FvH4_7g15700

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009207
Physical Location & Seq
Forward (+)
13540898 .. 13542872
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15700.t1

Sequence Viewer

Length: 882 bp
ATGAAGAAAGTAGGCGCAGTGGAGAAGATGCAAGTCCTGGAGATCAACCTGATCTCCGGCCAGGGCTTGAAGCCTCCGTCGGGGACGCTACGGCGGATGAAGACCTACGCCGTCGCCTGGGTCGAGTCGAGCCACAAGATCCGGACCCGGGTCGACAAAGTCGGAGCCGAAAACCCGACTTGGAACGACCGCTTCCTCTTCAAAGTCTCCTCCGAATTCCTCGCCAGAGAAACCTCCGGGATCACGATCGAGATCTACGCCGTCGGATCCTTCCGCGACCATCTCGTCGGCACCGTCCGATTGATCCTCAGCAACTTTCTTGACCTGGATTCCAAGATCCCGTCCTTCACGGCGCTCCAGATCCGCCGGAGGTCCGGTAGGTTCCACGGCGTTCTCAACGTCGCCGCGCTGGTGAATCACGAGTCGGAGTGGTCCAAGGAGCTAGACGACGTCTCGGCGATCGGGTACCAAGATTTGATGGGGAAAGGCGAGAGCTTCCGGCGCCGGCGACGGAGGGACAGCCTGACTCGGTCGACGGCAAGCTTCTCCGGCAGCGAGTCGAGGGAGAATTCCTCGTGCAACGAGTCGGTTAACTCGGACAGCGGCGACTCTTTACCTTCGTCTCCGGCGTACAAATCGCCTTCGAAGCTGACGTCACCGGCGTACAAATCGCCTTCGAAGCTGAGTTCTCCGGCGTACAAATCGCCGTCGAAAGCGACATCGCCGGCGGCGGAGAAGAGGGCGCTGAAGGAGATGAACGGCGTGAGGGATCTAGCGGCCGGAACCAAGGCTCTGAGGACAGCTGTCCCATCGGACGGCGCCCGCTTCTTGTGCTGCTTGCTCACGACGCAGAGGAAGGTTCAGTCATCGGAGGATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

294

Amino Acids

32.25

Weight (kDa)

9.91

Isoelectric Point (pI)

64.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 12 - 109 1.1e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AatII GACGTC 2 cut(s) 453, 656
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 4 cut(s) 290, 465, 501, 818
AccI GTMKAC 2 cut(s) 153, 533
AccII CGCG 2 cut(s) 276, 407
AccIII TCCGGA 1 cut(s) 141
AclWI GGATC 8 cut(s) 133, 248, 261, 274, 298, 331, 355, 777
AcoI YGGCCR 2 cut(s) 58, 777
AcsI RAATTY 2 cut(s) 215, 568
AcuI CTGAAG 1 cut(s) 767
AcyI GRCGYC 4 cut(s) 450, 502, 653, 819
AfaI GTAC 4 cut(s) 467, 632, 665, 698
AfiI CCNNNNNNNGG 4 cut(s) 80, 117, 148, 815
AgsI TTSAA 2 cut(s) 70, 202
AjnI CCWGG 4 cut(s) 36, 60, 116, 324
AluBI AGCT 6 cut(s) 442, 495, 543, 649, 682, 803
AluI AGCT 6 cut(s) 442, 495, 543, 649, 682, 803
Alw26I GTCTC 3 cut(s) 211, 457, 627
AlwI GGATC 8 cut(s) 133, 248, 261, 274, 298, 331, 355, 777
Ama87I CYCGRG 1 cut(s) 147
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 2 cut(s) 58, 777
ApeKI GCWGC 2 cut(s) 552, 834
ApoI RAATTY 2 cut(s) 215, 568
ArsI GACNNNNNNTTYG 2 cut(s) 638, 670
Asp718I GGTACC 1 cut(s) 465
AspLEI GCGC 6 cut(s) 17, 355, 409, 504, 745, 821
AspS9I GGNCC 3 cut(s) 144, 372, 432
AsuC2I CCSGG 3 cut(s) 148, 149, 238
AsuHPI GGTGA 2 cut(s) 424, 648
AsuII TTCGAA 2 cut(s) 644, 677
AvaI CYCGRG 1 cut(s) 147
AvaII GGWCC 3 cut(s) 144, 372, 432
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 4 cut(s) 290, 465, 501, 818
BauI CACGAG 2 cut(s) 419, 574
BbsI GAAGAC 1 cut(s) 107
BbvCI CCTCAGC 1 cut(s) 308
BbvI GCAGC 2 cut(s) 564, 821
BccI CCATC 3 cut(s) 288, 472, 817
BceAI ACGGC 9 cut(s) 95, 107, 245, 366, 403, 552, 691, 775, 832
BciT130I CCWGG 4 cut(s) 38, 62, 118, 326
BcnI CCSGG 3 cut(s) 148, 149, 238
BcoDI GTCTC 3 cut(s) 211, 457, 627
BfaI CTAG 2 cut(s) 443, 773
BfoI RGCGCY 4 cut(s) 356, 505, 746, 822
BglII AGATCT 1 cut(s) 252
BisI GCNGC 6 cut(s) 405, 553, 604, 729, 777, 835
BlsI GCNGC 6 cut(s) 406, 554, 605, 730, 778, 836
Bme1390I CCNGG 7 cut(s) 38, 62, 118, 148, 149, 238, 326
Bme18I GGWCC 3 cut(s) 144, 372, 432
BmeT110I CYCGRG 1 cut(s) 147
BmgT120I GGNCC 3 cut(s) 144, 372, 432
BmiI GGNNCC 9 cut(s) 146, 166, 268, 292, 383, 467, 503, 784, 820
BmrFI CCNGG 7 cut(s) 38, 62, 118, 148, 149, 238, 326
BmsI GCATC 1 cut(s) 18
BoxI GACNNNNGTC 2 cut(s) 149, 803
BpiI GAAGAC 1 cut(s) 107
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 2 cut(s) 59, 341
Bpu10I CCTNAGC 1 cut(s) 308
Bpu14I TTCGAA 2 cut(s) 644, 677
BpuMI CCSGG 3 cut(s) 148, 149, 238
BsaBI GATNNNNATC 2 cut(s) 245, 251
BsaHI GRCGYC 4 cut(s) 450, 502, 653, 819
BsaJI CCNNGG 6 cut(s) 61, 117, 147, 385, 435, 786
BsaWI WCCGGW 2 cut(s) 141, 374
BsaXI ACNNNNNCTCC 5 cut(s) 38, 68, 361, 391, 863
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 117, 148, 815
Bse118I RCCGGY 3 cut(s) 504, 658, 724
Bse8I GATNNNNATC 2 cut(s) 245, 251
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 4 cut(s) 38, 62, 118, 326
BseDI CCNNGG 6 cut(s) 61, 117, 147, 385, 435, 786
BseGI GGATG 1 cut(s) 102
BseJI GATNNNNATC 2 cut(s) 245, 251
BseLI CCNNNNNNNGG 4 cut(s) 80, 117, 148, 815
BseMII CTCAG 3 cut(s) 322, 674, 785
BseRI GAGGAG 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 777
BseXI GCAGC 2 cut(s) 564, 821
Bsh1236I CGCG 2 cut(s) 276, 407
Bsh1285I CGRYCG 5 cut(s) 190, 249, 462, 533, 780
BshFI GGCC 2 cut(s) 60, 779
BshNI GGYRCC 4 cut(s) 290, 465, 501, 818
BsiEI CGRYCG 5 cut(s) 190, 249, 462, 533, 780
BsiHKCI CYCGRG 1 cut(s) 147
BslFI GGGAC 3 cut(s) 97, 530, 791
BslI CCNNNNNNNGG 4 cut(s) 80, 117, 148, 815
BsmAI GTCTC 3 cut(s) 211, 457, 627
BsmBI CGTCTC 2 cut(s) 457, 627
BsmFI GGGAC 3 cut(s) 97, 530, 791
BsnI GGCC 2 cut(s) 60, 779
BsoBI CYCGRG 1 cut(s) 147
Bsp119I TTCGAA 2 cut(s) 644, 677
Bsp13I TCCGGA 1 cut(s) 141
BspANI GGCC 2 cut(s) 60, 779
BspCNI CTCAG 3 cut(s) 321, 675, 786
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 2 cut(s) 276, 407
BspLI GGNNCC 9 cut(s) 146, 166, 268, 292, 383, 467, 503, 784, 820
BspPI GGATC 8 cut(s) 133, 248, 261, 274, 298, 331, 355, 777
BspT104I TTCGAA 2 cut(s) 644, 677
BspT107I GGYRCC 4 cut(s) 290, 465, 501, 818
BsrFI RCCGGY 3 cut(s) 504, 658, 724
BssAI RCCGGY 3 cut(s) 504, 658, 724
BssECI CCNNGG 6 cut(s) 61, 117, 147, 385, 435, 786
BssNI GRCGYC 4 cut(s) 450, 502, 653, 819
BssSI CACGAG 2 cut(s) 419, 574
BssT1I CCWWGG 2 cut(s) 435, 786
Bst2BI CACGAG 2 cut(s) 419, 574
Bst2UI CCWGG 4 cut(s) 38, 62, 118, 326
Bst4CI ACNGT 1 cut(s) 295
Bst6I CTCTTC 2 cut(s) 203, 731
BstACI GRCGYC 4 cut(s) 450, 502, 653, 819
BstBI TTCGAA 2 cut(s) 644, 677
BstC8I GCNNGC 5 cut(s) 506, 541, 726, 823, 839
BstDEI CTNAG 3 cut(s) 308, 683, 794
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 1 cut(s) 102
BstFNI CGCG 2 cut(s) 276, 407
BstH2I RGCGCY 4 cut(s) 356, 505, 746, 822
BstHHI GCGC 6 cut(s) 17, 355, 409, 504, 745, 821
BstMAI GTCTC 3 cut(s) 211, 457, 627
BstMCI CGRYCG 5 cut(s) 190, 249, 462, 533, 780
BstMWI GCNNNNNNNGC 6 cut(s) 501, 549, 646, 679, 831, 847
BstNI CCWGG 4 cut(s) 38, 62, 118, 326
BstPAI GACNNNNGTC 2 cut(s) 149, 803
BstSCI CCNGG 7 cut(s) 36, 60, 116, 146, 147, 236, 324
BstUI CGCG 2 cut(s) 276, 407
BstV1I GCAGC 2 cut(s) 564, 821
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 6 cut(s) 138, 252, 266, 336, 360, 769
BstYI RGATCY 6 cut(s) 138, 252, 266, 336, 360, 769
BstZI CGGCCG 1 cut(s) 777
BsuRI GGCC 2 cut(s) 60, 779
BtgI CCRYGG 1 cut(s) 385
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 1 cut(s) 102
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 5 cut(s) 506, 541, 726, 823, 839
CfoI GCGC 6 cut(s) 17, 355, 409, 504, 745, 821
Cfr10I RCCGGY 3 cut(s) 504, 658, 724
Cfr13I GGNCC 3 cut(s) 144, 372, 432
Cfr9I CCCGGG 1 cut(s) 147
CseI GACGC 2 cut(s) 94, 856
Csp6I GTAC 4 cut(s) 466, 631, 664, 697
CviQI GTAC 4 cut(s) 466, 631, 664, 697
DdeI CTNAG 3 cut(s) 308, 683, 794
DinI GGCGCC 2 cut(s) 503, 820
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 2 cut(s) 58, 777
EagI CGGCCG 1 cut(s) 777
Eam1104I CTCTTC 2 cut(s) 203, 731
EarI CTCTTC 2 cut(s) 203, 731
EciI GGCGGA 3 cut(s) 109, 353, 746
EclXI CGGCCG 1 cut(s) 777
Eco130I CCWWGG 2 cut(s) 435, 786
Eco47I GGWCC 3 cut(s) 144, 372, 432
Eco52I CGGCCG 1 cut(s) 777
Eco57I CTGAAG 1 cut(s) 767
Eco88I CYCGRG 1 cut(s) 147
EcoRI GAATTC 2 cut(s) 215, 568
EcoRII CCWGG 4 cut(s) 36, 60, 116, 324
EcoT14I CCWWGG 2 cut(s) 435, 786
EgeI GGCGCC 2 cut(s) 503, 820
EheI GGCGCC 2 cut(s) 503, 820
ErhI CCWWGG 2 cut(s) 435, 786
Esp3I CGTCTC 2 cut(s) 457, 627
FaqI GGGAC 3 cut(s) 97, 530, 791
FauI CCCGC 1 cut(s) 830
FblI GTMKAC 2 cut(s) 153, 533
Fnu4HI GCNGC 6 cut(s) 405, 553, 604, 729, 777, 835
FokI GGATG 1 cut(s) 109
Fsp4HI GCNGC 6 cut(s) 405, 553, 604, 729, 777, 835
FspBI CTAG 2 cut(s) 443, 773
GlaI GCGC 6 cut(s) 16, 354, 408, 503, 744, 820
GluI GCNGC 6 cut(s) 405, 553, 604, 729, 777, 835
GsuI CTGGAG 2 cut(s) 59, 341
HaeII RGCGCY 4 cut(s) 356, 505, 746, 822
HaeIII GGCC 2 cut(s) 60, 779
HgaI GACGC 2 cut(s) 94, 856
HhaI GCGC 6 cut(s) 17, 355, 409, 504, 745, 821
Hin1I GRCGYC 4 cut(s) 450, 502, 653, 819
Hin6I GCGC 6 cut(s) 15, 353, 407, 502, 743, 819
HinP1I GCGC 6 cut(s) 15, 353, 407, 502, 743, 819
HincII GTYRAC 3 cut(s) 154, 534, 592
HindII GTYRAC 3 cut(s) 154, 534, 592
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 8 cut(s) 125, 329, 415, 422, 526, 557, 584, 608
HpaI GTTAAC 1 cut(s) 592
HphI GGTGA 2 cut(s) 424, 648
Hpy166II GTNNAC 3 cut(s) 154, 534, 592
Hpy188I TCNGA 9 cut(s) 164, 214, 266, 299, 427, 598, 795, 814, 871
Hpy188III TCNNGA 7 cut(s) 142, 244, 250, 320, 358, 419, 844
Hpy8I GTNNAC 3 cut(s) 154, 534, 592
HpyAV CCTTC 7 cut(s) 280, 355, 627, 651, 684, 742, 850
HpyCH4III ACNGT 1 cut(s) 295
HpyCH4IV ACGT 3 cut(s) 399, 450, 653
HpyCH4V TGCA 2 cut(s) 31, 579
HpyF10VI GCNNNNNNNGC 6 cut(s) 501, 549, 646, 679, 831, 847
HpyF3I CTNAG 3 cut(s) 308, 683, 794
HpySE526I ACGT 3 cut(s) 399, 450, 653
Hsp92I GRCGYC 4 cut(s) 450, 502, 653, 819
HspAI GCGC 6 cut(s) 15, 353, 407, 502, 743, 819
KasI GGCGCC 2 cut(s) 501, 818
Kpn2I TCCGGA 1 cut(s) 141
KpnI GGTACC 1 cut(s) 469
KroI GCCGGC 2 cut(s) 504, 724
KroNI GCCGGC 2 cut(s) 506, 726
KspAI GTTAAC 1 cut(s) 592
LmnI GCTCC 3 cut(s) 164, 360, 439
Lsp1109I GCAGC 2 cut(s) 564, 821
LweI GCATC 1 cut(s) 18
MaeI CTAG 2 cut(s) 443, 773
MaeII ACGT 3 cut(s) 399, 450, 653
MaeIII GTNAC 1 cut(s) 654
MboII GAAGA 5 cut(s) 16, 37, 112, 190, 748
MflI RGATCY 6 cut(s) 138, 252, 266, 336, 360, 769
MluCI AATT 2 cut(s) 215, 568
Mly113I GGCGCC 2 cut(s) 502, 819
MlyI GAGTC 6 cut(s) 134, 431, 520, 566, 593, 602
MmeI TCCRAC 3 cut(s) 142, 244, 405
MreI CGCCGGCG 2 cut(s) 504, 724
MroI TCCGGA 1 cut(s) 141
MroNI GCCGGC 2 cut(s) 504, 724
MseI TTAA 1 cut(s) 591
MspA1I CMGCKG 2 cut(s) 603, 803
MspR9I CCNGG 7 cut(s) 38, 62, 118, 148, 149, 238, 326
MvaI CCWGG 4 cut(s) 38, 62, 118, 326
MvnI CGCG 2 cut(s) 276, 407
MwoI GCNNNNNNNGC 6 cut(s) 501, 549, 646, 679, 831, 847
NaeI GCCGGC 2 cut(s) 506, 726
NarI GGCGCC 2 cut(s) 502, 819
NciI CCSGG 3 cut(s) 148, 149, 238
NgoMIV GCCGGC 2 cut(s) 504, 724
NlaIV GGNNCC 9 cut(s) 146, 166, 268, 292, 383, 467, 503, 784, 820
NmeAIII GCCGAG 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 654
NspV TTCGAA 2 cut(s) 644, 677
PcsI WCGNNNNNNNCGW 5 cut(s) 120, 291, 626, 650, 659
PdiI GCCGGC 2 cut(s) 506, 726
PfeI GAWTC 2 cut(s) 329, 415
PflFI GACNNNGTC 3 cut(s) 158, 449, 529
PfoI TCCNGGA 2 cut(s) 36, 236
PkrI GCNGC 6 cut(s) 406, 554, 605, 730, 778, 836
Ple19I CGATCG 2 cut(s) 249, 462
PleI GAGTC 6 cut(s) 133, 430, 520, 565, 592, 602
PluTI GGCGCC 2 cut(s) 505, 822
PpsI GAGTC 6 cut(s) 133, 430, 520, 565, 592, 602
PshAI GACNNNNGTC 2 cut(s) 149, 803
Psp6I CCWGG 4 cut(s) 36, 60, 116, 324
PspGI CCWGG 4 cut(s) 36, 60, 116, 324
PspN4I GGNNCC 9 cut(s) 146, 166, 268, 292, 383, 467, 503, 784, 820
PspPI GGNCC 3 cut(s) 144, 372, 432
PsuI RGATCY 6 cut(s) 138, 252, 266, 336, 360, 769
PsyI GACNNNGTC 3 cut(s) 158, 449, 529
PvuI CGATCG 2 cut(s) 249, 462
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 4 cut(s) 467, 632, 665, 698
RsaNI GTAC 4 cut(s) 466, 631, 664, 697
SalI GTCGAC 2 cut(s) 152, 532
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 6 cut(s) 405, 553, 604, 729, 777, 835
Sau96I GGNCC 3 cut(s) 144, 372, 432
SchI GAGTC 6 cut(s) 134, 431, 520, 566, 593, 602
ScrFI CCNGG 7 cut(s) 38, 62, 118, 148, 149, 238, 326
SfaNI GCATC 1 cut(s) 18
SfoI GGCGCC 2 cut(s) 503, 820
SfuI TTCGAA 2 cut(s) 644, 677
SgrAI CRCCGGYG 3 cut(s) 504, 658, 724
SinI GGWCC 3 cut(s) 144, 372, 432
SmaI CCCGGG 1 cut(s) 149
Sse9I AATT 2 cut(s) 215, 568
SspDI GGCGCC 2 cut(s) 501, 818
SspMI CTAG 2 cut(s) 443, 773
StyD4I CCNGG 7 cut(s) 36, 60, 116, 146, 147, 236, 324
StyI CCWWGG 2 cut(s) 435, 786
TaaI ACNGT 1 cut(s) 295
TaiI ACGT 3 cut(s) 402, 453, 656
TaqI TCGA 9 cut(s) 123, 128, 153, 249, 533, 560, 644, 677, 710
TaqII GACCGA 1 cut(s) 519
TasI AATT 2 cut(s) 215, 568
TauI GCSGC 4 cut(s) 407, 606, 731, 779
TfiI GAWTC 2 cut(s) 329, 415
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 654
TseI GCWGC 2 cut(s) 552, 834
Tsp45I GTSAC 1 cut(s) 654
TspDTI ATGAA 3 cut(s) 17, 113, 770
TspGWI ACGGA 2 cut(s) 66, 526
TspMI CCCGGG 1 cut(s) 147
TspRI CASTG 1 cut(s) 24
Tth111I GACNNNGTC 3 cut(s) 158, 449, 529
VpaK11BI GGWCC 3 cut(s) 144, 372, 432
XapI RAATTY 2 cut(s) 215, 568
XmaI CCCGGG 1 cut(s) 147
XmiI GTMKAC 2 cut(s) 153, 533
XspI CTAG 2 cut(s) 443, 773
ZraI GACGTC 2 cut(s) 451, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.