AT4G10000

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006188
Physical Location & Seq
Reverse (-)
6260302 .. 6263181
2880 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10000.2

Sequence Viewer

Length: 1002 bp
ATGTCAATTTCGGTGGTTGATAGCAGTCTCTGCCATCCGACTGCGTCGTTTCCGTCATCCATGGTTCATTCTAATCGATTCTCGAAGAGAATATCAGGGAACGGGAATTGGGTTCGCGAAAGGAGAAGACTTTATGTGAAATCGAGTAACTCAGAAGGTAAAAAGGAGGAAGCAGCACAGAAATCGAGCAGTAATAATACATCTAGCTTCTTGTCTTTTCTCTGTCCATTGCTTAAAGTTTTCTCTGGAGGTGATCCTTCTCAACAGCGGAATCATGCTCTCGAGGTTGCAACTTCTTCATTGGCATCTGTTGCAAGACTTCCATGGGGATCTCGAGTATCAACTGGGAGTATAGATAACCAGGATGTCTCTTCTAATCCGCCTTTGCGATTGCAGCTTTTTGAATTTGAGGCATGTCCCTTTTGTCGGAGAGTGCGGGAAGCCATGACTGAGCTAGACCTTTCAGTAGAAGTTTATCCTTGTCCTAAAGGATCTATAAGACACAGAGAACTGGTTAGAAGATCTGGCGGAAAAGAGATGTTTCCATTTCTCGTCGACCCGAATACTGAAACTTTAATGTATGAGAGTGGTGACATCGTAAAGTACCTATTTAAACAATATGGAAATGGAAGAGGCCCTTCCACAGGACTTCTGGAAAGCACCTTGTTCACTGGATGGATGCCAACTCTTCTCCGAGCAGGTAGAGGCATGTCATTGTGGGATAAGGCTTCAACAGATCTGCCACCTAAAATGCTCGAGCTCTTCTCTTACGAAAACAACCCGTATTCACGGTTGGTGCGTGAAGCACTGTGTGAGCTGGAGCTTCCCTATGTATTACACAACATTGGAGAAGGGTCCACACGGATGAAATCACTTCTAAATGCTTCAGGATCCAATAAGGTTCCATTCTTGGTTGATCCAAACACCGGTGTCCAATTAGGTGATTACGAGAAGATTCTGGCTTACCTCTTCAAGACATATTCTTCAGCAGCATTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

333

Amino Acids

37.06

Weight (kDa)

8.95

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 133 - 209 6.5e-20 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 138 - 203 7e-08 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 254 - 328 5.6e-11 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 261 - 323 9.1e-06 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 689
AccI GTMKAC 1 cut(s) 555
AccII CGCG 1 cut(s) 117
AciI CCGC 4 cut(s) 268, 380, 436, 528
AclWI GGATC 6 cut(s) 248, 337, 499, 885, 898, 911
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 870, 969
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 3 cut(s) 426, 644, 926
AgeI ACCGGT 1 cut(s) 926
AgsI TTSAA 3 cut(s) 404, 732, 973
AjnI CCWGG 1 cut(s) 360
AluBI AGCT 6 cut(s) 207, 397, 454, 760, 817, 823
AluI AGCT 6 cut(s) 207, 397, 454, 760, 817, 823
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 2 cut(s) 32, 373
AlwI GGATC 6 cut(s) 248, 337, 499, 885, 898, 911
AlwNI CAGNNNCTG 1 cut(s) 30
Ama87I CYCGRG 3 cut(s) 281, 333, 755
AoxI GGCC 1 cut(s) 634
ApeKI GCWGC 3 cut(s) 173, 394, 989
ApoI RAATTY 1 cut(s) 404
AsiGI ACCGGT 1 cut(s) 926
AspS9I GGNCC 2 cut(s) 635, 855
AsuHPI GGTGA 3 cut(s) 263, 602, 953
AvaI CYCGRG 3 cut(s) 281, 333, 755
AvaII GGWCC 1 cut(s) 855
BamHI GGATCC 1 cut(s) 890
BanII GRGCYC 1 cut(s) 762
BbsI GAAGAC 1 cut(s) 133
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 2 cut(s) 185, 406
BccI CCATC 2 cut(s) 42, 669
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BciT130I CCWGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 32, 373
BfaI CTAG 2 cut(s) 204, 455
BfuAI ACCTGC 1 cut(s) 689
BglII AGATCT 2 cut(s) 521, 736
BisI GCNGC 3 cut(s) 174, 395, 990
BlsI GCNGC 3 cut(s) 175, 396, 991
Bme1390I CCNGG 1 cut(s) 362
Bme18I GGWCC 1 cut(s) 855
BmeT110I CYCGRG 3 cut(s) 281, 333, 755
BmgT120I GGNCC 2 cut(s) 635, 855
BmiI GGNNCC 3 cut(s) 856, 892, 903
BmrFI CCNGG 1 cut(s) 362
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 314, 669
BmuI ACTGGG 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 749, 781
BpmI CTGGAG 2 cut(s) 267, 839
Bsa29I ATCGAT 1 cut(s) 76
BsaJI CCNNGG 2 cut(s) 60, 323
BsaWI WCCGGW 1 cut(s) 926
Bsc4I CCNNNNNNNGG 3 cut(s) 426, 644, 926
Bse118I RCCGGY 1 cut(s) 926
Bse1I ACTGG 3 cut(s) 349, 516, 676
Bse3DI GCAATG 1 cut(s) 227
BseBI CCWGG 1 cut(s) 362
BseCI ATCGAT 1 cut(s) 76
BseDI CCNNGG 2 cut(s) 60, 323
BseGI GGATG 6 cut(s) 34, 56, 370, 680, 684, 870
BseLI CCNNNNNNNGG 3 cut(s) 426, 644, 926
BseMI GCAATG 1 cut(s) 227
BseMII CTCAG 2 cut(s) 165, 441
BseNI ACTGG 3 cut(s) 349, 516, 676
BseXI GCAGC 2 cut(s) 185, 406
Bsh1236I CGCG 1 cut(s) 117
BshFI GGCC 1 cut(s) 636
BshTI ACCGGT 1 cut(s) 926
BshVI ATCGAT 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 3 cut(s) 281, 333, 755
BsiSI CCGG 1 cut(s) 927
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 3 cut(s) 426, 644, 926
BsmAI GTCTC 2 cut(s) 32, 373
BsmFI GGGAC 1 cut(s) 402
BsnI GGCC 1 cut(s) 636
BsoBI CYCGRG 3 cut(s) 281, 333, 755
Bsp1286I GDGCHC 1 cut(s) 762
Bsp143I GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
Bsp19I CCATGG 2 cut(s) 60, 323
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 4 cut(s) 268, 380, 436, 528
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 2 cut(s) 164, 442
BspDI ATCGAT 1 cut(s) 76
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 3 cut(s) 856, 892, 903
BspMI ACCTGC 1 cut(s) 689
BspPI GGATC 6 cut(s) 248, 337, 499, 885, 898, 911
BspQI GCTCTTC 1 cut(s) 767
BsrDI GCAATG 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 926
BsrI ACTGG 3 cut(s) 349, 516, 676
BssAI RCCGGY 1 cut(s) 926
BssECI CCNNGG 2 cut(s) 60, 323
BssMI GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
BssT1I CCWWGG 2 cut(s) 60, 323
Bst2UI CCWGG 1 cut(s) 362
Bst4CI ACNGT 2 cut(s) 792, 810
Bst6I CTCTTC 6 cut(s) 80, 376, 625, 693, 767, 974
BstAPI GCANNNNNTGC 3 cut(s) 30, 311, 995
BstDEI CTNAG 2 cut(s) 151, 450
BstDSI CCRYGG 2 cut(s) 60, 323
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 6 cut(s) 34, 56, 370, 680, 684, 870
BstFNI CGCG 1 cut(s) 117
BstKTI GATC 7 cut(s) 256, 332, 494, 524, 739, 893, 919
BstMAI GTCTC 2 cut(s) 32, 373
BstMBI GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
BstMWI GCNNNNNNNGC 4 cut(s) 30, 311, 394, 995
BstNI CCWGG 1 cut(s) 362
BstNSI RCATGY 2 cut(s) 417, 712
BstSCI CCNGG 1 cut(s) 360
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 185, 406
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 5 cut(s) 329, 491, 521, 736, 890
BstYI RGATCY 5 cut(s) 329, 491, 521, 736, 890
Bsu15I ATCGAT 1 cut(s) 76
BsuRI GGCC 1 cut(s) 636
BsuTUI ATCGAT 1 cut(s) 76
BtgI CCRYGG 2 cut(s) 60, 323
BtsCI GGATG 6 cut(s) 34, 56, 370, 680, 684, 870
BtsIMutI CAGTG 2 cut(s) 669, 806
BtuMI TCGCGA 1 cut(s) 117
BveI ACCTGC 1 cut(s) 689
CaiI CAGNNNCTG 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 926
Cfr13I GGNCC 2 cut(s) 635, 855
ClaI ATCGAT 1 cut(s) 76
CseI GACGC 1 cut(s) 33
Csp6I GTAC 1 cut(s) 604
CspAI ACCGGT 1 cut(s) 926
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 6 cut(s) 61, 275, 324, 414, 445, 709
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 2 cut(s) 151, 450
DpnI GATC 7 cut(s) 255, 331, 493, 523, 738, 892, 918
DpnII GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
DraI TTTAAA 1 cut(s) 613
DraIII CACNNNGTG 1 cut(s) 812
Eam1104I CTCTTC 6 cut(s) 80, 376, 625, 693, 767, 974
EarI CTCTTC 6 cut(s) 80, 376, 625, 693, 767, 974
EciI GGCGGA 2 cut(s) 369, 543
Ecl136II GAGCTC 1 cut(s) 760
Eco130I CCWWGG 2 cut(s) 60, 323
Eco24I GRGCYC 1 cut(s) 762
Eco47I GGWCC 1 cut(s) 855
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 870, 969
Eco88I CYCGRG 3 cut(s) 281, 333, 755
EcoICRI GAGCTC 1 cut(s) 760
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 635
EcoRII CCWGG 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 60, 323
EcoT38I GRGCYC 1 cut(s) 762
ErhI CCWWGG 2 cut(s) 60, 323
FaeI CATG 6 cut(s) 64, 278, 327, 417, 448, 712
FalI AAGNNNNNCTT 2 cut(s) 622, 654
FaqI GGGAC 1 cut(s) 402
FatI CATG 6 cut(s) 60, 274, 323, 413, 444, 708
FauI CCCGC 1 cut(s) 429
FblI GTMKAC 1 cut(s) 555
Fnu4HI GCNGC 3 cut(s) 174, 395, 990
FokI GGATG 6 cut(s) 21, 43, 377, 687, 691, 877
FriOI GRGCYC 1 cut(s) 762
Fsp4HI GCNGC 3 cut(s) 174, 395, 990
FspBI CTAG 2 cut(s) 204, 455
GluI GCNGC 3 cut(s) 174, 395, 990
GsuI CTGGAG 2 cut(s) 267, 839
HaeIII GGCC 1 cut(s) 636
HapII CCGG 1 cut(s) 927
HgaI GACGC 1 cut(s) 33
Hin1II CATG 6 cut(s) 64, 278, 327, 417, 448, 712
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 3 cut(s) 78, 271, 955
HpaII CCGG 1 cut(s) 927
HphI GGTGA 3 cut(s) 263, 602, 953
Hpy166II GTNNAC 3 cut(s) 556, 669, 858
Hpy188I TCNGA 4 cut(s) 39, 154, 429, 695
Hpy188III TCNNGA 8 cut(s) 82, 116, 246, 281, 333, 653, 888, 973
Hpy8I GTNNAC 3 cut(s) 556, 669, 858
Hpy99I CGWCG 2 cut(s) 49, 557
HpyAV CCTTC 4 cut(s) 149, 267, 648, 845
HpyCH4III ACNGT 2 cut(s) 792, 810
HpyCH4V TGCA 4 cut(s) 290, 314, 394, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 311, 394, 995
HpyF3I CTNAG 2 cut(s) 151, 450
Hsp92II CATG 6 cut(s) 64, 278, 327, 417, 448, 712
Kzo9I GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
LguI GCTCTTC 1 cut(s) 767
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 2 cut(s) 185, 406
LweI GCATC 2 cut(s) 314, 669
MaeI CTAG 2 cut(s) 204, 455
MaeIII GTNAC 2 cut(s) 146, 590
MalI GATC 7 cut(s) 255, 331, 493, 523, 738, 892, 918
MboI GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
MflI RGATCY 5 cut(s) 329, 491, 521, 736, 890
MhlI GDGCHC 1 cut(s) 762
MluCI AATT 4 cut(s) 6, 106, 404, 935
MmeI TCCRAC 2 cut(s) 62, 407
MnlI CCTC 7 cut(s) 160, 242, 277, 403, 626, 698, 977
MseI TTAA 3 cut(s) 234, 575, 612
MslI CAYNNNNRTG 1 cut(s) 863
MspA1I CMGCKG 1 cut(s) 268
MspI CCGG 1 cut(s) 927
MspR9I CCNGG 1 cut(s) 362
MvaI CCWGG 1 cut(s) 362
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 4 cut(s) 30, 311, 394, 995
NcoI CCATGG 2 cut(s) 60, 323
NdeII GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
NlaIII CATG 6 cut(s) 64, 278, 327, 417, 448, 712
NlaIV GGNNCC 3 cut(s) 856, 892, 903
NmuCI GTSAC 1 cut(s) 590
NruI TCGCGA 1 cut(s) 117
NspI RCATGY 2 cut(s) 417, 712
PaeR7I CTCGAG 3 cut(s) 281, 333, 755
PciSI GCTCTTC 1 cut(s) 767
PcsI WCGNNNNNNNCGW 1 cut(s) 796
PfeI GAWTC 3 cut(s) 78, 271, 955
PflFI GACNNNGTC 1 cut(s) 43
PinAI ACCGGT 1 cut(s) 926
PkrI GCNGC 3 cut(s) 175, 396, 991
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 360
PspGI CCWGG 1 cut(s) 360
PspN4I GGNNCC 3 cut(s) 856, 892, 903
PspPI GGNCC 2 cut(s) 635, 855
PspXI VCTCGAGB 1 cut(s) 755
PstNI CAGNNNCTG 1 cut(s) 30
PsuI RGATCY 5 cut(s) 329, 491, 521, 736, 890
PsyI GACNNNGTC 1 cut(s) 43
RruI TCGCGA 1 cut(s) 117
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 1 cut(s) 863
SacI GAGCTC 1 cut(s) 762
SalI GTCGAC 1 cut(s) 554
SapI GCTCTTC 1 cut(s) 767
SaqAI TTAA 3 cut(s) 234, 575, 612
SatI GCNGC 3 cut(s) 174, 395, 990
Sau3AI GATC 7 cut(s) 253, 329, 491, 521, 736, 890, 916
Sau96I GGNCC 2 cut(s) 635, 855
ScrFI CCNGG 1 cut(s) 362
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 2 cut(s) 314, 669
Sfr274I CTCGAG 3 cut(s) 281, 333, 755
SgrAI CRCCGGYG 1 cut(s) 926
SinI GGWCC 1 cut(s) 855
SlaI CTCGAG 3 cut(s) 281, 333, 755
SmiMI CAYNNNNRTG 1 cut(s) 863
SmlI CTYRAG 3 cut(s) 281, 333, 755
SmoI CTYRAG 3 cut(s) 281, 333, 755
Sse9I AATT 4 cut(s) 6, 106, 404, 935
SsiI CCGC 4 cut(s) 268, 380, 436, 528
SspMI CTAG 2 cut(s) 204, 455
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 1 cut(s) 360
StyI CCWWGG 2 cut(s) 60, 323
TaaI ACNGT 2 cut(s) 792, 810
TaqI TCGA 8 cut(s) 76, 83, 143, 185, 282, 334, 555, 756
TasI AATT 4 cut(s) 6, 106, 404, 935
TfiI GAWTC 3 cut(s) 78, 271, 955
Tru1I TTAA 3 cut(s) 234, 575, 612
Tru9I TTAA 3 cut(s) 234, 575, 612
TscAI CASTG 2 cut(s) 676, 813
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 3 cut(s) 173, 394, 989
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 3 cut(s) 56, 288, 881
TspGWI ACGGA 2 cut(s) 42, 877
TspRI CASTG 2 cut(s) 676, 813
Tth111I GACNNNGTC 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 855
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 1 cut(s) 404
XceI RCATGY 2 cut(s) 417, 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XhoI CTCGAG 3 cut(s) 281, 333, 755
XmiI GTMKAC 1 cut(s) 555
XspI CTAG 2 cut(s) 204, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.