Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0005685

Physical Location & Seq

Genomic Coordinates Reverse (-)

14545894 .. 14548883

Gene Structure 2990 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g13030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGCTGGAGTTAGGCTTTCCATGTTGAAATCACTTCGCAATTCTACCCCTTGCTCTCTGAACTCGCAGTCGTTTCAGTCGTATACTTCTTATTGGAAAATTGTGAACTACACTGATCCGGGTCATATAAGACCAGCCTATGCTTTTGTAAATCGGAGTTACCATTCGAAGGAAACACAATCTCATAACAACTTTGCAAAGGCTCATTCTAGAGCATTGGGTTCTGAATCATTTATCAATATCCAATCTTACAGTACGAAACCCATTTCAGCATTTTCATCTACATTGTCGAGTACACAGATTAGGGGTGCTACTCCACTGGCTTTGATAAACCCAATTTTTAGTAGCCGTTCATACTCATCATTTTTCGGTAGCAATGCTGATAAAACTGGGGAACCAGGACTAGGAGTTCCGGCTGCTTCTGGTGGAAGTGAAGTGGATGTTGGTAATAGTGGCATTGTTGGAAGTGACTGGGTTGGCAAGTTTAAGGATGCATGGCAGAGTGCATTGGATGCGGCAACTTTTACCGGACAGAAGGCTAAAGAAACCTCCACTGAACTGTCTCCTTATATTCAGCAGTTGCTGGATTCAAATCCACATCTTAGAAATGTGGTTGTTCCTGTTGGTTGTACTTTGGCAGGTACTATATTTGCTTGGTTTGTGATGCCGAGGCTTTTCAGGAGATTTCACAATTACGGAAATCAAGGGCGTGCTGCTTTGCTATCTGCAAGTCCATCTGAAGAGCAAGTTCCATATGAGAAAAGTTTTTGGGGTGCTTTGGAGGACCCAGTGCGATATCTAGTTACGTTCATGGCATTTTCTCAAATTGGTGTGATGGTAGCACCAACCACTATAGCTGCACAGTATCTTGCACCAGCATGGAGAGGTGCAGTTATCCTTTCATTTGTGTGGTTTCTACATCGGTGGAAGACAAACGTGTTCACTCGTGCATTAGAAACTCAAACTTTGCCTGGTCTTGATCGGGACAAGTTGCTAGCTATGGACAACATTTCATCCGTTGGTCTTTTTGTGATTGGGATAATGGCTTTAGCTGAGGCATGTGGGGTGGCTGTACAATCAATTTTGACAGTTGGTGGCATAGGAGGAGTTGCTACTGCATTTGCTGCCAGAGACATCCTTGGAAATGTTCTGAGTGGGTTGTCTATGCAGTTTTCAAAGCCGTTTTCACTTGGAGATACTATAAAAGCTGGATCTATTGAAGGTCAAGTGGTGGAAATGGGACTTACTACTACTTGTTTACTGAATGCCGAGAAGTTCCCTGTCATAGTTCCTAATTCACTCTTTTCTAGTCAGGTAATTGTGAACAAGTCACGTGCGCAATGGCGTGCCATTGTCACCAAAATTCCCCTGCAAATTAATGATTTGGATAAGATTCCCCAGATATCAACTGATATAAAGAGCATGCTAAGGTCACACTCGAAGGTGTTCTTGGGAAAGGAGGCCCCTTACTGTTTCCTGTCTCGTTTAGAAAGTTCATTTGCTGAATTGACTCTCGGATGTAACCTCAAACATATGAGCAAAGGAGAGCTATACTCTACTGAAGAAGAGATTATCCTGCGCTCGGCTCAGATAATTAAGGAGCATGGTGCTAAGCTGGGCAGCACCTACCAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006950 GO:0006979 GO:0008150 GO:0008381 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0015267 GO:0016020 GO:0019866 GO:0022803 GO:0022836 GO:0022857 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0033554 GO:0034599 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0070887

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

546

Amino Acids

59.33

Weight (kDa)

9.18

Isoelectric Point (pI)

41.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	378 - 445	2.8e-16	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00234 AT4G00290
fragaria_vesca	FvH4_7g15010 FvH4_7g15010 FvH4_7g15010 FvH4_7g15010 FvH4_7g15010 FvH4_7g15010
malus_domestica	MD01G1068400.v1.1
prunus_persica	Prupe.2G174600_v2.0.a1 Prupe.2G174600_v2.0.a1
pyrus_communis	pycom07g13030
rosa_chinensis	RchiOBHm_Chr1g0357041
rosa_laevigata	RLG00000028058
rosa_multiflora	Rmu_sc0000166.1_g000040 Rmu_sc0013980.1_g000024
rosa_roxburghii	Rroxscaffold_4G00299100 Rroxscaffold_6G00397860 Rroxscaffold_7G00208020
rosa_rugosa	Rorug01G0253800
rosa_samantha	Rh1AG267500 Rh1BG235300 Rh1BG235400 Rh1CG146800 Rh1CG251500 Rh1DG262700 Rh1DG262800
rosa_wichuraiana	Rw1G023620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1338
Acc36I	ACCTGC	1 cut(s)	629
AccI	GTMKAC	1 cut(s)	83
AciI	CCGC	1 cut(s)	515
AclWI	GGATC	2 cut(s)	110, 1219
AcsI	RAATTY	1 cut(s)	1362
AcuI	CTGAAG	2 cut(s)	759, 1581
AcvI	CACGTG	1 cut(s)	1334
AfaI	GTAC	5 cut(s)	256, 295, 631, 643, 1074
AfiI	CCNNNNNNNGG	3 cut(s)	169, 404, 1582
AflIII	ACRYGT	1 cut(s)	936
AgsI	TTSAA	4 cut(s)	28, 591, 1176, 1220
AjnI	CCWGG	2 cut(s)	397, 970
AjuI	GAANNNNNNNTTGG	4 cut(s)	426, 458, 1433, 1465
AloI	GAACNNNNNNTCC	2 cut(s)	393, 425
AluBI	AGCT	6 cut(s)	857, 998, 1052, 1208, 1549, 1615
AluI	AGCT	6 cut(s)	857, 998, 1052, 1208, 1549, 1615
Alw26I	GTCTC	3 cut(s)	567, 1125, 1485
AlwI	GGATC	2 cut(s)	110, 1219
AlwNI	CAGNNNCTG	1 cut(s)	583
AoxI	GGCC	1 cut(s)	1461
ApeKI	GCWGC	5 cut(s)	416, 713, 857, 1124, 1620
ApoI	RAATTY	1 cut(s)	1362
AseI	ATTAAT	1 cut(s)	1377
Asp700I	GAANNNNTTC	1 cut(s)	1445
AspLEI	GCGC	2 cut(s)	1339, 1581
AspS9I	GGNCC	2 cut(s)	784, 1462
AsuC2I	CCSGG	1 cut(s)	120
AsuHPI	GGTGA	1 cut(s)	1348
AsuII	TTCGAA	1 cut(s)	167
AsuNHI	GCTAGC	1 cut(s)	994
AvaII	GGWCC	1 cut(s)	784
BauI	CACGAG	1 cut(s)	945
BbrPI	CACGTG	1 cut(s)	1334
BbsI	GAAGAC	1 cut(s)	935
BbvCI	CCTCAGC	1 cut(s)	1053
BbvI	GCAGC	5 cut(s)	403, 700, 844, 1111, 1632
BccI	CCATC	2 cut(s)	742, 829
BceAI	ACGGC	2 cut(s)	333, 1165
BciT130I	CCWGG	2 cut(s)	399, 972
BcnI	CCSGG	1 cut(s)	120
BcoDI	GTCTC	3 cut(s)	567, 1125, 1485
BfaI	CTAG	5 cut(s)	210, 404, 800, 995, 1308
BfmI	CTRYAG	1 cut(s)	852
BfuAI	ACCTGC	1 cut(s)	629
BisI	GCNGC	6 cut(s)	417, 516, 714, 858, 1125, 1621
BlpI	GCTNAGC	1 cut(s)	1611
BlsI	GCNGC	6 cut(s)	418, 517, 715, 859, 1126, 1622
Bme1390I	CCNGG	3 cut(s)	120, 399, 972
Bme18I	GGWCC	1 cut(s)	784
BmgT120I	GGNCC	2 cut(s)	784, 1462
BmiI	GGNNCC	3 cut(s)	396, 786, 1464
BmrFI	CCNGG	3 cut(s)	120, 399, 972
BmrI	ACTGGG	3 cut(s)	399, 481, 782
BmsI	GCATC	3 cut(s)	481, 502, 654
BmtI	GCTAGC	1 cut(s)	998
BmuI	ACTGGG	3 cut(s)	399, 481, 782
BpiI	GAAGAC	1 cut(s)	935
BplI	GAGNNNNNCTC	2 cut(s)	1538, 1570
BpmI	CTGGAG	1 cut(s)	27
Bpu10I	CCTNAGC	2 cut(s)	1053, 1427
Bpu1102I	GCTNAGC	1 cut(s)	1611
Bpu14I	TTCGAA	1 cut(s)	167
BpuMI	CCSGG	1 cut(s)	120
BsaAI	YACGTR	1 cut(s)	1334
BsaBI	GATNNNNATC	1 cut(s)	591
BsaJI	CCNNGG	2 cut(s)	668, 1138
BsaWI	WCCGGW	1 cut(s)	527
Bsc4I	CCNNNNNNNGG	3 cut(s)	169, 404, 1582
Bse1I	ACTGG	4 cut(s)	324, 394, 476, 788
Bse3DI	GCAATG	2 cut(s)	382, 1346
Bse8I	GATNNNNATC	1 cut(s)	591
BseBI	CCWGG	2 cut(s)	399, 972
BseDI	CCNNGG	2 cut(s)	668, 1138
BseGI	GGATG	6 cut(s)	445, 496, 517, 1013, 1134, 1523
BseJI	GATNNNNATC	1 cut(s)	591
BseLI	CCNNNNNNNGG	3 cut(s)	169, 404, 1582
BseMI	GCAATG	2 cut(s)	382, 1346
BseMII	CTCAG	3 cut(s)	1044, 1142, 1601
BseNI	ACTGG	4 cut(s)	324, 394, 476, 788
BseRI	GAGGAG	1 cut(s)	1119
BseXI	GCAGC	5 cut(s)	403, 700, 844, 1111, 1632
BseYI	CCCAGC	1 cut(s)	1615
BsgI	GTGCAG	2 cut(s)	843, 909
BshFI	GGCC	1 cut(s)	1463
BsiSI	CCGG	3 cut(s)	119, 413, 528
BslFI	GGGAC	2 cut(s)	998, 1254
BslI	CCNNNNNNNGG	3 cut(s)	169, 404, 1582
BsmAI	GTCTC	3 cut(s)	567, 1125, 1485
BsmFI	GGGAC	2 cut(s)	998, 1254
BsmI	GAATGC	1 cut(s)	1270
BsnI	GGCC	1 cut(s)	1463
Bsp119I	TTCGAA	1 cut(s)	167
Bsp1407I	TGTACA	1 cut(s)	1072
Bsp143I	GATC	3 cut(s)	115, 979, 1211
Bsp1720I	GCTNAGC	1 cut(s)	1611
BspACI	CCGC	1 cut(s)	515
BspANI	GGCC	1 cut(s)	1463
BspCNI	CTCAG	3 cut(s)	1045, 1143, 1600
BspLI	GGNNCC	3 cut(s)	396, 786, 1464
BspMI	ACCTGC	1 cut(s)	629
BspOI	GCTAGC	1 cut(s)	998
BspPI	GGATC	2 cut(s)	110, 1219
BspQI	GCTCTTC	1 cut(s)	735
BspT104I	TTCGAA	1 cut(s)	167
BsrDI	GCAATG	2 cut(s)	382, 1346
BsrGI	TGTACA	1 cut(s)	1072
BsrI	ACTGG	4 cut(s)	324, 394, 476, 788
BssECI	CCNNGG	2 cut(s)	668, 1138
BssMI	GATC	3 cut(s)	115, 979, 1211
BssNAI	GTATAC	1 cut(s)	84
BssSI	CACGAG	1 cut(s)	945
BssT1I	CCWWGG	1 cut(s)	1138
Bst1107I	GTATAC	1 cut(s)	84
Bst2BI	CACGAG	1 cut(s)	945
Bst2UI	CCWGG	2 cut(s)	399, 972
Bst4CI	ACNGT	5 cut(s)	254, 561, 864, 1090, 1472
Bst6I	CTCTTC	2 cut(s)	735, 1560
BstAPI	GCANNNNNTGC	2 cut(s)	512, 1124
BstAUI	TGTACA	1 cut(s)	1072
BstBAI	YACGTR	1 cut(s)	1334
BstBI	TTCGAA	1 cut(s)	167
BstC8I	GCNNGC	4 cut(s)	711, 996, 1347, 1424
BstDEI	CTNAG	6 cut(s)	602, 1053, 1151, 1427, 1587, 1611
BstF5I	GGATG	6 cut(s)	445, 496, 517, 1013, 1134, 1523
BstHHI	GCGC	2 cut(s)	1339, 1581
BstKTI	GATC	3 cut(s)	118, 982, 1214
BstMAI	GTCTC	3 cut(s)	567, 1125, 1485
BstMBI	GATC	3 cut(s)	115, 979, 1211
BstMWI	GCNNNNNNNGC	2 cut(s)	512, 1124
BstNI	CCWGG	2 cut(s)	399, 972
BstNSI	RCATGY	2 cut(s)	1062, 1426
BstSCI	CCNGG	3 cut(s)	118, 397, 970
BstSFI	CTRYAG	1 cut(s)	852
BstV1I	GCAGC	5 cut(s)	403, 700, 844, 1111, 1632
BstV2I	GAAGAC	1 cut(s)	935
BstX2I	RGATCY	1 cut(s)	1211
BstYI	RGATCY	1 cut(s)	1211
BstZ17I	GTATAC	1 cut(s)	84
BsuRI	GGCC	1 cut(s)	1463
BtsCI	GGATG	6 cut(s)	445, 496, 517, 1013, 1134, 1523
BtsIMutI	CAGTG	4 cut(s)	111, 317, 552, 795
BveI	ACCTGC	1 cut(s)	629
Cac8I	GCNNGC	4 cut(s)	711, 996, 1347, 1424
CaiI	CAGNNNCTG	1 cut(s)	583
CfoI	GCGC	2 cut(s)	1339, 1581
Cfr13I	GGNCC	2 cut(s)	784, 1462
Csp6I	GTAC	5 cut(s)	255, 294, 630, 642, 1073
CspCI	CAANNNNNGTGG	2 cut(s)	306, 341
CviAII	CATG	7 cut(s)	22, 495, 811, 879, 1059, 1423, 1604
CviQI	GTAC	5 cut(s)	255, 294, 630, 642, 1073
DdeI	CTNAG	6 cut(s)	602, 1053, 1151, 1427, 1587, 1611
DpnI	GATC	3 cut(s)	117, 981, 1213
DpnII	GATC	3 cut(s)	115, 979, 1211
Eam1104I	CTCTTC	2 cut(s)	735, 1560
EarI	CTCTTC	2 cut(s)	735, 1560
Eco130I	CCWWGG	1 cut(s)	1138
Eco32I	GATATC	2 cut(s)	797, 1404
Eco47I	GGWCC	1 cut(s)	784
Eco57I	CTGAAG	2 cut(s)	759, 1581
Eco72I	CACGTG	1 cut(s)	1334
EcoO109I	RGGNCCY	2 cut(s)	784, 1462
EcoRII	CCWGG	2 cut(s)	397, 970
EcoRV	GATATC	2 cut(s)	797, 1404
EcoT14I	CCWWGG	1 cut(s)	1138
EcoT22I	ATGCAT	1 cut(s)	496
ErhI	CCWWGG	1 cut(s)	1138
FaeI	CATG	7 cut(s)	25, 498, 814, 882, 1062, 1426, 1607
FalI	AAGNNNNNCTT	2 cut(s)	1433, 1465
FaqI	GGGAC	2 cut(s)	998, 1254
FatI	CATG	7 cut(s)	21, 494, 810, 878, 1058, 1422, 1603
FauNDI	CATATG	2 cut(s)	754, 1533
FblI	GTMKAC	1 cut(s)	83
Fnu4HI	GCNGC	6 cut(s)	417, 516, 714, 858, 1125, 1621
FokI	GGATG	6 cut(s)	452, 503, 524, 1000, 1121, 1530
Fsp4HI	GCNGC	6 cut(s)	417, 516, 714, 858, 1125, 1621
FspBI	CTAG	5 cut(s)	210, 404, 800, 995, 1308
FspI	TGCGCA	1 cut(s)	1338
GlaI	GCGC	2 cut(s)	1338, 1580
GluI	GCNGC	6 cut(s)	417, 516, 714, 858, 1125, 1621
GsaI	CCCAGC	1 cut(s)	1619
GsuI	CTGGAG	1 cut(s)	27
HaeIII	GGCC	1 cut(s)	1463
HapII	CCGG	3 cut(s)	119, 413, 528
HhaI	GCGC	2 cut(s)	1339, 1581
Hin1II	CATG	7 cut(s)	25, 498, 814, 882, 1062, 1426, 1607
Hin6I	GCGC	2 cut(s)	1337, 1579
HinP1I	GCGC	2 cut(s)	1337, 1579
HinfI	GANTC	4 cut(s)	227, 587, 1393, 1510
HpaII	CCGG	3 cut(s)	119, 413, 528
HphI	GGTGA	1 cut(s)	1348
Hpy166II	GTNNAC	6 cut(s)	84, 106, 296, 942, 1259, 1324
Hpy188I	TCNGA	7 cut(s)	60, 156, 226, 739, 1152, 1517, 1590
Hpy188III	TCNNGA	4 cut(s)	210, 679, 977, 983
Hpy8I	GTNNAC	6 cut(s)	84, 106, 296, 942, 1259, 1324
HpyAV	CCTTC	4 cut(s)	163, 529, 1214, 1435
HpyCH4III	ACNGT	5 cut(s)	254, 561, 864, 1090, 1472
HpyCH4IV	ACGT	3 cut(s)	806, 936, 1333
HpyF10VI	GCNNNNNNNGC	2 cut(s)	512, 1124
HpyF3I	CTNAG	6 cut(s)	602, 1053, 1151, 1427, 1587, 1611
HpySE526I	ACGT	3 cut(s)	806, 936, 1333
Hsp92II	CATG	7 cut(s)	25, 498, 814, 882, 1062, 1426, 1607
HspAI	GCGC	2 cut(s)	1337, 1579
Kzo9I	GATC	3 cut(s)	115, 979, 1211
LguI	GCTCTTC	1 cut(s)	735
LmnI	GCTCC	1 cut(s)	1600
Lsp1109I	GCAGC	5 cut(s)	403, 700, 844, 1111, 1632
LweI	GCATC	3 cut(s)	481, 502, 654
MaeI	CTAG	5 cut(s)	210, 404, 800, 995, 1308
MaeII	ACGT	3 cut(s)	806, 936, 1333
MaeIII	GTNAC	7 cut(s)	158, 467, 802, 1329, 1354, 1431, 1520
MalI	GATC	3 cut(s)	117, 981, 1213
MboI	GATC	3 cut(s)	115, 979, 1211
MboII	GAAGA	4 cut(s)	752, 940, 1574, 1577
MflI	RGATCY	1 cut(s)	1211
MlyI	GAGTC	1 cut(s)	1504
MmeI	TCCRAC	1 cut(s)	442
MnlI	CCTC	8 cut(s)	559, 663, 775, 878, 1048, 1097, 1453, 1535
Mph1103I	ATGCAT	1 cut(s)	496
MroXI	GAANNNNTTC	1 cut(s)	1445
MseI	TTAA	3 cut(s)	486, 1377, 1596
MslI	CAYNNNNRTG	2 cut(s)	877, 907
MspI	CCGG	3 cut(s)	119, 413, 528
MspR9I	CCNGG	3 cut(s)	120, 399, 972
Mva1269I	GAATGC	1 cut(s)	1270
MvaI	CCWGG	2 cut(s)	399, 972
MwoI	GCNNNNNNNGC	2 cut(s)	512, 1124
NciI	CCSGG	1 cut(s)	120
NdeI	CATATG	2 cut(s)	754, 1533
NdeII	GATC	3 cut(s)	115, 979, 1211
NheI	GCTAGC	1 cut(s)	994
NlaIII	CATG	7 cut(s)	25, 498, 814, 882, 1062, 1426, 1607
NlaIV	GGNNCC	3 cut(s)	396, 786, 1464
NmeAIII	GCCGAG	3 cut(s)	693, 1294, 1562
NmuCI	GTSAC	4 cut(s)	467, 1329, 1354, 1431
NsbI	TGCGCA	1 cut(s)	1338
NsiI	ATGCAT	1 cut(s)	496
NspI	RCATGY	2 cut(s)	1062, 1426
NspV	TTCGAA	1 cut(s)	167
PaeI	GCATGC	1 cut(s)	1426
PciSI	GCTCTTC	1 cut(s)	735
PcsI	WCGNNNNNNNCGW	1 cut(s)	77
PctI	GAATGC	1 cut(s)	1270
PdmI	GAANNNNTTC	1 cut(s)	1445
PfeI	GAWTC	3 cut(s)	227, 587, 1393
PkrI	GCNGC	6 cut(s)	418, 517, 715, 859, 1126, 1622
PleI	GAGTC	1 cut(s)	1504
PmaCI	CACGTG	1 cut(s)	1334
PmlI	CACGTG	1 cut(s)	1334
PpsI	GAGTC	1 cut(s)	1504
Ppu21I	YACGTR	1 cut(s)	1334
PpuMI	RGGWCCY	1 cut(s)	784
PshBI	ATTAAT	1 cut(s)	1377
Psp5II	RGGWCCY	1 cut(s)	784
Psp6I	CCWGG	2 cut(s)	397, 970
PspCI	CACGTG	1 cut(s)	1334
PspFI	CCCAGC	1 cut(s)	1615
PspGI	CCWGG	2 cut(s)	397, 970
PspN4I	GGNNCC	3 cut(s)	396, 786, 1464
PspPI	GGNCC	2 cut(s)	784, 1462
PspPPI	RGGWCCY	1 cut(s)	784
PstNI	CAGNNNCTG	1 cut(s)	583
PsuI	RGATCY	1 cut(s)	1211
RsaI	GTAC	5 cut(s)	256, 295, 631, 643, 1074
RsaNI	GTAC	5 cut(s)	255, 294, 630, 642, 1073
RseI	CAYNNNNRTG	2 cut(s)	877, 907
SapI	GCTCTTC	1 cut(s)	735
SaqAI	TTAA	3 cut(s)	486, 1377, 1596
SatI	GCNGC	6 cut(s)	417, 516, 714, 858, 1125, 1621
Sau3AI	GATC	3 cut(s)	115, 979, 1211
Sau96I	GGNCC	2 cut(s)	784, 1462
SchI	GAGTC	1 cut(s)	1504
ScrFI	CCNGG	3 cut(s)	120, 399, 972
SfaNI	GCATC	3 cut(s)	481, 502, 654
SfcI	CTRYAG	1 cut(s)	852
SfuI	TTCGAA	1 cut(s)	167
SinI	GGWCC	1 cut(s)	784
SmiMI	CAYNNNNRTG	2 cut(s)	877, 907
SphI	GCATGC	1 cut(s)	1426
SsiI	CCGC	1 cut(s)	515
SspMI	CTAG	5 cut(s)	210, 404, 800, 995, 1308
StyD4I	CCNGG	3 cut(s)	118, 397, 970
StyI	CCWWGG	1 cut(s)	1138
TaaI	ACNGT	5 cut(s)	254, 561, 864, 1090, 1472
TaiI	ACGT	3 cut(s)	809, 939, 1336
TaqI	TCGA	3 cut(s)	167, 290, 1439
TatI	WGTACW	3 cut(s)	293, 629, 1072
TauI	GCSGC	1 cut(s)	518
TfiI	GAWTC	3 cut(s)	227, 587, 1393
Tru1I	TTAA	3 cut(s)	486, 1377, 1596
Tru9I	TTAA	3 cut(s)	486, 1377, 1596
TscAI	CASTG	4 cut(s)	118, 324, 559, 795
TseFI	GTSAC	4 cut(s)	467, 1329, 1354, 1431
TseI	GCWGC	5 cut(s)	416, 713, 857, 1124, 1620
Tsp45I	GTSAC	4 cut(s)	467, 1329, 1354, 1431
TspDTI	ATGAA	6 cut(s)	267, 342, 799, 891, 1002, 1485
TspGWI	ACGGA	2 cut(s)	711, 1006
TspRI	CASTG	4 cut(s)	118, 324, 559, 795
VpaK11BI	GGWCC	1 cut(s)	784
VspI	ATTAAT	1 cut(s)	1377
XapI	RAATTY	1 cut(s)	1362
XbaI	TCTAGA	1 cut(s)	209
XceI	RCATGY	2 cut(s)	1062, 1426
XmiI	GTMKAC	1 cut(s)	83
XmnI	GAANNNNTTC	1 cut(s)	1445
XspI	CTAG	5 cut(s)	210, 404, 800, 995, 1308
Zsp2I	ATGCAT	1 cut(s)	496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.