Rh1DG271200

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0009207
Physical Location & Seq
Forward (+)
49128375 .. 49129825
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG271200.1

Sequence Viewer

Length: 822 bp
ATGAAGAGAATAGGAGGAGTAGCCGAGAAAATGCACGTGCTGGAGATCAACCTGATCTCCGGTCAGGGCTTGAAAGTCCCGTCCGGGAAGTTACGGCGGATGAAGACCTACGCAATCGCCTGGGTCGACTCGGACCACAAGATTCGGACCCGGGTCGACAAAGTCGGAGCCGAGAACCCGACTTGGAACGACCGGTTCCTCTTCAAGGTCTCCTCCGAATTCCTCGCCAGGGAGACGTCCGGGATCACGATCGAGATCTACGCCGTCGGTGTCATCCGCGACCATCTCGTCGGCACCGTCCGATTGATCCTCAGCAACTTTCTTGACCTGGAATCCAAGATCCCGTCGTTCACGGCGCTGCAGATTCGCCGGCCTTCTGGAAGATTCCACGGCGTGTTGAACGTCGCCGCGTTGGTCAATCACGAGTCGGAGTGGTCCAAGGAGCTCGACGACGTCTCGGCGATCGGGTACCAAGATCTGATGGGCAAGGGAGAGAGCTTCCGGCGCCGGCGCCGGAGGGACAGCTTGACGAGGTCGACGGAGAACTTCTCCGGCAGCGAGTCGAGGGAGAATTCTTGCGCGGAGTCGGTGGAGAACTCCGAATGCGGCGACTCGTTCAAATCGGCGTCTCCGCCGTCTAAACCGACGTCTCCGGCGGCGGAGAAGAGAGCGCTGAAGGAGGCGAACGGCGTGAGGGATCTAGCGGCCGGAACCAGGGCTCTGAAGACAGCTGTCCCATCGGACGGCTCGCGATTCTTGTGCTGCTTGCTCACGACGCAGAGGAAGGTTCAGTCATCGTCGGATAAGAACGCAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

273

Amino Acids

30.23

Weight (kDa)

9.58

Isoelectric Point (pI)

55.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 13 - 111 8.1e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 287
AatII GACGTC 3 cut(s) 239, 456, 650
Acc65I GGTACC 1 cut(s) 468
AccB1I GGYRCC 4 cut(s) 293, 468, 504, 510
AccI GTMKAC 3 cut(s) 126, 156, 536
AccII CGCG 4 cut(s) 279, 410, 581, 751
AciI CCGC 9 cut(s) 97, 277, 408, 581, 606, 632, 656, 659, 704
AclWI GGATC 4 cut(s) 251, 301, 334, 705
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 2 cut(s) 218, 571
AcuI CTGAAG 2 cut(s) 695, 743
AcvI CACGTG 1 cut(s) 37
AcyI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
AdeI CACNNNGTG 1 cut(s) 394
AfaI GTAC 1 cut(s) 470
AfeI AGCGCT 1 cut(s) 672
AfiI CCNNNNNNNGG 4 cut(s) 205, 229, 714, 743
AgeI ACCGGT 1 cut(s) 192
AgsI TTSAA 4 cut(s) 73, 205, 400, 619
AjnI CCWGG 4 cut(s) 119, 227, 327, 713
AluBI AGCT 4 cut(s) 445, 498, 525, 731
AluI AGCT 4 cut(s) 445, 498, 525, 731
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 5 cut(s) 214, 227, 460, 633, 654
AlwI GGATC 4 cut(s) 251, 301, 334, 705
Ama87I CYCGRG 1 cut(s) 150
Aor51HI AGCGCT 1 cut(s) 672
AoxI GGCC 2 cut(s) 371, 705
ApeKI GCWGC 3 cut(s) 358, 555, 762
ApoI RAATTY 2 cut(s) 218, 571
AsiGI ACCGGT 1 cut(s) 192
Asp718I GGTACC 1 cut(s) 468
AspLEI GCGC 5 cut(s) 358, 507, 513, 581, 673
AspS9I GGNCC 3 cut(s) 133, 147, 435
AsuC2I CCSGG 4 cut(s) 85, 151, 152, 241
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 3 cut(s) 133, 147, 435
BanI GGYRCC 4 cut(s) 293, 468, 504, 510
BanII GRGCYC 2 cut(s) 447, 721
BauI CACGAG 1 cut(s) 422
BbrPI CACGTG 1 cut(s) 37
BbsI GAAGAC 2 cut(s) 110, 731
Bbv12I GWGCWC 1 cut(s) 447
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 3 cut(s) 345, 567, 749
BccI CCATC 3 cut(s) 291, 475, 745
BceAI ACGGC 7 cut(s) 110, 248, 369, 406, 619, 703, 760
BcgI CGANNNNNNTGC 2 cut(s) 741, 775
BciT130I CCWGG 4 cut(s) 121, 229, 329, 715
BcnI CCSGG 4 cut(s) 85, 151, 152, 241
BcoDI GTCTC 5 cut(s) 214, 227, 460, 633, 654
BfaI CTAG 1 cut(s) 701
BfmI CTRYAG 1 cut(s) 359
BfoI RGCGCY 4 cut(s) 359, 508, 514, 674
BglII AGATCT 2 cut(s) 255, 475
BisI GCNGC 7 cut(s) 359, 408, 556, 607, 657, 705, 763
BlsI GCNGC 7 cut(s) 360, 409, 557, 608, 658, 706, 764
Bme1390I CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 715
Bme18I GGWCC 3 cut(s) 133, 147, 435
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 133, 147, 435
BmiI GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 712
BmrFI CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 715
BoxI GACNNNNGTC 2 cut(s) 152, 731
BpiI GAAGAC 2 cut(s) 110, 731
BplI GAGNNNNNCTC 2 cut(s) 533, 565
BpmI CTGGAG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 311
BpuMI CCSGG 4 cut(s) 85, 151, 152, 241
BsaAI YACGTR 1 cut(s) 37
BsaBI GATNNNNATC 2 cut(s) 248, 254
BsaHI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 714
BsaWI WCCGGW 2 cut(s) 59, 192
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 229, 714, 743
Bse118I RCCGGY 3 cut(s) 192, 369, 507
Bse8I GATNNNNATC 2 cut(s) 248, 254
BseBI CCWGG 4 cut(s) 121, 229, 329, 715
BseDI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 714
BseGI GGATG 2 cut(s) 105, 273
BseJI GATNNNNATC 2 cut(s) 248, 254
BseLI CCNNNNNNNGG 4 cut(s) 205, 229, 714, 743
BseMII CTCAG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 30, 202
BseX3I CGGCCG 1 cut(s) 705
BseXI GCAGC 3 cut(s) 345, 567, 749
Bsh1236I CGCG 4 cut(s) 279, 410, 581, 751
Bsh1285I CGRYCG 4 cut(s) 193, 252, 465, 708
BshFI GGCC 2 cut(s) 373, 707
BshNI GGYRCC 4 cut(s) 293, 468, 504, 510
BshTI ACCGGT 1 cut(s) 192
BsiEI CGRYCG 4 cut(s) 193, 252, 465, 708
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 150
BslFI GGGAC 3 cut(s) 62, 533, 719
BslI CCNNNNNNNGG 4 cut(s) 205, 229, 714, 743
BsmAI GTCTC 5 cut(s) 214, 227, 460, 633, 654
BsmBI CGTCTC 4 cut(s) 227, 460, 633, 654
BsmFI GGGAC 3 cut(s) 62, 533, 719
BsmI GAATGC 1 cut(s) 608
BsnI GGCC 2 cut(s) 373, 707
Bso31I GGTCTC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 447, 721
Bsp68I TCGCGA 1 cut(s) 751
BspACI CCGC 9 cut(s) 97, 277, 408, 581, 606, 632, 656, 659, 704
BspANI GGCC 2 cut(s) 373, 707
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 4 cut(s) 279, 410, 581, 751
BspLI GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 712
BspMAI CTGCAG 1 cut(s) 363
BspPI GGATC 4 cut(s) 251, 301, 334, 705
BspT107I GGYRCC 4 cut(s) 293, 468, 504, 510
BspTNI GGTCTC 1 cut(s) 214
BsrFI RCCGGY 3 cut(s) 192, 369, 507
BssAI RCCGGY 3 cut(s) 192, 369, 507
BssECI CCNNGG 6 cut(s) 120, 150, 228, 388, 438, 714
BssNI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
BssSI CACGAG 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 438
Bst2BI CACGAG 1 cut(s) 422
Bst2UI CCWGG 4 cut(s) 121, 229, 329, 715
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 2 cut(s) 206, 659
BstACI GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 4 cut(s) 371, 509, 749, 767
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 1 cut(s) 388
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 105, 273
BstFNI CGCG 4 cut(s) 279, 410, 581, 751
BstH2I RGCGCY 4 cut(s) 359, 508, 514, 674
BstHHI GCGC 5 cut(s) 358, 507, 513, 581, 673
BstMAI GTCTC 5 cut(s) 214, 227, 460, 633, 654
BstMCI CGRYCG 4 cut(s) 193, 252, 465, 708
BstMWI GCNNNNNNNGC 2 cut(s) 504, 775
BstNI CCWGG 4 cut(s) 121, 229, 329, 715
BstPAI GACNNNNGTC 2 cut(s) 152, 731
BstSCI CCNGG 8 cut(s) 83, 119, 149, 150, 227, 239, 327, 713
BstSFI CTRYAG 1 cut(s) 359
BstUI CGCG 4 cut(s) 279, 410, 581, 751
BstV1I GCAGC 3 cut(s) 345, 567, 749
BstV2I GAAGAC 2 cut(s) 110, 731
BstX2I RGATCY 4 cut(s) 255, 339, 475, 697
BstYI RGATCY 4 cut(s) 255, 339, 475, 697
BstZI CGGCCG 1 cut(s) 705
BsuRI GGCC 2 cut(s) 373, 707
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 105, 273
BtuMI TCGCGA 1 cut(s) 751
Cac8I GCNNGC 4 cut(s) 371, 509, 749, 767
CfoI GCGC 5 cut(s) 358, 507, 513, 581, 673
Cfr10I RCCGGY 3 cut(s) 192, 369, 507
Cfr13I GGNCC 3 cut(s) 133, 147, 435
Cfr9I CCCGGG 1 cut(s) 150
CseI GACGC 2 cut(s) 615, 784
Csp6I GTAC 1 cut(s) 469
CspAI ACCGGT 1 cut(s) 192
CviQI GTAC 1 cut(s) 469
DdeI CTNAG 1 cut(s) 311
DinI GGCGCC 2 cut(s) 506, 512
DraIII CACNNNGTG 1 cut(s) 394
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 705
EagI CGGCCG 1 cut(s) 705
Eam1104I CTCTTC 2 cut(s) 206, 659
EarI CTCTTC 2 cut(s) 206, 659
EciI GGCGGA 3 cut(s) 112, 621, 674
Ecl136II GAGCTC 1 cut(s) 445
EclXI CGGCCG 1 cut(s) 705
Eco130I CCWWGG 1 cut(s) 438
Eco24I GRGCYC 2 cut(s) 447, 721
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 3 cut(s) 133, 147, 435
Eco47III AGCGCT 1 cut(s) 672
Eco52I CGGCCG 1 cut(s) 705
Eco53kI GAGCTC 1 cut(s) 445
Eco57I CTGAAG 2 cut(s) 695, 743
Eco72I CACGTG 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 445
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRI GAATTC 2 cut(s) 218, 571
EcoRII CCWGG 4 cut(s) 119, 227, 327, 713
EcoT14I CCWWGG 1 cut(s) 438
EcoT38I GRGCYC 2 cut(s) 447, 721
EgeI GGCGCC 2 cut(s) 506, 512
EheI GGCGCC 2 cut(s) 506, 512
ErhI CCWWGG 1 cut(s) 438
Esp3I CGTCTC 4 cut(s) 227, 460, 633, 654
FaqI GGGAC 3 cut(s) 62, 533, 719
FblI GTMKAC 3 cut(s) 126, 156, 536
Fnu4HI GCNGC 7 cut(s) 359, 408, 556, 607, 657, 705, 763
FokI GGATG 2 cut(s) 112, 260
FriOI GRGCYC 2 cut(s) 447, 721
Fsp4HI GCNGC 7 cut(s) 359, 408, 556, 607, 657, 705, 763
FspBI CTAG 1 cut(s) 701
GlaI GCGC 5 cut(s) 357, 506, 512, 580, 672
GluI GCNGC 7 cut(s) 359, 408, 556, 607, 657, 705, 763
GsuI CTGGAG 1 cut(s) 62
HaeII RGCGCY 4 cut(s) 359, 508, 514, 674
HaeIII GGCC 2 cut(s) 373, 707
HgaI GACGC 2 cut(s) 615, 784
HhaI GCGC 5 cut(s) 358, 507, 513, 581, 673
Hin1I GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
Hin6I GCGC 5 cut(s) 356, 505, 511, 579, 671
HinP1I GCGC 5 cut(s) 356, 505, 511, 579, 671
HincII GTYRAC 3 cut(s) 127, 157, 537
HindII GTYRAC 3 cut(s) 127, 157, 537
Hpy166II GTNNAC 4 cut(s) 127, 157, 351, 537
Hpy188III TCNNGA 7 cut(s) 247, 253, 323, 378, 422, 750, 772
Hpy8I GTNNAC 4 cut(s) 127, 157, 351, 537
HpyAV CCTTC 3 cut(s) 384, 670, 778
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 5 cut(s) 36, 236, 402, 453, 647
HpyCH4V TGCA 2 cut(s) 34, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 504, 775
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 5 cut(s) 36, 236, 402, 453, 647
Hsp92I GRCGYC 6 cut(s) 236, 453, 505, 511, 626, 647
HspAI GCGC 5 cut(s) 356, 505, 511, 579, 671
KasI GGCGCC 2 cut(s) 504, 510
KpnI GGTACC 1 cut(s) 472
KroI GCCGGC 2 cut(s) 369, 507
KroNI GCCGGC 2 cut(s) 371, 509
LmnI GCTCC 2 cut(s) 167, 442
Lsp1109I GCAGC 3 cut(s) 345, 567, 749
MaeI CTAG 1 cut(s) 701
MaeII ACGT 5 cut(s) 36, 236, 402, 453, 647
MaeIII GTNAC 1 cut(s) 90
MboII GAAGA 6 cut(s) 16, 115, 193, 393, 676, 736
MflI RGATCY 4 cut(s) 255, 339, 475, 697
MhlI GDGCHC 2 cut(s) 447, 721
MluCI AATT 2 cut(s) 218, 571
Mly113I GGCGCC 2 cut(s) 505, 511
MlyI GAGTC 5 cut(s) 122, 434, 569, 593, 605
MmeI TCCRAC 3 cut(s) 145, 408, 780
MreI CGCCGGCG 1 cut(s) 507
MroNI GCCGGC 2 cut(s) 369, 507
MspA1I CMGCKG 1 cut(s) 731
MspR9I CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 715
Mva1269I GAATGC 1 cut(s) 608
MvaI CCWGG 4 cut(s) 121, 229, 329, 715
MvnI CGCG 4 cut(s) 279, 410, 581, 751
MwoI GCNNNNNNNGC 2 cut(s) 504, 775
NaeI GCCGGC 2 cut(s) 371, 509
NarI GGCGCC 2 cut(s) 505, 511
NciI CCSGG 4 cut(s) 85, 151, 152, 241
NgoMIV GCCGGC 2 cut(s) 369, 507
NlaIV GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 712
NmeAIII GCCGAG 3 cut(s) 49, 196, 437
NruI TCGCGA 1 cut(s) 751
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 294
PctI GAATGC 1 cut(s) 608
PdiI GCCGGC 2 cut(s) 371, 509
PfeI GAWTC 5 cut(s) 142, 332, 364, 384, 753
PflFI GACNNNGTC 3 cut(s) 161, 452, 532
PfoI TCCNGGA 2 cut(s) 83, 239
PinAI ACCGGT 1 cut(s) 192
PkrI GCNGC 7 cut(s) 360, 409, 557, 608, 658, 706, 764
Ple19I CGATCG 2 cut(s) 252, 465
PleI GAGTC 5 cut(s) 122, 433, 568, 592, 605
PluTI GGCGCC 2 cut(s) 508, 514
PmaCI CACGTG 1 cut(s) 37
PmlI CACGTG 1 cut(s) 37
PpsI GAGTC 5 cut(s) 122, 433, 568, 592, 605
Ppu21I YACGTR 1 cut(s) 37
PshAI GACNNNNGTC 2 cut(s) 152, 731
Psp124BI GAGCTC 1 cut(s) 447
Psp6I CCWGG 4 cut(s) 119, 227, 327, 713
PspCI CACGTG 1 cut(s) 37
PspGI CCWGG 4 cut(s) 119, 227, 327, 713
PspN4I GGNNCC 8 cut(s) 149, 169, 197, 295, 470, 506, 512, 712
PspPI GGNCC 3 cut(s) 133, 147, 435
PstI CTGCAG 1 cut(s) 363
PsuI RGATCY 4 cut(s) 255, 339, 475, 697
PsyI GACNNNGTC 3 cut(s) 161, 452, 532
PvuI CGATCG 2 cut(s) 252, 465
PvuII CAGCTG 1 cut(s) 731
RruI TCGCGA 1 cut(s) 751
RsaI GTAC 1 cut(s) 470
RsaNI GTAC 1 cut(s) 469
SacI GAGCTC 1 cut(s) 447
SalI GTCGAC 3 cut(s) 125, 155, 535
SatI GCNGC 7 cut(s) 359, 408, 556, 607, 657, 705, 763
Sau96I GGNCC 3 cut(s) 133, 147, 435
SchI GAGTC 5 cut(s) 122, 434, 569, 593, 605
ScrFI CCNGG 8 cut(s) 85, 121, 151, 152, 229, 241, 329, 715
SduI GDGCHC 2 cut(s) 447, 721
SfcI CTRYAG 1 cut(s) 359
SfoI GGCGCC 2 cut(s) 506, 512
SgrAI CRCCGGYG 1 cut(s) 507
SinI GGWCC 3 cut(s) 133, 147, 435
SmaI CCCGGG 1 cut(s) 152
Sse9I AATT 2 cut(s) 218, 571
SsiI CCGC 9 cut(s) 97, 277, 408, 581, 606, 632, 656, 659, 704
SspDI GGCGCC 2 cut(s) 504, 510
SspMI CTAG 1 cut(s) 701
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 8 cut(s) 83, 119, 149, 150, 227, 239, 327, 713
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 5 cut(s) 39, 239, 405, 456, 650
TaqI TCGA 6 cut(s) 126, 156, 252, 447, 536, 563
TasI AATT 2 cut(s) 218, 571
TauI GCSGC 4 cut(s) 410, 609, 659, 707
TfiI GAWTC 5 cut(s) 142, 332, 364, 384, 753
TseI GCWGC 3 cut(s) 358, 555, 762
TspDTI ATGAA 2 cut(s) 17, 116
TspGWI ACGGA 1 cut(s) 554
TspMI CCCGGG 1 cut(s) 150
Tth111I GACNNNGTC 3 cut(s) 161, 452, 532
VpaK11BI GGWCC 3 cut(s) 133, 147, 435
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 2 cut(s) 218, 571
XmaI CCCGGG 1 cut(s) 150
XmiI GTMKAC 3 cut(s) 126, 156, 536
XspI CTAG 1 cut(s) 701
ZraI GACGTC 3 cut(s) 237, 454, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.