Rroxscaffold_4G00299100

Mechanosensitive ion channel protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005685
Physical Location & Seq
Reverse (-)
19037039 .. 19041108
4070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299100.1

Sequence Viewer

Length: 1647 bp
ATGGCTGGACGTAGGCTTTCCATGTTGAAGTCACTTTGCAAGTCTACCAATTGCTCTCTCAACCCAATGTCGTTTCAGTTGTCTAGTTCTTATGGGAAATTTGTGAGCTATGCTGATTCAGTTCTTGTAAGACCATCGTATGCTTTTGTGAATCGCGGTTACCATACGAAGGAAACAGAATCCCATAGAACTTATGTGAATACTCAGATTAAAGCACTGGGTTCTGGGTCACTTGTTAATACACAAGGTTACGGAATCAAACCCATTACAGCATTTTCACCCACATTGTCAAGTTCAGTTAGTAGGTGTGCTTTTCCTCCTGTTTCGATAAGCCCAATTCTGGGTTGCCGTTCATACTCATCATTTTTCGGTAGCAATGCTGATAAAGATGGGCAACCTGGAGTTCCGGCTTCTTCCAGCGGCAGTGAAGTGGATGTTGTTAATAGTAGCGTTGGTGGAAGTGATTTGATTGATAAGGTTAAGGAATTCGTGCAGAGTGCTGCGGATGCGGTAACTCATACTGGGCAGAAGGCTAAAGAAGCATCTAGTGAAATGTCTCCTTATGTTCAGCAATTGTTGGATACATATCCACATCTTAGAAATGTGGTTACTCCAGTTGGTTGTACTTTGGCAGGTACTATATTTGCTTGGCTAGTGATGCCTAGGCTTTTCAGGAGGTTTCACAAGTACGCAAGTCTAGGCCGCACTGCTTTACTATCTGGAAGCCTTGAGGAGCAAGTTCCATATGAGAAAAGTTTTTGGGGTGCTTTGGAGGATCCAGTGCGATATCTATTTACTTTTATGGCATTTTCACAGATTGGCACAATGGTTGCACCAACCGCTATAGCTGCTCAATATCTTGCACCAGCTTGGAGAGGTGCAGCTATCATTTCATTTGTTTGGTTTCTATATCGGTGGAAGACAAATGTGTATACTCGTGCATTAGCAACTAAAAGTTTGCCCAGCAGTCTTGATCGGGAGAAGTTGTTAGCTCTAGACAAAATTTCATCTGTTGGTCTTTTTATGATTGGGATAATGGCTCTAGCTGAGGCATGTGGGGTGGCTGTGCAATCTATTATGACTGTTGGTGGCATAGGAGGAGTTGCTACTGCTTTTGCTGCCAGAGATGTCCTTGGCAACGTGCTGAGTGGTTTATCTATGCAATTTACAAAGCCCTTTTCACTAGGTGATACAATAAAGGCTGGATCCATAGAAGGTCAAGTGGTGGATATGGGACTTACAACCACTTCATTACTGAATGCTGAGAAGTTCCCAGTGATAGTTCCCAATTCACTCTTTTCTAGTCAGGTGATTGTGAACAAGTCACGTGCTCAGTGGCGTGGCATCGTCACCAGAATTCCGCTGCAAATTAATGATGATTTGGATAAGGTTCCCCAAATATCAGATGATATAAAAAGCATGCTGAGATCACACCCGAAGGTTTTCTTGGGAAAGGAGGTGCCTTATTGTTTCCTTTCTCGTCTAGAGAGCTCATTTGGAGAATTGACTCTTGGATGTAACCTCAAACATATGAGCAAGGATGAGTTATTCTCTACAGAAGAAGATATCCTTTTGCGGTCGGTCAAGATAATTAAGGGGCATGGCGCCAAGTTGGGCAGCACCTATGGTGATATGACCACTACATAG

Protein Analysis

548

Amino Acids

59.38

Weight (kDa)

9.18

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 376 - 443 3.8e-16 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 623
AccB1I GGYRCC 2 cut(s) 1459, 1604
AccI GTMKAC 2 cut(s) 44, 932
AccII CGCG 1 cut(s) 156
AciI CCGC 8 cut(s) 156, 420, 503, 509, 703, 840, 1361, 1576
AclWI GGATC 4 cut(s) 770, 783, 1200, 1213
AcsI RAATTY 4 cut(s) 98, 485, 1002, 1356
AcvI CACGTG 1 cut(s) 1328
AcyI GRCGYC 1 cut(s) 1605
AdeI CACNNNGTG 1 cut(s) 1187
AfaI GTAC 3 cut(s) 625, 637, 689
AfiI CCNNNNNNNGG 3 cut(s) 169, 340, 341
AgsI TTSAA 1 cut(s) 28
AjnI CCWGG 1 cut(s) 397
AjuI GAANNNNNNNTTGG 4 cut(s) 1430, 1462, 1494, 1526
AluBI AGCT 7 cut(s) 108, 848, 869, 884, 992, 1046, 1491
AluI AGCT 7 cut(s) 108, 848, 869, 884, 992, 1046, 1491
Alw21I GWGCWC 2 cut(s) 1333, 1493
Alw26I GTCTC 1 cut(s) 561
AlwI GGATC 4 cut(s) 770, 783, 1200, 1213
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 6 cut(s) 500, 848, 881, 1118, 1363, 1617
ApoI RAATTY 4 cut(s) 98, 485, 1002, 1356
AseI ATTAAT 1 cut(s) 1371
Asp700I GAANNNNTTC 1 cut(s) 1442
AspA2I CCTAGG 1 cut(s) 662
AspLEI GCGC 1 cut(s) 1607
AsuHPI GGTGA 5 cut(s) 270, 1199, 1321, 1342, 1640
AvrII CCTAGG 1 cut(s) 662
BamHI GGATCC 2 cut(s) 775, 1205
BanI GGYRCC 2 cut(s) 1459, 1604
BanII GRGCYC 1 cut(s) 1493
BauI CACGAG 1 cut(s) 936
BbrPI CACGTG 1 cut(s) 1328
BbsI GAAGAC 1 cut(s) 926
Bbv12I GWGCWC 2 cut(s) 1333, 1493
BbvCI CCTCAGC 1 cut(s) 1047
BbvI GCAGC 6 cut(s) 487, 835, 893, 1105, 1350, 1629
BccI CCATC 2 cut(s) 142, 383
BceAI ACGGC 1 cut(s) 333
BciT130I CCWGG 1 cut(s) 399
BciVI GTATCC 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 843, 1554
BfoI RGCGCY 1 cut(s) 1608
BfuAI ACCTGC 1 cut(s) 623
BfuI GTATCC 1 cut(s) 574
BisI GCNGC 8 cut(s) 421, 501, 703, 849, 882, 1119, 1364, 1618
BlnI CCTAGG 1 cut(s) 662
BlsI GCNGC 8 cut(s) 422, 502, 704, 850, 883, 1120, 1365, 1619
Bme1390I CCNGG 1 cut(s) 399
BmiI GGNNCC 5 cut(s) 777, 1207, 1392, 1461, 1606
BmrFI CCNGG 1 cut(s) 399
BmrI ACTGGG 3 cut(s) 227, 531, 1268
BmsI GCATC 4 cut(s) 496, 551, 648, 1353
BmuI ACTGGG 3 cut(s) 227, 531, 1268
BpiI GAAGAC 1 cut(s) 926
BplI GAGNNNNNCTC 2 cut(s) 1535, 1567
BpmI CTGGAG 2 cut(s) 420, 597
Bpu10I CCTNAGC 1 cut(s) 1047
BpuEI CTTGAG 1 cut(s) 749
BsaAI YACGTR 1 cut(s) 1328
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 1605
BsaJI CCNNGG 2 cut(s) 662, 1132
BsaXI ACNNNNNCTCC 2 cut(s) 971, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 340, 341
Bse1I ACTGG 5 cut(s) 222, 526, 614, 779, 1274
Bse3DI GCAATG 1 cut(s) 382
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 2 cut(s) 662, 1132
BseGI GGATG 4 cut(s) 439, 511, 1520, 1546
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 169, 340, 341
BseMI GCAATG 1 cut(s) 382
BseMII CTCAG 6 cut(s) 218, 1038, 1136, 1254, 1346, 1415
BseNI ACTGG 5 cut(s) 222, 526, 614, 779, 1274
BseRI GAGGAG 2 cut(s) 746, 1113
BseXI GCAGC 6 cut(s) 487, 835, 893, 1105, 1350, 1629
BseYI CCCAGC 1 cut(s) 962
BsgI GTGCAG 2 cut(s) 512, 900
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 1580
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 2 cut(s) 1459, 1604
BsiEI CGRYCG 1 cut(s) 1580
BsiHKAI GWGCWC 2 cut(s) 1333, 1493
BsiSI CCGG 1 cut(s) 407
BslFI GGGAC 1 cut(s) 1248
BslI CCNNNNNNNGG 3 cut(s) 169, 340, 341
BsmAI GTCTC 1 cut(s) 561
BsmFI GGGAC 1 cut(s) 1248
BsmI GAATGC 1 cut(s) 1264
BsnI GGCC 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 1333, 1493
Bsp143I GATC 4 cut(s) 775, 973, 1205, 1427
BspACI CCGC 8 cut(s) 156, 420, 503, 509, 703, 840, 1361, 1576
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 6 cut(s) 217, 1039, 1137, 1255, 1345, 1416
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 5 cut(s) 777, 1207, 1392, 1461, 1606
BspMI ACCTGC 1 cut(s) 623
BspPI GGATC 4 cut(s) 770, 783, 1200, 1213
BspT107I GGYRCC 2 cut(s) 1459, 1604
BsrDI GCAATG 1 cut(s) 382
BsrI ACTGG 5 cut(s) 222, 526, 614, 779, 1274
BssECI CCNNGG 2 cut(s) 662, 1132
BssMI GATC 4 cut(s) 775, 973, 1205, 1427
BssNAI GTATAC 1 cut(s) 933
BssNI GRCGYC 1 cut(s) 1605
BssSI CACGAG 1 cut(s) 936
BssT1I CCWWGG 2 cut(s) 662, 1132
Bst1107I GTATAC 1 cut(s) 933
Bst2BI CACGAG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 1084
BstACI GRCGYC 1 cut(s) 1605
BstBAI YACGTR 1 cut(s) 1328
BstC8I GCNNGC 1 cut(s) 1421
BstDEI CTNAG 7 cut(s) 204, 596, 1047, 1145, 1263, 1332, 1424
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 4 cut(s) 439, 511, 1520, 1546
BstFNI CGCG 1 cut(s) 156
BstH2I RGCGCY 1 cut(s) 1608
BstHHI GCGC 1 cut(s) 1607
BstKTI GATC 4 cut(s) 778, 976, 1208, 1430
BstMAI GTCTC 1 cut(s) 561
BstMBI GATC 4 cut(s) 775, 973, 1205, 1427
BstMCI CGRYCG 1 cut(s) 1580
BstMWI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
BstNI CCWGG 1 cut(s) 399
BstNSI RCATGY 2 cut(s) 1056, 1423
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 397
BstSFI CTRYAG 2 cut(s) 843, 1554
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 6 cut(s) 487, 835, 893, 1105, 1350, 1629
BstV2I GAAGAC 1 cut(s) 926
BstX2I RGATCY 2 cut(s) 775, 1205
BstYI RGATCY 2 cut(s) 775, 1205
BstZ17I GTATAC 1 cut(s) 933
BsuI GTATCC 1 cut(s) 574
BsuRI GGCC 1 cut(s) 702
BtsCI GGATG 4 cut(s) 439, 511, 1520, 1546
BtsI GCAGTG 2 cut(s) 430, 705
BtsIMutI CAGTG 6 cut(s) 215, 430, 705, 786, 1281, 1340
BveI ACCTGC 1 cut(s) 623
Cac8I GCNNGC 1 cut(s) 1421
CfoI GCGC 1 cut(s) 1607
Csp6I GTAC 3 cut(s) 624, 636, 688
CviAII CATG 4 cut(s) 22, 1053, 1420, 1601
CviQI GTAC 3 cut(s) 624, 636, 688
DdeI CTNAG 7 cut(s) 204, 596, 1047, 1145, 1263, 1332, 1424
DinI GGCGCC 1 cut(s) 1606
DpnI GATC 4 cut(s) 777, 975, 1207, 1429
DpnII GATC 4 cut(s) 775, 973, 1205, 1427
DraIII CACNNNGTG 1 cut(s) 1187
Ecl136II GAGCTC 1 cut(s) 1491
Eco130I CCWWGG 2 cut(s) 662, 1132
Eco24I GRGCYC 1 cut(s) 1493
Eco32I GATATC 2 cut(s) 788, 1567
Eco53kI GAGCTC 1 cut(s) 1491
Eco72I CACGTG 1 cut(s) 1328
Eco91I GGTNACC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 1491
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 2 cut(s) 485, 1356
EcoRII CCWGG 1 cut(s) 397
EcoRV GATATC 2 cut(s) 788, 1567
EcoT14I CCWWGG 2 cut(s) 662, 1132
EcoT38I GRGCYC 1 cut(s) 1493
EgeI GGCGCC 1 cut(s) 1606
EheI GGCGCC 1 cut(s) 1606
ErhI CCWWGG 2 cut(s) 662, 1132
FaeI CATG 4 cut(s) 25, 1056, 1423, 1604
FalI AAGNNNNNCTT 4 cut(s) 1430, 1462, 1554, 1586
FaqI GGGAC 1 cut(s) 1248
FatI CATG 4 cut(s) 21, 1052, 1419, 1600
FauNDI CATATG 2 cut(s) 745, 1530
FblI GTMKAC 2 cut(s) 44, 932
Fnu4HI GCNGC 8 cut(s) 421, 501, 703, 849, 882, 1119, 1364, 1618
FokI GGATG 4 cut(s) 446, 518, 1527, 1553
FriOI GRGCYC 1 cut(s) 1493
Fsp4HI GCNGC 8 cut(s) 421, 501, 703, 849, 882, 1119, 1364, 1618
GlaI GCGC 1 cut(s) 1606
GluI GCNGC 8 cut(s) 421, 501, 703, 849, 882, 1119, 1364, 1618
GsaI CCCAGC 1 cut(s) 966
GsuI CTGGAG 2 cut(s) 420, 597
HaeII RGCGCY 1 cut(s) 1608
HaeIII GGCC 1 cut(s) 702
HapII CCGG 1 cut(s) 407
HhaI GCGC 1 cut(s) 1607
Hin1I GRCGYC 1 cut(s) 1605
Hin1II CATG 4 cut(s) 25, 1056, 1423, 1604
Hin6I GCGC 1 cut(s) 1605
HinP1I GCGC 1 cut(s) 1605
HinfI GANTC 5 cut(s) 116, 151, 179, 255, 1507
HpaII CCGG 1 cut(s) 407
HphI GGTGA 5 cut(s) 270, 1199, 1321, 1342, 1640
Hpy166II GTNNAC 3 cut(s) 45, 933, 1318
Hpy188I TCNGA 2 cut(s) 207, 1405
Hpy188III TCNNGA 7 cut(s) 673, 720, 971, 977, 995, 1484, 1585
Hpy8I GTNNAC 3 cut(s) 45, 933, 1318
HpyAV CCTTC 4 cut(s) 163, 523, 1208, 1432
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 3 cut(s) 10, 1140, 1327
HpyCH4V TGCA 9 cut(s) 39, 493, 833, 863, 881, 941, 1069, 1162, 1366
HpyF10VI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
HpyF3I CTNAG 7 cut(s) 204, 596, 1047, 1145, 1263, 1332, 1424
HpySE526I ACGT 3 cut(s) 10, 1140, 1327
Hsp92I GRCGYC 1 cut(s) 1605
Hsp92II CATG 4 cut(s) 25, 1056, 1423, 1604
HspAI GCGC 1 cut(s) 1605
KasI GGCGCC 1 cut(s) 1604
Kzo9I GATC 4 cut(s) 775, 973, 1205, 1427
LmnI GCTCC 1 cut(s) 733
Lsp1109I GCAGC 6 cut(s) 487, 835, 893, 1105, 1350, 1629
LweI GCATC 4 cut(s) 496, 551, 648, 1353
MaeII ACGT 3 cut(s) 10, 1140, 1327
MaeIII GTNAC 9 cut(s) 30, 158, 228, 248, 511, 607, 1323, 1348, 1517
MalI GATC 4 cut(s) 777, 975, 1207, 1429
MboI GATC 4 cut(s) 775, 973, 1205, 1427
MboII GAAGA 4 cut(s) 405, 931, 1571, 1574
MfeI CAATTG 2 cut(s) 49, 572
MflI RGATCY 2 cut(s) 775, 1205
MhlI GDGCHC 2 cut(s) 1333, 1493
Mly113I GGCGCC 1 cut(s) 1605
MlyI GAGTC 1 cut(s) 1501
MmeI TCCRAC 1 cut(s) 558
MnlI CCTC 9 cut(s) 327, 669, 724, 766, 869, 1042, 1091, 1450, 1532
MroXI GAANNNNTTC 1 cut(s) 1442
MseI TTAA 6 cut(s) 210, 237, 441, 480, 1371, 1593
MspA1I CMGCKG 2 cut(s) 420, 1363
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 1 cut(s) 399
MunI CAATTG 2 cut(s) 49, 572
Mva1269I GAATGC 1 cut(s) 1264
MvaI CCWGG 1 cut(s) 399
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 6 cut(s) 506, 539, 658, 839, 848, 1118
NarI GGCGCC 1 cut(s) 1605
NdeI CATATG 2 cut(s) 745, 1530
NdeII GATC 4 cut(s) 775, 973, 1205, 1427
NlaIII CATG 4 cut(s) 25, 1056, 1423, 1604
NlaIV GGNNCC 5 cut(s) 777, 1207, 1392, 1461, 1606
NmuCI GTSAC 4 cut(s) 30, 228, 1323, 1348
NspI RCATGY 2 cut(s) 1056, 1423
PaeI GCATGC 1 cut(s) 1423
PctI GAATGC 1 cut(s) 1264
PdmI GAANNNNTTC 1 cut(s) 1442
PfeI GAWTC 4 cut(s) 116, 151, 179, 255
PkrI GCNGC 8 cut(s) 422, 502, 704, 850, 883, 1120, 1365, 1619
PleI GAGTC 1 cut(s) 1501
PluTI GGCGCC 1 cut(s) 1608
PmaCI CACGTG 1 cut(s) 1328
PmlI CACGTG 1 cut(s) 1328
PpsI GAGTC 1 cut(s) 1501
Ppu21I YACGTR 1 cut(s) 1328
PshBI ATTAAT 1 cut(s) 1371
Psp124BI GAGCTC 1 cut(s) 1493
Psp6I CCWGG 1 cut(s) 397
PspCI CACGTG 1 cut(s) 1328
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 962
PspGI CCWGG 1 cut(s) 397
PspN4I GGNNCC 5 cut(s) 777, 1207, 1392, 1461, 1606
PsuI RGATCY 2 cut(s) 775, 1205
RsaI GTAC 3 cut(s) 625, 637, 689
RsaNI GTAC 3 cut(s) 624, 636, 688
SacI GAGCTC 1 cut(s) 1493
SaqAI TTAA 6 cut(s) 210, 237, 441, 480, 1371, 1593
SatI GCNGC 8 cut(s) 421, 501, 703, 849, 882, 1119, 1364, 1618
Sau3AI GATC 4 cut(s) 775, 973, 1205, 1427
SchI GAGTC 1 cut(s) 1501
ScrFI CCNGG 1 cut(s) 399
SduI GDGCHC 2 cut(s) 1333, 1493
SfaNI GCATC 4 cut(s) 496, 551, 648, 1353
SfcI CTRYAG 2 cut(s) 843, 1554
SfoI GGCGCC 1 cut(s) 1606
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SphI GCATGC 1 cut(s) 1423
SsiI CCGC 8 cut(s) 156, 420, 503, 509, 703, 840, 1361, 1576
SspDI GGCGCC 1 cut(s) 1604
SstI GAGCTC 1 cut(s) 1493
StyD4I CCNGG 1 cut(s) 397
StyI CCWWGG 2 cut(s) 662, 1132
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 3 cut(s) 13, 1143, 1330
TaqI TCGA 1 cut(s) 326
TaqII GACCGA 1 cut(s) 1570
TatI WGTACW 1 cut(s) 623
TauI GCSGC 2 cut(s) 423, 705
TfiI GAWTC 4 cut(s) 116, 151, 179, 255
Tru1I TTAA 6 cut(s) 210, 237, 441, 480, 1371, 1593
Tru9I TTAA 6 cut(s) 210, 237, 441, 480, 1371, 1593
TscAI CASTG 6 cut(s) 222, 430, 712, 786, 1281, 1340
TseFI GTSAC 4 cut(s) 30, 228, 1323, 1348
TseI GCWGC 6 cut(s) 500, 848, 881, 1118, 1363, 1617
Tsp45I GTSAC 4 cut(s) 30, 228, 1323, 1348
TspDTI ATGAA 4 cut(s) 342, 882, 996, 1239
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 6 cut(s) 222, 430, 712, 786, 1281, 1340
VspI ATTAAT 1 cut(s) 1371
XapI RAATTY 4 cut(s) 98, 485, 1002, 1356
XbaI TCTAGA 2 cut(s) 994, 1483
XceI RCATGY 2 cut(s) 1056, 1423
XmaJI CCTAGG 1 cut(s) 662
XmiI GTMKAC 2 cut(s) 44, 932
XmnI GAANNNNTTC 1 cut(s) 1442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.