MD08G1240100.v1.1

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008616
Physical Location & Seq
Reverse (-)
30637733 .. 30645203
7471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1240100.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGAAGCTAAGAAGCCATGGATAAACGCTTTCAGAGCAAAATCAAAGCAAGCCACGCTGCTTTGGCTCGAGGGCTTCCGAGAGGCCTGCTGCCTTCACCGCGTTCTCATCCTCTGCCACCGGTCGAGAAAGCTTATGATTCGAACAGGACAGTGTTTTCTATTAAACGGCTTCATTTTCTTAGGAAGTATAATTATCCTTAACTCAGTCATCATCCCAACTCTACACTGGATATTACCTGATAGATGCCCAGAATATAGTTCTCAAGAACTCTGCTCATTTGGTGGTATATTGAAATTTTATTCCTTCTTGCGGCAGGGACTTGTACAACTCTTTTATGTAACTTGGTTCTACCCGCTATACGTATTCAGCATTATCCTGAGCAACATCTGGTACAACGACATTGCTAAGTATGGATTTTCTTCAATGGGGAGGTCCGCGCCAACCACATTGGATCCTGTCAGACAAAATGAGGCATTGGTCTTACAAAATATTGTTGGTCCGGAAAGACCTGCTGGCCTGGGAGGGGTCATAATTGGCATAGGAGAGCAGGTGTATTCAGTACTTCTTTTGAGTTGTTTCTTCCTAGAGGTATATGTGACAGGATTTATACCGTATGTTGGGATGGCACTAAATTTTCTGCTTCTGTCCTGGATGTATGCTTATTACTGTTTCGAGTACAAATGGAATTTTTCTGAAGTTCGTCTTGAAAAACGGCTGGATTTCTTTGAATCTAACTGGGCTTTTTTTGCTGGTTTTGGGAGCCCTTGCGTTCTGCCTATACTCTTTTTCTCACCTCTTGTGAGCTACGGATTTATGGCAATTCTCTTTCCATTGTTTGTTTTGACAGCAACGGGCTCAGAGGCTGAGCAAGTTATTTCTTCTCAAAGGACAAAATGGGAAGGTGCTGGGTTGGGAAAGCTTCCAATATTTAATGCTGCAGATAACCTATCGATGCGGGTTTTGTCTCTGTTTCCCCTGGAATCCCAGGAGTTACTTCAGAAAAAGAAAGATCGCTAA

Protein Analysis

340

Amino Acids

38.8

Weight (kDa)

8.78

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 31 - 278 8.3e-28 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 2 cut(s) 520, 541
AccII CGCG 2 cut(s) 102, 440
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 5 cut(s) 100, 313, 356, 438, 958
AclWI GGATC 2 cut(s) 449, 462
AcsI RAATTY 3 cut(s) 296, 634, 688
AcuI CTGAAG 2 cut(s) 717, 983
AfaI GTAC 4 cut(s) 327, 395, 564, 680
AfiI CCNNNNNNNGG 3 cut(s) 312, 526, 620
AgeI ACCGGT 1 cut(s) 120
AgsI TTSAA 4 cut(s) 295, 426, 710, 731
AjnI CCWGG 4 cut(s) 519, 650, 978, 987
AluBI AGCT 4 cut(s) 8, 133, 807, 922
AluI AGCT 4 cut(s) 8, 133, 807, 922
Alw26I GTCTC 1 cut(s) 972
AlwI GGATC 2 cut(s) 449, 462
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 68
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 84, 517
ApeKI GCWGC 3 cut(s) 58, 90, 938
ApoI RAATTY 3 cut(s) 296, 634, 688
AsiGI ACCGGT 1 cut(s) 120
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 2 cut(s) 435, 500
AsuHPI GGTGA 2 cut(s) 89, 786
AsuII TTCGAA 1 cut(s) 142
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 2 cut(s) 435, 500
BamHI GGATCC 1 cut(s) 454
BanII GRGCYC 2 cut(s) 767, 860
BbvI GCAGC 3 cut(s) 45, 77, 925
BccI CCATC 1 cut(s) 619
BceAI ACGGC 2 cut(s) 184, 731
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 4 cut(s) 521, 652, 980, 989
BcoDI GTCTC 1 cut(s) 972
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 939
BfuAI ACCTGC 2 cut(s) 520, 541
BisI GCNGC 4 cut(s) 59, 91, 314, 939
BlpI GCTNAGC 1 cut(s) 867
BlsI GCNGC 4 cut(s) 60, 92, 315, 940
BmcAI AGTACT 1 cut(s) 564
Bme1390I CCNGG 4 cut(s) 521, 652, 980, 989
Bme18I GGWCC 2 cut(s) 435, 500
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 435, 500
BmiI GGNNCC 2 cut(s) 456, 764
BmrFI CCNGG 4 cut(s) 521, 652, 980, 989
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 2 cut(s) 236, 945
BmuI ACTGGG 1 cut(s) 748
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 867
Bpu14I TTCGAA 1 cut(s) 142
BpuEI CTTGAG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 953
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 4 cut(s) 17, 520, 978, 987
BsaWI WCCGGW 2 cut(s) 120, 502
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 3 cut(s) 312, 526, 620
Bse118I RCCGGY 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 233, 743
Bse3DI GCAATG 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 4 cut(s) 521, 652, 980, 989
BseCI ATCGAT 1 cut(s) 953
BseDI CCNNGG 4 cut(s) 17, 520, 978, 987
BseGI GGATG 4 cut(s) 108, 213, 630, 660
BseLI CCNNNNNNNGG 3 cut(s) 312, 526, 620
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 4 cut(s) 219, 371, 858, 873
BseNI ACTGG 2 cut(s) 233, 743
BseXI GCAGC 3 cut(s) 45, 77, 925
BseYI CCCAGC 1 cut(s) 908
Bsh1236I CGCG 2 cut(s) 102, 440
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 86, 519
BshTI ACCGGT 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 953
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 2 cut(s) 121, 503
BslFI GGGAC 1 cut(s) 333
BslI CCNNNNNNNGG 3 cut(s) 312, 526, 620
BsmAI GTCTC 1 cut(s) 972
BsmFI GGGAC 1 cut(s) 333
BsnI GGCC 2 cut(s) 86, 519
BsoBI CYCGRG 1 cut(s) 68
Bsp119I TTCGAA 1 cut(s) 142
Bsp1286I GDGCHC 2 cut(s) 767, 860
Bsp13I TCCGGA 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 325
Bsp143I GATC 2 cut(s) 454, 1012
Bsp1720I GCTNAGC 1 cut(s) 867
Bsp19I CCATGG 1 cut(s) 17
BspACI CCGC 5 cut(s) 100, 313, 356, 438, 958
BspANI GGCC 2 cut(s) 86, 519
BspCNI CTCAG 4 cut(s) 218, 372, 859, 872
BspDI ATCGAT 1 cut(s) 953
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 2 cut(s) 102, 440
BspLI GGNNCC 2 cut(s) 456, 764
BspMAI CTGCAG 1 cut(s) 943
BspMI ACCTGC 2 cut(s) 520, 541
BspPI GGATC 2 cut(s) 449, 462
BspT104I TTCGAA 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 120
BsrGI TGTACA 1 cut(s) 325
BsrI ACTGG 2 cut(s) 233, 743
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 4 cut(s) 17, 520, 978, 987
BssMI GATC 2 cut(s) 454, 1012
BssT1I CCWWGG 1 cut(s) 17
Bst2UI CCWGG 4 cut(s) 521, 652, 980, 989
Bst4CI ACNGT 3 cut(s) 153, 615, 671
BstAUI TGTACA 1 cut(s) 325
BstBAI YACGTR 1 cut(s) 364
BstBI TTCGAA 1 cut(s) 142
BstC8I GCNNGC 3 cut(s) 51, 88, 517
BstDEI CTNAG 7 cut(s) 9, 181, 205, 380, 408, 859, 867
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 4 cut(s) 108, 213, 630, 660
BstFNI CGCG 2 cut(s) 102, 440
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 457, 1015
BstMAI GTCTC 1 cut(s) 972
BstMBI GATC 2 cut(s) 454, 1012
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 5 cut(s) 35, 55, 64, 99, 749
BstNI CCWGG 4 cut(s) 521, 652, 980, 989
BstSCI CCNGG 4 cut(s) 519, 650, 978, 987
BstSFI CTRYAG 1 cut(s) 939
BstSNI TACGTA 1 cut(s) 364
BstUI CGCG 2 cut(s) 102, 440
BstV1I GCAGC 3 cut(s) 45, 77, 925
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 953
BsuRI GGCC 2 cut(s) 86, 519
BsuTUI ATCGAT 1 cut(s) 953
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 4 cut(s) 108, 213, 630, 660
BtsIMutI CAGTG 2 cut(s) 158, 226
BveI ACCTGC 2 cut(s) 520, 541
Cac8I GCNNGC 3 cut(s) 51, 88, 517
CaiI CAGNNNCTG 1 cut(s) 866
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 435, 500
ClaI ATCGAT 1 cut(s) 953
Csp6I GTAC 4 cut(s) 326, 394, 563, 679
CspAI ACCGGT 1 cut(s) 120
CviAII CATG 1 cut(s) 18
CviQI GTAC 4 cut(s) 326, 394, 563, 679
DdeI CTNAG 7 cut(s) 9, 181, 205, 380, 408, 859, 867
DpnI GATC 2 cut(s) 456, 1014
DpnII GATC 2 cut(s) 454, 1012
Eco105I TACGTA 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 17
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 2 cut(s) 767, 860
Eco47I GGWCC 2 cut(s) 435, 500
Eco57I CTGAAG 2 cut(s) 717, 983
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 4 cut(s) 519, 650, 978, 987
EcoT14I CCWWGG 1 cut(s) 17
EcoT38I GRGCYC 2 cut(s) 767, 860
ErhI CCWWGG 1 cut(s) 17
FaeI CATG 1 cut(s) 21
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FaqI GGGAC 1 cut(s) 333
FatI CATG 1 cut(s) 17
FauI CCCGC 2 cut(s) 363, 951
Fnu4HI GCNGC 4 cut(s) 59, 91, 314, 939
FokI GGATG 4 cut(s) 95, 200, 637, 667
FriOI GRGCYC 2 cut(s) 767, 860
Fsp4HI GCNGC 4 cut(s) 59, 91, 314, 939
FspBI CTAG 1 cut(s) 587
GlaI GCGC 1 cut(s) 441
GluI GCNGC 4 cut(s) 59, 91, 314, 939
GsaI CCCAGC 1 cut(s) 912
HaeIII GGCC 2 cut(s) 86, 519
HapII CCGG 2 cut(s) 121, 503
HhaI GCGC 1 cut(s) 442
Hin1II CATG 1 cut(s) 21
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 2 cut(s) 131, 920
HinfI GANTC 3 cut(s) 139, 731, 983
HpaII CCGG 2 cut(s) 121, 503
HphI GGTGA 2 cut(s) 89, 786
Hpy188I TCNGA 6 cut(s) 35, 80, 464, 697, 862, 1002
Hpy188III TCNNGA 5 cut(s) 126, 266, 379, 503, 707
HpyAV CCTTC 3 cut(s) 104, 316, 896
HpyCH4III ACNGT 3 cut(s) 153, 615, 671
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 1 cut(s) 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 55, 64, 99, 749
HpyF3I CTNAG 7 cut(s) 9, 181, 205, 380, 408, 859, 867
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 1 cut(s) 21
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 2 cut(s) 454, 1012
LmnI GCTCC 1 cut(s) 762
Lsp1109I GCAGC 3 cut(s) 45, 77, 925
LweI GCATC 2 cut(s) 236, 945
MaeI CTAG 1 cut(s) 587
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 3 cut(s) 340, 598, 993
MalI GATC 2 cut(s) 456, 1014
MboI GATC 2 cut(s) 454, 1012
MboII GAAGA 3 cut(s) 414, 574, 873
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 2 cut(s) 767, 860
MluCI AATT 6 cut(s) 192, 296, 534, 634, 688, 822
MnlI CCTC 9 cut(s) 64, 76, 122, 426, 466, 518, 583, 807, 856
MroI TCCGGA 1 cut(s) 502
MseI TTAA 3 cut(s) 164, 201, 933
MspI CCGG 2 cut(s) 121, 503
MspR9I CCNGG 4 cut(s) 521, 652, 980, 989
MvaI CCWGG 4 cut(s) 521, 652, 980, 989
MvnI CGCG 2 cut(s) 102, 440
MwoI GCNNNNNNNGC 5 cut(s) 35, 55, 64, 99, 749
NcoI CCATGG 1 cut(s) 17
NdeII GATC 2 cut(s) 454, 1012
NlaIII CATG 1 cut(s) 21
NlaIV GGNNCC 2 cut(s) 456, 764
NmuCI GTSAC 1 cut(s) 598
NspV TTCGAA 1 cut(s) 142
PaeR7I CTCGAG 1 cut(s) 68
PaqCI CACCTGC 1 cut(s) 541
PceI AGGCCT 1 cut(s) 86
PfeI GAWTC 3 cut(s) 139, 731, 983
PfoI TCCNGGA 1 cut(s) 650
PinAI ACCGGT 1 cut(s) 120
PkrI GCNGC 4 cut(s) 60, 92, 315, 940
Ppu21I YACGTR 1 cut(s) 364
Psp6I CCWGG 4 cut(s) 519, 650, 978, 987
PspFI CCCAGC 1 cut(s) 908
PspGI CCWGG 4 cut(s) 519, 650, 978, 987
PspN4I GGNNCC 2 cut(s) 456, 764
PspPI GGNCC 2 cut(s) 435, 500
PspXI VCTCGAGB 1 cut(s) 68
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 1 cut(s) 454
RsaI GTAC 4 cut(s) 327, 395, 564, 680
RsaNI GTAC 4 cut(s) 326, 394, 563, 679
SaqAI TTAA 3 cut(s) 164, 201, 933
SatI GCNGC 4 cut(s) 59, 91, 314, 939
Sau3AI GATC 2 cut(s) 454, 1012
Sau96I GGNCC 2 cut(s) 435, 500
ScaI AGTACT 1 cut(s) 564
ScrFI CCNGG 4 cut(s) 521, 652, 980, 989
SduI GDGCHC 2 cut(s) 767, 860
SfaNI GCATC 2 cut(s) 236, 945
SfcI CTRYAG 1 cut(s) 939
Sfr274I CTCGAG 1 cut(s) 68
SfuI TTCGAA 1 cut(s) 142
SinI GGWCC 2 cut(s) 435, 500
SlaI CTCGAG 1 cut(s) 68
SmlI CTYRAG 2 cut(s) 68, 264
SmoI CTYRAG 2 cut(s) 68, 264
SnaBI TACGTA 1 cut(s) 364
Sse9I AATT 6 cut(s) 192, 296, 534, 634, 688, 822
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 5 cut(s) 100, 313, 356, 438, 958
SspI AATATT 2 cut(s) 493, 930
SspMI CTAG 1 cut(s) 587
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 4 cut(s) 519, 650, 978, 987
StyI CCWWGG 1 cut(s) 17
TaaI ACNGT 3 cut(s) 153, 615, 671
TaiI ACGT 1 cut(s) 366
TaqI TCGA 5 cut(s) 69, 125, 142, 675, 953
TasI AATT 6 cut(s) 192, 296, 534, 634, 688, 822
TatI WGTACW 3 cut(s) 325, 562, 678
TauI GCSGC 1 cut(s) 316
TfiI GAWTC 3 cut(s) 139, 731, 983
Tru1I TTAA 3 cut(s) 164, 201, 933
Tru9I TTAA 3 cut(s) 164, 201, 933
TscAI CASTG 2 cut(s) 158, 233
TseFI GTSAC 1 cut(s) 598
TseI GCWGC 3 cut(s) 58, 90, 938
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 1 cut(s) 163
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 2 cut(s) 158, 233
VpaK11BI GGWCC 2 cut(s) 435, 500
XapI RAATTY 3 cut(s) 296, 634, 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XhoI CTCGAG 1 cut(s) 68
XspI CTAG 1 cut(s) 587
ZrmI AGTACT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.