MD15G1432200.v1.1

Protein EI24 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008616
Physical Location & Seq
Reverse (-)
53193012 .. 53198599
5588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1432200.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGAAGCTACGAAGCCATGGATAAACGCTTTCAGAGCAAAATCAAAGCAAGCCACGCTGCTTTGGCTCGAGGGATTCCGAGAGGCCTGCTGCCTTCACCGTGTCCTCATCCTCTGCCACCGGTCGAGAAAGCTTATGATACGAACAGGACAGTGTTTTCTATTAAACGGCTTCATTTTCTTAGGAAGTATAATTATCCTTAACTCAGTCATCATCCCAACTCTACACTGGATATTACCTGATAGATGCCCAGAATATAGTTCTCAAGAACTCTGCTCATTTGTTGGTACATTGAAATTTTATTCCGTCTTGCGGAATGGACTCGTACAACTCTTTTATGTAACTTGGTTCTACCCACTGTACGTATTCAGCATTATCCTGAGCAACATCTGGTACAATGACATTGCTAAGTATGGATTTTCTGCAATGGGGAGATCCGCGCCAACCACATTGGATCCTGTCAGACAAAATGATGTTGGTTTGGAAAGACCTGCTGGCGTAGAAGGGGTCATGATTGGTATAGGAGAGCAGGTGTATTCATTACTTCTTTTGAACTGTTTCTTCCTAGAGGTATATGTGACTGGATATATACCATATGTGGGGAAGGCAGTAAATTTTCTGCTTCTGTCCTGGATGTATGCGTATTACTGTTTCGAGTACAAATGGAATTTTACTGAAGTTCGTCTTGAAAAAAGGCTGGATTTCTTTGAATCTAATTGGGCATTTTTTGCTGGTTTTGGGAGCCCTTGCGTTCTGCCTGTACTCTTTTTCTCACCTCTTGTGAGCTATGGATTTATGGCAATACTCTTTCCATTGTTTGTTTTGACAGCAACAGGTTCAGAGGCTGAGCAAGTTATTTCTTCTCAAAGGACAAAATGGGGAGGTGCTGGGTTGCGAAAGCTTCCAATATTTAATGCTGCAGATAAATTATCGATGCGGGTTTTGTCTCTGTTTCCCCTAGAATCTCAGGAACTGCTTCAAAAAAAGAAAGACCGCTAA

Protein Analysis

333

Amino Acids

38.13

Weight (kDa)

8.97

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EI24 PF07264 31 - 271 3.2e-28 Sulfate transporter CysZ/Etoposide-induced protein 2.4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 2 cut(s) 499, 520
AccII CGCG 1 cut(s) 440
AciI CCGC 4 cut(s) 313, 438, 937, 994
AclWI GGATC 3 cut(s) 429, 449, 462
AcsI RAATTY 3 cut(s) 296, 613, 667
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 6 cut(s) 289, 327, 362, 395, 659, 762
AfiI CCNNNNNNNGG 2 cut(s) 312, 599
AgeI ACCGGT 1 cut(s) 120
AgsI TTSAA 5 cut(s) 295, 553, 689, 710, 980
AjnI CCWGG 1 cut(s) 629
AluBI AGCT 4 cut(s) 8, 133, 786, 901
AluI AGCT 4 cut(s) 8, 133, 786, 901
Alw26I GTCTC 1 cut(s) 951
AlwI GGATC 3 cut(s) 429, 449, 462
AlwNI CAGNNNCTG 2 cut(s) 845, 973
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 3 cut(s) 58, 90, 917
ApoI RAATTY 3 cut(s) 296, 613, 667
AsiGI ACCGGT 1 cut(s) 120
Asp700I GAANNNNTTC 2 cut(s) 557, 975
AspLEI GCGC 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 89, 765
AvaI CYCGRG 1 cut(s) 68
BamHI GGATCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 746
BbvI GCAGC 3 cut(s) 45, 77, 904
BceAI ACGGC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 1 cut(s) 951
BfaI CTAG 2 cut(s) 566, 959
BfmI CTRYAG 1 cut(s) 918
BfuAI ACCTGC 2 cut(s) 499, 520
BisI GCNGC 3 cut(s) 59, 91, 918
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 3 cut(s) 60, 92, 919
Bme1390I CCNGG 1 cut(s) 631
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 2 cut(s) 456, 743
BmrFI CCNGG 1 cut(s) 631
BmsI GCATC 2 cut(s) 236, 924
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 846
BpuEI CTTGAG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 932
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 1 cut(s) 17
BsaWI WCCGGW 1 cut(s) 120
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 2 cut(s) 312, 599
Bse118I RCCGGY 1 cut(s) 120
Bse1I ACTGG 2 cut(s) 233, 586
Bse3DI GCAATG 2 cut(s) 402, 432
BseBI CCWGG 1 cut(s) 631
BseCI ATCGAT 1 cut(s) 932
BseDI CCNNGG 1 cut(s) 17
BseGI GGATG 3 cut(s) 108, 213, 639
BseLI CCNNNNNNNGG 2 cut(s) 312, 599
BseMI GCAATG 2 cut(s) 402, 432
BseMII CTCAG 4 cut(s) 219, 371, 837, 980
BseNI ACTGG 2 cut(s) 233, 586
BseXI GCAGC 3 cut(s) 45, 77, 904
BseYI CCCAGC 1 cut(s) 887
Bsh1236I CGCG 1 cut(s) 440
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 1 cut(s) 86
BshTI ACCGGT 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 121
BslI CCNNNNNNNGG 2 cut(s) 312, 599
BsmAI GTCTC 1 cut(s) 951
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 746
Bsp143I GATC 2 cut(s) 434, 454
Bsp1720I GCTNAGC 1 cut(s) 846
Bsp19I CCATGG 1 cut(s) 17
BspACI CCGC 4 cut(s) 313, 438, 937, 994
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 4 cut(s) 218, 372, 838, 979
BspDI ATCGAT 1 cut(s) 932
BspFNI CGCG 1 cut(s) 440
BspHI TCATGA 1 cut(s) 510
BspLI GGNNCC 2 cut(s) 456, 743
BspMAI CTGCAG 1 cut(s) 922
BspMI ACCTGC 2 cut(s) 499, 520
BspPI GGATC 3 cut(s) 429, 449, 462
BsrDI GCAATG 2 cut(s) 402, 432
BsrFI RCCGGY 1 cut(s) 120
BsrI ACTGG 2 cut(s) 233, 586
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 17
BssMI GATC 2 cut(s) 434, 454
BssT1I CCWWGG 1 cut(s) 17
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 5 cut(s) 101, 153, 360, 557, 650
BstAPI GCANNNNNTGC 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 51, 88, 496
BstDEI CTNAG 6 cut(s) 181, 205, 380, 408, 846, 966
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 108, 213, 639
BstFNI CGCG 1 cut(s) 440
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 437, 457
BstMAI GTCTC 1 cut(s) 951
BstMBI GATC 2 cut(s) 434, 454
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 4 cut(s) 35, 55, 64, 728
BstNI CCWGG 1 cut(s) 631
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 1 cut(s) 918
BstSNI TACGTA 1 cut(s) 364
BstUI CGCG 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 45, 77, 904
BstX2I RGATCY 2 cut(s) 434, 454
BstYI RGATCY 2 cut(s) 434, 454
Bsu15I ATCGAT 1 cut(s) 932
BsuRI GGCC 1 cut(s) 86
BsuTUI ATCGAT 1 cut(s) 932
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 3 cut(s) 108, 213, 639
BtsIMutI CAGTG 3 cut(s) 158, 226, 356
BveI ACCTGC 2 cut(s) 499, 520
Cac8I GCNNGC 3 cut(s) 51, 88, 496
CaiI CAGNNNCTG 2 cut(s) 845, 973
CciI TCATGA 1 cut(s) 510
CfoI GCGC 1 cut(s) 442
Cfr10I RCCGGY 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 932
Csp6I GTAC 6 cut(s) 288, 326, 361, 394, 658, 761
CspAI ACCGGT 1 cut(s) 120
CviAII CATG 2 cut(s) 18, 511
CviQI GTAC 6 cut(s) 288, 326, 361, 394, 658, 761
DdeI CTNAG 6 cut(s) 181, 205, 380, 408, 846, 966
DpnI GATC 2 cut(s) 436, 456
DpnII GATC 2 cut(s) 434, 454
Eco105I TACGTA 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 17
Eco147I AGGCCT 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 1 cut(s) 17
EcoT38I GRGCYC 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 17
FaeI CATG 2 cut(s) 21, 514
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FatI CATG 2 cut(s) 17, 510
FauI CCCGC 1 cut(s) 930
FauNDI CATATG 1 cut(s) 595
Fnu4HI GCNGC 3 cut(s) 59, 91, 918
FokI GGATG 3 cut(s) 95, 200, 646
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 3 cut(s) 59, 91, 918
FspBI CTAG 2 cut(s) 566, 959
GlaI GCGC 1 cut(s) 441
GluI GCNGC 3 cut(s) 59, 91, 918
GsaI CCCAGC 1 cut(s) 891
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 121
HhaI GCGC 1 cut(s) 442
Hin1II CATG 2 cut(s) 21, 514
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 2 cut(s) 131, 899
HinfI GANTC 4 cut(s) 75, 321, 710, 962
HpaII CCGG 1 cut(s) 121
HphI GGTGA 2 cut(s) 89, 765
Hpy188I TCNGA 4 cut(s) 35, 80, 464, 841
Hpy188III TCNNGA 6 cut(s) 126, 266, 379, 511, 686, 968
HpyAV CCTTC 3 cut(s) 104, 497, 598
HpyCH4III ACNGT 5 cut(s) 101, 153, 360, 557, 650
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 2 cut(s) 425, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 55, 64, 728
HpyF3I CTNAG 6 cut(s) 181, 205, 380, 408, 846, 966
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 2 cut(s) 21, 514
HspAI GCGC 1 cut(s) 440
Kzo9I GATC 2 cut(s) 434, 454
LmnI GCTCC 1 cut(s) 741
Lsp1109I GCAGC 3 cut(s) 45, 77, 904
LweI GCATC 2 cut(s) 236, 924
MaeI CTAG 2 cut(s) 566, 959
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 2 cut(s) 340, 577
MalI GATC 2 cut(s) 436, 456
MboI GATC 2 cut(s) 434, 454
MboII GAAGA 2 cut(s) 553, 852
MflI RGATCY 2 cut(s) 434, 454
MhlI GDGCHC 1 cut(s) 746
MluCI AATT 6 cut(s) 192, 296, 613, 667, 715, 926
MlyI GAGTC 1 cut(s) 315
MnlI CCTC 8 cut(s) 64, 76, 116, 122, 562, 786, 835, 875
MroXI GAANNNNTTC 2 cut(s) 557, 975
MseI TTAA 3 cut(s) 164, 201, 912
MspI CCGG 1 cut(s) 121
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 440
MwoI GCNNNNNNNGC 4 cut(s) 35, 55, 64, 728
NcoI CCATGG 1 cut(s) 17
NdeI CATATG 1 cut(s) 595
NdeII GATC 2 cut(s) 434, 454
NlaIII CATG 2 cut(s) 21, 514
NlaIV GGNNCC 2 cut(s) 456, 743
NmuCI GTSAC 1 cut(s) 577
PaeR7I CTCGAG 1 cut(s) 68
PagI TCATGA 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 520
PceI AGGCCT 1 cut(s) 86
PdmI GAANNNNTTC 2 cut(s) 557, 975
PfeI GAWTC 3 cut(s) 75, 710, 962
PfoI TCCNGGA 1 cut(s) 629
PinAI ACCGGT 1 cut(s) 120
PkrI GCNGC 3 cut(s) 60, 92, 919
PleI GAGTC 1 cut(s) 315
PpsI GAGTC 1 cut(s) 315
Ppu21I YACGTR 1 cut(s) 364
Psp6I CCWGG 1 cut(s) 629
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 456, 743
PspXI VCTCGAGB 1 cut(s) 68
PstI CTGCAG 1 cut(s) 922
PstNI CAGNNNCTG 2 cut(s) 845, 973
PsuI RGATCY 2 cut(s) 434, 454
RsaI GTAC 6 cut(s) 289, 327, 362, 395, 659, 762
RsaNI GTAC 6 cut(s) 288, 326, 361, 394, 658, 761
SaqAI TTAA 3 cut(s) 164, 201, 912
SatI GCNGC 3 cut(s) 59, 91, 918
Sau3AI GATC 2 cut(s) 434, 454
SchI GAGTC 1 cut(s) 315
ScrFI CCNGG 1 cut(s) 631
SduI GDGCHC 1 cut(s) 746
SfaNI GCATC 2 cut(s) 236, 924
SfcI CTRYAG 1 cut(s) 918
Sfr274I CTCGAG 1 cut(s) 68
SlaI CTCGAG 1 cut(s) 68
SmlI CTYRAG 2 cut(s) 68, 264
SmoI CTYRAG 2 cut(s) 68, 264
SnaBI TACGTA 1 cut(s) 364
Sse9I AATT 6 cut(s) 192, 296, 613, 667, 715, 926
SseBI AGGCCT 1 cut(s) 86
SsiI CCGC 4 cut(s) 313, 438, 937, 994
SspI AATATT 1 cut(s) 909
SspMI CTAG 2 cut(s) 566, 959
StuI AGGCCT 1 cut(s) 86
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 1 cut(s) 17
TaaI ACNGT 5 cut(s) 101, 153, 360, 557, 650
TaiI ACGT 1 cut(s) 366
TaqI TCGA 4 cut(s) 69, 125, 654, 932
TasI AATT 6 cut(s) 192, 296, 613, 667, 715, 926
TatI WGTACW 2 cut(s) 657, 760
TfiI GAWTC 3 cut(s) 75, 710, 962
Tru1I TTAA 3 cut(s) 164, 201, 912
Tru9I TTAA 3 cut(s) 164, 201, 912
TscAI CASTG 3 cut(s) 158, 233, 363
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 3 cut(s) 58, 90, 917
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 2 cut(s) 163, 528
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 3 cut(s) 158, 233, 363
XapI RAATTY 3 cut(s) 296, 613, 667
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XhoI CTCGAG 1 cut(s) 68
XmnI GAANNNNTTC 2 cut(s) 557, 975
XspI CTAG 2 cut(s) 566, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.