Rroxscaffold_3G00229550

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006188
Physical Location & Seq
Forward (+)
14055113 .. 14063382
8270 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229550.1

Sequence Viewer

Length: 753 bp
ATGTCTCTCACGTGCGTCCTCTATTCCTCAAACCCACCTCACACTAAACCTTCCCTATTCCCAACAACTCCCACTCTTCAGCTTTCGACTTTCTTCAGAAATGGCAGCAACTCTGTTTCTCGCAGAAACAACAGATTTCAGTTCCATGCAAACTCCACTGACTCAGACGACGAATTGGGAACTAGGCAAGTTGCTGCCGTTTCCAACCCATCATCGACCAGCCTTCTCTCTTTTCTTTGCCCCTTGCTCAAACTCTTCTCCGGAGGAGATCCCTCACAAGAACGGAACTTTACCTTGGAGGTAGCCACATCTTCCTTGTCTACCTTGGCTAGATTCCCTTGGGGATCAAGATCTCTATCAAATAATTCGGACAGCCAGGAAGTCACTACCTTGGATCCTCCTATGCGTCTGCAGCTTTTTGAATTCGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTATGACTGAGCTAGATCTTTCAGCAGAGGTGTATCCTTGTCCAAAAGGTTCGATAAGACACAGAGAATTGGTTAACAGATCTGGTGGAAAAGAGCAATTTCCTTTTCTCATTGATCCAAATACCAGTATTTCAATGTATGAAAGCGGTGAAATCGTGAGATACCTATTTCAGCAATATGGGAAAGCAGAAAACCTTCACCGGGGCTTTTGGAAAGTACATTGCTTACAGGGTGGATGCCGACAATATTTCGAGCAGGTAGAGGAATGGCATTGTGGGAAAGGGCGACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

250

Amino Acids

28.3

Weight (kDa)

7.6

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 139 - 215 1.3e-18 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 144 - 209 2.1e-07 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 706
AccI GTMKAC 1 cut(s) 320
AccII CGCG 1 cut(s) 456
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 1 cut(s) 606
AclWI GGATC 5 cut(s) 263, 352, 389, 402, 569
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 2 cut(s) 62, 79
AcvI CACGTG 1 cut(s) 12
AfaI GTAC 1 cut(s) 678
AfiI CCNNNNNNNGG 1 cut(s) 661
AgsI TTSAA 2 cut(s) 422, 594
AjnI CCWGG 1 cut(s) 375
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 4 cut(s) 82, 415, 461, 472
AluI AGCT 4 cut(s) 82, 415, 461, 472
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 5 cut(s) 263, 352, 389, 402, 569
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 3 cut(s) 105, 194, 412
ApoI RAATTY 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 654
AsuC2I CCSGG 1 cut(s) 662
AsuHPI GGTGA 2 cut(s) 620, 650
BamHI GGATCC 1 cut(s) 394
BbrPI CACGTG 1 cut(s) 12
BbvI GCAGC 3 cut(s) 117, 181, 424
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 377
BciVI GTATCC 1 cut(s) 504
BcnI CCSGG 1 cut(s) 662
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 183, 330, 473
BfmI CTRYAG 2 cut(s) 410, 749
BfuAI ACCTGC 1 cut(s) 706
BfuI GTATCC 1 cut(s) 504
BglII AGATCT 3 cut(s) 350, 475, 539
BisI GCNGC 3 cut(s) 106, 195, 413
BlsI GCNGC 3 cut(s) 107, 196, 414
Bme1390I CCNGG 2 cut(s) 377, 662
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 377, 662
BmsI GCATC 1 cut(s) 686
BpuMI CCSGG 1 cut(s) 662
BsaAI YACGTR 1 cut(s) 12
BsaBI GATNNNNATC 2 cut(s) 349, 355
BsaJI CCNNGG 5 cut(s) 294, 324, 338, 390, 661
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 661
Bse1I ACTGG 1 cut(s) 585
Bse3DI GCAATG 1 cut(s) 679
Bse8I GATNNNNATC 2 cut(s) 349, 355
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 5 cut(s) 294, 324, 338, 390, 661
BseGI GGATG 1 cut(s) 701
BseJI GATNNNNATC 2 cut(s) 349, 355
BseLI CCNNNNNNNGG 1 cut(s) 661
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 2 cut(s) 177, 459
BseNI ACTGG 1 cut(s) 585
BseRI GAGGAG 1 cut(s) 279
BseXI GCAGC 3 cut(s) 117, 181, 424
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 1 cut(s) 431
BsiSI CCGG 2 cut(s) 261, 661
BslI CCNNNNNNNGG 1 cut(s) 661
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 431
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
Bsp68I TCGCGA 1 cut(s) 456
BspACI CCGC 1 cut(s) 606
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 2 cut(s) 176, 460
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 414
BspMI ACCTGC 1 cut(s) 706
BspPI GGATC 5 cut(s) 263, 352, 389, 402, 569
BsrDI GCAATG 1 cut(s) 679
BsrI ACTGG 1 cut(s) 585
BssECI CCNNGG 5 cut(s) 294, 324, 338, 390, 661
BssMI GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
BssT1I CCWWGG 4 cut(s) 294, 324, 338, 390
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 750
Bst6I CTCTTC 2 cut(s) 81, 260
BstBAI YACGTR 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 163, 468
BstF5I GGATG 1 cut(s) 701
BstFNI CGCG 1 cut(s) 456
BstKTI GATC 7 cut(s) 271, 347, 353, 397, 478, 542, 577
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
BstMWI GCNNNNNNNGC 2 cut(s) 412, 441
BstNI CCWGG 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 375, 660
BstSFI CTRYAG 2 cut(s) 410, 749
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 117, 181, 424
BstX2I RGATCY 5 cut(s) 268, 350, 394, 475, 539
BstYI RGATCY 5 cut(s) 268, 350, 394, 475, 539
BsuI GTATCC 1 cut(s) 504
BsuRI GGCC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 701
BtsIMutI CAGTG 1 cut(s) 156
BtuMI TCGCGA 1 cut(s) 456
BveI ACCTGC 1 cut(s) 706
Cac8I GCNNGC 1 cut(s) 433
CseI GACGC 2 cut(s) 4, 395
Csp6I GTAC 1 cut(s) 677
CviAII CATG 1 cut(s) 146
CviQI GTAC 1 cut(s) 677
DdeI CTNAG 2 cut(s) 163, 468
DpnI GATC 7 cut(s) 270, 346, 352, 396, 477, 541, 576
DpnII GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
Eam1104I CTCTTC 2 cut(s) 81, 260
EarI CTCTTC 2 cut(s) 81, 260
Eco130I CCWWGG 4 cut(s) 294, 324, 338, 390
Eco147I AGGCCT 1 cut(s) 431
Eco57I CTGAAG 2 cut(s) 62, 79
Eco72I CACGTG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 4 cut(s) 294, 324, 338, 390
ErhI CCWWGG 4 cut(s) 294, 324, 338, 390
FaeI CATG 1 cut(s) 149
FaiI YATR 5 cut(s) 147, 404, 464, 600, 639
FatI CATG 1 cut(s) 145
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 3 cut(s) 106, 195, 413
FokI GGATG 1 cut(s) 708
Fsp4HI GCNGC 3 cut(s) 106, 195, 413
FspBI CTAG 3 cut(s) 183, 330, 473
GluI GCNGC 3 cut(s) 106, 195, 413
HaeIII GGCC 1 cut(s) 431
HapII CCGG 2 cut(s) 261, 661
HgaI GACGC 2 cut(s) 4, 395
Hin1II CATG 1 cut(s) 149
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 2 cut(s) 161, 333
HpaI GTTAAC 1 cut(s) 535
HpaII CCGG 2 cut(s) 261, 661
HphI GGTGA 2 cut(s) 620, 650
Hpy166II GTNNAC 2 cut(s) 321, 535
Hpy188I TCNGA 3 cut(s) 98, 166, 370
Hpy188III TCNNGA 4 cut(s) 261, 348, 455, 616
Hpy8I GTNNAC 2 cut(s) 321, 535
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 3 cut(s) 60, 233, 665
HpyCH4III ACNGT 1 cut(s) 750
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 3 cut(s) 149, 412, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 412, 441
HpyF3I CTNAG 2 cut(s) 163, 468
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 1 cut(s) 149
Kpn2I TCCGGA 1 cut(s) 260
KspAI GTTAAC 1 cut(s) 535
Kzo9I GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
Lsp1109I GCAGC 3 cut(s) 117, 181, 424
LweI GCATC 1 cut(s) 686
MaeI CTAG 3 cut(s) 183, 330, 473
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 382
MalI GATC 7 cut(s) 270, 346, 352, 396, 477, 541, 576
MboI GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
MboII GAAGA 4 cut(s) 68, 85, 247, 303
MflI RGATCY 5 cut(s) 268, 350, 394, 475, 539
MluCI AATT 5 cut(s) 173, 364, 422, 527, 557
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 228
MroI TCCGGA 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 1 cut(s) 534
MspI CCGG 2 cut(s) 261, 661
MspR9I CCNGG 2 cut(s) 377, 662
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 412, 441
NciI CCSGG 1 cut(s) 662
NdeII GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
NlaIII CATG 1 cut(s) 149
NlaIV GGNNCC 1 cut(s) 396
NmuCI GTSAC 1 cut(s) 382
NruI TCGCGA 1 cut(s) 456
PceI AGGCCT 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 333
PkrI GCNGC 3 cut(s) 107, 196, 414
PleI GAGTC 1 cut(s) 155
PmaCI CACGTG 1 cut(s) 12
PmlI CACGTG 1 cut(s) 12
PpsI GAGTC 1 cut(s) 155
Ppu21I YACGTR 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 375
PspCI CACGTG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 396
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 5 cut(s) 268, 350, 394, 475, 539
RruI TCGCGA 1 cut(s) 456
RsaI GTAC 1 cut(s) 678
RsaNI GTAC 1 cut(s) 677
SaqAI TTAA 1 cut(s) 534
SatI GCNGC 3 cut(s) 106, 195, 413
Sau3AI GATC 7 cut(s) 268, 344, 350, 394, 475, 539, 574
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 377, 662
SfaNI GCATC 1 cut(s) 686
SfcI CTRYAG 2 cut(s) 410, 749
Sse9I AATT 5 cut(s) 173, 364, 422, 527, 557
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 1 cut(s) 606
SspI AATATT 1 cut(s) 707
SspMI CTAG 3 cut(s) 183, 330, 473
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 2 cut(s) 375, 660
StyI CCWWGG 4 cut(s) 294, 324, 338, 390
TaaI ACNGT 1 cut(s) 750
TaiI ACGT 1 cut(s) 14
TaqI TCGA 5 cut(s) 86, 215, 426, 512, 711
TasI AATT 5 cut(s) 173, 364, 422, 527, 557
TatI WGTACW 1 cut(s) 676
TfiI GAWTC 1 cut(s) 333
Tru1I TTAA 1 cut(s) 534
Tru9I TTAA 1 cut(s) 534
TscAI CASTG 1 cut(s) 163
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 3 cut(s) 105, 194, 412
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 1 cut(s) 615
TspGWI ACGGA 1 cut(s) 298
TspRI CASTG 1 cut(s) 163
XapI RAATTY 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 320
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 3 cut(s) 183, 330, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.