC 2 cut(s) 520, 1240
Pfl23II CGTACG 1 cut(s) 222
PkrI GCNGC 4 cut(s) 679, 706, 973, 1026
PleI GAGTC 2 cut(s) 428, 1164
PluTI GGCGCC 1 cut(s) 692
PmaCI CACGTG 1 cut(s) 1181
PmlI CACGTG 1 cut(s) 1181
PpsI GAGTC 2 cut(s) 428, 1164
Ppu21I YACGTR 1 cut(s) 1181
PpuMI RGGWCCY 1 cut(s) 1417
PshAI GACNNNNGTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 1417
Psp6I CCWGG 2 cut(s) 98, 1472
PspCI CACGTG 1 cut(s) 1181
PspEI GGTNACC 1 cut(s) 649
PspGI CCWGG 2 cut(s) 98, 1472
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 6 cut(s) 633, 690, 761, 1060, 1137, 1468
PspPI GGNCC 4 cut(s) 102, 131, 1278, 1417
PspPPI RGGWCCY 1 cut(s) 1417
PstI CTGCAG 1 cut(s) 1221
PsuI RGATCY 3 cut(s) 631, 1058, 1209
RruI TCGCGA 1 cut(s) 1330
RsaI GTAC 4 cut(s) 224, 490, 1137, 1468
RsaNI GTAC 4 cut(s) 223, 489, 1136, 1467
RseI CAYNNNNRTG 3 cut(s) 186, 540, 754
SapI GCTCTTC 1 cut(s) 1386
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 4 cut(s) 678, 705, 972, 1025
Sau3AI GATC 8 cut(s) 41, 153, 408, 631, 1058, 1209, 1221, 1437
Sau96I GGNCC 4 cut(s) 102, 131, 1278, 1417
SchI GAGTC 2 cut(s) 428, 1165
ScrFI CCNGG 2 cut(s) 100, 1474
SduI GDGCHC 1 cut(s) 1186
SfaNI GCATC 4 cut(s) 178, 349, 501, 1211
SfcI CTRYAG 2 cut(s) 699, 1217
SfoI GGCGCC 1 cut(s) 690
SinI GGWCC 3 cut(s) 131, 1278, 1417
SmiMI CAYNNNNRTG 3 cut(s) 186, 540, 754
SmlI CTYRAG 2 cut(s) 26, 1424
SmoI CTYRAG 2 cut(s) 26, 1424
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 7 cut(s) 33, 202, 672, 1016, 1121, 1141, 1480
SsiI CCGC 6 cut(s) 114, 558, 737, 963, 1024, 1430
SspDI GGCGCC 1 cut(s) 688
SspMI CTAG 2 cut(s) 351, 516
StyD4I CCNGG 2 cut(s) 98, 1472
StyI CCWWGG 2 cut(s) 515, 985
TaaI ACNGT 5 cut(s) 727, 937, 1149, 1319, 1454
TaiI ACGT 1 cut(s) 1183
TaqI TCGA 5 cut(s) 149, 167, 357, 1154, 1224
TasI AATT 7 cut(s) 33, 202, 672, 1016, 1121, 1141, 1480
TauI GCSGC 1 cut(s) 1027
TfiI GAWTC 2 cut(s) 520, 1240
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 5 cut(s) 67, 1152, 1193, 1221, 1322
TseFI GTSAC 2 cut(s) 649, 1176
TseI GCWGC 3 cut(s) 677, 704, 971
Tsp45I GTSAC 2 cut(s) 649, 1176
TspDTI ATGAA 3 cut(s) 153, 646, 1272
TspGWI ACGGA 1 cut(s) 1051
TspRI CASTG 5 cut(s) 67, 1152, 1193, 1221, 1322
VpaK11BI GGWCC 3 cut(s) 131, 1278, 1417
XagI CCTNNNNNAGG 1 cut(s) 1424
XapI RAATTY 1 cut(s) 1121
XceI RCATGY 1 cut(s) 909
XmaJI CCTAGG 1 cut(s) 515
XmnI GAANNNNTTC 2 cut(s) 594, 1122
XspI CTAG 2 cut(s) 351, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.