Rw1G024580

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009207
Physical Location & Seq
Forward (+)
50870644 .. 50872042
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024580.1

Sequence Viewer

Length: 831 bp
ATGAAGAGAATAGGAGTAGTCGAGAAAATGCACGTGCTGGAGATCAACCTGATCTCCGGTCAGGGCTTGAAAGTCCCGTCCGGGAAGTTACGGCGGATGAAGACCTACGCAGTCGCCTGGGTCGACTCGAACCACAAGATCCGGACCCGGGTCGACAAAGTCGGAGCCGAGAACCCGACTTGGAACGACCGGTTCCTCTTCAAGGTCTCCTCCGAATTCCTCGCCAGGGAGACGTCCGGGATCACGATCGAGATCTACGCCGTCGGTGTCATCCGCGACCATCTCGTCGGCACCGTCCGATTGATCCTCAGCAACTTTCTTGACCTGGAATCCAAGATCCCGTCGTTCACGGCGCTGCAGATTCGCCGGCCTTCTGGAAGATTCCACGGCGTGTTGAACGTCGCCGCGTTGGTCAATCACGAGTCGGAGTGGTCCAAGGAGCTCGACGACGTCTCGGCGATCGGTTACCAAGATCTGATGGGCAAGGGAGAGAGCTTCCGGCGCCGGCGCCGGAGGGACAGCTTGACGAGGTCGACGGAGAATTTCTCCGGCAGCGAGTCGAGGGAGAATTCTTGCGCGGAGTCGGTGGAGAACTCCGACTGCGGCGACTCGTTCAAATCGGCGTCTCCGCCGTATAAATCGCCGTCTAAACCGACGTCTCCGGCGGCGGAGAAGAGAGCGCTGAAAGAGGCGAACGGCGTGAGGGATCTAGCGGCCGGAACCAGGGCTCTGAAGACAGCTGTCCCATCGGACGGCTCGCGATTCTTGTGCTGCTTGCTCACGACGCAGAGGAAGGTTCAGTCATCGTCGGATAAGAACGCAGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

276

Amino Acids

30.65

Weight (kDa)

9.69

Isoelectric Point (pI)

54.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 110 2e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AatII GACGTC 3 cut(s) 236, 453, 659
AccB1I GGYRCC 3 cut(s) 290, 501, 507
AccI GTMKAC 3 cut(s) 123, 153, 533
AccII CGCG 4 cut(s) 276, 407, 578, 760
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 9 cut(s) 94, 274, 405, 578, 603, 629, 665, 668, 713
AclWI GGATC 5 cut(s) 133, 248, 298, 331, 714
AcoI YGGCCR 1 cut(s) 714
AcsI RAATTY 3 cut(s) 215, 541, 568
AcuI CTGAAG 1 cut(s) 752
AcvI CACGTG 1 cut(s) 34
AcyI GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
AdeI CACNNNGTG 1 cut(s) 391
AfeI AGCGCT 1 cut(s) 681
AfiI CCNNNNNNNGG 5 cut(s) 148, 202, 226, 723, 752
AgeI ACCGGT 1 cut(s) 189
AgsI TTSAA 4 cut(s) 70, 202, 397, 616
AjnI CCWGG 4 cut(s) 116, 224, 324, 722
AluBI AGCT 4 cut(s) 442, 495, 522, 740
AluI AGCT 4 cut(s) 442, 495, 522, 740
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 5 cut(s) 211, 224, 457, 630, 663
AlwI GGATC 5 cut(s) 133, 248, 298, 331, 714
Ama87I CYCGRG 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 681
AoxI GGCC 2 cut(s) 368, 714
ApeKI GCWGC 3 cut(s) 355, 552, 771
ApoI RAATTY 3 cut(s) 215, 541, 568
AsiGI ACCGGT 1 cut(s) 189
AspLEI GCGC 5 cut(s) 355, 504, 510, 578, 682
AspS9I GGNCC 2 cut(s) 144, 432
AsuC2I CCSGG 4 cut(s) 82, 148, 149, 238
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 2 cut(s) 144, 432
BanI GGYRCC 3 cut(s) 290, 501, 507
BanII GRGCYC 2 cut(s) 444, 730
BauI CACGAG 1 cut(s) 419
BbrPI CACGTG 1 cut(s) 34
BbsI GAAGAC 2 cut(s) 107, 740
Bbv12I GWGCWC 1 cut(s) 444
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 3 cut(s) 342, 564, 758
BccI CCATC 3 cut(s) 288, 472, 754
BceAI ACGGC 8 cut(s) 107, 245, 366, 403, 616, 628, 712, 769
BcgI CGANNNNNNTGC 2 cut(s) 750, 784
BciT130I CCWGG 4 cut(s) 118, 226, 326, 724
BcnI CCSGG 4 cut(s) 82, 148, 149, 238
BcoDI GTCTC 5 cut(s) 211, 224, 457, 630, 663
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 356
BfoI RGCGCY 4 cut(s) 356, 505, 511, 683
BglII AGATCT 2 cut(s) 252, 472
BisI GCNGC 7 cut(s) 356, 405, 553, 604, 666, 714, 772
BlsI GCNGC 7 cut(s) 357, 406, 554, 605, 667, 715, 773
Bme1390I CCNGG 8 cut(s) 82, 118, 148, 149, 226, 238, 326, 724
Bme18I GGWCC 2 cut(s) 144, 432
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 2 cut(s) 144, 432
BmiI GGNNCC 7 cut(s) 146, 166, 194, 292, 503, 509, 721
BmrFI CCNGG 8 cut(s) 82, 118, 148, 149, 226, 238, 326, 724
BoxI GACNNNNGTC 2 cut(s) 149, 740
BpiI GAAGAC 2 cut(s) 107, 740
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 1 cut(s) 59
Bpu10I CCTNAGC 1 cut(s) 308
BpuMI CCSGG 4 cut(s) 82, 148, 149, 238
BsaAI YACGTR 1 cut(s) 34
BsaBI GATNNNNATC 2 cut(s) 245, 251
BsaHI GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
BsaI GGTCTC 1 cut(s) 211
BsaJI CCNNGG 6 cut(s) 117, 147, 225, 385, 435, 723
BsaWI WCCGGW 3 cut(s) 56, 141, 189
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 5 cut(s) 148, 202, 226, 723, 752
Bse118I RCCGGY 3 cut(s) 189, 366, 504
Bse8I GATNNNNATC 2 cut(s) 245, 251
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 4 cut(s) 118, 226, 326, 724
BseDI CCNNGG 6 cut(s) 117, 147, 225, 385, 435, 723
BseGI GGATG 2 cut(s) 102, 270
BseJI GATNNNNATC 2 cut(s) 245, 251
BseLI CCNNNNNNNGG 5 cut(s) 148, 202, 226, 723, 752
BseMII CTCAG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 714
BseXI GCAGC 3 cut(s) 342, 564, 758
Bsh1236I CGCG 4 cut(s) 276, 407, 578, 760
Bsh1285I CGRYCG 4 cut(s) 190, 249, 462, 717
BshFI GGCC 2 cut(s) 370, 716
BshNI GGYRCC 3 cut(s) 290, 501, 507
BshTI ACCGGT 1 cut(s) 189
BsiEI CGRYCG 4 cut(s) 190, 249, 462, 717
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 147
BslFI GGGAC 3 cut(s) 59, 530, 728
BslI CCNNNNNNNGG 5 cut(s) 148, 202, 226, 723, 752
BsmAI GTCTC 5 cut(s) 211, 224, 457, 630, 663
BsmBI CGTCTC 4 cut(s) 224, 457, 630, 663
BsmFI GGGAC 3 cut(s) 59, 530, 728
BsnI GGCC 2 cut(s) 370, 716
Bso31I GGTCTC 1 cut(s) 211
BsoBI CYCGRG 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 444, 730
Bsp13I TCCGGA 1 cut(s) 141
Bsp68I TCGCGA 1 cut(s) 760
BspACI CCGC 9 cut(s) 94, 274, 405, 578, 603, 629, 665, 668, 713
BspANI GGCC 2 cut(s) 370, 716
BspCNI CTCAG 1 cut(s) 321
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 4 cut(s) 276, 407, 578, 760
BspLI GGNNCC 7 cut(s) 146, 166, 194, 292, 503, 509, 721
BspMAI CTGCAG 1 cut(s) 360
BspPI GGATC 5 cut(s) 133, 248, 298, 331, 714
BspT107I GGYRCC 3 cut(s) 290, 501, 507
BspTNI GGTCTC 1 cut(s) 211
BsrFI RCCGGY 3 cut(s) 189, 366, 504
BssAI RCCGGY 3 cut(s) 189, 366, 504
BssECI CCNNGG 6 cut(s) 117, 147, 225, 385, 435, 723
BssNI GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 4 cut(s) 118, 226, 326, 724
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 2 cut(s) 203, 668
BstACI GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
BstBAI YACGTR 1 cut(s) 34
BstC8I GCNNGC 4 cut(s) 368, 506, 758, 776
BstDEI CTNAG 1 cut(s) 308
BstDSI CCRYGG 1 cut(s) 385
BstEII GGTNACC 1 cut(s) 464
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 2 cut(s) 102, 270
BstFNI CGCG 4 cut(s) 276, 407, 578, 760
BstH2I RGCGCY 4 cut(s) 356, 505, 511, 683
BstHHI GCGC 5 cut(s) 355, 504, 510, 578, 682
BstMAI GTCTC 5 cut(s) 211, 224, 457, 630, 663
BstMCI CGRYCG 4 cut(s) 190, 249, 462, 717
BstMWI GCNNNNNNNGC 2 cut(s) 501, 784
BstNI CCWGG 4 cut(s) 118, 226, 326, 724
BstPAI GACNNNNGTC 2 cut(s) 149, 740
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 8 cut(s) 80, 116, 146, 147, 224, 236, 324, 722
BstSFI CTRYAG 1 cut(s) 356
BstUI CGCG 4 cut(s) 276, 407, 578, 760
BstV1I GCAGC 3 cut(s) 342, 564, 758
BstV2I GAAGAC 2 cut(s) 107, 740
BstX2I RGATCY 5 cut(s) 138, 252, 336, 472, 706
BstYI RGATCY 5 cut(s) 138, 252, 336, 472, 706
BstZI CGGCCG 1 cut(s) 714
BsuRI GGCC 2 cut(s) 370, 716
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 2 cut(s) 102, 270
BtuMI TCGCGA 1 cut(s) 760
Cac8I GCNNGC 4 cut(s) 368, 506, 758, 776
CfoI GCGC 5 cut(s) 355, 504, 510, 578, 682
Cfr10I RCCGGY 3 cut(s) 189, 366, 504
Cfr13I GGNCC 2 cut(s) 144, 432
Cfr9I CCCGGG 1 cut(s) 147
CseI GACGC 2 cut(s) 612, 793
CspAI ACCGGT 1 cut(s) 189
DdeI CTNAG 1 cut(s) 308
DinI GGCGCC 2 cut(s) 503, 509
DraIII CACNNNGTG 1 cut(s) 391
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 714
EagI CGGCCG 1 cut(s) 714
Eam1104I CTCTTC 2 cut(s) 203, 668
EarI CTCTTC 2 cut(s) 203, 668
EciI GGCGGA 3 cut(s) 109, 618, 683
Ecl136II GAGCTC 1 cut(s) 442
EclXI CGGCCG 1 cut(s) 714
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 444, 730
Eco31I GGTCTC 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 144, 432
Eco47III AGCGCT 1 cut(s) 681
Eco52I CGGCCG 1 cut(s) 714
Eco53kI GAGCTC 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 752
Eco72I CACGTG 1 cut(s) 34
Eco88I CYCGRG 1 cut(s) 147
Eco91I GGTNACC 1 cut(s) 464
EcoICRI GAGCTC 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO65I GGTNACC 1 cut(s) 464
EcoRI GAATTC 2 cut(s) 215, 568
EcoRII CCWGG 4 cut(s) 116, 224, 324, 722
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 2 cut(s) 444, 730
EgeI GGCGCC 2 cut(s) 503, 509
EheI GGCGCC 2 cut(s) 503, 509
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 4 cut(s) 224, 457, 630, 663
FaiI YATR 1 cut(s) 636
FaqI GGGAC 3 cut(s) 59, 530, 728
FblI GTMKAC 3 cut(s) 123, 153, 533
Fnu4HI GCNGC 7 cut(s) 356, 405, 553, 604, 666, 714, 772
FokI GGATG 2 cut(s) 109, 257
FriOI GRGCYC 2 cut(s) 444, 730
Fsp4HI GCNGC 7 cut(s) 356, 405, 553, 604, 666, 714, 772
FspBI CTAG 1 cut(s) 710
GlaI GCGC 5 cut(s) 354, 503, 509, 577, 681
GluI GCNGC 7 cut(s) 356, 405, 553, 604, 666, 714, 772
GsuI CTGGAG 1 cut(s) 59
HaeII RGCGCY 4 cut(s) 356, 505, 511, 683
HaeIII GGCC 2 cut(s) 370, 716
HgaI GACGC 2 cut(s) 612, 793
HhaI GCGC 5 cut(s) 355, 504, 510, 578, 682
Hin1I GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
Hin6I GCGC 5 cut(s) 353, 502, 508, 576, 680
HinP1I GCGC 5 cut(s) 353, 502, 508, 576, 680
HincII GTYRAC 3 cut(s) 124, 154, 534
HindII GTYRAC 3 cut(s) 124, 154, 534
HinfI GANTC 9 cut(s) 125, 329, 361, 381, 422, 557, 581, 608, 762
Hpy166II GTNNAC 4 cut(s) 124, 154, 348, 534
Hpy188I TCNGA 9 cut(s) 164, 214, 299, 427, 477, 598, 732, 751, 811
Hpy188III TCNNGA 9 cut(s) 22, 142, 244, 250, 320, 375, 419, 759, 781
Hpy8I GTNNAC 4 cut(s) 124, 154, 348, 534
HpyAV CCTTC 2 cut(s) 381, 787
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 5 cut(s) 33, 233, 399, 450, 656
HpyCH4V TGCA 2 cut(s) 31, 358
HpyF10VI GCNNNNNNNGC 2 cut(s) 501, 784
HpyF3I CTNAG 1 cut(s) 308
HpySE526I ACGT 5 cut(s) 33, 233, 399, 450, 656
Hsp92I GRCGYC 6 cut(s) 233, 450, 502, 508, 623, 656
HspAI GCGC 5 cut(s) 353, 502, 508, 576, 680
KasI GGCGCC 2 cut(s) 501, 507
Kpn2I TCCGGA 1 cut(s) 141
KroI GCCGGC 2 cut(s) 366, 504
KroNI GCCGGC 2 cut(s) 368, 506
LmnI GCTCC 2 cut(s) 164, 439
Lsp1109I GCAGC 3 cut(s) 342, 564, 758
MaeI CTAG 1 cut(s) 710
MaeII ACGT 5 cut(s) 33, 233, 399, 450, 656
MaeIII GTNAC 2 cut(s) 87, 464
MboII GAAGA 6 cut(s) 16, 112, 190, 390, 685, 745
MflI RGATCY 5 cut(s) 138, 252, 336, 472, 706
MhlI GDGCHC 2 cut(s) 444, 730
MluCI AATT 3 cut(s) 215, 541, 568
Mly113I GGCGCC 2 cut(s) 502, 508
MlyI GAGTC 5 cut(s) 119, 431, 566, 590, 602
MmeI TCCRAC 4 cut(s) 142, 405, 621, 789
MreI CGCCGGCG 1 cut(s) 504
MroI TCCGGA 1 cut(s) 141
MroNI GCCGGC 2 cut(s) 366, 504
MspA1I CMGCKG 1 cut(s) 740
MspR9I CCNGG 8 cut(s) 82, 118, 148, 149, 226, 238, 326, 724
MvaI CCWGG 4 cut(s) 118, 226, 326, 724
MvnI CGCG 4 cut(s) 276, 407, 578, 760
MwoI GCNNNNNNNGC 2 cut(s) 501, 784
NaeI GCCGGC 2 cut(s) 368, 506
NarI GGCGCC 2 cut(s) 502, 508
NciI CCSGG 4 cut(s) 82, 148, 149, 238
NgoMIV GCCGGC 2 cut(s) 366, 504
NlaIV GGNNCC 7 cut(s) 146, 166, 194, 292, 503, 509, 721
NmeAIII GCCGAG 2 cut(s) 193, 434
NruI TCGCGA 1 cut(s) 760
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 291
PdiI GCCGGC 2 cut(s) 368, 506
PfeI GAWTC 4 cut(s) 329, 361, 381, 762
PflFI GACNNNGTC 3 cut(s) 158, 449, 529
PfoI TCCNGGA 2 cut(s) 80, 236
PinAI ACCGGT 1 cut(s) 189
PkrI GCNGC 7 cut(s) 357, 406, 554, 605, 667, 715, 773
Ple19I CGATCG 2 cut(s) 249, 462
PleI GAGTC 5 cut(s) 119, 430, 565, 589, 602
PluTI GGCGCC 2 cut(s) 505, 511
PmaCI CACGTG 1 cut(s) 34
PmlI CACGTG 1 cut(s) 34
PpsI GAGTC 5 cut(s) 119, 430, 565, 589, 602
Ppu21I YACGTR 1 cut(s) 34
PshAI GACNNNNGTC 2 cut(s) 149, 740
Psp124BI GAGCTC 1 cut(s) 444
Psp6I CCWGG 4 cut(s) 116, 224, 324, 722
PspCI CACGTG 1 cut(s) 34
PspEI GGTNACC 1 cut(s) 464
PspGI CCWGG 4 cut(s) 116, 224, 324, 722
PspN4I GGNNCC 7 cut(s) 146, 166, 194, 292, 503, 509, 721
PspPI GGNCC 2 cut(s) 144, 432
PstI CTGCAG 1 cut(s) 360
PsuI RGATCY 5 cut(s) 138, 252, 336, 472, 706
PsyI GACNNNGTC 3 cut(s) 158, 449, 529
PvuI CGATCG 2 cut(s) 249, 462
PvuII CAGCTG 1 cut(s) 740
RruI TCGCGA 1 cut(s) 760
SacI GAGCTC 1 cut(s) 444
SalI GTCGAC 3 cut(s) 122, 152, 532
SatI GCNGC 7 cut(s) 356, 405, 553, 604, 666, 714, 772
Sau96I GGNCC 2 cut(s) 144, 432
SchI GAGTC 5 cut(s) 119, 431, 566, 590, 602
ScrFI CCNGG 8 cut(s) 82, 118, 148, 149, 226, 238, 326, 724
SduI GDGCHC 2 cut(s) 444, 730
SfcI CTRYAG 1 cut(s) 356
SfoI GGCGCC 2 cut(s) 503, 509
SgrAI CRCCGGYG 1 cut(s) 504
SinI GGWCC 2 cut(s) 144, 432
SmaI CCCGGG 1 cut(s) 149
Sse9I AATT 3 cut(s) 215, 541, 568
SsiI CCGC 9 cut(s) 94, 274, 405, 578, 603, 629, 665, 668, 713
SspDI GGCGCC 2 cut(s) 501, 507
SspMI CTAG 1 cut(s) 710
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 8 cut(s) 80, 116, 146, 147, 224, 236, 324, 722
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 5 cut(s) 36, 236, 402, 453, 659
TaqI TCGA 8 cut(s) 21, 123, 128, 153, 249, 444, 533, 560
TasI AATT 3 cut(s) 215, 541, 568
TauI GCSGC 4 cut(s) 407, 606, 668, 716
TfiI GAWTC 4 cut(s) 329, 361, 381, 762
TseI GCWGC 3 cut(s) 355, 552, 771
TspDTI ATGAA 2 cut(s) 17, 113
TspGWI ACGGA 1 cut(s) 551
TspMI CCCGGG 1 cut(s) 147
Tth111I GACNNNGTC 3 cut(s) 158, 449, 529
VpaK11BI GGWCC 2 cut(s) 144, 432
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 3 cut(s) 215, 541, 568
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 3 cut(s) 123, 153, 533
XspI CTAG 1 cut(s) 710
ZraI GACGTC 3 cut(s) 234, 451, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.