pycom01g10290

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009207
Physical Location & Seq
Forward (+)
11087304 .. 11088116
813 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g10290.1

Sequence Viewer

Length: 813 bp
ATGATGATGAAGAAAGAAAACGGAGACGATCACGTGCACTTCTTGGAGATCAACCTCATCTCCGCCCAGGGCCTCAAGCCTCCCTCCGCGGCCCTCCGCCGCATGCAGACCTACGCCCTCGCCTGGGTCGACTCCGCCCACAAGATCCGCTCCCGGGTCGACAAGATCGGCGGCGAGAACCCGACGTGGAACGATCGGTTTCTCTTCAAGGTCCCGTCCGCATTTCTCTCAAGCGAGACCTCCCAGGTCTCCATCCAGATCTACGCCGTCGGCTGCTTCAGCGATCACCTCGTCGGCACCGTCCGACTTCTCATCAACAACTTTCTCGACGTCGCGTCCAAGGTTCCGTCGTTTACGGCCATCCAGATTCGGAGACCTTCTGGAAGATTCCAAGGCGTATTGAATGTGGCCGCGATGGTGATCGACGGCTCGTACCTGGCCCCGGCTGTGAGCGAGTTGTCTGCGGTCGGGTACCGCGACCTGATGGGAAAGGGAGAAAGCTTCCGGCGCCGGCGACGCGACAGCATGAAGAGCAAATCGAGTGATTACTCCGGCGATTCGAAGGAAAACTCTTTCACTGAGTCGGCGGAGAACTCAGACGTCGGCGGGTCGGCGGCATCATCTCCGGCGACGCCGTTACCGCCTCTGAAGGAGCTGAACACGATCTCCGAATTGGCGGGAACAACAAGGCAGGTGTTGAAGGCTCGTCCAACGGACGGCTCGCGTTTCCTGTGCTGCTTGCTGACGCAGAGGAAGATTCAATACAGTCCGTCGGATGAGAACGCGGATGGGGCCCACAGCAGAGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

271

Amino Acids

29.65

Weight (kDa)

9.25

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 15 - 108 9.7e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 682
AatII GACGTC 2 cut(s) 333, 603
Acc36I ACCTGC 1 cut(s) 682
Acc65I GGTACC 1 cut(s) 471
AccB1I GGYRCC 3 cut(s) 296, 471, 507
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 129, 159
AccII CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
AclWI GGATC 1 cut(s) 139
AcoI YGGCCR 2 cut(s) 357, 408
AcuI CTGAAG 2 cut(s) 262, 668
AcvI CACGTG 1 cut(s) 34
AcyI GRCGYC 4 cut(s) 330, 508, 600, 632
AfaI GTAC 2 cut(s) 434, 473
AfiI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 442, 716
AgsI TTSAA 4 cut(s) 208, 403, 700, 761
AhdI GACNNNNNGTC 1 cut(s) 334
AjiI CACGTC 1 cut(s) 186
AjnI CCWGG 4 cut(s) 66, 122, 243, 435
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 2 cut(s) 501, 655
AluI AGCT 2 cut(s) 501, 655
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 4 cut(s) 18, 230, 253, 367
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 6 cut(s) 70, 90, 357, 408, 438, 792
ApaI GGGCCC 1 cut(s) 796
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 273, 735
Asp718I GGTACC 1 cut(s) 471
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 6 cut(s) 70, 91, 211, 439, 792, 793
AsuC2I CCSGG 3 cut(s) 154, 155, 443
AsuHPI GGTGA 2 cut(s) 278, 430
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 2 cut(s) 39, 796
BanI GGYRCC 3 cut(s) 296, 471, 507
BanII GRGCYC 1 cut(s) 796
BarI GAAGNNNNNNTAC 2 cut(s) 746, 778
BbrPI CACGTG 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 260, 722
BccI CCATC 5 cut(s) 260, 368, 409, 478, 782
BceAI ACGGC 5 cut(s) 251, 372, 442, 619, 733
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BciT130I CCWGG 4 cut(s) 68, 124, 245, 437
BcnI CCSGG 3 cut(s) 154, 155, 443
BcoDI GTCTC 4 cut(s) 18, 230, 253, 367
BfoI RGCGCY 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 682
BglII AGATCT 1 cut(s) 258
BisI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
BlsI GCNGC 7 cut(s) 91, 101, 173, 275, 412, 616, 737
Bme1390I CCNGG 7 cut(s) 68, 124, 154, 155, 245, 437, 443
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 186
BmgT120I GGNCC 6 cut(s) 70, 91, 211, 439, 792, 793
BmiI GGNNCC 8 cut(s) 213, 298, 345, 441, 473, 509, 793, 794
BmrFI CCNGG 7 cut(s) 68, 124, 154, 155, 245, 437, 443
BmsI GCATC 1 cut(s) 626
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 2 cut(s) 59, 214
BpuMI CCSGG 3 cut(s) 154, 155, 443
BsaAI YACGTR 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 419
BsaHI GRCGYC 4 cut(s) 330, 508, 600, 632
BsaI GGTCTC 3 cut(s) 230, 253, 367
BsaJI CCNNGG 9 cut(s) 66, 67, 87, 123, 153, 243, 339, 391, 441
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 650, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 124, 154, 442, 716
Bse118I RCCGGY 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 419
BseBI CCWGG 4 cut(s) 68, 124, 245, 437
BseDI CCNNGG 9 cut(s) 66, 67, 87, 123, 153, 243, 339, 391, 441
BseGI GGATG 4 cut(s) 252, 360, 781, 793
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 442, 716
BseMII CTCAG 2 cut(s) 570, 609
BseSI GKGCMC 2 cut(s) 39, 796
BseXI GCAGC 2 cut(s) 260, 722
Bsh1236I CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
Bsh1285I CGRYCG 2 cut(s) 196, 468
BshFI GGCC 6 cut(s) 72, 92, 359, 410, 440, 794
BshNI GGYRCC 3 cut(s) 296, 471, 507
BsiEI CGRYCG 2 cut(s) 196, 468
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 442, 716
BsmAI GTCTC 4 cut(s) 18, 230, 253, 367
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 6 cut(s) 72, 92, 359, 410, 440, 794
Bso31I GGTCTC 3 cut(s) 230, 253, 367
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 560
Bsp120I GGGCCC 1 cut(s) 792
Bsp1286I GDGCHC 2 cut(s) 39, 796
Bsp143I GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
BspANI GGCC 6 cut(s) 72, 92, 359, 410, 440, 794
BspCNI CTCAG 2 cut(s) 571, 608
BspFNI CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
BspLI GGNNCC 8 cut(s) 213, 298, 345, 441, 473, 509, 793, 794
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 524
BspT104I TTCGAA 1 cut(s) 560
BspT107I GGYRCC 3 cut(s) 296, 471, 507
BspTNI GGTCTC 3 cut(s) 230, 253, 367
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 9 cut(s) 66, 67, 87, 123, 153, 243, 339, 391, 441
BssMI GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
BssNI GRCGYC 4 cut(s) 330, 508, 600, 632
BssT1I CCWWGG 2 cut(s) 339, 391
Bst2UI CCWGG 4 cut(s) 68, 124, 245, 437
Bst4CI ACNGT 2 cut(s) 301, 767
Bst6I CTCTTC 2 cut(s) 209, 524
BstACI GRCGYC 4 cut(s) 330, 508, 600, 632
BstBAI YACGTR 1 cut(s) 34
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 104, 512, 722, 740
BstDEI CTNAG 2 cut(s) 579, 595
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 4 cut(s) 252, 360, 781, 793
BstFNI CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 9 cut(s) 31, 51, 147, 168, 196, 261, 286, 423, 666
BstMAI GTCTC 4 cut(s) 18, 230, 253, 367
BstMBI GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
BstMCI CGRYCG 2 cut(s) 196, 468
BstMWI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
BstNI CCWGG 4 cut(s) 68, 124, 245, 437
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 7 cut(s) 66, 122, 152, 153, 243, 435, 441
BstSLI GKGCMC 2 cut(s) 39, 796
BstUI CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
BstV1I GCAGC 2 cut(s) 260, 722
BstX2I RGATCY 2 cut(s) 144, 258
BstYI RGATCY 2 cut(s) 144, 258
BsuRI GGCC 6 cut(s) 72, 92, 359, 410, 440, 794
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 428
BtrI CACGTC 1 cut(s) 186
BtsCI GGATG 4 cut(s) 252, 360, 781, 793
BtsIMutI CAGTG 1 cut(s) 576
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 4 cut(s) 104, 512, 722, 740
CfoI GCGC 1 cut(s) 510
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 6 cut(s) 70, 91, 211, 439, 792, 793
Cfr42I CCGCGG 1 cut(s) 90
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 4 cut(s) 324, 525, 640, 754
Csp6I GTAC 2 cut(s) 433, 472
CviAII CATG 2 cut(s) 103, 526
CviQI GTAC 2 cut(s) 433, 472
DdeI CTNAG 2 cut(s) 579, 595
DinI GGCGCC 1 cut(s) 509
DpnI GATC 9 cut(s) 30, 50, 146, 167, 195, 260, 285, 422, 665
DpnII GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 357, 408
Eam1104I CTCTTC 2 cut(s) 209, 524
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 2 cut(s) 209, 524
EciI GGCGGA 4 cut(s) 52, 86, 124, 602
Eco130I CCWWGG 2 cut(s) 339, 391
Eco24I GRGCYC 1 cut(s) 796
Eco31I GGTCTC 3 cut(s) 230, 253, 367
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 262, 668
Eco72I CACGTG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 153
EcoO109I RGGNCCY 3 cut(s) 70, 211, 792
EcoRII CCWGG 4 cut(s) 66, 122, 243, 435
EcoT14I CCWWGG 2 cut(s) 339, 391
EcoT38I GRGCYC 1 cut(s) 796
EgeI GGCGCC 1 cut(s) 509
EheI GGCGCC 1 cut(s) 509
ErhI CCWWGG 2 cut(s) 339, 391
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 2 cut(s) 106, 529
FaiI YATR 2 cut(s) 104, 527
FaqI GGGAC 1 cut(s) 197
FatI CATG 2 cut(s) 102, 525
FauI CCCGC 2 cut(s) 599, 670
FblI GTMKAC 2 cut(s) 129, 159
Fnu4HI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
FokI GGATG 4 cut(s) 239, 347, 788, 800
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
GlaI GCGC 1 cut(s) 509
GluI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 6 cut(s) 72, 92, 359, 410, 440, 794
HapII CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
HgaI GACGC 4 cut(s) 324, 525, 640, 754
HhaI GCGC 1 cut(s) 510
Hin1I GRCGYC 4 cut(s) 330, 508, 600, 632
Hin1II CATG 2 cut(s) 106, 529
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HincII GTYRAC 2 cut(s) 130, 160
HindII GTYRAC 2 cut(s) 130, 160
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 6 cut(s) 131, 367, 387, 557, 581, 757
HpaII CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
HphI GGTGA 2 cut(s) 278, 430
Hpy166II GTNNAC 4 cut(s) 37, 130, 160, 354
Hpy188I TCNGA 6 cut(s) 305, 372, 598, 648, 670, 775
Hpy188III TCNNGA 4 cut(s) 256, 326, 364, 381
Hpy8I GTNNAC 4 cut(s) 37, 130, 160, 354
HpyAV CCTTC 4 cut(s) 387, 556, 643, 694
HpyCH4III ACNGT 2 cut(s) 301, 767
HpyCH4IV ACGT 4 cut(s) 33, 185, 330, 600
HpyCH4V TGCA 2 cut(s) 37, 106
HpyF10VI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
HpyF3I CTNAG 2 cut(s) 579, 595
HpySE526I ACGT 4 cut(s) 33, 185, 330, 600
Hsp92I GRCGYC 4 cut(s) 330, 508, 600, 632
Hsp92II CATG 2 cut(s) 106, 529
HspAI GCGC 1 cut(s) 508
KasI GGCGCC 1 cut(s) 507
KpnI GGTACC 1 cut(s) 475
KroI GCCGGC 1 cut(s) 510
KroNI GCCGGC 1 cut(s) 512
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
LguI GCTCTTC 1 cut(s) 524
LmnI GCTCC 2 cut(s) 155, 652
Lsp1109I GCAGC 2 cut(s) 260, 722
LweI GCATC 1 cut(s) 626
MaeII ACGT 4 cut(s) 33, 185, 330, 600
MaeIII GTNAC 1 cut(s) 636
MalI GATC 9 cut(s) 30, 50, 146, 167, 195, 260, 285, 422, 665
MbiI CCGCTC 1 cut(s) 150
MboI GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
MboII GAAGA 5 cut(s) 22, 196, 396, 541, 766
MflI RGATCY 2 cut(s) 144, 258
MhlI GDGCHC 2 cut(s) 39, 796
MluCI AATT 1 cut(s) 671
Mly113I GGCGCC 1 cut(s) 508
MlyI GAGTC 2 cut(s) 125, 590
MmeI TCCRAC 3 cut(s) 328, 734, 753
MreI CGCCGGCG 1 cut(s) 510
MroNI GCCGGC 1 cut(s) 510
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
MspR9I CCNGG 7 cut(s) 68, 124, 154, 155, 245, 437, 443
MvaI CCWGG 4 cut(s) 68, 124, 245, 437
MvnI CGCG 7 cut(s) 89, 335, 413, 477, 519, 724, 785
MwoI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
NaeI GCCGGC 1 cut(s) 512
NarI GGCGCC 1 cut(s) 508
NciI CCSGG 3 cut(s) 154, 155, 443
NdeII GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
NgoMIV GCCGGC 1 cut(s) 510
NlaIII CATG 2 cut(s) 106, 529
NlaIV GGNNCC 8 cut(s) 213, 298, 345, 441, 473, 509, 793, 794
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 560
PaeI GCATGC 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 682
PasI CCCWGGG 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 524
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 297, 474
PdiI GCCGGC 1 cut(s) 512
PfeI GAWTC 4 cut(s) 367, 387, 557, 757
PkrI GCNGC 7 cut(s) 91, 101, 173, 275, 412, 616, 737
Ple19I CGATCG 1 cut(s) 196
PleI GAGTC 2 cut(s) 125, 589
PluTI GGCGCC 1 cut(s) 511
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 2 cut(s) 125, 589
Ppu21I YACGTR 1 cut(s) 34
PpuMI RGGWCCY 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 4 cut(s) 66, 122, 243, 435
PspCI CACGTG 1 cut(s) 34
PspGI CCWGG 4 cut(s) 66, 122, 243, 435
PspN4I GGNNCC 8 cut(s) 213, 298, 345, 441, 473, 509, 793, 794
PspOMI GGGCCC 1 cut(s) 792
PspPI GGNCC 6 cut(s) 70, 91, 211, 439, 792, 793
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 2 cut(s) 144, 258
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 2 cut(s) 434, 473
RsaNI GTAC 2 cut(s) 433, 472
SacII CCGCGG 1 cut(s) 90
SalI GTCGAC 2 cut(s) 128, 158
SapI GCTCTTC 1 cut(s) 524
SatI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
Sau3AI GATC 9 cut(s) 28, 48, 144, 165, 193, 258, 283, 420, 663
Sau96I GGNCC 6 cut(s) 70, 91, 211, 439, 792, 793
SchI GAGTC 2 cut(s) 125, 590
ScrFI CCNGG 7 cut(s) 68, 124, 154, 155, 245, 437, 443
SduI GDGCHC 2 cut(s) 39, 796
SfaNI GCATC 1 cut(s) 626
SfoI GGCGCC 1 cut(s) 509
Sfr303I CCGCGG 1 cut(s) 90
SfuI TTCGAA 1 cut(s) 560
SgrAI CRCCGGYG 1 cut(s) 510
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 1 cut(s) 211
SmaI CCCGGG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 74, 229
SmoI CTYRAG 2 cut(s) 74, 229
SphI GCATGC 1 cut(s) 106
Sse9I AATT 1 cut(s) 671
SspDI GGCGCC 1 cut(s) 507
StyD4I CCNGG 7 cut(s) 66, 122, 152, 153, 243, 435, 441
StyI CCWWGG 2 cut(s) 339, 391
TaaI ACNGT 2 cut(s) 301, 767
TaiI ACGT 4 cut(s) 36, 188, 333, 603
TaqI TCGA 6 cut(s) 129, 159, 327, 423, 539, 560
TasI AATT 1 cut(s) 671
TauI GCSGC 5 cut(s) 92, 102, 174, 413, 617
TfiI GAWTC 4 cut(s) 367, 387, 557, 757
TscAI CASTG 1 cut(s) 583
TseI GCWGC 2 cut(s) 273, 735
TspDTI ATGAA 2 cut(s) 23, 542
TspGWI ACGGA 4 cut(s) 36, 336, 728, 759
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 211
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 129, 159
ZraI GACGTC 2 cut(s) 331, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.