FvH4_5g28680

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008616
Physical Location & Seq
Forward (+)
19852158 .. 19859655
7498 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28680.t2

Sequence Viewer

Length: 1011 bp
ATGGGAGGAAGAATGGAAGGTCTGGGCGCAAAAGGGAAGCTAGCGGCGGTGCTTTGGGTTGAGGGCTTCAGACAAGCCTGTTGCCTTCACCGTGTGCTTATTCTCTGCCACAGGTCCAGAAAGCTTCTGATACGCACGGGACAGTGTTTTCTGTTGAATGGTTTCATTTTCTTAGGAAGTATACTTATCCTTAACTCGTTCATCATCCCAGCTCTACACTGGATATTGCCTGCTATGTGTCCAGAGTTTAGTTCTCAACAGTTATGCTCATTTGCTGGCATTTGGGAATTTTATTCCATCTTACGTCATGGACTCGTACAGCTTTTTTATGTAACTTGGTTGTATCCATTATATGTATTCAGCTTTATCCTGAGCAACATCTGGTACAATGATATTGCCAAGTATGGATTTTATGCAATGGGGAGACCTGTGATTAATAAATTGGATCCTGTCAGACAAAGTGATGTGTTAACCTTCCAAAATGCAGCTGGCCTTGAAAGATCAACAGACCTAGGATGGGTCATGATTGGAATAGGAGAGCAGGTTTATTCAGTGCTTCTTTTGAGCTGTTTCTTCTTAGAGGTATATGTGACAGGTTTTATACCATATGTAGGCAAGGCATTAAATTTTCTGCTTCTTTCCTGGATGTATGCCTACTATTGTTTTGAGTACAAATGGAATTTCTCTGAAATCGGTCTGGACAAAAGGCTAGACTTCTTTGAATCTAACTGGGCATTTTTTGCTGGTTTTGGAAGCCCATGTGTTCTACCTCTTTTCTTTTTCTCACCTCTTGTGAGCTATGGGTGTATGGCTATACTCTTTCCGTTGTTTGTTTTGACAGCTACAGGCTCAGAAGCTGAGCAACTTATTTCTTCTCGAAAAACAAGCTGGGGTGACGAATGTTTCGGACGGCTTCAAATATTCAAGGCCGCAGATAGTTTGTCGATGCGGTTGTTGTCGTTATTTCCCTTGCAATCAAAGAGACATATCCAAGGACAGAAATATCTGTGA

Protein Analysis

337

Amino Acids

38.46

Weight (kDa)

8.7

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 24 - 271 2.9e-26 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 532
AccI GTMKAC 1 cut(s) 181
AciI CCGC 4 cut(s) 44, 47, 930, 949
AclWI GGATC 2 cut(s) 440, 453
AcsI RAATTY 3 cut(s) 287, 625, 679
AcuI CTGAAG 1 cut(s) 52
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 3 cut(s) 318, 386, 671
AfiI CCNNNNNNNGG 2 cut(s) 517, 611
AgsI TTSAA 5 cut(s) 157, 497, 722, 917, 925
AjnI CCWGG 1 cut(s) 641
Alw26I GTCTC 2 cut(s) 418, 976
AlwI GGATC 2 cut(s) 440, 453
AlwNI CAGNNNCTG 1 cut(s) 857
AoxI GGCC 2 cut(s) 490, 927
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 3 cut(s) 287, 625, 679
ArsI GACNNNNNNTTYG 2 cut(s) 887, 919
AseI ATTAAT 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 511
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 3 cut(s) 80, 777, 905
AsuNHI GCTAGC 1 cut(s) 40
AvaII GGWCC 1 cut(s) 114
AvrII CCTAGG 1 cut(s) 511
BamHI GGATCC 1 cut(s) 445
BbvI GCAGC 1 cut(s) 497
BccI CCATC 2 cut(s) 305, 510
BceAI ACGGC 1 cut(s) 926
BciT130I CCWGG 1 cut(s) 643
BciVI GTATCC 1 cut(s) 354
BcoDI GTCTC 2 cut(s) 418, 976
BfaI CTAG 3 cut(s) 41, 512, 710
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 532
BfuI GTATCC 1 cut(s) 354
BisI GCNGC 3 cut(s) 45, 486, 930
BlnI CCTAGG 1 cut(s) 511
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 3 cut(s) 46, 487, 931
Bme1390I CCNGG 1 cut(s) 643
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 1 cut(s) 643
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 1 cut(s) 936
BmtI GCTAGC 1 cut(s) 44
BmuI ACTGGG 1 cut(s) 739
Bpu10I CCTNAGC 1 cut(s) 371
Bpu1102I GCTNAGC 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 511, 991
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 611
Bse1I ACTGG 2 cut(s) 224, 734
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 1 cut(s) 643
BseDI CCNNGG 2 cut(s) 511, 991
BseGI GGATG 3 cut(s) 204, 521, 651
BseLI CCNNNNNNNGG 2 cut(s) 517, 611
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 3 cut(s) 362, 849, 864
BseNI ACTGG 2 cut(s) 224, 734
BseXI GCAGC 1 cut(s) 497
BseYI CCCAGC 2 cut(s) 208, 888
BshFI GGCC 2 cut(s) 492, 929
BslFI GGGAC 1 cut(s) 153
BslI CCNNNNNNNGG 2 cut(s) 517, 611
BsmAI GTCTC 2 cut(s) 418, 976
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 2 cut(s) 492, 929
Bso31I GGTCTC 1 cut(s) 418
Bsp143I GATC 2 cut(s) 445, 500
Bsp1720I GCTNAGC 1 cut(s) 858
BspACI CCGC 4 cut(s) 44, 47, 930, 949
BspANI GGCC 2 cut(s) 492, 929
BspCNI CTCAG 3 cut(s) 363, 850, 863
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 1 cut(s) 447
BspMI ACCTGC 1 cut(s) 532
BspOI GCTAGC 1 cut(s) 44
BspPI GGATC 2 cut(s) 440, 453
BspTNI GGTCTC 1 cut(s) 418
BsrDI GCAATG 1 cut(s) 423
BsrI ACTGG 2 cut(s) 224, 734
BssECI CCNNGG 2 cut(s) 511, 991
BssMI GATC 2 cut(s) 445, 500
BssNAI GTATAC 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 511, 991
Bst1107I GTATAC 1 cut(s) 182
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 3 cut(s) 92, 144, 261
BstAPI GCANNNNNTGC 1 cut(s) 740
BstC8I GCNNGC 4 cut(s) 42, 231, 277, 490
BstDEI CTNAG 5 cut(s) 172, 371, 577, 850, 858
BstF5I GGATG 3 cut(s) 204, 521, 651
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 2 cut(s) 448, 503
BstMAI GTCTC 2 cut(s) 418, 976
BstMBI GATC 2 cut(s) 445, 500
BstMWI GCNNNNNNNGC 1 cut(s) 740
BstNI CCWGG 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 641
BstSFI CTRYAG 1 cut(s) 843
BstV1I GCAGC 1 cut(s) 497
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BstZ17I GTATAC 1 cut(s) 182
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 2 cut(s) 492, 929
BtsCI GGATG 3 cut(s) 204, 521, 651
BtsIMutI CAGTG 3 cut(s) 149, 217, 558
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 4 cut(s) 42, 231, 277, 490
CaiI CAGNNNCTG 1 cut(s) 857
CciI TCATGA 1 cut(s) 522
CfoI GCGC 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 317, 385, 670
CviAII CATG 3 cut(s) 308, 523, 759
CviQI GTAC 3 cut(s) 317, 385, 670
DdeI CTNAG 5 cut(s) 172, 371, 577, 850, 858
DpnI GATC 2 cut(s) 447, 502
DpnII GATC 2 cut(s) 445, 500
DraIII CACNNNGTG 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 511, 991
Eco31I GGTCTC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 641
EcoT14I CCWWGG 2 cut(s) 511, 991
ErhI CCWWGG 2 cut(s) 511, 991
FaeI CATG 3 cut(s) 311, 526, 762
FaqI GGGAC 1 cut(s) 153
FatI CATG 3 cut(s) 307, 522, 758
FauNDI CATATG 1 cut(s) 607
FblI GTMKAC 1 cut(s) 181
Fnu4HI GCNGC 3 cut(s) 45, 486, 930
FokI GGATG 3 cut(s) 191, 528, 658
Fsp4HI GCNGC 3 cut(s) 45, 486, 930
FspBI CTAG 3 cut(s) 41, 512, 710
GlaI GCGC 1 cut(s) 28
GluI GCNGC 3 cut(s) 45, 486, 930
GsaI CCCAGC 2 cut(s) 212, 892
HaeIII GGCC 2 cut(s) 492, 929
HhaI GCGC 1 cut(s) 29
Hin1II CATG 3 cut(s) 311, 526, 762
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 2 cut(s) 312, 722
HpaI GTTAAC 1 cut(s) 471
HphI GGTGA 3 cut(s) 80, 777, 905
Hpy166II GTNNAC 2 cut(s) 182, 471
Hpy188I TCNGA 6 cut(s) 71, 129, 455, 688, 853, 908
Hpy188III TCNNGA 6 cut(s) 117, 242, 370, 523, 698, 876
Hpy8I GTNNAC 2 cut(s) 182, 471
HpyAV CCTTC 3 cut(s) 11, 95, 484
HpyCH4III ACNGT 3 cut(s) 92, 144, 261
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 3 cut(s) 416, 485, 973
HpyF10VI GCNNNNNNNGC 1 cut(s) 740
HpyF3I CTNAG 5 cut(s) 172, 371, 577, 850, 858
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 3 cut(s) 311, 526, 762
HspAI GCGC 1 cut(s) 27
KspAI GTTAAC 1 cut(s) 471
Kzo9I GATC 2 cut(s) 445, 500
Lsp1109I GCAGC 1 cut(s) 497
LweI GCATC 1 cut(s) 936
MaeI CTAG 3 cut(s) 41, 512, 710
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 3 cut(s) 331, 589, 893
MalI GATC 2 cut(s) 447, 502
MboI GATC 2 cut(s) 445, 500
MboII GAAGA 3 cut(s) 21, 565, 864
MflI RGATCY 1 cut(s) 445
MluCI AATT 4 cut(s) 287, 440, 625, 679
MlyI GAGTC 1 cut(s) 306
MnlI CCTC 4 cut(s) 55, 574, 780, 798
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 4 cut(s) 192, 435, 470, 623
MspA1I CMGCKG 1 cut(s) 488
MspR9I CCNGG 1 cut(s) 643
MvaI CCWGG 1 cut(s) 643
MwoI GCNNNNNNNGC 1 cut(s) 740
NdeI CATATG 1 cut(s) 607
NdeII GATC 2 cut(s) 445, 500
NheI GCTAGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 311, 526, 762
NlaIV GGNNCC 1 cut(s) 447
NmuCI GTSAC 2 cut(s) 589, 893
PagI TCATGA 1 cut(s) 522
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 1 cut(s) 722
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 3 cut(s) 46, 487, 931
PleI GAGTC 1 cut(s) 306
PpsI GAGTC 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 435
Psp6I CCWGG 1 cut(s) 641
PspFI CCCAGC 2 cut(s) 208, 888
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 1 cut(s) 447
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 1 cut(s) 445
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 3 cut(s) 318, 386, 671
RsaNI GTAC 3 cut(s) 317, 385, 670
SaqAI TTAA 4 cut(s) 192, 435, 470, 623
SatI GCNGC 3 cut(s) 45, 486, 930
Sau3AI GATC 2 cut(s) 445, 500
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 306
ScrFI CCNGG 1 cut(s) 643
SfaNI GCATC 1 cut(s) 936
SfcI CTRYAG 1 cut(s) 843
SinI GGWCC 1 cut(s) 114
Sse9I AATT 4 cut(s) 287, 440, 625, 679
SsiI CCGC 4 cut(s) 44, 47, 930, 949
SspI AATATT 1 cut(s) 921
SspMI CTAG 3 cut(s) 41, 512, 710
StyD4I CCNGG 1 cut(s) 641
StyI CCWWGG 2 cut(s) 511, 991
TaaI ACNGT 3 cut(s) 92, 144, 261
TaiI ACGT 1 cut(s) 307
TaqI TCGA 2 cut(s) 877, 944
TaqII GACCGA 1 cut(s) 683
TasI AATT 4 cut(s) 287, 440, 625, 679
TatI WGTACW 1 cut(s) 669
TauI GCSGC 2 cut(s) 47, 932
TfiI GAWTC 1 cut(s) 722
Tru1I TTAA 4 cut(s) 192, 435, 470, 623
Tru9I TTAA 4 cut(s) 192, 435, 470, 623
TscAI CASTG 3 cut(s) 149, 224, 558
TseFI GTSAC 2 cut(s) 589, 893
TseI GCWGC 1 cut(s) 485
Tsp45I GTSAC 2 cut(s) 589, 893
TspDTI ATGAA 2 cut(s) 154, 190
TspGWI ACGGA 1 cut(s) 813
TspRI CASTG 3 cut(s) 149, 224, 558
VpaK11BI GGWCC 1 cut(s) 114
VspI ATTAAT 1 cut(s) 435
XapI RAATTY 3 cut(s) 287, 625, 679
XcmI CCANNNNNNNNNTGG 2 cut(s) 216, 485
XmaJI CCTAGG 1 cut(s) 511
XmiI GTMKAC 1 cut(s) 181
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 3 cut(s) 41, 512, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.