RLG00000002199

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008616
Physical Location & Seq
Forward (+)
27095052 .. 27104435
9384 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002199

Sequence Viewer

Length: 1239 bp
ATGGAAGCTCTGAGCGCAAAATCAAAGCTAGCCGCGGTGCTTTGGCTTGAGGGCTTCAGACAGGCCTGTTGCCTTCACCGTGTGCTGATTCTCTGCCACAGGTCCAGAAAGCTTCTGATACGCACAGGACAGTGTTTTCTGTTGAATGGTTTCATTTTCCTAGGAAGCATAATTATCCTTAACTCGTTCATCATCCCAGCTCTATACTGGATATTGCCTGATATGTGTCCAGAATCTAGTTCTCAAGAATTCTGCTCATTTGCTGTCATTTGGGAATTTTACTCCTCCTTACGACATGGACTCATACAAGTCTTTTATGTAACTTGGTTATACCCATTATATGTATTCAGTTTTATCCTGAGCAACATCTGGTACAATGATATTGCCAAGTATGGATTTTCTGCAATGGGGAGACCTGTGATTAACAAATTGGATCCCGTCAGACAGAGTGATGCATCAACCTTACAAAATGCAGCTAGCATGGAAAGACCAACTGACCTAGGACGGGTCATGATTGGAATAGGAGAGCAGGTTTATTCAGTGCTTCTTTTGAGCTGTTTCTTCTTAGAGGTATATGTGACAGGATTTATACCATATGTGGGCAAGGCATTAAATTTTCTGCTTCTTTCCTGGATGTATGCCTACTACTGTTTTGAGTACAAATGGAATTTTTCTGAAATCGGTCTGGACAAAAGGCTGGACTTCTTTGAATCTAACTGGGCATTTTTTGCTGGTTTTGGAAGCCCATGTGTTCTACCTCTTTTCTTTTTCTCACCTCTTGTGAGCTATGGGTGTATGGCTATACTCTTTCCATTGTTTGTTTTGACAGCTACAGTTTGTCGATGCGGTTGTTGTCGCTATTTCCCTTACAATCAAAGAGACGTCTGCAAGAACAGAAATATCTGTGAGAAGGATGATAAATTCCTCCAAATCTGTTCATTATGTATGTTGGATGGTTCAAGAGAACAATTTGTGGATAAGTTTGAAGAACAGCTTGTTGTTTTCTTCTCTTATATTAGGCATGCCATTTTGGCGCTGTCGGCTTTGCCAGCCATGCTTCCTGTTAGCCTCGCCAGAGCTCGCTGGAAAGTTGACGTGTTGAGGACCAACCAGCCGGTGCTGTGCATCTCCGACATCTATGCACTCCCCGATCTTCACCTCTGGCGCGGCGTCGACTTCTTGCCCAGATTCCGGCTTCTGCTCCACTCTGCCGTCTCCGTTGCACATCACGGCGTTTGA

Protein Analysis

413

Amino Acids

47.43

Weight (kDa)

8.34

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 24 - 271 1.4e-27 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 885
Acc36I ACCTGC 1 cut(s) 520
AccI GTMKAC 1 cut(s) 1173
AccII CGCG 2 cut(s) 35, 1167
AciI CCGC 4 cut(s) 33, 35, 846, 1167
AclWI GGATC 2 cut(s) 428, 441
AcsI RAATTY 5 cut(s) 248, 275, 613, 667, 920
AcuI CTGAAG 1 cut(s) 40
AcyI GRCGYC 2 cut(s) 882, 1170
AdeI CACNNNGTG 1 cut(s) 82
AfaI GTAC 2 cut(s) 374, 659
AfiI CCNNNNNNNGG 3 cut(s) 505, 599, 1191
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 4 cut(s) 145, 710, 960, 986
AjiI CACGTC 1 cut(s) 1096
AjnI CCWGG 1 cut(s) 629
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 3 cut(s) 406, 873, 1219
AlwI GGATC 2 cut(s) 428, 441
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 5 cut(s) 248, 275, 613, 667, 920
Asp700I GAANNNNTTC 1 cut(s) 149
AspA2I CCTAGG 2 cut(s) 160, 499
AspLEI GCGC 3 cut(s) 17, 1036, 1167
AspS9I GGNCC 2 cut(s) 102, 1104
AsuHPI GGTGA 3 cut(s) 68, 765, 1148
AsuNHI GCTAGC 2 cut(s) 28, 476
AvaII GGWCC 2 cut(s) 102, 1104
AvrII CCTAGG 2 cut(s) 160, 499
BamHI GGATCC 1 cut(s) 433
BanII GRGCYC 1 cut(s) 1081
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 1 cut(s) 485
BccI CCATC 1 cut(s) 947
BceAI ACGGC 1 cut(s) 1196
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 3 cut(s) 406, 873, 1219
BfaI CTAG 5 cut(s) 29, 161, 237, 477, 500
BfmI CTRYAG 1 cut(s) 831
BfoI RGCGCY 1 cut(s) 1037
BfuAI ACCTGC 1 cut(s) 520
BglI GCCNNNNNGGC 1 cut(s) 1031
BisI GCNGC 3 cut(s) 33, 474, 1168
BlnI CCTAGG 2 cut(s) 160, 499
BlsI GCNGC 3 cut(s) 34, 475, 1169
Bme1390I CCNGG 1 cut(s) 631
Bme18I GGWCC 2 cut(s) 102, 1104
BmgBI CACGTC 1 cut(s) 1096
BmgT120I GGNCC 2 cut(s) 102, 1104
BmiI GGNNCC 1 cut(s) 435
BmrFI CCNGG 1 cut(s) 631
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 4 cut(s) 442, 464, 833, 1134
BmtI GCTAGC 2 cut(s) 32, 480
BmuI ACTGGG 1 cut(s) 727
Bpu10I CCTNAGC 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 68, 228
BsaHI GRCGYC 2 cut(s) 882, 1170
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 3 cut(s) 33, 160, 499
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 3 cut(s) 505, 599, 1191
Bse118I RCCGGY 1 cut(s) 1114
Bse1I ACTGG 2 cut(s) 212, 722
Bse3DI GCAATG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 3 cut(s) 33, 160, 499
BseGI GGATG 4 cut(s) 192, 639, 919, 958
BseLI CCNNNNNNNGG 3 cut(s) 505, 599, 1191
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 350
BseNI ACTGG 2 cut(s) 212, 722
BseRI GAGGAG 1 cut(s) 274
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 1 cut(s) 196
Bsh1236I CGCG 2 cut(s) 35, 1167
BshFI GGCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 1115, 1192
BslI CCNNNNNNNGG 3 cut(s) 505, 599, 1191
BsmAI GTCTC 3 cut(s) 406, 873, 1219
BsmBI CGTCTC 2 cut(s) 873, 1219
BsnI GGCC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp143I GATC 2 cut(s) 433, 1150
BspACI CCGC 4 cut(s) 33, 35, 846, 1167
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 351
BspFNI CGCG 2 cut(s) 35, 1167
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 435
BspMI ACCTGC 1 cut(s) 520
BspOI GCTAGC 2 cut(s) 32, 480
BspPI GGATC 2 cut(s) 428, 441
BspTNI GGTCTC 1 cut(s) 406
BsrDI GCAATG 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 1114
BsrI ACTGG 2 cut(s) 212, 722
BssAI RCCGGY 1 cut(s) 1114
BssECI CCNNGG 3 cut(s) 33, 160, 499
BssMI GATC 2 cut(s) 433, 1150
BssNI GRCGYC 2 cut(s) 882, 1170
BssT1I CCWWGG 2 cut(s) 160, 499
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 4 cut(s) 80, 132, 650, 835
BstACI GRCGYC 2 cut(s) 882, 1170
BstAPI GCANNNNNTGC 1 cut(s) 728
BstC8I GCNNGC 5 cut(s) 30, 478, 1023, 1050, 1081
BstDEI CTNAG 3 cut(s) 11, 359, 565
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 192, 639, 919, 958
BstFNI CGCG 2 cut(s) 35, 1167
BstH2I RGCGCY 1 cut(s) 1037
BstHHI GCGC 3 cut(s) 17, 1036, 1167
BstKTI GATC 2 cut(s) 436, 1153
BstMAI GTCTC 3 cut(s) 406, 873, 1219
BstMBI GATC 2 cut(s) 433, 1150
BstMWI GCNNNNNNNGC 6 cut(s) 14, 728, 1031, 1040, 1049, 1054
BstNI CCWGG 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 1025
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 2 cut(s) 35, 1167
BstV1I GCAGC 1 cut(s) 485
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuRI GGCC 1 cut(s) 65
BtgI CCRYGG 1 cut(s) 33
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 4 cut(s) 192, 639, 919, 958
BtsIMutI CAGTG 2 cut(s) 137, 546
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 5 cut(s) 30, 478, 1023, 1050, 1081
CciI TCATGA 1 cut(s) 510
CfoI GCGC 3 cut(s) 17, 1036, 1167
Cfr10I RCCGGY 1 cut(s) 1114
Cfr13I GGNCC 2 cut(s) 102, 1104
Cfr42I CCGCGG 1 cut(s) 36
CseI GACGC 1 cut(s) 1159
Csp6I GTAC 2 cut(s) 373, 658
CviAII CATG 6 cut(s) 296, 481, 511, 747, 1022, 1054
CviQI GTAC 2 cut(s) 373, 658
DdeI CTNAG 3 cut(s) 11, 359, 565
DpnI GATC 2 cut(s) 435, 1152
DpnII GATC 2 cut(s) 433, 1150
DraIII CACNNNGTG 1 cut(s) 82
Ecl136II GAGCTC 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 160, 499
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 1081
Eco31I GGTCTC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 102, 1104
Eco53kI GAGCTC 1 cut(s) 1079
Eco57I CTGAAG 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 1079
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 2 cut(s) 160, 499
EcoT22I ATGCAT 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 1081
ErhI CCWWGG 2 cut(s) 160, 499
Esp3I CGTCTC 2 cut(s) 873, 1219
FaeI CATG 6 cut(s) 299, 484, 514, 750, 1025, 1057
FalI AAGNNNNNCTT 2 cut(s) 978, 1010
FatI CATG 6 cut(s) 295, 480, 510, 746, 1021, 1053
FauNDI CATATG 1 cut(s) 595
FblI GTMKAC 1 cut(s) 1173
Fnu4HI GCNGC 3 cut(s) 33, 474, 1168
FokI GGATG 4 cut(s) 179, 646, 926, 965
FriOI GRGCYC 1 cut(s) 1081
Fsp4HI GCNGC 3 cut(s) 33, 474, 1168
FspBI CTAG 5 cut(s) 29, 161, 237, 477, 500
GlaI GCGC 3 cut(s) 16, 1035, 1166
GluI GCNGC 3 cut(s) 33, 474, 1168
GsaI CCCAGC 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 1037
HaeIII GGCC 1 cut(s) 65
HapII CCGG 2 cut(s) 1115, 1192
HgaI GACGC 1 cut(s) 1159
HhaI GCGC 3 cut(s) 17, 1036, 1167
Hin1I GRCGYC 2 cut(s) 882, 1170
Hin1II CATG 6 cut(s) 299, 484, 514, 750, 1025, 1057
Hin6I GCGC 3 cut(s) 15, 1034, 1165
HinP1I GCGC 3 cut(s) 15, 1034, 1165
HincII GTYRAC 2 cut(s) 1093, 1174
HindII GTYRAC 2 cut(s) 1093, 1174
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 5 cut(s) 88, 233, 300, 710, 1188
HpaII CCGG 2 cut(s) 1115, 1192
HphI GGTGA 3 cut(s) 68, 765, 1148
Hpy166II GTNNAC 2 cut(s) 1093, 1174
Hpy188I TCNGA 6 cut(s) 12, 59, 117, 443, 676, 1132
Hpy188III TCNNGA 7 cut(s) 105, 230, 245, 358, 511, 686, 960
Hpy8I GTNNAC 2 cut(s) 1093, 1174
Hpy99I CGWCG 1 cut(s) 1175
HpyAV CCTTC 2 cut(s) 83, 904
HpyCH4III ACNGT 4 cut(s) 80, 132, 650, 835
HpyCH4IV ACGT 2 cut(s) 882, 1095
HpyCH4V TGCA 7 cut(s) 404, 455, 473, 888, 1125, 1142, 1223
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 728, 1031, 1040, 1049, 1054
HpyF3I CTNAG 3 cut(s) 11, 359, 565
HpySE526I ACGT 2 cut(s) 882, 1095
Hsp92I GRCGYC 2 cut(s) 882, 1170
Hsp92II CATG 6 cut(s) 299, 484, 514, 750, 1025, 1057
HspAI GCGC 3 cut(s) 15, 1034, 1165
KspI CCGCGG 1 cut(s) 36
Kzo9I GATC 2 cut(s) 433, 1150
LmnI GCTCC 1 cut(s) 1206
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 4 cut(s) 442, 464, 833, 1134
MaeI CTAG 5 cut(s) 29, 161, 237, 477, 500
MaeII ACGT 2 cut(s) 882, 1095
MaeIII GTNAC 2 cut(s) 319, 577
MalI GATC 2 cut(s) 435, 1152
MboI GATC 2 cut(s) 433, 1150
MboII GAAGA 4 cut(s) 553, 997, 998, 1145
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 1081
MluCI AATT 8 cut(s) 171, 248, 275, 428, 613, 667, 920, 968
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 2 cut(s) 930, 1155
MnlI CCTC 9 cut(s) 43, 295, 562, 768, 786, 935, 1079, 1095, 1169
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 3 cut(s) 180, 423, 611
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 2 cut(s) 1115, 1192
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 2 cut(s) 35, 1167
MwoI GCNNNNNNNGC 6 cut(s) 14, 728, 1031, 1040, 1049, 1054
NdeI CATATG 1 cut(s) 595
NdeII GATC 2 cut(s) 433, 1150
NheI GCTAGC 2 cut(s) 28, 476
NlaIII CATG 6 cut(s) 299, 484, 514, 750, 1025, 1057
NlaIV GGNNCC 1 cut(s) 435
NmuCI GTSAC 1 cut(s) 577
NsiI ATGCAT 1 cut(s) 457
NspI RCATGY 1 cut(s) 1025
PaeI GCATGC 1 cut(s) 1025
PagI TCATGA 1 cut(s) 510
PceI AGGCCT 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 4 cut(s) 88, 233, 710, 1188
PfoI TCCNGGA 1 cut(s) 629
PkrI GCNGC 3 cut(s) 34, 475, 1169
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
Psp124BI GAGCTC 1 cut(s) 1081
Psp6I CCWGG 1 cut(s) 629
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 435
PspPI GGNCC 2 cut(s) 102, 1104
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 2 cut(s) 374, 659
RsaNI GTAC 2 cut(s) 373, 658
SacI GAGCTC 1 cut(s) 1081
SacII CCGCGG 1 cut(s) 36
SalI GTCGAC 1 cut(s) 1172
SaqAI TTAA 3 cut(s) 180, 423, 611
SatI GCNGC 3 cut(s) 33, 474, 1168
Sau3AI GATC 2 cut(s) 433, 1150
Sau96I GGNCC 2 cut(s) 102, 1104
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 631
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 4 cut(s) 442, 464, 833, 1134
SfcI CTRYAG 1 cut(s) 831
Sfr303I CCGCGG 1 cut(s) 36
SgrBI CCGCGG 1 cut(s) 36
SinI GGWCC 2 cut(s) 102, 1104
SmlI CTYRAG 2 cut(s) 47, 243
SmoI CTYRAG 2 cut(s) 47, 243
SphI GCATGC 1 cut(s) 1025
Sse9I AATT 8 cut(s) 171, 248, 275, 428, 613, 667, 920, 968
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 4 cut(s) 33, 35, 846, 1167
SspMI CTAG 5 cut(s) 29, 161, 237, 477, 500
SstI GAGCTC 1 cut(s) 1081
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 2 cut(s) 160, 499
TaaI ACNGT 4 cut(s) 80, 132, 650, 835
TaiI ACGT 2 cut(s) 885, 1098
TaqI TCGA 2 cut(s) 841, 1173
TaqII GACCGA 1 cut(s) 671
TasI AATT 8 cut(s) 171, 248, 275, 428, 613, 667, 920, 968
TatI WGTACW 1 cut(s) 657
TauI GCSGC 2 cut(s) 35, 1170
TfiI GAWTC 4 cut(s) 88, 233, 710, 1188
Tru1I TTAA 3 cut(s) 180, 423, 611
Tru9I TTAA 3 cut(s) 180, 423, 611
TscAI CASTG 2 cut(s) 137, 546
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 3 cut(s) 142, 178, 927
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 2 cut(s) 137, 546
VpaK11BI GGWCC 2 cut(s) 102, 1104
XapI RAATTY 5 cut(s) 248, 275, 613, 667, 920
XceI RCATGY 1 cut(s) 1025
XcmI CCANNNNNNNNNTGG 1 cut(s) 204
XmaJI CCTAGG 2 cut(s) 160, 499
XmiI GTMKAC 1 cut(s) 1173
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 5 cut(s) 29, 161, 237, 477, 500
ZraI GACGTC 1 cut(s) 883
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.