RchiOBHm_Chr7g0220831

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008616
Physical Location & Seq
Reverse (-)
41715394 .. 41723644
8251 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19763

Sequence Viewer

Length: 999 bp
ATGGAAGCTCTGAGCGCAAAATCTAAGCTAGCCGCGGTGCTTTGGCTTGAGGGCTTCAGACAGGCCTGTTGCCTTCACCGTGTGCTGATTCTCTGCCACAGGTCCAGAAAGCTTCTGATACGCACAGGACAGTGTTTTCTGTTGAATGGTTTCATTTTCTTAGGAAGCATAATTATCCTTAACTCGTTCATCATCCCAGCTCTATACTGGATATTGCCTGATATGTGTCCAGAATCTAGTTCTCAAGAATTCTGCTCATTTGCTGTCATTTGGGAATTTTATTCCTTCTTACGTCATGGACTCATACAAATCTTTTATGTAACTTGGTTATACCCATTATATGTATTCAGCTTTATCCTGAGCAACATCTGGTACAATGATATTGCCAAGTATGGATTTTCTGCAATGGGGAGACCTGTGATTAACAAATTGGATCCCGTTAGGCAAAGTGATGCATCAACCTTACAAAATGCAGCTGGCATGGAAAGACCAACTGACCTAGGACGGGTCATGATTGGAATAGGAGAGCAGGTTTATTCAGTGCTTCTTTTGAGCTGTTTCTTCTTAGAGGTATATGTGACAGGATTTATACCATATGTGGGCAAGGCATTTAATTTTCTGCTTCTTTCCTGGATGTATGCCTACTACTGTTTTGAGTACAAATGGAATTTTTCTGAAATCGGTCTAGACAAAAGGCTGGACTTCTTTGAATCTAACTGGGCATTTTTTGCTGGTTTTGGAAGCCCATGCGTTGTACCTCTTTTTTTTTTCTCACCTCTTGTGAGCTATGGGTGTATGGCTATACTCTTTCCGTTGTTTGTTTTGACAGCTACAGGTTCAGAAGCTGAGCAACTTATTTCTTCTCGAAGAACAAGCTGGGGAGGCGCATGTTTCGGACGGCTTCAAATATTTAAGACTGCAGATAGTTTGTCGATGTGGTTGTTGTCGCTATTTCCCGTACAATCAAAGAGACGTCTTCAAGAACAGAAATATCTGTGA

Protein Analysis

332

Amino Acids

38.19

Weight (kDa)

8.58

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 24 - 271 2.3e-27 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 976
Acc36I ACCTGC 1 cut(s) 520
AccII CGCG 1 cut(s) 35
AciI CCGC 2 cut(s) 33, 35
AclWI GGATC 2 cut(s) 428, 441
AcsI RAATTY 3 cut(s) 248, 275, 667
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 1 cut(s) 973
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 4 cut(s) 374, 659, 756, 960
AfiI CCNNNNNNNGG 2 cut(s) 505, 599
AgsI TTSAA 4 cut(s) 145, 710, 905, 980
AjnI CCWGG 1 cut(s) 629
Alw26I GTCTC 2 cut(s) 406, 964
AlwI GGATC 2 cut(s) 428, 441
AlwNI CAGNNNCTG 1 cut(s) 845
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 3 cut(s) 248, 275, 667
Asp700I GAANNNNTTC 1 cut(s) 149
AspA2I CCTAGG 1 cut(s) 499
AspLEI GCGC 2 cut(s) 17, 887
AspS9I GGNCC 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 68, 765
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 102
AvrII CCTAGG 1 cut(s) 499
BamHI GGATCC 1 cut(s) 433
BbsI GAAGAC 1 cut(s) 968
BbvI GCAGC 1 cut(s) 485
BceAI ACGGC 1 cut(s) 914
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 2 cut(s) 406, 964
BfaI CTAG 4 cut(s) 29, 237, 500, 686
BfmI CTRYAG 2 cut(s) 831, 918
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 2 cut(s) 33, 474
BlnI CCTAGG 1 cut(s) 499
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 2 cut(s) 34, 475
Bme1390I CCNGG 1 cut(s) 631
Bme18I GGWCC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 631
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 2 cut(s) 442, 464
BmtI GCTAGC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 727
BpiI GAAGAC 1 cut(s) 968
Bpu10I CCTNAGC 1 cut(s) 359
Bpu1102I GCTNAGC 1 cut(s) 846
BpuEI CTTGAG 2 cut(s) 68, 228
BsaHI GRCGYC 1 cut(s) 973
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 2 cut(s) 33, 499
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 599
Bse1I ACTGG 2 cut(s) 212, 722
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 2 cut(s) 33, 499
BseGI GGATG 2 cut(s) 192, 639
BseLI CCNNNNNNNGG 2 cut(s) 505, 599
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 2 cut(s) 350, 837
BseNI ACTGG 2 cut(s) 212, 722
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 2 cut(s) 196, 876
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 505, 599
BsmAI GTCTC 2 cut(s) 406, 964
BsmBI CGTCTC 1 cut(s) 964
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 406
Bsp143I GATC 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 846
BspACI CCGC 2 cut(s) 33, 35
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 2 cut(s) 351, 838
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 435
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 1 cut(s) 520
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 2 cut(s) 428, 441
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 2 cut(s) 212, 722
BssECI CCNNGG 2 cut(s) 33, 499
BssMI GATC 1 cut(s) 433
BssNI GRCGYC 1 cut(s) 973
BssT1I CCWWGG 1 cut(s) 499
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 3 cut(s) 80, 132, 650
BstACI GRCGYC 1 cut(s) 973
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 2 cut(s) 30, 478
BstDEI CTNAG 6 cut(s) 11, 24, 160, 359, 565, 846
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 2 cut(s) 192, 639
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 2 cut(s) 17, 887
BstKTI GATC 1 cut(s) 436
BstMAI GTCTC 2 cut(s) 406, 964
BstMBI GATC 1 cut(s) 433
BstMWI GCNNNNNNNGC 3 cut(s) 14, 728, 882
BstNI CCWGG 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 891
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 2 cut(s) 831, 918
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 1 cut(s) 968
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 2 cut(s) 192, 639
BtsIMutI CAGTG 2 cut(s) 137, 546
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 2 cut(s) 30, 478
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 1 cut(s) 510
CfoI GCGC 2 cut(s) 17, 887
Cfr13I GGNCC 1 cut(s) 102
Cfr42I CCGCGG 1 cut(s) 36
Csp6I GTAC 4 cut(s) 373, 658, 755, 959
CviAII CATG 5 cut(s) 296, 481, 511, 747, 888
CviQI GTAC 4 cut(s) 373, 658, 755, 959
DdeI CTNAG 6 cut(s) 11, 24, 160, 359, 565, 846
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DraIII CACNNNGTG 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 499
Eco147I AGGCCT 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 40
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 1 cut(s) 499
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 499
Esp3I CGTCTC 1 cut(s) 964
FaeI CATG 5 cut(s) 299, 484, 514, 750, 891
FatI CATG 5 cut(s) 295, 480, 510, 746, 887
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 2 cut(s) 33, 474
FokI GGATG 2 cut(s) 179, 646
Fsp4HI GCNGC 2 cut(s) 33, 474
FspBI CTAG 4 cut(s) 29, 237, 500, 686
GlaI GCGC 2 cut(s) 16, 886
GluI GCNGC 2 cut(s) 33, 474
GsaI CCCAGC 2 cut(s) 200, 880
HaeIII GGCC 1 cut(s) 65
HhaI GCGC 2 cut(s) 17, 887
Hin1I GRCGYC 1 cut(s) 973
Hin1II CATG 5 cut(s) 299, 484, 514, 750, 891
Hin6I GCGC 2 cut(s) 15, 885
HinP1I GCGC 2 cut(s) 15, 885
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 4 cut(s) 88, 233, 300, 710
HphI GGTGA 2 cut(s) 68, 765
Hpy188I TCNGA 6 cut(s) 12, 59, 117, 676, 841, 896
Hpy188III TCNNGA 8 cut(s) 105, 230, 245, 358, 511, 686, 864, 980
HpyAV CCTTC 2 cut(s) 83, 295
HpyCH4III ACNGT 3 cut(s) 80, 132, 650
HpyCH4IV ACGT 2 cut(s) 292, 973
HpyCH4V TGCA 4 cut(s) 404, 455, 473, 920
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 728, 882
HpyF3I CTNAG 6 cut(s) 11, 24, 160, 359, 565, 846
HpySE526I ACGT 2 cut(s) 292, 973
Hsp92I GRCGYC 1 cut(s) 973
Hsp92II CATG 5 cut(s) 299, 484, 514, 750, 891
HspAI GCGC 2 cut(s) 15, 885
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 1 cut(s) 433
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 2 cut(s) 442, 464
MaeI CTAG 4 cut(s) 29, 237, 500, 686
MaeII ACGT 2 cut(s) 292, 973
MaeIII GTNAC 2 cut(s) 319, 577
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 4 cut(s) 553, 852, 879, 968
MflI RGATCY 1 cut(s) 433
MluCI AATT 6 cut(s) 171, 248, 275, 428, 613, 667
MlyI GAGTC 1 cut(s) 294
MnlI CCTC 5 cut(s) 43, 562, 768, 786, 875
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 4 cut(s) 180, 423, 612, 912
MspA1I CMGCKG 2 cut(s) 35, 476
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 14, 728, 882
NdeI CATATG 1 cut(s) 595
NdeII GATC 1 cut(s) 433
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 5 cut(s) 299, 484, 514, 750, 891
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 891
PagI TCATGA 1 cut(s) 510
PceI AGGCCT 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 3 cut(s) 88, 233, 710
PfoI TCCNGGA 1 cut(s) 629
PkrI GCNGC 2 cut(s) 34, 475
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
Psp6I CCWGG 1 cut(s) 629
PspFI CCCAGC 2 cut(s) 196, 876
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 1 cut(s) 102
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 476
RsaI GTAC 4 cut(s) 374, 659, 756, 960
RsaNI GTAC 4 cut(s) 373, 658, 755, 959
SacII CCGCGG 1 cut(s) 36
SaqAI TTAA 4 cut(s) 180, 423, 612, 912
SatI GCNGC 2 cut(s) 33, 474
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 1 cut(s) 102
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 631
SfaNI GCATC 2 cut(s) 442, 464
SfcI CTRYAG 2 cut(s) 831, 918
Sfr303I CCGCGG 1 cut(s) 36
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 1 cut(s) 102
SmlI CTYRAG 2 cut(s) 47, 243
SmoI CTYRAG 2 cut(s) 47, 243
Sse9I AATT 6 cut(s) 171, 248, 275, 428, 613, 667
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 2 cut(s) 33, 35
SspI AATATT 1 cut(s) 909
SspMI CTAG 4 cut(s) 29, 237, 500, 686
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 1 cut(s) 499
TaaI ACNGT 3 cut(s) 80, 132, 650
TaiI ACGT 2 cut(s) 295, 976
TaqI TCGA 2 cut(s) 865, 932
TaqII GACCGA 1 cut(s) 671
TasI AATT 6 cut(s) 171, 248, 275, 428, 613, 667
TatI WGTACW 1 cut(s) 657
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 88, 233, 710
Tru1I TTAA 4 cut(s) 180, 423, 612, 912
Tru9I TTAA 4 cut(s) 180, 423, 612, 912
TscAI CASTG 2 cut(s) 137, 546
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 142, 178
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 2 cut(s) 137, 546
VpaK11BI GGWCC 1 cut(s) 102
XapI RAATTY 3 cut(s) 248, 275, 667
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 1 cut(s) 891
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmaJI CCTAGG 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 4 cut(s) 29, 237, 500, 686
ZraI GACGTC 1 cut(s) 974
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.