MD01G1073700.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009207
Physical Location & Seq
Forward (+)
17921210 .. 17922022
813 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1073700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGATGATGAAGAAAGAAAACGGAGACCATCACGTGCACGTCTTGGAGATCAACCTCATCTCCGCCCAGGGCCTCAAGCCTCCCTCCGCCGCCCTCCGCCGCATGCAGACCTACGCCCTCGCCTGGGTCGACTCCGCCCACAAGATCCGCTCCCGGGTCGACAAGATCGGCGGCGAGAACCCCACGTGGAACGATCGGTTCCTCTTCAAGGTCCCGTCCGCATTTCTCTCAAGCGAGACCTCCCAGGTCTCCATCCAGATCTATGCCGTCGGCTGCTTCAGCGATCACCTCGTCGGCACCGTCCGACTTCTGATCAACAACTTTCTCGACGTCGCGTCCAAGGTTCCGTCGTTTACGGCCATCCAGATTCGGAGACCTTCTGGAAGATTCCAAGGCGTGTTGAATGTGGCCGCGATGGTGATCGACGGCTCGTATCTGGCTCCGGCGGTGAGCGAGTTGTCTGCGGTCGGGTACCGCGACCTGATGGGAAAGGGAGAAAGCTTCCGGCGCCGGCGACGCGACAGCATGAAGAGCAAATCGAGTGATTACCCCGGCGATTCGAAGGAAAACTCTTTCACTGAGTCGGCGGAGAACTCAGACGTCTGCGAGTCGGCGGCATCATCTCCGGCGACGCCATTACCGCCTCTGAAGGAGCTGAACACGATCACCGAATTGGCGGGAACAACAAGGCAGGTGTTGAAGGCTCGTCCAATGGACGGCTCGCGTTTCCTGTGCTGCTTGCTGACGCAGAGGAAGATGCAATACAGTCCGTCGGATGAGAACGCGGATGGAGCCCACAGCAGAGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

271

Amino Acids

29.81

Weight (kDa)

9.17

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 14 - 108 5.3e-11 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 682
AatII GACGTC 2 cut(s) 333, 603
Acc36I ACCTGC 1 cut(s) 682
Acc65I GGTACC 1 cut(s) 471
AccB1I GGYRCC 3 cut(s) 296, 471, 507
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 2 cut(s) 129, 159
AccII CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
AclWI GGATC 1 cut(s) 139
AcoI YGGCCR 2 cut(s) 357, 408
AcuI CTGAAG 2 cut(s) 262, 668
AcvI CACGTG 2 cut(s) 34, 186
AcyI GRCGYC 4 cut(s) 330, 508, 600, 632
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 208, 716
AgsI TTSAA 3 cut(s) 208, 403, 700
AhdI GACNNNNNGTC 1 cut(s) 334
AjiI CACGTC 1 cut(s) 40
AjnI CCWGG 3 cut(s) 66, 122, 243
AluBI AGCT 2 cut(s) 501, 655
AluI AGCT 2 cut(s) 501, 655
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 4 cut(s) 18, 230, 253, 367
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 3 cut(s) 70, 357, 408
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 2 cut(s) 273, 735
Asp718I GGTACC 1 cut(s) 471
AspLEI GCGC 1 cut(s) 510
AspS9I GGNCC 2 cut(s) 70, 211
AsuC2I CCSGG 3 cut(s) 154, 155, 552
AsuHPI GGTGA 4 cut(s) 278, 430, 460, 658
AsuII TTCGAA 1 cut(s) 560
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 39
BanI GGYRCC 3 cut(s) 296, 471, 507
BanII GRGCYC 1 cut(s) 796
BarI GAAGNNNNNNTAC 2 cut(s) 746, 778
BbrPI CACGTG 2 cut(s) 34, 186
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 260, 722
BccI CCATC 6 cut(s) 36, 260, 368, 409, 478, 782
BceAI ACGGC 4 cut(s) 251, 372, 442, 733
BcgI CGANNNNNNTGC 2 cut(s) 443, 477
BciT130I CCWGG 3 cut(s) 68, 124, 245
BclI TGATCA 1 cut(s) 312
BcnI CCSGG 3 cut(s) 154, 155, 552
BcoDI GTCTC 4 cut(s) 18, 230, 253, 367
BfoI RGCGCY 1 cut(s) 511
BfuAI ACCTGC 1 cut(s) 682
BglII AGATCT 1 cut(s) 258
BisI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
BlsI GCNGC 7 cut(s) 91, 101, 173, 275, 412, 616, 737
Bme1390I CCNGG 6 cut(s) 68, 124, 154, 155, 245, 552
Bme18I GGWCC 1 cut(s) 211
BmeRI GACNNNNNGTC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 2 cut(s) 70, 211
BmiI GGNNCC 8 cut(s) 200, 213, 298, 345, 441, 473, 509, 793
BmrFI CCNGG 6 cut(s) 68, 124, 154, 155, 245, 552
BmsI GCATC 2 cut(s) 626, 747
Bpu14I TTCGAA 1 cut(s) 560
BpuEI CTTGAG 2 cut(s) 59, 214
BpuMI CCSGG 3 cut(s) 154, 155, 552
BsaAI YACGTR 2 cut(s) 34, 186
BsaBI GATNNNNATC 1 cut(s) 419
BsaHI GRCGYC 4 cut(s) 330, 508, 600, 632
BsaI GGTCTC 4 cut(s) 18, 230, 253, 367
BsaJI CCNNGG 8 cut(s) 66, 67, 123, 153, 243, 339, 391, 550
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 124, 154, 208, 716
Bse118I RCCGGY 1 cut(s) 510
Bse8I GATNNNNATC 1 cut(s) 419
BseBI CCWGG 3 cut(s) 68, 124, 245
BseDI CCNNGG 8 cut(s) 66, 67, 123, 153, 243, 339, 391, 550
BseGI GGATG 4 cut(s) 252, 360, 781, 793
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 208, 716
BseMII CTCAG 2 cut(s) 570, 609
BseSI GKGCMC 1 cut(s) 39
BseXI GCAGC 2 cut(s) 260, 722
Bsh1236I CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
Bsh1285I CGRYCG 2 cut(s) 196, 468
BshFI GGCC 3 cut(s) 72, 359, 410
BshNI GGYRCC 3 cut(s) 296, 471, 507
BsiEI CGRYCG 2 cut(s) 196, 468
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
BslFI GGGAC 1 cut(s) 197
BslI CCNNNNNNNGG 5 cut(s) 123, 124, 154, 208, 716
BsmAI GTCTC 4 cut(s) 18, 230, 253, 367
BsmFI GGGAC 1 cut(s) 197
BsnI GGCC 3 cut(s) 72, 359, 410
Bso31I GGTCTC 4 cut(s) 18, 230, 253, 367
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 39, 796
Bsp143I GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
BspANI GGCC 3 cut(s) 72, 359, 410
BspCNI CTCAG 2 cut(s) 571, 608
BspFNI CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
BspLI GGNNCC 8 cut(s) 200, 213, 298, 345, 441, 473, 509, 793
BspMI ACCTGC 1 cut(s) 682
BspPI GGATC 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 524
BspT104I TTCGAA 1 cut(s) 560
BspT107I GGYRCC 3 cut(s) 296, 471, 507
BspTNI GGTCTC 4 cut(s) 18, 230, 253, 367
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 8 cut(s) 66, 67, 123, 153, 243, 339, 391, 550
BssMI GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
BssNI GRCGYC 4 cut(s) 330, 508, 600, 632
BssT1I CCWWGG 2 cut(s) 339, 391
Bst2UI CCWGG 3 cut(s) 68, 124, 245
Bst4CI ACNGT 2 cut(s) 301, 767
Bst6I CTCTTC 2 cut(s) 209, 524
BstACI GRCGYC 4 cut(s) 330, 508, 600, 632
BstBAI YACGTR 2 cut(s) 34, 186
BstBI TTCGAA 1 cut(s) 560
BstC8I GCNNGC 4 cut(s) 104, 512, 722, 740
BstDEI CTNAG 2 cut(s) 579, 595
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 4 cut(s) 252, 360, 781, 793
BstFNI CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
BstH2I RGCGCY 1 cut(s) 511
BstHHI GCGC 1 cut(s) 510
BstKTI GATC 9 cut(s) 51, 147, 168, 196, 261, 286, 315, 423, 666
BstMAI GTCTC 4 cut(s) 18, 230, 253, 367
BstMBI GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
BstMCI CGRYCG 2 cut(s) 196, 468
BstMWI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
BstNI CCWGG 3 cut(s) 68, 124, 245
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 6 cut(s) 66, 122, 152, 153, 243, 550
BstSLI GKGCMC 1 cut(s) 39
BstUI CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
BstV1I GCAGC 2 cut(s) 260, 722
BstX2I RGATCY 2 cut(s) 144, 258
BstYI RGATCY 2 cut(s) 144, 258
BsuRI GGCC 3 cut(s) 72, 359, 410
BtgZI GCGATG 1 cut(s) 428
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 4 cut(s) 252, 360, 781, 793
BtsIMutI CAGTG 1 cut(s) 576
BveI ACCTGC 1 cut(s) 682
Cac8I GCNNGC 4 cut(s) 104, 512, 722, 740
CfoI GCGC 1 cut(s) 510
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 2 cut(s) 70, 211
Cfr9I CCCGGG 1 cut(s) 153
CseI GACGC 4 cut(s) 324, 525, 640, 754
Csp6I GTAC 1 cut(s) 472
CviAII CATG 2 cut(s) 103, 526
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 579, 595
DinI GGCGCC 1 cut(s) 509
DpnI GATC 9 cut(s) 50, 146, 167, 195, 260, 285, 314, 422, 665
DpnII GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 357, 408
Eam1104I CTCTTC 2 cut(s) 209, 524
Eam1105I GACNNNNNGTC 1 cut(s) 334
EarI CTCTTC 2 cut(s) 209, 524
EciI GGCGGA 5 cut(s) 52, 76, 86, 124, 602
Eco130I CCWWGG 2 cut(s) 339, 391
Eco24I GRGCYC 1 cut(s) 796
Eco31I GGTCTC 4 cut(s) 18, 230, 253, 367
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 262, 668
Eco72I CACGTG 2 cut(s) 34, 186
Eco88I CYCGRG 1 cut(s) 153
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 2 cut(s) 70, 211
EcoRII CCWGG 3 cut(s) 66, 122, 243
EcoT14I CCWWGG 2 cut(s) 339, 391
EcoT38I GRGCYC 1 cut(s) 796
EgeI GGCGCC 1 cut(s) 509
EheI GGCGCC 1 cut(s) 509
ErhI CCWWGG 2 cut(s) 339, 391
FaeI CATG 2 cut(s) 106, 529
FaiI YATR 3 cut(s) 104, 264, 527
FaqI GGGAC 1 cut(s) 197
FatI CATG 2 cut(s) 102, 525
FauI CCCGC 1 cut(s) 670
FbaI TGATCA 1 cut(s) 312
FblI GTMKAC 2 cut(s) 129, 159
Fnu4HI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
FokI GGATG 4 cut(s) 239, 347, 788, 800
FriOI GRGCYC 1 cut(s) 796
Fsp4HI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
GlaI GCGC 1 cut(s) 509
GluI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
HaeII RGCGCY 1 cut(s) 511
HaeIII GGCC 3 cut(s) 72, 359, 410
HapII CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
HgaI GACGC 4 cut(s) 324, 525, 640, 754
HhaI GCGC 1 cut(s) 510
Hin1I GRCGYC 4 cut(s) 330, 508, 600, 632
Hin1II CATG 2 cut(s) 106, 529
Hin6I GCGC 1 cut(s) 508
HinP1I GCGC 1 cut(s) 508
HincII GTYRAC 2 cut(s) 130, 160
HindII GTYRAC 2 cut(s) 130, 160
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 6 cut(s) 131, 367, 387, 557, 581, 608
HpaII CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
HphI GGTGA 4 cut(s) 278, 430, 460, 658
Hpy166II GTNNAC 4 cut(s) 37, 130, 160, 354
Hpy188I TCNGA 6 cut(s) 305, 312, 372, 598, 648, 775
Hpy188III TCNNGA 4 cut(s) 256, 326, 364, 381
Hpy8I GTNNAC 4 cut(s) 37, 130, 160, 354
Hpy99I CGWCG 9 cut(s) 272, 296, 332, 335, 352, 428, 519, 634, 775
HpyAV CCTTC 4 cut(s) 387, 556, 643, 694
HpyCH4III ACNGT 2 cut(s) 301, 767
HpyCH4IV ACGT 5 cut(s) 33, 39, 185, 330, 600
HpyCH4V TGCA 3 cut(s) 37, 106, 760
HpyF10VI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
HpyF3I CTNAG 2 cut(s) 579, 595
HpySE526I ACGT 5 cut(s) 33, 39, 185, 330, 600
Hsp92I GRCGYC 4 cut(s) 330, 508, 600, 632
Hsp92II CATG 2 cut(s) 106, 529
HspAI GCGC 1 cut(s) 508
KasI GGCGCC 1 cut(s) 507
KpnI GGTACC 1 cut(s) 475
KroI GCCGGC 1 cut(s) 510
KroNI GCCGGC 1 cut(s) 512
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
LguI GCTCTTC 1 cut(s) 524
LmnI GCTCC 4 cut(s) 155, 445, 652, 791
Lsp1109I GCAGC 2 cut(s) 260, 722
LweI GCATC 2 cut(s) 626, 747
MaeII ACGT 5 cut(s) 33, 39, 185, 330, 600
MalI GATC 9 cut(s) 50, 146, 167, 195, 260, 285, 314, 422, 665
MbiI CCGCTC 1 cut(s) 150
MboI GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
MboII GAAGA 5 cut(s) 22, 196, 396, 541, 766
MflI RGATCY 2 cut(s) 144, 258
MhlI GDGCHC 2 cut(s) 39, 796
MluCI AATT 1 cut(s) 671
Mly113I GGCGCC 1 cut(s) 508
MlyI GAGTC 3 cut(s) 125, 590, 617
MmeI TCCRAC 2 cut(s) 328, 753
MreI CGCCGGCG 1 cut(s) 510
MroNI GCCGGC 1 cut(s) 510
MspI CCGG 6 cut(s) 154, 443, 505, 511, 552, 626
MspR9I CCNGG 6 cut(s) 68, 124, 154, 155, 245, 552
MvaI CCWGG 3 cut(s) 68, 124, 245
MvnI CGCG 6 cut(s) 335, 413, 477, 519, 724, 785
MwoI GCNNNNNNNGC 6 cut(s) 279, 507, 516, 531, 640, 791
NaeI GCCGGC 1 cut(s) 512
NarI GGCGCC 1 cut(s) 508
NciI CCSGG 3 cut(s) 154, 155, 552
NdeII GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
NgoMIV GCCGGC 1 cut(s) 510
NlaIII CATG 2 cut(s) 106, 529
NlaIV GGNNCC 8 cut(s) 200, 213, 298, 345, 441, 473, 509, 793
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 560
PaeI GCATGC 1 cut(s) 106
PaqCI CACCTGC 1 cut(s) 682
PasI CCCWGGG 1 cut(s) 67
PciSI GCTCTTC 1 cut(s) 524
PcsI WCGNNNNNNNCGW 3 cut(s) 126, 297, 474
PdiI GCCGGC 1 cut(s) 512
PfeI GAWTC 3 cut(s) 367, 387, 557
PkrI GCNGC 7 cut(s) 91, 101, 173, 275, 412, 616, 737
Ple19I CGATCG 1 cut(s) 196
PleI GAGTC 3 cut(s) 125, 589, 616
PluTI GGCGCC 1 cut(s) 511
PmaCI CACGTG 2 cut(s) 34, 186
PmlI CACGTG 2 cut(s) 34, 186
PpsI GAGTC 3 cut(s) 125, 589, 616
Ppu21I YACGTR 2 cut(s) 34, 186
PpuMI RGGWCCY 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 3 cut(s) 66, 122, 243
PspCI CACGTG 2 cut(s) 34, 186
PspGI CCWGG 3 cut(s) 66, 122, 243
PspN4I GGNNCC 8 cut(s) 200, 213, 298, 345, 441, 473, 509, 793
PspPI GGNCC 2 cut(s) 70, 211
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 2 cut(s) 144, 258
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SalI GTCGAC 2 cut(s) 128, 158
SapI GCTCTTC 1 cut(s) 524
SatI GCNGC 7 cut(s) 90, 100, 172, 274, 411, 615, 736
Sau3AI GATC 9 cut(s) 48, 144, 165, 193, 258, 283, 312, 420, 663
Sau96I GGNCC 2 cut(s) 70, 211
SchI GAGTC 3 cut(s) 125, 590, 617
ScrFI CCNGG 6 cut(s) 68, 124, 154, 155, 245, 552
SduI GDGCHC 2 cut(s) 39, 796
SfaNI GCATC 2 cut(s) 626, 747
SfoI GGCGCC 1 cut(s) 509
SfuI TTCGAA 1 cut(s) 560
SgrAI CRCCGGYG 1 cut(s) 510
SinI GGWCC 1 cut(s) 211
SmaI CCCGGG 1 cut(s) 155
SmlI CTYRAG 2 cut(s) 74, 229
SmoI CTYRAG 2 cut(s) 74, 229
SphI GCATGC 1 cut(s) 106
Sse9I AATT 1 cut(s) 671
SspDI GGCGCC 1 cut(s) 507
StyD4I CCNGG 6 cut(s) 66, 122, 152, 153, 243, 550
StyI CCWWGG 2 cut(s) 339, 391
TaaI ACNGT 2 cut(s) 301, 767
TaiI ACGT 5 cut(s) 36, 42, 188, 333, 603
TaqI TCGA 6 cut(s) 129, 159, 327, 423, 539, 560
TasI AATT 1 cut(s) 671
TauI GCSGC 5 cut(s) 92, 102, 174, 413, 617
TfiI GAWTC 3 cut(s) 367, 387, 557
TscAI CASTG 1 cut(s) 583
TseI GCWGC 2 cut(s) 273, 735
TspDTI ATGAA 2 cut(s) 23, 542
TspGWI ACGGA 3 cut(s) 36, 336, 759
TspMI CCCGGG 1 cut(s) 153
TspRI CASTG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 206
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 129, 159
ZraI GACGTC 2 cut(s) 331, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.