Prupe.2G181800_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0009207
Physical Location & Seq
Forward (+)
22450736 .. 22452410
1675 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G181800.1

Sequence Viewer

Length: 819 bp
ATGAAGAAAGAAAACGGAGATCACGAGGGCACGATGCGCGTGCTTGAGATCCACCTCATCTCCGCCCAGGGCCTCAAAGCCCCCACCGGTACCTTGCGCCGCATGCAGACCTACGCACTCGCCTGGGTCGACTCGGCCCACAAGCTCCGATCCCGGGTCGACAAGATCGGAGCCTCGAACCCAACCTGGAACGACCGGTTCCTCTTCAAGGTTCCGCCGGGCTTCCTCTCCAGCGAGACCTCCGCCGTCTCCGTCCAGATTTACACCGTCGGTACTTTCCGCGATCACCTCGTCGGCTCCGTCCGATTTCTGATCAACAATTTTCTTGACGTCCACTCCAAGATGCCGTCGTTTACCGCGGTCCAGATCCGGCGACCTTCTGGAAGGTTCTACGGCGTGTTGAATGTTGGAGCGATGGTGATCGACGGCTCTGATCTGGCTCCGGCGGTGAATGAGATGTCGGCGATCGGATACCGCGATCTGATGGGGAAGGGAGAGAGCTTCGGGCGCAAGCGCCGGCACGGCGACAGCATGAGGATCAAGTCGAAGGATTACTCTGGCGACTCAAAGGAGAATTCTTGTGCCGAGTCAACGGAGAACTCGGACGCTGGCGAGTCGGTGGCGTCATCTCCGGGGACGCCATTGCCGCCTCTGAAGGAGCTGAACGGGGTAGGAGATTTGGCGGGAACAACAACCAAGGTGTTGAGGGCTGCTCCATCGGACGGCTCTCGTTTCTTGTGCTGCTTGCTGACGCAGAGGAAGATTCAATACACTCCGACGGATCAGAACCCGGATGGGGCCCACAGCAGAGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

29.72

Weight (kDa)

9.36

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 333
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 2 cut(s) 129, 159
AccII CGCG 4 cut(s) 39, 282, 359, 477
AclWI GGATC 5 cut(s) 43, 144, 361, 545, 789
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 1 cut(s) 674
AcyI GRCGYC 3 cut(s) 330, 623, 638
AfaI GTAC 2 cut(s) 91, 274
AfiI CCNNNNNNNGG 4 cut(s) 154, 208, 722, 796
AgeI ACCGGT 2 cut(s) 86, 195
AgsI TTSAA 3 cut(s) 208, 403, 767
AjnI CCWGG 3 cut(s) 66, 122, 185
AluBI AGCT 3 cut(s) 145, 501, 661
AluI AGCT 3 cut(s) 145, 501, 661
Alw26I GTCTC 2 cut(s) 230, 253
AlwI GGATC 5 cut(s) 43, 144, 361, 545, 789
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 3 cut(s) 70, 135, 798
ApaI GGGCCC 1 cut(s) 802
ApeKI GCWGC 2 cut(s) 710, 741
ApoI RAATTY 1 cut(s) 574
AsiGI ACCGGT 2 cut(s) 86, 195
Asp718I GGTACC 1 cut(s) 89
AspLEI GCGC 4 cut(s) 39, 99, 510, 516
AspS9I GGNCC 5 cut(s) 70, 136, 361, 798, 799
AsuC2I CCSGG 5 cut(s) 154, 155, 219, 633, 791
AsuHPI GGTGA 3 cut(s) 278, 430, 460
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 361
BaeGI GKGCMC 2 cut(s) 32, 802
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 802
BarI GAAGNNNNNNTAC 2 cut(s) 752, 784
BauI CACGAG 1 cut(s) 23
BbvI GCAGC 2 cut(s) 697, 728
BccI CCATC 4 cut(s) 409, 478, 724, 788
BceAI ACGGC 6 cut(s) 230, 331, 409, 442, 538, 739
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 3 cut(s) 68, 124, 187
BciVI GTATCC 1 cut(s) 464
BclI TGATCA 1 cut(s) 312
BcnI CCSGG 5 cut(s) 154, 155, 219, 633, 791
BcoDI GTCTC 2 cut(s) 230, 253
BfoI RGCGCY 1 cut(s) 517
BfuI GTATCC 1 cut(s) 464
BglI GCCNNNNNGGC 1 cut(s) 522
BisI GCNGC 4 cut(s) 100, 647, 711, 742
BlsI GCNGC 4 cut(s) 101, 648, 712, 743
Bme1390I CCNGG 8 cut(s) 68, 124, 154, 155, 187, 219, 633, 791
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 5 cut(s) 70, 136, 361, 798, 799
BmiI GGNNCC 8 cut(s) 91, 172, 200, 213, 298, 441, 799, 800
BmrFI CCNGG 8 cut(s) 68, 124, 154, 155, 187, 219, 633, 791
BmsI GCATC 2 cut(s) 24, 333
BplI GAGNNNNNCTC 2 cut(s) 697, 729
BpmI CTGGAG 1 cut(s) 214
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 5 cut(s) 154, 155, 219, 633, 791
BsaBI GATNNNNATC 1 cut(s) 419
BsaHI GRCGYC 3 cut(s) 330, 623, 638
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 7 cut(s) 66, 67, 123, 153, 357, 632, 696
BsaWI WCCGGW 2 cut(s) 86, 195
BsaXI ACNNNNNCTCC 6 cut(s) 44, 74, 320, 350, 402, 432
Bsc4I CCNNNNNNNGG 4 cut(s) 154, 208, 722, 796
Bse118I RCCGGY 3 cut(s) 86, 195, 516
Bse3DI GCAATG 1 cut(s) 641
Bse8I GATNNNNATC 1 cut(s) 419
BseBI CCWGG 3 cut(s) 68, 124, 187
BseDI CCNNGG 7 cut(s) 66, 67, 123, 153, 357, 632, 696
BseGI GGATG 1 cut(s) 799
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 4 cut(s) 154, 208, 722, 796
BseMI GCAATG 1 cut(s) 641
BseSI GKGCMC 2 cut(s) 32, 802
BseXI GCAGC 2 cut(s) 697, 728
Bsh1236I CGCG 4 cut(s) 39, 282, 359, 477
Bsh1285I CGRYCG 2 cut(s) 196, 468
BshFI GGCC 3 cut(s) 72, 137, 800
BshNI GGYRCC 1 cut(s) 89
BshTI ACCGGT 2 cut(s) 86, 195
BsiEI CGRYCG 2 cut(s) 196, 468
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 9 cut(s) 87, 154, 196, 218, 370, 443, 517, 632, 791
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 4 cut(s) 154, 208, 722, 796
BsmAI GTCTC 2 cut(s) 230, 253
BsmBI CGTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 649
BsnI GGCC 3 cut(s) 72, 137, 800
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 153
Bsp120I GGGCCC 1 cut(s) 798
Bsp1286I GDGCHC 2 cut(s) 32, 802
BspANI GGCC 3 cut(s) 72, 137, 800
BspFNI CGCG 4 cut(s) 39, 282, 359, 477
BspLI GGNNCC 8 cut(s) 91, 172, 200, 213, 298, 441, 799, 800
BspPI GGATC 5 cut(s) 43, 144, 361, 545, 789
BspT107I GGYRCC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 641
BsrFI RCCGGY 3 cut(s) 86, 195, 516
BssAI RCCGGY 3 cut(s) 86, 195, 516
BssECI CCNNGG 7 cut(s) 66, 67, 123, 153, 357, 632, 696
BssNI GRCGYC 3 cut(s) 330, 623, 638
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 68, 124, 187
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 3 cut(s) 330, 623, 638
BstC8I GCNNGC 6 cut(s) 41, 104, 512, 518, 610, 746
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 1 cut(s) 799
BstFNI CGCG 4 cut(s) 39, 282, 359, 477
BstH2I RGCGCY 1 cut(s) 517
BstHHI GCGC 4 cut(s) 39, 99, 510, 516
BstMAI GTCTC 2 cut(s) 230, 253
BstMCI CGRYCG 2 cut(s) 196, 468
BstMWI GCNNNNNNNGC 5 cut(s) 36, 103, 507, 522, 646
BstNI CCWGG 3 cut(s) 68, 124, 187
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 8 cut(s) 66, 122, 152, 153, 185, 217, 631, 789
BstSLI GKGCMC 2 cut(s) 32, 802
BstUI CGCG 4 cut(s) 39, 282, 359, 477
BstV1I GCAGC 2 cut(s) 697, 728
BstX2I RGATCY 2 cut(s) 48, 366
BstYI RGATCY 2 cut(s) 48, 366
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 3 cut(s) 72, 137, 800
BtgI CCRYGG 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 428
BtsCI GGATG 1 cut(s) 799
Cac8I GCNNGC 6 cut(s) 41, 104, 512, 518, 610, 746
CfoI GCGC 4 cut(s) 39, 99, 510, 516
Cfr10I RCCGGY 3 cut(s) 86, 195, 516
Cfr13I GGNCC 5 cut(s) 70, 136, 361, 798, 799
Cfr42I CCGCGG 1 cut(s) 360
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 4 cut(s) 612, 614, 646, 760
Csp6I GTAC 2 cut(s) 90, 273
CspAI ACCGGT 2 cut(s) 86, 195
CviAII CATG 2 cut(s) 103, 532
CviQI GTAC 2 cut(s) 90, 273
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
EciI GGCGGA 3 cut(s) 52, 204, 232
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 674
Eco88I CYCGRG 1 cut(s) 153
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 70, 798
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 66, 122, 185
EcoT14I CCWWGG 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 2 cut(s) 106, 535
FaiI YATR 2 cut(s) 104, 533
FaqI GGGAC 1 cut(s) 649
FatI CATG 2 cut(s) 102, 531
FauI CCCGC 1 cut(s) 676
FbaI TGATCA 1 cut(s) 312
FblI GTMKAC 2 cut(s) 129, 159
Fnu4HI GCNGC 4 cut(s) 100, 647, 711, 742
FokI GGATG 1 cut(s) 806
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 4 cut(s) 100, 647, 711, 742
GlaI GCGC 4 cut(s) 38, 98, 509, 515
GluI GCNGC 4 cut(s) 100, 647, 711, 742
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 517
HaeIII GGCC 3 cut(s) 72, 137, 800
HapII CCGG 9 cut(s) 87, 154, 196, 218, 370, 443, 517, 632, 791
HgaI GACGC 4 cut(s) 612, 614, 646, 760
HhaI GCGC 4 cut(s) 39, 99, 510, 516
Hin1I GRCGYC 3 cut(s) 330, 623, 638
Hin1II CATG 2 cut(s) 106, 535
Hin6I GCGC 4 cut(s) 37, 97, 508, 514
HinP1I GCGC 4 cut(s) 37, 97, 508, 514
HincII GTYRAC 3 cut(s) 130, 160, 591
HindII GTYRAC 3 cut(s) 130, 160, 591
HinfI GANTC 5 cut(s) 131, 563, 587, 614, 763
HpaII CCGG 9 cut(s) 87, 154, 196, 218, 370, 443, 517, 632, 791
HphI GGTGA 3 cut(s) 278, 430, 460
Hpy166II GTNNAC 5 cut(s) 130, 160, 334, 354, 591
Hpy188III TCNNGA 5 cut(s) 23, 256, 326, 364, 381
Hpy8I GTNNAC 5 cut(s) 130, 160, 334, 354, 591
Hpy99I CGWCG 5 cut(s) 272, 296, 352, 428, 781
HpyAV CCTTC 5 cut(s) 378, 387, 484, 541, 649
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 1 cut(s) 106
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 103, 507, 522, 646
HpySE526I ACGT 1 cut(s) 330
Hsp92I GRCGYC 3 cut(s) 330, 623, 638
Hsp92II CATG 2 cut(s) 106, 535
HspAI GCGC 4 cut(s) 37, 97, 508, 514
KpnI GGTACC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 516
KroNI GCCGGC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 312
KspI CCGCGG 1 cut(s) 360
LmnI GCTCC 7 cut(s) 150, 170, 302, 410, 445, 658, 718
Lsp1109I GCAGC 2 cut(s) 697, 728
LweI GCATC 2 cut(s) 24, 333
MaeII ACGT 1 cut(s) 330
MboII GAAGA 3 cut(s) 16, 196, 772
MflI RGATCY 2 cut(s) 48, 366
MhlI GDGCHC 2 cut(s) 32, 802
MluCI AATT 2 cut(s) 319, 574
MlyI GAGTC 4 cut(s) 125, 557, 596, 623
MmeI TCCRAC 2 cut(s) 388, 800
MroNI GCCGGC 1 cut(s) 516
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 9 cut(s) 87, 154, 196, 218, 370, 443, 517, 632, 791
MspR9I CCNGG 8 cut(s) 68, 124, 154, 155, 187, 219, 633, 791
MvaI CCWGG 3 cut(s) 68, 124, 187
MvnI CGCG 4 cut(s) 39, 282, 359, 477
MwoI GCNNNNNNNGC 5 cut(s) 36, 103, 507, 522, 646
NaeI GCCGGC 1 cut(s) 518
NciI CCSGG 5 cut(s) 154, 155, 219, 633, 791
NgoMIV GCCGGC 1 cut(s) 516
NlaIII CATG 2 cut(s) 106, 535
NlaIV GGNNCC 8 cut(s) 91, 172, 200, 213, 298, 441, 799, 800
NmeAIII GCCGAG 2 cut(s) 113, 610
NspI RCATGY 1 cut(s) 106
PaeI GCATGC 1 cut(s) 106
PasI CCCWGGG 1 cut(s) 67
PcsI WCGNNNNNNNCGW 4 cut(s) 21, 126, 297, 474
PdiI GCCGGC 1 cut(s) 518
PfeI GAWTC 1 cut(s) 763
PinAI ACCGGT 2 cut(s) 86, 195
PkrI GCNGC 4 cut(s) 101, 648, 712, 743
Ple19I CGATCG 1 cut(s) 468
PleI GAGTC 4 cut(s) 125, 557, 595, 622
PpsI GAGTC 4 cut(s) 125, 557, 595, 622
Psp6I CCWGG 3 cut(s) 66, 122, 185
PspGI CCWGG 3 cut(s) 66, 122, 185
PspN4I GGNNCC 8 cut(s) 91, 172, 200, 213, 298, 441, 799, 800
PspOMI GGGCCC 1 cut(s) 798
PspPI GGNCC 5 cut(s) 70, 136, 361, 798, 799
PsuI RGATCY 2 cut(s) 48, 366
PvuI CGATCG 1 cut(s) 468
RsaI GTAC 2 cut(s) 91, 274
RsaNI GTAC 2 cut(s) 90, 273
SacII CCGCGG 1 cut(s) 360
SalI GTCGAC 2 cut(s) 128, 158
SatI GCNGC 4 cut(s) 100, 647, 711, 742
Sau96I GGNCC 5 cut(s) 70, 136, 361, 798, 799
SchI GAGTC 4 cut(s) 125, 557, 596, 623
ScrFI CCNGG 8 cut(s) 68, 124, 154, 155, 187, 219, 633, 791
SduI GDGCHC 2 cut(s) 32, 802
SfaNI GCATC 2 cut(s) 24, 333
Sfr303I CCGCGG 1 cut(s) 360
SgrBI CCGCGG 1 cut(s) 360
SinI GGWCC 1 cut(s) 361
SmaI CCCGGG 1 cut(s) 155
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 319, 574
StyD4I CCNGG 8 cut(s) 66, 122, 152, 153, 185, 217, 631, 789
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 333
TaqI TCGA 5 cut(s) 129, 159, 176, 423, 545
TasI AATT 2 cut(s) 319, 574
TauI GCSGC 2 cut(s) 102, 649
TfiI GAWTC 1 cut(s) 763
TseI GCWGC 2 cut(s) 710, 741
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 5 cut(s) 30, 241, 289, 608, 794
TspMI CCCGGG 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 361
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 574
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 129, 159
ZraI GACGTC 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.