MD03G1187600.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009207
Physical Location & Seq
Reverse (-)
25653961 .. 25654425
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1187600.v1.1.491

Sequence Viewer

Length: 465 bp
ATGCAAGGACTCCATCACCAACAGCAGCAACTCGCGGCGTTTCTCTCCGTCGCCATCCCCAAGGACGATTCCGCCCTCGCCTGGGTCGACTCCGCCCACAAGATCCGCTCCCGGGTCGACAAGATCGGCGGCGAGAACCCCACGTGGAACGATCGGTTCCTCTTCAAGGTCCCGTCCGCATTTCTCTCAAGAGAGACCTCTCATGTCTCCATCCAGATCTACGCCGTCGGCTGCTTCAGTGATCACCTCGTCAGCACCGTCCGACTTCTGATCAACAACTTTCTCGACGTCCCGTCCAAGGTTCCGTCGGCTGCTTCCGCGAGACCTTCTGGAAGATTCCAAGGCGTGTTGAATGTGGCCGCGATGGTGATCGACGGCTCGTATCTGGCTCCGGCGGTGAGCGAGTTGTCTGCGGTCGGGTACCGCGACCTGATGGGAAAGGGAGAAAGCTTCCGGTGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

155

Amino Acids

16.82

Weight (kDa)

9.06

Isoelectric Point (pI)

26.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
Acc65I GGTACC 1 cut(s) 420
AccB1I GGYRCC 2 cut(s) 420, 456
AccBSI CCGCTC 1 cut(s) 108
AccI GTMKAC 2 cut(s) 87, 117
AccII CGCG 4 cut(s) 35, 320, 362, 426
AclWI GGATC 1 cut(s) 97
AcoI YGGCCR 1 cut(s) 357
AcuI CTGAAG 1 cut(s) 220
AcvI CACGTG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 5 cut(s) 81, 82, 112, 166, 298
AgsI TTSAA 2 cut(s) 166, 352
AhdI GACNNNNNGTC 1 cut(s) 292
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 1 cut(s) 450
AluI AGCT 1 cut(s) 450
Alw26I GTCTC 3 cut(s) 188, 211, 316
AlwI GGATC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 111
AoxI GGCC 1 cut(s) 357
ApeKI GCWGC 3 cut(s) 25, 231, 311
Asp718I GGTACC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 169
AsuC2I CCSGG 2 cut(s) 112, 113
AsuHPI GGTGA 4 cut(s) 8, 236, 379, 409
AvaI CYCGRG 1 cut(s) 111
AvaII GGWCC 1 cut(s) 169
BanI GGYRCC 2 cut(s) 420, 456
BbrPI CACGTG 1 cut(s) 144
BbvI GCAGC 3 cut(s) 37, 218, 298
BccI CCATC 5 cut(s) 21, 62, 218, 358, 427
BceAI ACGGC 2 cut(s) 209, 391
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 2 cut(s) 241, 270
BcnI CCSGG 2 cut(s) 112, 113
BcoDI GTCTC 3 cut(s) 188, 211, 316
BglII AGATCT 1 cut(s) 216
BisI GCNGC 6 cut(s) 26, 36, 130, 232, 312, 360
BlsI GCNGC 6 cut(s) 27, 37, 131, 233, 313, 361
Bme1390I CCNGG 3 cut(s) 82, 112, 113
Bme18I GGWCC 1 cut(s) 169
BmeRI GACNNNNNGTC 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 169
BmiI GGNNCC 6 cut(s) 158, 171, 303, 390, 422, 458
BmrFI CCNGG 3 cut(s) 82, 112, 113
BpuEI CTTGAG 1 cut(s) 172
BpuMI CCSGG 2 cut(s) 112, 113
BsaAI YACGTR 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 2 cut(s) 188, 316
BsaJI CCNNGG 5 cut(s) 60, 81, 111, 297, 340
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 82, 112, 166, 298
Bse118I RCCGGY 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 368
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 5 cut(s) 60, 81, 111, 297, 340
BseGI GGATG 2 cut(s) 54, 210
BseJI GATNNNNATC 1 cut(s) 368
BseLI CCNNNNNNNGG 5 cut(s) 81, 82, 112, 166, 298
BseXI GCAGC 3 cut(s) 37, 218, 298
Bsh1236I CGCG 4 cut(s) 35, 320, 362, 426
Bsh1285I CGRYCG 2 cut(s) 154, 417
BshFI GGCC 1 cut(s) 359
BshNI GGYRCC 2 cut(s) 420, 456
BsiEI CGRYCG 2 cut(s) 154, 417
BsiHKCI CYCGRG 1 cut(s) 111
BsiSI CCGG 4 cut(s) 112, 392, 454, 460
BslFI GGGAC 2 cut(s) 155, 275
BslI CCNNNNNNNGG 5 cut(s) 81, 82, 112, 166, 298
BsmAI GTCTC 3 cut(s) 188, 211, 316
BsmFI GGGAC 2 cut(s) 155, 275
BsnI GGCC 1 cut(s) 359
Bso31I GGTCTC 2 cut(s) 188, 316
BsoBI CYCGRG 1 cut(s) 111
Bsp143I GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
BspANI GGCC 1 cut(s) 359
BspFNI CGCG 4 cut(s) 35, 320, 362, 426
BspLI GGNNCC 6 cut(s) 158, 171, 303, 390, 422, 458
BspPI GGATC 1 cut(s) 97
BspT107I GGYRCC 2 cut(s) 420, 456
BspTNI GGTCTC 2 cut(s) 188, 316
BsrBI CCGCTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 459
BssAI RCCGGY 1 cut(s) 459
BssECI CCNNGG 5 cut(s) 60, 81, 111, 297, 340
BssMI GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 3 cut(s) 60, 297, 340
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 144
BstENI CCTNNNNNAGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 54, 210
BstFNI CGCG 4 cut(s) 35, 320, 362, 426
BstKTI GATC 7 cut(s) 105, 126, 154, 219, 244, 273, 372
BstMAI GTCTC 3 cut(s) 188, 211, 316
BstMBI GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
BstMCI CGRYCG 2 cut(s) 154, 417
BstMWI GCNNNNNNNGC 2 cut(s) 317, 456
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 3 cut(s) 80, 110, 111
BstUI CGCG 4 cut(s) 35, 320, 362, 426
BstV1I GCAGC 3 cut(s) 37, 218, 298
BstX2I RGATCY 2 cut(s) 102, 216
BstYI RGATCY 2 cut(s) 102, 216
BsuRI GGCC 1 cut(s) 359
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 2 cut(s) 54, 210
BtsIMutI CAGTG 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 169
Cfr9I CCCGGG 1 cut(s) 111
Csp6I GTAC 1 cut(s) 421
CviAII CATG 1 cut(s) 203
CviJI RGCY 6 cut(s) 231, 311, 359, 378, 389, 450
CviKI_1 RGCY 6 cut(s) 231, 311, 359, 378, 389, 450
CviQI GTAC 1 cut(s) 421
DpnI GATC 7 cut(s) 104, 125, 153, 218, 243, 272, 371
DpnII GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
DriI GACNNNNNGTC 1 cut(s) 292
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 167
Eam1105I GACNNNNNGTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 2 cut(s) 61, 82
Eco130I CCWWGG 3 cut(s) 60, 297, 340
Eco31I GGTCTC 2 cut(s) 188, 316
Eco47I GGWCC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 220
Eco72I CACGTG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 111
EcoNI CCTNNNNNAGG 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 3 cut(s) 60, 297, 340
ErhI CCWWGG 3 cut(s) 60, 297, 340
FaeI CATG 1 cut(s) 206
FaiI YATR 1 cut(s) 204
FaqI GGGAC 2 cut(s) 155, 275
FatI CATG 1 cut(s) 202
FbaI TGATCA 2 cut(s) 241, 270
FblI GTMKAC 2 cut(s) 87, 117
Fnu4HI GCNGC 6 cut(s) 26, 36, 130, 232, 312, 360
FokI GGATG 2 cut(s) 41, 197
Fsp4HI GCNGC 6 cut(s) 26, 36, 130, 232, 312, 360
GluI GCNGC 6 cut(s) 26, 36, 130, 232, 312, 360
HaeIII GGCC 1 cut(s) 359
HapII CCGG 4 cut(s) 112, 392, 454, 460
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 1 cut(s) 206
HincII GTYRAC 2 cut(s) 88, 118
HindII GTYRAC 2 cut(s) 88, 118
HindIII AAGCTT 1 cut(s) 448
HinfI GANTC 4 cut(s) 9, 68, 89, 336
HpaII CCGG 4 cut(s) 112, 392, 454, 460
HphI GGTGA 4 cut(s) 8, 236, 379, 409
Hpy166II GTNNAC 2 cut(s) 88, 118
Hpy188I TCNGA 2 cut(s) 263, 270
Hpy188III TCNNGA 4 cut(s) 189, 214, 284, 330
Hpy8I GTNNAC 2 cut(s) 88, 118
Hpy99I CGWCG 5 cut(s) 53, 230, 290, 310, 377
HpyAV CCTTC 1 cut(s) 336
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 2 cut(s) 143, 288
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 456
HpySE526I ACGT 2 cut(s) 143, 288
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 1 cut(s) 206
KpnI GGTACC 1 cut(s) 424
Ksp22I TGATCA 2 cut(s) 241, 270
Kzo9I GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
LmnI GCTCC 2 cut(s) 113, 394
LpnPI CCDG 8 cut(s) 67, 94, 125, 227, 315, 371, 405, 443
Lsp1109I GCAGC 3 cut(s) 37, 218, 298
MaeII ACGT 2 cut(s) 143, 288
MalI GATC 7 cut(s) 104, 125, 153, 218, 243, 272, 371
MbiI CCGCTC 1 cut(s) 108
MboI GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
MboII GAAGA 2 cut(s) 154, 345
MflI RGATCY 2 cut(s) 102, 216
MlyI GAGTC 2 cut(s) 3, 83
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 4 cut(s) 86, 170, 208, 257
MspI CCGG 4 cut(s) 112, 392, 454, 460
MspR9I CCNGG 3 cut(s) 82, 112, 113
MvaI CCWGG 1 cut(s) 82
MvnI CGCG 4 cut(s) 35, 320, 362, 426
MwoI GCNNNNNNNGC 2 cut(s) 317, 456
NciI CCSGG 2 cut(s) 112, 113
NdeII GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 6 cut(s) 158, 171, 303, 390, 422, 458
PcsI WCGNNNNNNNCGW 3 cut(s) 84, 255, 423
PfeI GAWTC 2 cut(s) 68, 336
PkrI GCNGC 6 cut(s) 27, 37, 131, 233, 313, 361
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 2 cut(s) 3, 83
PmaCI CACGTG 1 cut(s) 144
PmlI CACGTG 1 cut(s) 144
PpsI GAGTC 2 cut(s) 3, 83
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 169
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 80
PspCI CACGTG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 6 cut(s) 158, 171, 303, 390, 422, 458
PspPI GGNCC 1 cut(s) 169
PspPPI RGGWCCY 1 cut(s) 169
PsuI RGATCY 2 cut(s) 102, 216
PvuI CGATCG 1 cut(s) 154
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SalI GTCGAC 2 cut(s) 86, 116
SatI GCNGC 6 cut(s) 26, 36, 130, 232, 312, 360
Sau3AI GATC 7 cut(s) 102, 123, 151, 216, 241, 270, 369
Sau96I GGNCC 1 cut(s) 169
SchI GAGTC 2 cut(s) 3, 83
ScrFI CCNGG 3 cut(s) 82, 112, 113
SetI ASST 9 cut(s) 146, 171, 200, 249, 291, 303, 328, 432, 452
SinI GGWCC 1 cut(s) 169
SmaI CCCGGG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 187
SmoI CTYRAG 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 80, 110, 111
StyI CCWWGG 3 cut(s) 60, 297, 340
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 2 cut(s) 146, 291
TaqI TCGA 4 cut(s) 87, 117, 285, 372
TauI GCSGC 3 cut(s) 38, 132, 362
TfiI GAWTC 2 cut(s) 68, 336
TscAI CASTG 1 cut(s) 244
TseI GCWGC 3 cut(s) 25, 231, 311
TspGWI ACGGA 2 cut(s) 37, 294
TspMI CCCGGG 1 cut(s) 111
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 169
XagI CCTNNNNNAGG 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 111
XmiI GTMKAC 2 cut(s) 87, 117
ZraI GACGTC 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.