Rorug01G0261900

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009207
Physical Location & Seq
Forward (+)
37205529 .. 37208577
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0261900.1

Sequence Viewer

Length: 1599 bp
ATGGTAAATCTCCTCCAAATGCTGGAACTGAAACTGAATGAAACCTTCTCAGTCCTGCTGCAACCCTTTTCCCTCAGATATTTACTACTTCTGGCCATTTTCCTCATTCTGTTTTACAGATGGTCTTCTTCTTATACCAAATCATCATCTGTAACACCATCTCCACCAAAGCTGCCTGTGATTGGAAATCTCCACCAACTAGGGTTGCTCCCTCACCACTCTCTTCAAACCCTTGCACAACGCTATGGGCCTGATCTAATGCTCCTTCATTTCGGAAGTAGGCCGGTCCTTATTGTCTCGTCTACCGAGGCTGTCTCCGAGATCTTCAAAAACCATGACCTCACCTTCTCTGATAAACCCAAGTATATCTTCGTAGAGAAGCTTATGTACAATTACAAGGACATCTTGACGGCACCTTATGGTGACTATTGGAGGCAGGTGAGGAGTTTATGCGTCTTAAATCTGTTGAGCAACAAAAGGGTCCGTTCGTTTCGTGCTGTGAGACAAGAGGAAACGAAACTCATGATGAGCAACATTGAACAGTCTTGTAGTACTGAAACATGCTCCTCCTCGTCATCATCATCGGTTTTGAATTTAAGCGAAATGTTTATGAAGCTTACTAATGATGTCATTTGTAGAGTCGCTATGGGTAGGAAGTACAGCGATCGGGAGGAAGGTCGTGGGAGGATGTTTATGGAGCTTTCTACAGAGCTGTCAGATTTGTTCACAAAGGTCAATATTGGTGACTATATCCCATGGCTTGCTTGGTTCACCCGTGTGAATGGTTTAGATGCAAAACTAGATGATCTGGCTAAGCGGATGGATGCCTTCATTGATATGGTTGTCCAAGAGCATATGGATAAATCAAACAATGGAAATGATGGTGATGATAACGAGGACAAAAAGGATTTGGTGGATGTTTTGCTTTCAGTTCAGAAAGAAAATGCACTTGGTTCTCCTATTGATCGAGTTAGCGTAAAGGCTATCATCCTGGATATTTTTGTGGGTGGCACTGACACCACATTTACCGCACTAGAGTGGGTAATGTCTGAACTTTTAAGAAATCCAGGGGTGATGAAGAAATTGCAAAATGAAGTTAGGGGCATAGTTGGAAATAAAAAGAACGTAACTGAGGAAGATTTGGTTGGAATGCCCTACTTGAAGGCAGTAACCAAAGAGAACTTTCGCTTACATCCTCCAGCTCCGTTACTATTGCCTAGGATGTGCAACAAAGATGTGAAAATTAAAGGTTACGACATTAAGGCTAATACACAAGTTATGATTAACGCATGGGCGCTTGGAAGAGATCCTAAATCATATACAAATCCGGAGCAGTTCAAGCCAGAAAGATTCTTGAATAGTACCATAGATTATAAAGGGAATAACTTCGAATTAATGCCATTTGGTACTGGCAGACGAGGCTGCCCTGGAATTCAGTTTGCTACGGTTCTTGAAGAGCTTGTTTTGGCAAATATTGTGCATAAGTTTGACTGGGCTTTGCCTCATGGTGCAAAAGAAGATGATTTAGACATGACTGAAACTGCAGGCACAACTGCCTGCAGAAAATATCCTCTTAAGGCAATTGCTACTCTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

532

Amino Acids

60.4

Weight (kDa)

7.58

Isoelectric Point (pI)

40.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 53 - 513 3.3e-105 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1374
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 22
AccI GTMKAC 1 cut(s) 302
AccIII TCCGGA 1 cut(s) 1327
AciI CCGC 2 cut(s) 817, 1029
AclWI GGATC 1 cut(s) 1301
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 2 cut(s) 592, 1431
AfaI GTAC 5 cut(s) 389, 553, 659, 1363, 1408
AfiI CCNNNNNNNGG 2 cut(s) 22, 182
AflII CTTAAG 1 cut(s) 1574
AgsI TTSAA 8 cut(s) 227, 328, 539, 592, 1162, 1339, 1357, 1454
AjnI CCWGG 3 cut(s) 990, 1066, 1426
AjuI GAANNNNNNNTTGG 6 cut(s) 353, 385, 933, 965, 1128, 1160
AleI CACNNNNGTG 2 cut(s) 776, 1036
AluBI AGCT 7 cut(s) 172, 382, 616, 700, 712, 1202, 1459
AluI AGCT 7 cut(s) 172, 382, 616, 700, 712, 1202, 1459
Alw26I GTCTC 3 cut(s) 301, 319, 496
AlwI GGATC 1 cut(s) 1301
Aor13HI TCCGGA 1 cut(s) 1327
AoxI GGCC 3 cut(s) 93, 248, 281
ApeKI GCWGC 3 cut(s) 58, 172, 1422
ApoI RAATTY 2 cut(s) 592, 1431
AseI ATTAAT 1 cut(s) 1394
Asp700I GAANNNNTTC 1 cut(s) 1385
AspA2I CCTAGG 1 cut(s) 1217
AspLEI GCGC 1 cut(s) 1297
AspS9I GGNCC 3 cut(s) 248, 286, 481
AsuHPI GGTGA 8 cut(s) 206, 334, 434, 451, 755, 763, 896, 1084
AsuII TTCGAA 1 cut(s) 1389
AvaII GGWCC 2 cut(s) 286, 481
AvrII CCTAGG 1 cut(s) 1217
BalI TGGCCA 1 cut(s) 95
BanI GGYRCC 1 cut(s) 412
BarI GAAGNNNNNNTAC 2 cut(s) 371, 403
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 45, 159, 1409
BccI CCATC 4 cut(s) 114, 166, 814, 875
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 3 cut(s) 992, 1068, 1428
BcoDI GTCTC 3 cut(s) 301, 319, 496
BfaI CTAG 4 cut(s) 200, 800, 1034, 1218
BfmI CTRYAG 3 cut(s) 705, 1542, 1558
BfoI RGCGCY 1 cut(s) 1298
BfrI CTTAAG 1 cut(s) 1574
BfuAI ACCTGC 1 cut(s) 427
BglII AGATCT 1 cut(s) 321
BisI GCNGC 3 cut(s) 59, 173, 1423
BlnI CCTAGG 1 cut(s) 1217
BlpI GCTNAGC 1 cut(s) 813
BlsI GCNGC 3 cut(s) 60, 174, 1424
BmcAI AGTACT 1 cut(s) 553
Bme1390I CCNGG 3 cut(s) 992, 1068, 1428
Bme18I GGWCC 2 cut(s) 286, 481
BmgT120I GGNCC 3 cut(s) 248, 286, 481
BmiI GGNNCC 2 cut(s) 414, 482
BmrFI CCNGG 3 cut(s) 992, 1068, 1428
BmrI ACTGGG 1 cut(s) 1501
BmsI GCATC 2 cut(s) 781, 814
BmuI ACTGGG 1 cut(s) 1501
BpiI GAAGAC 1 cut(s) 117
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 1182
Bpu1102I GCTNAGC 1 cut(s) 813
Bpu14I TTCGAA 1 cut(s) 1389
BsaJI CCNNGG 5 cut(s) 306, 755, 1067, 1217, 1426
BsaWI WCCGGW 1 cut(s) 1327
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 182
Bse118I RCCGGY 1 cut(s) 283
Bse1I ACTGG 2 cut(s) 1414, 1496
BseAI TCCGGA 1 cut(s) 1327
BseBI CCWGG 3 cut(s) 992, 1068, 1428
BseDI CCNNGG 5 cut(s) 306, 755, 1067, 1217, 1426
BseGI GGATG 7 cut(s) 693, 825, 829, 922, 987, 1192, 1227
BseLI CCNNNNNNNGG 2 cut(s) 22, 182
BseMII CTCAG 3 cut(s) 63, 88, 1122
BseNI ACTGG 2 cut(s) 1414, 1496
BseRI GAGGAG 3 cut(s) 457, 556, 559
BseXI GCAGC 3 cut(s) 45, 159, 1409
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 3 cut(s) 95, 250, 283
BshNI GGYRCC 1 cut(s) 412
BsiEI CGRYCG 1 cut(s) 667
BsiSI CCGG 2 cut(s) 284, 1328
BslI CCNNNNNNNGG 2 cut(s) 22, 182
BsmAI GTCTC 3 cut(s) 301, 319, 496
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 3 cut(s) 95, 250, 283
Bsp119I TTCGAA 1 cut(s) 1389
Bsp13I TCCGGA 1 cut(s) 1327
Bsp1407I TGTACA 1 cut(s) 387
Bsp143I GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
Bsp1720I GCTNAGC 1 cut(s) 813
Bsp19I CCATGG 1 cut(s) 755
BspACI CCGC 2 cut(s) 817, 1029
BspANI GGCC 3 cut(s) 95, 250, 283
BspCNI CTCAG 3 cut(s) 62, 87, 1123
BspEI TCCGGA 1 cut(s) 1327
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 2 cut(s) 414, 482
BspMAI CTGCAG 2 cut(s) 1546, 1562
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 1 cut(s) 1301
BspQI GCTCTTC 1 cut(s) 1449
BspT104I TTCGAA 1 cut(s) 1389
BspT107I GGYRCC 1 cut(s) 412
BspTI CTTAAG 1 cut(s) 1574
BsrFI RCCGGY 1 cut(s) 283
BsrGI TGTACA 1 cut(s) 387
BsrI ACTGG 2 cut(s) 1414, 1496
BssAI RCCGGY 1 cut(s) 283
BssECI CCNNGG 5 cut(s) 306, 755, 1067, 1217, 1426
BssMI GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
BssT1I CCWWGG 2 cut(s) 755, 1217
Bst2UI CCWGG 3 cut(s) 992, 1068, 1428
Bst4CI ACNGT 2 cut(s) 543, 1447
Bst6I CTCTTC 3 cut(s) 228, 1297, 1449
BstAFI CTTAAG 1 cut(s) 1574
BstAUI TGTACA 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 1389
BstC8I GCNNGC 3 cut(s) 762, 1546, 1558
BstDEI CTNAG 4 cut(s) 49, 74, 813, 1131
BstDSI CCRYGG 1 cut(s) 755
BstF5I GGATG 7 cut(s) 693, 825, 829, 922, 987, 1192, 1227
BstH2I RGCGCY 1 cut(s) 1298
BstHHI GCGC 1 cut(s) 1297
BstKTI GATC 6 cut(s) 256, 324, 667, 808, 967, 1309
BstMAI GTCTC 3 cut(s) 301, 319, 496
BstMBI GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 2 cut(s) 1339, 1419
BstNI CCWGG 3 cut(s) 992, 1068, 1428
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 990, 1066, 1426
BstSFI CTRYAG 3 cut(s) 705, 1542, 1558
BstV1I GCAGC 3 cut(s) 45, 159, 1409
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 2 cut(s) 321, 1306
BstYI RGATCY 2 cut(s) 321, 1306
BsuRI GGCC 3 cut(s) 95, 250, 283
BtgI CCRYGG 1 cut(s) 755
BtsCI GGATG 7 cut(s) 693, 825, 829, 922, 987, 1192, 1227
BtsIMutI CAGTG 1 cut(s) 1011
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 3 cut(s) 762, 1546, 1558
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 1297
Cfr10I RCCGGY 1 cut(s) 283
Cfr13I GGNCC 3 cut(s) 248, 286, 481
CseI GACGC 1 cut(s) 442
Csp6I GTAC 5 cut(s) 388, 552, 658, 1362, 1407
CviAII CATG 7 cut(s) 335, 523, 561, 756, 1290, 1505, 1531
CviQI GTAC 5 cut(s) 388, 552, 658, 1362, 1407
DdeI CTNAG 4 cut(s) 49, 74, 813, 1131
DpnI GATC 6 cut(s) 255, 323, 666, 807, 966, 1308
DpnII GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 3 cut(s) 228, 1297, 1449
EarI CTCTTC 3 cut(s) 228, 1297, 1449
Eco130I CCWWGG 2 cut(s) 755, 1217
Eco47I GGWCC 2 cut(s) 286, 481
EcoRI GAATTC 1 cut(s) 1431
EcoRII CCWGG 3 cut(s) 990, 1066, 1426
EcoT14I CCWWGG 2 cut(s) 755, 1217
ErhI CCWWGG 2 cut(s) 755, 1217
FaeI CATG 7 cut(s) 338, 526, 564, 759, 1293, 1508, 1534
FalI AAGNNNNNCTT 4 cut(s) 353, 385, 389, 421
FatI CATG 7 cut(s) 334, 522, 560, 755, 1289, 1504, 1530
FauNDI CATATG 1 cut(s) 855
FblI GTMKAC 1 cut(s) 302
Fnu4HI GCNGC 3 cut(s) 59, 173, 1423
FokI GGATG 7 cut(s) 700, 832, 836, 929, 974, 1179, 1234
Fsp4HI GCNGC 3 cut(s) 59, 173, 1423
FspBI CTAG 4 cut(s) 200, 800, 1034, 1218
GlaI GCGC 1 cut(s) 1296
GluI GCNGC 3 cut(s) 59, 173, 1423
GsuI CTGGAG 1 cut(s) 1182
HaeII RGCGCY 1 cut(s) 1298
HaeIII GGCC 3 cut(s) 95, 250, 283
HapII CCGG 2 cut(s) 284, 1328
HgaI GACGC 1 cut(s) 442
HhaI GCGC 1 cut(s) 1297
Hin1II CATG 7 cut(s) 338, 526, 564, 759, 1293, 1508, 1534
Hin6I GCGC 1 cut(s) 1295
HinP1I GCGC 1 cut(s) 1295
HindIII AAGCTT 2 cut(s) 380, 614
HinfI GANTC 2 cut(s) 639, 1350
HpaII CCGG 2 cut(s) 284, 1328
HphI GGTGA 8 cut(s) 206, 334, 434, 451, 755, 763, 896, 1084
Hpy166II GTNNAC 3 cut(s) 303, 726, 771
Hpy188I TCNGA 7 cut(s) 77, 275, 319, 352, 718, 936, 1051
Hpy188III TCNNGA 6 cut(s) 406, 523, 668, 1328, 1354, 1451
Hpy8I GTNNAC 3 cut(s) 303, 726, 771
HpyAV CCTTC 6 cut(s) 55, 275, 355, 668, 838, 1156
HpyCH4III ACNGT 2 cut(s) 543, 1447
HpyCH4IV ACGT 1 cut(s) 1125
HpyF10VI GCNNNNNNNGC 2 cut(s) 1339, 1419
HpyF3I CTNAG 4 cut(s) 49, 74, 813, 1131
HpySE526I ACGT 1 cut(s) 1125
Hsp92II CATG 7 cut(s) 338, 526, 564, 759, 1293, 1508, 1534
HspAI GCGC 1 cut(s) 1295
Kpn2I TCCGGA 1 cut(s) 1327
Kzo9I GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
LguI GCTCTTC 1 cut(s) 1449
LmnI GCTCC 6 cut(s) 213, 267, 569, 697, 1207, 1330
Lsp1109I GCAGC 3 cut(s) 45, 159, 1409
LweI GCATC 2 cut(s) 781, 814
MaeI CTAG 4 cut(s) 200, 800, 1034, 1218
MaeII ACGT 1 cut(s) 1125
MaeIII GTNAC 7 cut(s) 151, 422, 743, 1126, 1168, 1206, 1250
MalI GATC 6 cut(s) 255, 323, 666, 807, 966, 1308
MboI GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
MfeI CAATTG 1 cut(s) 1581
MflI RGATCY 2 cut(s) 321, 1306
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 7 cut(s) 391, 592, 1082, 1242, 1391, 1431, 1581
MluNI TGGCCA 1 cut(s) 95
MlyI GAGTC 1 cut(s) 648
MmeI TCCRAC 2 cut(s) 1090, 1126
Mox20I TGGCCA 1 cut(s) 95
MroI TCCGGA 1 cut(s) 1327
MroXI GAANNNNTTC 1 cut(s) 1385
MscI TGGCCA 1 cut(s) 95
MseI TTAA 8 cut(s) 458, 596, 1058, 1245, 1260, 1284, 1394, 1575
MslI CAYNNNNRTG 3 cut(s) 776, 836, 1036
Msp20I TGGCCA 1 cut(s) 95
MspCI CTTAAG 1 cut(s) 1574
MspI CCGG 2 cut(s) 284, 1328
MspR9I CCNGG 3 cut(s) 992, 1068, 1428
MunI CAATTG 1 cut(s) 1581
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 3 cut(s) 992, 1068, 1428
MwoI GCNNNNNNNGC 2 cut(s) 1339, 1419
NcoI CCATGG 1 cut(s) 755
NdeI CATATG 1 cut(s) 855
NdeII GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
NlaIII CATG 7 cut(s) 338, 526, 564, 759, 1293, 1508, 1534
NlaIV GGNNCC 2 cut(s) 414, 482
NmuCI GTSAC 2 cut(s) 422, 743
NspI RCATGY 1 cut(s) 564
NspV TTCGAA 1 cut(s) 1389
OliI CACNNNNGTG 2 cut(s) 776, 1036
PagI TCATGA 1 cut(s) 522
PaqCI CACCTGC 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 1449
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 1385
PfeI GAWTC 1 cut(s) 1350
PflMI CCANNNNNTGG 1 cut(s) 22
PfoI TCCNGGA 1 cut(s) 990
PkrI GCNGC 3 cut(s) 60, 174, 1424
Ple19I CGATCG 1 cut(s) 667
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
PshBI ATTAAT 1 cut(s) 1394
PsiI TTATAA 1 cut(s) 1374
Psp6I CCWGG 3 cut(s) 990, 1066, 1426
PspGI CCWGG 3 cut(s) 990, 1066, 1426
PspN4I GGNNCC 2 cut(s) 414, 482
PspPI GGNCC 3 cut(s) 248, 286, 481
PstI CTGCAG 2 cut(s) 1546, 1562
PsuI RGATCY 2 cut(s) 321, 1306
PvuI CGATCG 1 cut(s) 667
RsaI GTAC 5 cut(s) 389, 553, 659, 1363, 1408
RsaNI GTAC 5 cut(s) 388, 552, 658, 1362, 1407
RseI CAYNNNNRTG 3 cut(s) 776, 836, 1036
SapI GCTCTTC 1 cut(s) 1449
SaqAI TTAA 8 cut(s) 458, 596, 1058, 1245, 1260, 1284, 1394, 1575
SatI GCNGC 3 cut(s) 59, 173, 1423
Sau3AI GATC 6 cut(s) 253, 321, 664, 805, 964, 1306
Sau96I GGNCC 3 cut(s) 248, 286, 481
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 1 cut(s) 648
ScrFI CCNGG 3 cut(s) 992, 1068, 1428
SfaNI GCATC 2 cut(s) 781, 814
SfcI CTRYAG 3 cut(s) 705, 1542, 1558
SfuI TTCGAA 1 cut(s) 1389
SinI GGWCC 2 cut(s) 286, 481
SmiMI CAYNNNNRTG 3 cut(s) 776, 836, 1036
SmlI CTYRAG 1 cut(s) 1574
SmoI CTYRAG 1 cut(s) 1574
Sse9I AATT 7 cut(s) 391, 592, 1082, 1242, 1391, 1431, 1581
SsiI CCGC 2 cut(s) 817, 1029
SspI AATATT 2 cut(s) 739, 1474
SspMI CTAG 4 cut(s) 200, 800, 1034, 1218
StyD4I CCNGG 3 cut(s) 990, 1066, 1426
StyI CCWWGG 2 cut(s) 755, 1217
TaaI ACNGT 2 cut(s) 543, 1447
TaiI ACGT 1 cut(s) 1128
TaqI TCGA 2 cut(s) 967, 1389
TasI AATT 7 cut(s) 391, 592, 1082, 1242, 1391, 1431, 1581
TatI WGTACW 3 cut(s) 387, 551, 657
TfiI GAWTC 1 cut(s) 1350
Tru1I TTAA 8 cut(s) 458, 596, 1058, 1245, 1260, 1284, 1394, 1575
Tru9I TTAA 8 cut(s) 458, 596, 1058, 1245, 1260, 1284, 1394, 1575
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 2 cut(s) 422, 743
TseI GCWGC 3 cut(s) 58, 172, 1422
Tsp45I GTSAC 2 cut(s) 422, 743
TspDTI ATGAA 6 cut(s) 54, 257, 626, 820, 1091, 1107
TspGWI ACGGA 2 cut(s) 473, 1194
TspRI CASTG 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 1574
VpaK11BI GGWCC 2 cut(s) 286, 481
VspI ATTAAT 1 cut(s) 1394
XapI RAATTY 2 cut(s) 592, 1431
XceI RCATGY 1 cut(s) 564
XcmI CCANNNNNNNNNTGG 1 cut(s) 762
XmaJI CCTAGG 1 cut(s) 1217
XmiI GTMKAC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 1385
XspI CTAG 4 cut(s) 200, 800, 1034, 1218
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.