AT4G06676

Etoposide-induced protein 2.4 (EI24)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008616
Physical Location & Seq
Forward (+)
3898811 .. 3901990
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G06676.2

Sequence Viewer

Length: 963 bp
ATGAGATCAAAATCGAAGCAGGTGTTGCTACTATGGCTTGAGGGTTTTCGAGAAGCTTGTTCTCTTCACCGTGTTGTCATTCTCTGTCTCAGGTCGAGAAAGCTTCTCTTAAGAACTGGGCAGTGTTTTCTTTTGAATGGTTTGATTTTCTTAGGAAGTTTAGGTGTGTTTAAGTGGTTTATAGATCCATCCCTTCAGTGGATATTACCTGATCAGTGCTCTCCCCTCACTTCTCAAGAATTTTGTTCGTATGGTGGATTTTACGCCTTTCTACGTGGTGGACTTTTGCAGCTCTTTTACGTTTTTTGGTTCTATCCTCTGTACATGCTCAGCTTCATCTTGAGTAACATCTGGTATAACGATATTGCAAAGCATGGATTCGAGGCAATTGAGATATCTGACTTAAACTCAGCTGAAGCATTAAGACAAGGTGAAGCTCTGGCGTCCCTGAATATGGCAAATGCTGAAAGGCCTTCTGGTCTTGGAGGGGTGATGATCGGTATAGGAGAGCAGGTGTACTCGATACTTCTCCTGACATTTTTTTTTCTTGAGGTTTGTGTTGTTGGTGTTATACCATACATTGGGAAGATATTGAATTTCTTACTTCTTTCATGGATGTATGCCTACTATTGTTACGAATATAAGTGGAACTTCTCGGGGATTTCTCTGAAGAAAAGGCTGGACTTCTTTCAATCTAACTGGGCATTCTTTGCTGGTTTTGGAAGCCCCTGCGTGTTGGCCATTTTCTTTCTCTCGCCTCTTGTGAGCGGAGCGCTTATGGCCATCTTATTTCCATTGTTTGTCTTAACTGCTACAGGATCAGGACCTGAAAAATTGATTGGGGCCCCGAGAAGAACGTGGAAATGTGCAGGTTTAGGAAAGCTTCCAATATTCTATATTGCTGACACTCTCTCGATGTTGGCGTTGTCTATCTTCCGGTTGGAGTCCCCTCACGAGAATTAA

Protein Analysis

320

Amino Acids

36.21

Weight (kDa)

8.67

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 22 - 265 3.2e-30 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 10, 502
Acc36I ACCTGC 3 cut(s) 10, 502, 860
AccB7I CCANNNNNTGG 1 cut(s) 581
AccBSI CCGCTC 1 cut(s) 768
AciI CCGC 1 cut(s) 768
AclWI GGATC 2 cut(s) 179, 826
AcoI YGGCCR 2 cut(s) 738, 780
AcsI RAATTY 2 cut(s) 239, 595
AcuI CTGAAG 3 cut(s) 179, 435, 689
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 2 cut(s) 323, 518
AfeI AGCGCT 1 cut(s) 774
AfiI CCNNNNNNNGG 3 cut(s) 198, 454, 581
AflII CTTAAG 1 cut(s) 109
AgsI TTSAA 3 cut(s) 136, 595, 692
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AluBI AGCT 7 cut(s) 56, 103, 292, 333, 413, 437, 883
AluI AGCT 7 cut(s) 56, 103, 292, 333, 413, 437, 883
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 2 cut(s) 179, 826
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 2 cut(s) 655, 847
Aor51HI AGCGCT 1 cut(s) 774
AoxI GGCC 4 cut(s) 470, 738, 780, 843
ApaI GGGCCC 1 cut(s) 847
ApeKI GCWGC 1 cut(s) 289
ApoI RAATTY 2 cut(s) 239, 595
AspLEI GCGC 1 cut(s) 775
AspS9I GGNCC 3 cut(s) 824, 843, 844
AsuHPI GGTGA 3 cut(s) 59, 443, 502
AvaI CYCGRG 2 cut(s) 655, 847
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 847
BalI TGGCCA 2 cut(s) 740, 782
BanII GRGCYC 1 cut(s) 847
BauI CACGAG 1 cut(s) 953
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 301
BccI CCATC 2 cut(s) 196, 791
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 92
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 776
BfrI CTTAAG 1 cut(s) 109
BfuAI ACCTGC 3 cut(s) 10, 502, 860
BisI GCNGC 1 cut(s) 290
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 1 cut(s) 291
Bme18I GGWCC 1 cut(s) 824
BmeT110I CYCGRG 2 cut(s) 655, 847
BmgT120I GGNCC 3 cut(s) 824, 843, 844
BmiI GGNNCC 3 cut(s) 844, 845, 846
BmrI ACTGGG 2 cut(s) 126, 709
BmuI ACTGGG 2 cut(s) 126, 709
Bpu1102I GCTNAGC 1 cut(s) 329
BpuEI CTTGAG 4 cut(s) 59, 219, 361, 569
BsaAI YACGTR 1 cut(s) 275
BsaBI GATNNNNATC 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 443
BsaWI WCCGGW 1 cut(s) 936
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 454, 581
Bse1I ACTGG 2 cut(s) 121, 704
Bse8I GATNNNNATC 1 cut(s) 10
BseGI GGATG 2 cut(s) 188, 621
BseJI GATNNNNATC 1 cut(s) 10
BseLI CCNNNNNNNGG 3 cut(s) 198, 454, 581
BseMII CTCAG 3 cut(s) 103, 343, 423
BseNI ACTGG 2 cut(s) 121, 704
BseSI GKGCMC 1 cut(s) 847
BseXI GCAGC 1 cut(s) 301
BsgI GTGCAG 1 cut(s) 888
BshFI GGCC 4 cut(s) 472, 740, 782, 845
BsiHKAI GWGCWC 1 cut(s) 221
BsiHKCI CYCGRG 2 cut(s) 655, 847
BsiSI CCGG 1 cut(s) 937
BslFI GGGAC 2 cut(s) 430, 931
BslI CCNNNNNNNGG 3 cut(s) 198, 454, 581
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 2 cut(s) 430, 931
BsmI GAATGC 1 cut(s) 704
BsnI GGCC 4 cut(s) 472, 740, 782, 845
BsoBI CYCGRG 2 cut(s) 655, 847
Bsp120I GGGCCC 1 cut(s) 843
Bsp1286I GDGCHC 2 cut(s) 221, 847
Bsp1407I TGTACA 1 cut(s) 321
Bsp143I GATC 5 cut(s) 5, 184, 211, 495, 818
Bsp1720I GCTNAGC 1 cut(s) 329
BspACI CCGC 1 cut(s) 768
BspANI GGCC 4 cut(s) 472, 740, 782, 845
BspCNI CTCAG 3 cut(s) 102, 342, 422
BspLI GGNNCC 3 cut(s) 844, 845, 846
BspMI ACCTGC 3 cut(s) 10, 502, 860
BspPI GGATC 2 cut(s) 179, 826
BspTI CTTAAG 1 cut(s) 109
BsrBI CCGCTC 1 cut(s) 768
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 2 cut(s) 121, 704
BssMI GATC 5 cut(s) 5, 184, 211, 495, 818
BssNI GRCGYC 1 cut(s) 443
BssSI CACGAG 1 cut(s) 953
Bst2BI CACGAG 1 cut(s) 953
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 109
BstAPI GCANNNNNTGC 2 cut(s) 25, 710
BstAUI TGTACA 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 275
BstDEI CTNAG 4 cut(s) 89, 151, 329, 409
BstF5I GGATG 2 cut(s) 188, 621
BstH2I RGCGCY 1 cut(s) 776
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 5 cut(s) 8, 187, 214, 498, 821
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 5, 184, 211, 495, 818
BstMWI GCNNNNNNNGC 4 cut(s) 25, 34, 710, 779
BstNSI RCATGY 1 cut(s) 328
BstSFI CTRYAG 1 cut(s) 813
BstSLI GKGCMC 1 cut(s) 847
BstV1I GCAGC 1 cut(s) 301
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 4 cut(s) 472, 740, 782, 845
BtsCI GGATG 2 cut(s) 188, 621
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 3 cut(s) 128, 203, 221
BveI ACCTGC 3 cut(s) 10, 502, 860
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 1 cut(s) 775
Cfr13I GGNCC 3 cut(s) 824, 843, 844
CseI GACGC 1 cut(s) 432
Csp6I GTAC 2 cut(s) 322, 517
CviAII CATG 3 cut(s) 325, 374, 612
CviQI GTAC 2 cut(s) 322, 517
DdeI CTNAG 4 cut(s) 89, 151, 329, 409
DpnI GATC 5 cut(s) 7, 186, 213, 497, 820
DpnII GATC 5 cut(s) 5, 184, 211, 495, 818
EaeI YGGCCR 2 cut(s) 738, 780
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 847
Eco32I GATATC 1 cut(s) 396
Eco47I GGWCC 1 cut(s) 824
Eco47III AGCGCT 1 cut(s) 774
Eco57I CTGAAG 3 cut(s) 179, 435, 689
Eco88I CYCGRG 2 cut(s) 655, 847
EcoO109I RGGNCCY 3 cut(s) 824, 843, 844
EcoRV GATATC 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 847
FaeI CATG 3 cut(s) 328, 377, 615
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 635, 667
FaqI GGGAC 2 cut(s) 430, 931
FatI CATG 3 cut(s) 324, 373, 611
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 290
FokI GGATG 2 cut(s) 175, 628
FriOI GRGCYC 1 cut(s) 847
Fsp4HI GCNGC 1 cut(s) 290
GlaI GCGC 1 cut(s) 774
GluI GCNGC 1 cut(s) 290
HaeII RGCGCY 1 cut(s) 776
HaeIII GGCC 4 cut(s) 472, 740, 782, 845
HapII CCGG 1 cut(s) 937
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 775
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 3 cut(s) 328, 377, 615
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HindIII AAGCTT 3 cut(s) 54, 101, 881
HinfI GANTC 2 cut(s) 378, 944
HpaII CCGG 1 cut(s) 937
HphI GGTGA 3 cut(s) 59, 443, 502
Hpy166II GTNNAC 2 cut(s) 281, 517
Hpy188I TCNGA 2 cut(s) 400, 669
Hpy188III TCNNGA 9 cut(s) 50, 96, 236, 340, 532, 548, 822, 913, 953
Hpy8I GTNNAC 2 cut(s) 281, 517
HpyAV CCTTC 2 cut(s) 203, 483
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4IV ACGT 3 cut(s) 274, 300, 857
HpyCH4V TGCA 3 cut(s) 289, 368, 869
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 34, 710, 779
HpyF3I CTNAG 4 cut(s) 89, 151, 329, 409
HpySE526I ACGT 3 cut(s) 274, 300, 857
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 3 cut(s) 328, 377, 615
HspAI GCGC 1 cut(s) 773
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 5 cut(s) 5, 184, 211, 495, 818
LmnI GCTCC 1 cut(s) 770
Lsp1109I GCAGC 1 cut(s) 301
MaeII ACGT 3 cut(s) 274, 300, 857
MaeIII GTNAC 2 cut(s) 344, 632
MalI GATC 5 cut(s) 7, 186, 213, 497, 820
MbiI CCGCTC 1 cut(s) 768
MboI GATC 5 cut(s) 5, 184, 211, 495, 818
MboII GAAGA 5 cut(s) 56, 598, 682, 864, 925
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 221, 847
MlsI TGGCCA 2 cut(s) 740, 782
MluCI AATT 5 cut(s) 239, 387, 595, 833, 958
MluNI TGGCCA 2 cut(s) 740, 782
MlyI GAGTC 1 cut(s) 953
MmeI TCCRAC 1 cut(s) 921
MnlI CCTC 8 cut(s) 34, 236, 327, 376, 479, 544, 768, 960
Mox20I TGGCCA 2 cut(s) 740, 782
MscI TGGCCA 2 cut(s) 740, 782
MseI TTAA 6 cut(s) 110, 171, 404, 422, 806, 961
Msp20I TGGCCA 2 cut(s) 740, 782
MspA1I CMGCKG 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 109
MspI CCGG 1 cut(s) 937
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 704
MwoI GCNNNNNNNGC 4 cut(s) 25, 34, 710, 779
NdeII GATC 5 cut(s) 5, 184, 211, 495, 818
NlaIII CATG 3 cut(s) 328, 377, 615
NlaIV GGNNCC 3 cut(s) 844, 845, 846
NspI RCATGY 1 cut(s) 328
PaqCI CACCTGC 2 cut(s) 10, 502
PceI AGGCCT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 920
PctI GAATGC 1 cut(s) 704
PfeI GAWTC 1 cut(s) 378
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 1 cut(s) 291
PleI GAGTC 1 cut(s) 952
PpsI GAGTC 1 cut(s) 952
Ppu21I YACGTR 1 cut(s) 275
PpuMI RGGWCCY 1 cut(s) 824
Psp5II RGGWCCY 1 cut(s) 824
PspN4I GGNNCC 3 cut(s) 844, 845, 846
PspOMI GGGCCC 1 cut(s) 843
PspPI GGNCC 3 cut(s) 824, 843, 844
PspPPI RGGWCCY 1 cut(s) 824
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 2 cut(s) 323, 518
RsaNI GTAC 2 cut(s) 322, 517
SaqAI TTAA 6 cut(s) 110, 171, 404, 422, 806, 961
SatI GCNGC 1 cut(s) 290
Sau3AI GATC 5 cut(s) 5, 184, 211, 495, 818
Sau96I GGNCC 3 cut(s) 824, 843, 844
SchI GAGTC 1 cut(s) 953
SduI GDGCHC 2 cut(s) 221, 847
SfcI CTRYAG 1 cut(s) 813
SinI GGWCC 1 cut(s) 824
SmlI CTYRAG 5 cut(s) 38, 109, 234, 340, 548
SmoI CTYRAG 5 cut(s) 38, 109, 234, 340, 548
Sse9I AATT 5 cut(s) 239, 387, 595, 833, 958
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 1 cut(s) 768
SspI AATATT 1 cut(s) 891
StuI AGGCCT 1 cut(s) 472
TaaI ACNGT 1 cut(s) 71
TaiI ACGT 3 cut(s) 277, 303, 860
TaqI TCGA 6 cut(s) 14, 49, 95, 381, 521, 914
TasI AATT 5 cut(s) 239, 387, 595, 833, 958
TatI WGTACW 2 cut(s) 321, 516
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 6 cut(s) 110, 171, 404, 422, 806, 961
Tru9I TTAA 6 cut(s) 110, 171, 404, 422, 806, 961
TscAI CASTG 3 cut(s) 128, 203, 221
TseI GCWGC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 325, 600
TspRI CASTG 3 cut(s) 128, 203, 221
Van91I CCANNNNNTGG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 2 cut(s) 239, 595
XceI RCATGY 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.