Rw1G023620

Mechanosensitive ion channel protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005685
Physical Location & Seq
Forward (+)
49570095 .. 49574145
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023620.1

Sequence Viewer

Length: 1647 bp
ATGGCTGGACGTAGGCTTTCCATGTTGAAGTCATTTTGCAAGTCTACCAATTGCTCTCTCAACCCAATGTCGTTTCAGTTGTCTAGTTCTTATGGGAAATTTGTGAGCTATGCTGATTCAGTTCTTGTAAGACCATCGTATGCTTTTGTGAATCGCGGTTACCATACAAAGGAAACAGAATCCCATAAAACTTTTGCAAATACTCAGATTAAAGCACTGGGTTCTGGATCACTTGTTAATACACGATGTTACGGAACCAAACCTAGTACAGCATTTTCACCCACATTGTCAAGTTCACTTAGTAGGTGTGCTTTTCCTCCTGTTTCGATAAGCCCAATTCTGGGTTGCCGTTCGTACTCATCATTTTTCGGTAGCAATGCTGATAAAGATGGGCAACCTGGAGTTCCGGCTGCTTCCAGTGGCAGTGAAGTGGATGTTGGTAATAGTGGCGCTGGTGGAAGTGATTTGGTTGATAAGGTTAAGGAATTCGTGCAGGGTGCTGCGGATGCGGTAACTCATACTGGGCAGAAGGCTAAAGAAGCATCTAGTGAAATTTCCCCTTATGTTCAGCAATTGTTGGATGCATATCCACATCTTAGAAATGTGGTTACTCCAGTTGGTTGTACTTTGGCAGGTACTATATTTGCTTGGCTAGTGATGCCTAGGCTTTTCAGGAGGTTTCACAAGTACGCAAGTCTAGGCCGCACTGCTTTACTATCTGGAAGCCTTGAGGATCAAGTTCCATATGAGAAAAGTTTTTGGGGTGCTTTGGAGGATCCAGTGCGATACCTATTTACTTTTATGGCATTTTCACAGATTGGCACAATGGTTGCACCAACCGCTATAGCTGCTCAATATCTTGCACCAGCTTGGAGAGGTGCAGCTATCATTTCATTTGTTTGGTTTCTATATCGGTGGAAGACAAATGTGTATACTCGCGCATTAGCAACTAAAAGTTTGCCCAGCAGTCTTGATCGGGAGAAGTTGTTAGCTCTAGACAAAATTTCATCTGTTGGTCTTTTTATGATTGGGATAATGGCTCTAGCTGAGGCATGTGGGGTGGCTGTGCAATCTATTATGACTGTTGGTGGCATAGGAGGAGTTGCTACTGCTTTTGCTGCCAGAGATGTCCTTGGCAATGTGCTGAGTGGTTTATCTATGCAATTTACAAAGCCCTTTTCACTAGGTGATACAATAAAGGCTGGGTCCATAGAAGGTCAAGTGGTGGATATGGGACTTACAACCACGTCATTACTGAATGCCGAGAAGTTCCCAGTGATAGTTCCCAATTCCCTCTTTTCTAGTCAGGTGATTGTGAACAAGTCACGTGCTCAATGGCGTGGTGTCGTCACGAGAATTCCCCTGCAAATCAATGATGATTTGGATAAGGTTCCCCAGATATCAGATGATATAAAAAGCATGCTTAGATCACACCCAAAGGTTTTCTTGGGAAAGGAGGTGCCTTATTGTTTCCTTTCTCGTCTAGAGAGCTCATTTGGAGAATTGACTCTTGGATGTAACCTCAAACAGATGAGCAAGGATGAGTTATTCTCTACAGAAGAAGATATCCTTTTGCGGTCGGTCAAGATAATTAAGGGGCATGGCGCCAAGTTGGGCAGCACCTATGGTGATATGACCACTACATAG

Protein Analysis

548

Amino Acids

59.15

Weight (kDa)

9.2

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 376 - 443 3.8e-16 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 2 cut(s) 1459, 1604
AccI GTMKAC 2 cut(s) 44, 932
AccII CGCG 2 cut(s) 156, 939
AciI CCGC 6 cut(s) 156, 503, 509, 703, 840, 1576
AclWI GGATC 4 cut(s) 235, 741, 770, 783
AcsI RAATTY 5 cut(s) 98, 485, 552, 1002, 1356
AcvI CACGTG 1 cut(s) 1328
AcyI GRCGYC 1 cut(s) 1605
AdeI CACNNNGTG 1 cut(s) 1187
AfaI GTAC 5 cut(s) 268, 356, 625, 637, 689
AfiI CCNNNNNNNGG 3 cut(s) 169, 340, 341
AgsI TTSAA 1 cut(s) 28
AjiI CACGTC 1 cut(s) 1248
AjnI CCWGG 1 cut(s) 397
AjuI GAANNNNNNNTTGG 4 cut(s) 420, 452, 1494, 1526
AluBI AGCT 7 cut(s) 108, 848, 869, 884, 992, 1046, 1491
AluI AGCT 7 cut(s) 108, 848, 869, 884, 992, 1046, 1491
Alw21I GWGCWC 2 cut(s) 1333, 1493
AlwI GGATC 4 cut(s) 235, 741, 770, 783
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 6 cut(s) 410, 500, 848, 881, 1118, 1617
ApoI RAATTY 5 cut(s) 98, 485, 552, 1002, 1356
AspA2I CCTAGG 1 cut(s) 662
AspLEI GCGC 3 cut(s) 452, 941, 1607
AspS9I GGNCC 1 cut(s) 1206
AsuHPI GGTGA 4 cut(s) 270, 1199, 1321, 1640
AvaII GGWCC 1 cut(s) 1206
AvrII CCTAGG 1 cut(s) 662
BamHI GGATCC 1 cut(s) 775
BanI GGYRCC 2 cut(s) 1459, 1604
BanII GRGCYC 1 cut(s) 1493
BauI CACGAG 1 cut(s) 1351
BbrPI CACGTG 1 cut(s) 1328
BbsI GAAGAC 1 cut(s) 926
Bbv12I GWGCWC 2 cut(s) 1333, 1493
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 6 cut(s) 397, 487, 835, 893, 1105, 1629
BccI CCATC 2 cut(s) 142, 383
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 399
BfmI CTRYAG 2 cut(s) 843, 1554
BfoI RGCGCY 2 cut(s) 453, 1608
BfuAI ACCTGC 1 cut(s) 623
BisI GCNGC 7 cut(s) 411, 501, 703, 849, 882, 1119, 1618
BlnI CCTAGG 1 cut(s) 662
BlsI GCNGC 7 cut(s) 412, 502, 704, 850, 883, 1120, 1619
Bme1390I CCNGG 1 cut(s) 399
Bme18I GGWCC 1 cut(s) 1206
BmgBI CACGTC 1 cut(s) 1248
BmgT120I GGNCC 1 cut(s) 1206
BmiI GGNNCC 6 cut(s) 256, 777, 1207, 1392, 1461, 1606
BmrFI CCNGG 1 cut(s) 399
BmrI ACTGGG 3 cut(s) 227, 531, 1268
BmsI GCATC 4 cut(s) 496, 551, 571, 648
BmuI ACTGGG 3 cut(s) 227, 531, 1268
BpiI GAAGAC 1 cut(s) 926
BplI GAGNNNNNCTC 2 cut(s) 1535, 1567
BpmI CTGGAG 2 cut(s) 420, 597
Bpu10I CCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 1 cut(s) 749
BsaAI YACGTR 1 cut(s) 1328
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 1605
BsaJI CCNNGG 2 cut(s) 662, 1132
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 340, 341
Bse1I ACTGG 6 cut(s) 222, 417, 526, 614, 779, 1274
Bse3DI GCAATG 2 cut(s) 382, 1144
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 662, 1132
BseGI GGATG 5 cut(s) 439, 511, 586, 1520, 1546
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 169, 340, 341
BseMI GCAATG 2 cut(s) 382, 1144
BseMII CTCAG 3 cut(s) 218, 1038, 1136
BseNI ACTGG 6 cut(s) 222, 417, 526, 614, 779, 1274
BseRI GAGGAG 1 cut(s) 1113
BseXI GCAGC 6 cut(s) 397, 487, 835, 893, 1105, 1629
BseYI CCCAGC 2 cut(s) 962, 1202
BsgI GTGCAG 2 cut(s) 512, 900
Bsh1236I CGCG 2 cut(s) 156, 939
Bsh1285I CGRYCG 1 cut(s) 1580
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 2 cut(s) 1459, 1604
BsiEI CGRYCG 1 cut(s) 1580
BsiHKAI GWGCWC 2 cut(s) 1333, 1493
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 1248
BslI CCNNNNNNNGG 3 cut(s) 169, 340, 341
BsmFI GGGAC 1 cut(s) 1248
BsmI GAATGC 1 cut(s) 1264
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 1333, 1493
Bsp143I GATC 5 cut(s) 227, 733, 775, 973, 1427
BspACI CCGC 6 cut(s) 156, 503, 509, 703, 840, 1576
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 3 cut(s) 217, 1039, 1137
BspFNI CGCG 2 cut(s) 156, 939
BspLI GGNNCC 6 cut(s) 256, 777, 1207, 1392, 1461, 1606
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 4 cut(s) 235, 741, 770, 783
BspT107I GGYRCC 2 cut(s) 1459, 1604
BsrDI GCAATG 2 cut(s) 382, 1144
BsrI ACTGG 6 cut(s) 222, 417, 526, 614, 779, 1274
BssECI CCNNGG 2 cut(s) 662, 1132
BssMI GATC 5 cut(s) 227, 733, 775, 973, 1427
BssNAI GTATAC 1 cut(s) 933
BssNI GRCGYC 1 cut(s) 1605
BssSI CACGAG 1 cut(s) 1351
BssT1I CCWWGG 2 cut(s) 662, 1132
Bst1107I GTATAC 1 cut(s) 933
Bst2BI CACGAG 1 cut(s) 1351
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 1084
BstACI GRCGYC 1 cut(s) 1605
BstBAI YACGTR 1 cut(s) 1328
BstC8I GCNNGC 1 cut(s) 1421
BstDEI CTNAG 6 cut(s) 204, 299, 596, 1047, 1145, 1424
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 5 cut(s) 439, 511, 586, 1520, 1546
BstFNI CGCG 2 cut(s) 156, 939
BstH2I RGCGCY 2 cut(s) 453, 1608
BstHHI GCGC 3 cut(s) 452, 941, 1607
BstKTI GATC 5 cut(s) 230, 736, 778, 976, 1430
BstMBI GATC 5 cut(s) 227, 733, 775, 973, 1427
BstMCI CGRYCG 1 cut(s) 1580
BstMWI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 2 cut(s) 1056, 1423
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 2 cut(s) 843, 1554
BstUI CGCG 2 cut(s) 156, 939
BstV1I GCAGC 6 cut(s) 397, 487, 835, 893, 1105, 1629
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BstZ17I GTATAC 1 cut(s) 933
BsuRI GGCC 1 cut(s) 702
BtrI CACGTC 1 cut(s) 1248
BtsCI GGATG 5 cut(s) 439, 511, 586, 1520, 1546
BtsI GCAGTG 2 cut(s) 430, 705
BtsIMutI CAGTG 6 cut(s) 215, 424, 430, 705, 786, 1281
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 1 cut(s) 1421
CfoI GCGC 3 cut(s) 452, 941, 1607
Cfr13I GGNCC 1 cut(s) 1206
Csp6I GTAC 5 cut(s) 267, 355, 624, 636, 688
CviAII CATG 4 cut(s) 22, 1053, 1420, 1601
CviQI GTAC 5 cut(s) 267, 355, 624, 636, 688
DdeI CTNAG 6 cut(s) 204, 299, 596, 1047, 1145, 1424
DinI GGCGCC 1 cut(s) 1606
DpnI GATC 5 cut(s) 229, 735, 777, 975, 1429
DpnII GATC 5 cut(s) 227, 733, 775, 973, 1427
DraIII CACNNNGTG 1 cut(s) 1187
Ecl136II GAGCTC 1 cut(s) 1491
Eco130I CCWWGG 2 cut(s) 662, 1132
Eco24I GRGCYC 1 cut(s) 1493
Eco32I GATATC 2 cut(s) 1401, 1567
Eco47I GGWCC 1 cut(s) 1206
Eco53kI GAGCTC 1 cut(s) 1491
Eco72I CACGTG 1 cut(s) 1328
Eco91I GGTNACC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 1491
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 2 cut(s) 485, 1356
EcoRII CCWGG 1 cut(s) 397
EcoRV GATATC 2 cut(s) 1401, 1567
EcoT14I CCWWGG 2 cut(s) 662, 1132
EcoT22I ATGCAT 1 cut(s) 586
EcoT38I GRGCYC 1 cut(s) 1493
EgeI GGCGCC 1 cut(s) 1606
EheI GGCGCC 1 cut(s) 1606
ErhI CCWWGG 2 cut(s) 662, 1132
FaeI CATG 4 cut(s) 25, 1056, 1423, 1604
FalI AAGNNNNNCTT 4 cut(s) 1430, 1462, 1554, 1586
FaqI GGGAC 1 cut(s) 1248
FatI CATG 4 cut(s) 21, 1052, 1419, 1600
FauNDI CATATG 1 cut(s) 745
FblI GTMKAC 2 cut(s) 44, 932
Fnu4HI GCNGC 7 cut(s) 411, 501, 703, 849, 882, 1119, 1618
FokI GGATG 5 cut(s) 446, 518, 593, 1527, 1553
FriOI GRGCYC 1 cut(s) 1493
Fsp4HI GCNGC 7 cut(s) 411, 501, 703, 849, 882, 1119, 1618
GlaI GCGC 3 cut(s) 451, 940, 1606
GluI GCNGC 7 cut(s) 411, 501, 703, 849, 882, 1119, 1618
GsaI CCCAGC 2 cut(s) 966, 1206
GsuI CTGGAG 2 cut(s) 420, 597
HaeII RGCGCY 2 cut(s) 453, 1608
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 407
HhaI GCGC 3 cut(s) 452, 941, 1607
Hin1I GRCGYC 1 cut(s) 1605
Hin1II CATG 4 cut(s) 25, 1056, 1423, 1604
Hin6I GCGC 3 cut(s) 450, 939, 1605
HinP1I GCGC 3 cut(s) 450, 939, 1605
HinfI GANTC 4 cut(s) 116, 151, 179, 1507
HpaII CCGG 1 cut(s) 407
HphI GGTGA 4 cut(s) 270, 1199, 1321, 1640
Hpy166II GTNNAC 4 cut(s) 45, 296, 933, 1318
Hpy188I TCNGA 2 cut(s) 207, 1405
Hpy188III TCNNGA 9 cut(s) 225, 673, 720, 971, 977, 995, 1351, 1484, 1585
Hpy8I GTNNAC 4 cut(s) 45, 296, 933, 1318
HpyAV CCTTC 2 cut(s) 523, 1208
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 3 cut(s) 10, 1247, 1327
HpyF10VI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
HpyF3I CTNAG 6 cut(s) 204, 299, 596, 1047, 1145, 1424
HpySE526I ACGT 3 cut(s) 10, 1247, 1327
Hsp92I GRCGYC 1 cut(s) 1605
Hsp92II CATG 4 cut(s) 25, 1056, 1423, 1604
HspAI GCGC 3 cut(s) 450, 939, 1605
KasI GGCGCC 1 cut(s) 1604
Kzo9I GATC 5 cut(s) 227, 733, 775, 973, 1427
Lsp1109I GCAGC 6 cut(s) 397, 487, 835, 893, 1105, 1629
LweI GCATC 4 cut(s) 496, 551, 571, 648
MaeII ACGT 3 cut(s) 10, 1247, 1327
MaeIII GTNAC 7 cut(s) 158, 248, 511, 607, 1323, 1348, 1517
MalI GATC 5 cut(s) 229, 735, 777, 975, 1429
MboI GATC 5 cut(s) 227, 733, 775, 973, 1427
MboII GAAGA 3 cut(s) 931, 1571, 1574
MfeI CAATTG 2 cut(s) 49, 572
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 2 cut(s) 1333, 1493
Mly113I GGCGCC 1 cut(s) 1605
MlyI GAGTC 1 cut(s) 1501
MmeI TCCRAC 1 cut(s) 558
Mph1103I ATGCAT 1 cut(s) 586
MseI TTAA 4 cut(s) 210, 237, 480, 1593
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 2 cut(s) 49, 572
Mva1269I GAATGC 1 cut(s) 1264
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 2 cut(s) 156, 939
MwoI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
NarI GGCGCC 1 cut(s) 1605
NdeI CATATG 1 cut(s) 745
NdeII GATC 5 cut(s) 227, 733, 775, 973, 1427
NlaIII CATG 4 cut(s) 25, 1056, 1423, 1604
NlaIV GGNNCC 6 cut(s) 256, 777, 1207, 1392, 1461, 1606
NmeAIII GCCGAG 1 cut(s) 1288
NmuCI GTSAC 2 cut(s) 1323, 1348
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 2 cut(s) 1056, 1423
PaeI GCATGC 1 cut(s) 1423
PctI GAATGC 1 cut(s) 1264
PfeI GAWTC 3 cut(s) 116, 151, 179
PkrI GCNGC 7 cut(s) 412, 502, 704, 850, 883, 1120, 1619
PleI GAGTC 1 cut(s) 1501
PluTI GGCGCC 1 cut(s) 1608
PmaCI CACGTG 1 cut(s) 1328
PmlI CACGTG 1 cut(s) 1328
PpsI GAGTC 1 cut(s) 1501
Ppu21I YACGTR 1 cut(s) 1328
Psp124BI GAGCTC 1 cut(s) 1493
Psp6I CCWGG 1 cut(s) 397
PspCI CACGTG 1 cut(s) 1328
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 2 cut(s) 962, 1202
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 6 cut(s) 256, 777, 1207, 1392, 1461, 1606
PspPI GGNCC 1 cut(s) 1206
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 5 cut(s) 268, 356, 625, 637, 689
RsaNI GTAC 5 cut(s) 267, 355, 624, 636, 688
SacI GAGCTC 1 cut(s) 1493
SaqAI TTAA 4 cut(s) 210, 237, 480, 1593
SatI GCNGC 7 cut(s) 411, 501, 703, 849, 882, 1119, 1618
Sau3AI GATC 5 cut(s) 227, 733, 775, 973, 1427
Sau96I GGNCC 1 cut(s) 1206
SchI GAGTC 1 cut(s) 1501
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 2 cut(s) 1333, 1493
SfaNI GCATC 4 cut(s) 496, 551, 571, 648
SfcI CTRYAG 2 cut(s) 843, 1554
SfoI GGCGCC 1 cut(s) 1606
SinI GGWCC 1 cut(s) 1206
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SphI GCATGC 1 cut(s) 1423
SsiI CCGC 6 cut(s) 156, 503, 509, 703, 840, 1576
SspDI GGCGCC 1 cut(s) 1604
SstI GAGCTC 1 cut(s) 1493
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 2 cut(s) 662, 1132
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 3 cut(s) 13, 1250, 1330
TaqI TCGA 1 cut(s) 326
TaqII GACCGA 1 cut(s) 1570
TatI WGTACW 2 cut(s) 266, 623
TauI GCSGC 1 cut(s) 705
TfiI GAWTC 3 cut(s) 116, 151, 179
Tru1I TTAA 4 cut(s) 210, 237, 480, 1593
Tru9I TTAA 4 cut(s) 210, 237, 480, 1593
TscAI CASTG 6 cut(s) 222, 424, 430, 712, 786, 1281
TseFI GTSAC 2 cut(s) 1323, 1348
TseI GCWGC 6 cut(s) 410, 500, 848, 881, 1118, 1617
Tsp45I GTSAC 2 cut(s) 1323, 1348
TspDTI ATGAA 2 cut(s) 882, 996
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 6 cut(s) 222, 424, 430, 712, 786, 1281
VpaK11BI GGWCC 1 cut(s) 1206
XapI RAATTY 5 cut(s) 98, 485, 552, 1002, 1356
XbaI TCTAGA 2 cut(s) 994, 1483
XceI RCATGY 2 cut(s) 1056, 1423
XmaJI CCTAGG 1 cut(s) 662
XmiI GTMKAC 2 cut(s) 44, 932
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.