AT4G01200

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009207
Physical Location & Seq
Reverse (-)
506511 .. 507626
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G01200.1

Sequence Viewer

Length: 753 bp
ATGGAGGAGACGCAAGTTCTTGAGATCAATCTCATCTCTGCGCAAGGACTTAAAGAACCGACGGGAAAGCTCCGGCGATTGCAGACTTACGCATCCGTCTGGGTTGATTCTTCCAGCAAACTCCGGACTCGAATTGATCGGATCGGCTCTGAGAATCCGATCTGGAACGACAAATTCGTTTTCCAGGTATCGCCGGAGTTTCTATCTAGCGAAACTTCTGGTGTTTCAATCGAGATCTACGCCGTTGGTTACTTGCGAGATCATCTGATCGGAACTGTTCGCTTCCTCGTGAGTAACTTCCTCCCAACCGCCGCCGTGAAAGTTCCATCTCTCGTCGCGCTTCAGATCCGTCGTCCTTCTGGAAAGTTTCACGGCGTTCTTAACATCGCGGCTATGGTGATGGATGCTTCTGAACTTCCAGCTGATTTCTTCAAGTCGGTTCAAGAGATCCGTCGGAGTCGGAAAATGAGAAAGTCTAGATCTGCGGTTAGTTCGTCGGAGAACGGTTCCGCTGACGACGGTGAGAGTTCGAAAGAGAATTCGGTTTGCGGATCGGTTAATTTCTCCGACGACGGTACTGACTCGACGGCGTCTTCTCCGATGCCGTCTCCTCTCAGAGACTGGAACGCTGTTCGAAATTTGGCGGGAAAGAATCACGTGCGATCGTCATCGGACGGCGGAGGATTGATGTGCTGTTTCTTGATGAAATCGTCGGGCATGTTACCACCAAGAAGAAAAATGACGGACGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

250

Amino Acids

27.37

Weight (kDa)

9.19

Isoelectric Point (pI)

63.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 6 - 100 2.1e-13 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 42
AccII CGCG 2 cut(s) 338, 389
AccIII TCCGGA 1 cut(s) 123
AciI CCGC 8 cut(s) 309, 312, 389, 485, 510, 549, 644, 678
AclWI GGATC 4 cut(s) 149, 340, 442, 559
AcsI RAATTY 3 cut(s) 173, 538, 637
AcuI CTGAAG 1 cut(s) 326
AcvI CACGTG 1 cut(s) 658
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 1 cut(s) 577
AgsI TTSAA 3 cut(s) 228, 433, 443
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 2 cut(s) 70, 422
AluI AGCT 2 cut(s) 70, 422
Alw26I GTCTC 3 cut(s) 2, 612, 612
AlwI GGATC 4 cut(s) 149, 340, 442, 559
AlwNI CAGNNNCTG 1 cut(s) 621
Aor13HI TCCGGA 1 cut(s) 123
ApoI RAATTY 3 cut(s) 173, 538, 637
AspLEI GCGC 2 cut(s) 43, 340
AsuHPI GGTGA 2 cut(s) 409, 533
AsuII TTCGAA 2 cut(s) 530, 634
BauI CACGAG 1 cut(s) 287
BbrPI CACGTG 1 cut(s) 658
BbsI GAAGAC 1 cut(s) 585
BccI CCATC 2 cut(s) 334, 394
BceAI ACGGC 6 cut(s) 227, 299, 388, 589, 603, 691
BciT130I CCWGG 1 cut(s) 185
BcoDI GTCTC 3 cut(s) 2, 612, 612
BfaI CTAG 2 cut(s) 207, 477
BglII AGATCT 2 cut(s) 234, 479
BisI GCNGC 2 cut(s) 312, 390
BlsI GCNGC 2 cut(s) 313, 391
Bme1390I CCNGG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 3 cut(s) 101, 394, 591
BpiI GAAGAC 1 cut(s) 585
Bpu14I TTCGAA 2 cut(s) 530, 634
BpuEI CTTGAG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 658
BsaBI GATNNNNATC 1 cut(s) 667
BsaHI GRCGYC 1 cut(s) 590
BsaWI WCCGGW 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 626
Bse8I GATNNNNATC 1 cut(s) 667
BseAI TCCGGA 1 cut(s) 123
BseBI CCWGG 1 cut(s) 185
BseGI GGATG 2 cut(s) 92, 409
BseJI GATNNNNATC 1 cut(s) 667
BseMII CTCAG 2 cut(s) 141, 628
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 2 cut(s) 20, 600
Bsh1236I CGCG 2 cut(s) 338, 389
Bsh1285I CGRYCG 1 cut(s) 665
BsiEI CGRYCG 1 cut(s) 665
BsiSI CCGG 3 cut(s) 73, 124, 194
BsmAI GTCTC 3 cut(s) 2, 612, 612
BsmBI CGTCTC 2 cut(s) 2, 612
Bsp119I TTCGAA 2 cut(s) 530, 634
Bsp13I TCCGGA 1 cut(s) 123
BspACI CCGC 8 cut(s) 309, 312, 389, 485, 510, 549, 644, 678
BspCNI CTCAG 2 cut(s) 142, 627
BspEI TCCGGA 1 cut(s) 123
BspFNI CGCG 2 cut(s) 338, 389
BspLI GGNNCC 1 cut(s) 508
BspPI GGATC 4 cut(s) 149, 340, 442, 559
BspT104I TTCGAA 2 cut(s) 530, 634
BsrI ACTGG 1 cut(s) 626
BssNI GRCGYC 1 cut(s) 590
BssSI CACGAG 1 cut(s) 287
Bst2BI CACGAG 1 cut(s) 287
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 4 cut(s) 277, 506, 521, 575
BstACI GRCGYC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 658
BstBI TTCGAA 2 cut(s) 530, 634
BstDEI CTNAG 2 cut(s) 150, 614
BstF5I GGATG 2 cut(s) 92, 409
BstFNI CGCG 2 cut(s) 338, 389
BstHHI GCGC 2 cut(s) 43, 340
BstMAI GTCTC 3 cut(s) 2, 612, 612
BstMCI CGRYCG 1 cut(s) 665
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 1 cut(s) 183
BstUI CGCG 2 cut(s) 338, 389
BstV2I GAAGAC 1 cut(s) 585
BstX2I RGATCY 4 cut(s) 234, 345, 447, 479
BstYI RGATCY 4 cut(s) 234, 345, 447, 479
BtgZI GCGATG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 92, 409
CaiI CAGNNNCTG 1 cut(s) 621
CfoI GCGC 2 cut(s) 43, 340
CseI GACGC 2 cut(s) 19, 579
Csp6I GTAC 1 cut(s) 576
CviAII CATG 1 cut(s) 718
CviJI RGCY 5 cut(s) 70, 147, 392, 422, 750
CviKI_1 RGCY 5 cut(s) 70, 147, 392, 422, 750
CviQI GTAC 1 cut(s) 576
DdeI CTNAG 2 cut(s) 150, 614
EciI GGCGGA 1 cut(s) 693
Eco57I CTGAAG 1 cut(s) 326
Eco72I CACGTG 1 cut(s) 658
EcoRI GAATTC 1 cut(s) 538
EcoRII CCWGG 1 cut(s) 183
Esp3I CGTCTC 2 cut(s) 2, 612
FaeI CATG 1 cut(s) 721
FaiI YATR 2 cut(s) 395, 719
FatI CATG 1 cut(s) 717
FauI CCCGC 1 cut(s) 637
Fnu4HI GCNGC 2 cut(s) 312, 390
FokI GGATG 2 cut(s) 79, 416
Fsp4HI GCNGC 2 cut(s) 312, 390
FspBI CTAG 2 cut(s) 207, 477
FspI TGCGCA 1 cut(s) 42
GlaI GCGC 2 cut(s) 42, 339
GluI GCNGC 2 cut(s) 312, 390
HapII CCGG 3 cut(s) 73, 124, 194
HgaI GACGC 2 cut(s) 19, 579
HhaI GCGC 2 cut(s) 43, 340
Hin1I GRCGYC 1 cut(s) 590
Hin1II CATG 1 cut(s) 721
Hin6I GCGC 2 cut(s) 41, 338
HinP1I GCGC 2 cut(s) 41, 338
HinfI GANTC 6 cut(s) 107, 127, 154, 457, 581, 652
HpaII CCGG 3 cut(s) 73, 124, 194
HphI GGTGA 2 cut(s) 409, 533
Hpy188III TCNNGA 9 cut(s) 20, 124, 163, 232, 289, 360, 443, 477, 700
HpyAV CCTTC 1 cut(s) 366
HpyCH4III ACNGT 4 cut(s) 277, 506, 521, 575
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 1 cut(s) 82
HpyF3I CTNAG 2 cut(s) 150, 614
HpySE526I ACGT 1 cut(s) 657
Hsp92I GRCGYC 1 cut(s) 590
Hsp92II CATG 1 cut(s) 721
HspAI GCGC 2 cut(s) 41, 338
Kpn2I TCCGGA 1 cut(s) 123
LmnI GCTCC 1 cut(s) 75
LweI GCATC 3 cut(s) 101, 394, 591
MaeI CTAG 2 cut(s) 207, 477
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 3 cut(s) 248, 293, 720
MboII GAAGA 4 cut(s) 102, 421, 585, 744
MflI RGATCY 4 cut(s) 234, 345, 447, 479
MluCI AATT 5 cut(s) 132, 173, 538, 559, 637
MlyI GAGTC 3 cut(s) 121, 466, 575
MmeI TCCRAC 4 cut(s) 434, 440, 477, 591
MnlI CCTC 4 cut(s) 296, 311, 621, 674
MroI TCCGGA 1 cut(s) 123
MseI TTAA 3 cut(s) 51, 381, 558
MspA1I CMGCKG 2 cut(s) 422, 512
MspI CCGG 3 cut(s) 73, 124, 194
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 2 cut(s) 338, 389
NlaIII CATG 1 cut(s) 721
NlaIV GGNNCC 1 cut(s) 508
NsbI TGCGCA 1 cut(s) 42
NspI RCATGY 1 cut(s) 721
NspV TTCGAA 2 cut(s) 530, 634
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 3 cut(s) 107, 154, 652
PflFI GACNNNGTC 1 cut(s) 589
PkrI GCNGC 2 cut(s) 313, 391
Ple19I CGATCG 1 cut(s) 665
PleI GAGTC 3 cut(s) 121, 465, 575
PmaCI CACGTG 1 cut(s) 658
PmlI CACGTG 1 cut(s) 658
PpsI GAGTC 3 cut(s) 121, 465, 575
Ppu21I YACGTR 1 cut(s) 658
Psp6I CCWGG 1 cut(s) 183
PspCI CACGTG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 508
PstNI CAGNNNCTG 1 cut(s) 621
PsuI RGATCY 4 cut(s) 234, 345, 447, 479
PsyI GACNNNGTC 1 cut(s) 589
PvuI CGATCG 1 cut(s) 665
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 1 cut(s) 577
RsaNI GTAC 1 cut(s) 576
SaqAI TTAA 3 cut(s) 51, 381, 558
SatI GCNGC 2 cut(s) 312, 390
SchI GAGTC 3 cut(s) 121, 466, 575
ScrFI CCNGG 1 cut(s) 185
SetI ASST 4 cut(s) 72, 189, 424, 660
SfaNI GCATC 3 cut(s) 101, 394, 591
SfuI TTCGAA 2 cut(s) 530, 634
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 5 cut(s) 132, 173, 538, 559, 637
SsiI CCGC 8 cut(s) 309, 312, 389, 485, 510, 549, 644, 678
SspMI CTAG 2 cut(s) 207, 477
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 4 cut(s) 277, 506, 521, 575
TaiI ACGT 1 cut(s) 660
TaqI TCGA 5 cut(s) 130, 231, 530, 584, 634
TasI AATT 5 cut(s) 132, 173, 538, 559, 637
TauI GCSGC 2 cut(s) 314, 392
TfiI GAWTC 3 cut(s) 107, 154, 652
Tru1I TTAA 3 cut(s) 51, 381, 558
Tru9I TTAA 3 cut(s) 51, 381, 558
TspDTI ATGAA 1 cut(s) 719
TspGWI ACGGA 3 cut(s) 85, 338, 440
Tth111I GACNNNGTC 1 cut(s) 589
XapI RAATTY 3 cut(s) 173, 538, 637
XbaI TCTAGA 1 cut(s) 476
XceI RCATGY 1 cut(s) 721
XspI CTAG 2 cut(s) 207, 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.