pycom15g35830

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0006188
Physical Location & Seq
Forward (+)
35918255 .. 35922360
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35830.5

Sequence Viewer

Length: 1224 bp
ATGTATCTCGAAGTACAAAGATGGAGAGAGATTTTTAAAGATTGCGAAGCGAAAGCAAGCACTGATAACCACCCAACAGGCAACAACTCAACTGAAACAGGAGAGAAGCAAGGTCCAAACATGTCACTAGCATCTCTGACGTCGTGCTCTCAAACACTAGTCTACTACTCTTCCTCTAAGCCGCCGCCTTCTCCGCCTCAGCATCCGCCTCCGCCATACAGTTTCCTCACACATGCCCACAATTCTCCATTAATTCCAACCACTTTAATTCCAGTTTCTACCCTCAAACTATCTCCATGTTCTCCAACATTGCTGACAAATGGGAACTGGGTTCCTCGCAGAAACAGATTTCTTCCTGCAAATTCAGCCGACTCCGACGCACAAAACGAATCGGCAACCGGAGAAAATGGCGGCAACCCATCAACCAGCCTTCTGTCTTTTCTTTGTCCCCTGCTCAAGCTCTTCTCCGGAGGAGATCCCTCTCAAGAACGGAACTTTACTTTGGAGGTAGCTACGTCTTCGTTGTCTACCTTGGCTAGATTCCCTTGGGGATCAAGATCTCTGTCAAATAATATGGACAGCCAGGAGATCACTACCTTGGATCCTCCTTTGCGTTTGCAGCTTTTTGAATTTGAGGCTTGCCCTTTTTGCAGGAGGGTTAGAGAAGCTTTGACTGAGCTTGATCTTTCGGTAGAGGTATATCCTTGTCCAAAAGGTTCGGTAAGACATAGAGAAATGGTCAAAAGATTCGGTGGCAAAGAGCTGTTTCCTTTTCTCATTGATCCAAATACTGGTATTTCAATGTATGAAAGTGGTGAAATTGTGAAATACCTATTCCAGCAATATGGTAAAGGCAGAAAGCCTTCAGTGGGGCTTTTGGAAAGCACATTATTCACGGGATGGATGCCAACAATTCTGCGAGCAGGCAGAGGATTGACATTGTGGGAAAAGGCGAGCCCAGACCCACCACCCAAAAAGCTGGAACTTTACTCGTACGAAAACAATCCGTATGCACGAATTGTGCGTGAGGCACTTTGTGAATTGGAACTTCCTTACATCCTTCAAAACGTGGGAGAAGGGTCCCTGCGGTCGAAGTTACTTGAGGCATCTGGATCCAAAGAGGTTCCTTACCTGATTGATCCTAACACCAGTACTCATGTCGGTGACTACAAGAAGATTCTGTCGTACTTGTTCCAGACATATTCAGCAGCCACTCTTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

408

Amino Acids

45.24

Weight (kDa)

6.62

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 791
AccI GTMKAC 2 cut(s) 162, 527
AccIII TCCGGA 1 cut(s) 467
AciI CCGC 7 cut(s) 182, 185, 194, 206, 212, 411, 1087
AclWI GGATC 8 cut(s) 470, 559, 596, 609, 776, 1107, 1120, 1133
AcsI RAATTY 2 cut(s) 361, 629
AcuI CTGAAG 1 cut(s) 849
AcyI GRCGYC 1 cut(s) 140
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 4 cut(s) 15, 995, 1153, 1187
AfiI CCNNNNNNNGG 2 cut(s) 791, 869
AflIII ACRYGT 1 cut(s) 120
AgsI TTSAA 3 cut(s) 629, 801, 1064
AhlI ACTAGT 1 cut(s) 157
AjnI CCWGG 1 cut(s) 582
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 7 cut(s) 460, 512, 622, 668, 679, 763, 979
AluI AGCT 7 cut(s) 460, 512, 622, 668, 679, 763, 979
Alw21I GWGCWC 1 cut(s) 149
AlwI GGATC 8 cut(s) 470, 559, 596, 609, 776, 1107, 1120, 1133
Aor13HI TCCGGA 1 cut(s) 467
ApeKI GCWGC 2 cut(s) 619, 1208
ApoI RAATTY 2 cut(s) 361, 629
AseI ATTAAT 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 862
AspS9I GGNCC 2 cut(s) 113, 1080
AsuHPI GGTGA 2 cut(s) 827, 1175
AvaII GGWCC 2 cut(s) 113, 1080
BamHI GGATCC 2 cut(s) 601, 1112
BanII GRGCYC 1 cut(s) 959
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 198
BbvI GCAGC 2 cut(s) 631, 1220
BccI CCATC 3 cut(s) 15, 427, 894
BciT130I CCWGG 1 cut(s) 584
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 3 cut(s) 128, 158, 537
BglII AGATCT 1 cut(s) 557
BisI GCNGC 5 cut(s) 182, 185, 412, 620, 1209
BlsI GCNGC 5 cut(s) 183, 186, 413, 621, 1210
BmcAI AGTACT 1 cut(s) 1153
Bme1390I CCNGG 1 cut(s) 584
Bme18I GGWCC 2 cut(s) 113, 1080
BmgT120I GGNCC 2 cut(s) 113, 1080
BmiI GGNNCC 6 cut(s) 333, 603, 1081, 1082, 1114, 1125
BmrFI CCNGG 1 cut(s) 584
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 4 cut(s) 140, 211, 894, 1115
BmuI ACTGGG 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 198
BpuEI CTTGAG 3 cut(s) 440, 468, 1121
BsaBI GATNNNNATC 1 cut(s) 556
BsaHI GRCGYC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 531, 545, 597
BsaWI WCCGGW 2 cut(s) 398, 467
Bsc4I CCNNNNNNNGG 2 cut(s) 791, 869
Bse1I ACTGG 4 cut(s) 272, 332, 796, 1149
Bse3DI GCAATG 1 cut(s) 308
Bse8I GATNNNNATC 1 cut(s) 556
BseAI TCCGGA 1 cut(s) 467
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 3 cut(s) 531, 545, 597
BseGI GGATG 4 cut(s) 202, 905, 909, 1056
BseJI GATNNNNATC 1 cut(s) 556
BseLI CCNNNNNNNGG 2 cut(s) 791, 869
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 212, 666
BseNI ACTGG 4 cut(s) 272, 332, 796, 1149
BseRI GAGGAG 1 cut(s) 486
BseXI GCAGC 2 cut(s) 631, 1220
Bsh1285I CGRYCG 1 cut(s) 1091
BsiEI CGRYCG 1 cut(s) 1091
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 2 cut(s) 399, 468
BsiWI CGTACG 1 cut(s) 993
BslFI GGGAC 2 cut(s) 432, 1066
BslI CCNNNNNNNGG 2 cut(s) 791, 869
BsmFI GGGAC 2 cut(s) 432, 1066
Bsp1286I GDGCHC 2 cut(s) 149, 959
Bsp13I TCCGGA 1 cut(s) 467
Bsp143I GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
BspACI CCGC 7 cut(s) 182, 185, 194, 206, 212, 411, 1087
BspCNI CTCAG 2 cut(s) 211, 667
BspEI TCCGGA 1 cut(s) 467
BspLI GGNNCC 6 cut(s) 333, 603, 1081, 1082, 1114, 1125
BspPI GGATC 8 cut(s) 470, 559, 596, 609, 776, 1107, 1120, 1133
BspQI GCTCTTC 1 cut(s) 467
BsrDI GCAATG 1 cut(s) 308
BsrI ACTGG 4 cut(s) 272, 332, 796, 1149
BssECI CCNNGG 3 cut(s) 531, 545, 597
BssMI GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 3 cut(s) 531, 545, 597
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 1 cut(s) 221
Bst6I CTCTTC 2 cut(s) 175, 467
BstACI GRCGYC 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 58, 640, 921, 925, 955
BstDEI CTNAG 3 cut(s) 177, 198, 675
BstF5I GGATG 4 cut(s) 202, 905, 909, 1056
BstKTI GATC 9 cut(s) 478, 554, 560, 591, 604, 685, 784, 1115, 1141
BstMBI GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
BstMCI CGRYCG 1 cut(s) 1091
BstMWI GCNNNNNNNGC 4 cut(s) 193, 365, 619, 648
BstNI CCWGG 1 cut(s) 584
BstNSI RCATGY 2 cut(s) 124, 236
BstSCI CCNGG 1 cut(s) 582
BstV1I GCAGC 2 cut(s) 631, 1220
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 4 cut(s) 475, 557, 601, 1112
BstXI CCANNNNNNTGG 2 cut(s) 845, 979
BstYI RGATCY 4 cut(s) 475, 557, 601, 1112
BtsCI GGATG 4 cut(s) 202, 905, 909, 1056
BtsIMutI CAGTG 2 cut(s) 60, 873
Cac8I GCNNGC 5 cut(s) 58, 640, 921, 925, 955
Cfr13I GGNCC 2 cut(s) 113, 1080
CseI GACGC 1 cut(s) 386
Csp6I GTAC 4 cut(s) 14, 994, 1152, 1186
CviAII CATG 4 cut(s) 121, 233, 297, 1157
CviQI GTAC 4 cut(s) 14, 994, 1152, 1186
DdeI CTNAG 3 cut(s) 177, 198, 675
DpnI GATC 9 cut(s) 477, 553, 559, 590, 603, 684, 783, 1114, 1140
DpnII GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
DraI TTTAAA 1 cut(s) 37
DraIII CACNNNGTG 1 cut(s) 1037
Eam1104I CTCTTC 2 cut(s) 175, 467
EarI CTCTTC 2 cut(s) 175, 467
EciI GGCGGA 3 cut(s) 183, 195, 201
Eco130I CCWWGG 3 cut(s) 531, 545, 597
Eco24I GRGCYC 1 cut(s) 959
Eco47I GGWCC 2 cut(s) 113, 1080
Eco57I CTGAAG 1 cut(s) 849
EcoO109I RGGNCCY 1 cut(s) 1080
EcoRII CCWGG 1 cut(s) 582
EcoT14I CCWWGG 3 cut(s) 531, 545, 597
EcoT38I GRGCYC 1 cut(s) 959
ErhI CCWWGG 3 cut(s) 531, 545, 597
FaeI CATG 4 cut(s) 124, 236, 300, 1160
FaqI GGGAC 2 cut(s) 432, 1066
FatI CATG 4 cut(s) 120, 232, 296, 1156
FblI GTMKAC 2 cut(s) 162, 527
Fnu4HI GCNGC 5 cut(s) 182, 185, 412, 620, 1209
FokI GGATG 4 cut(s) 189, 912, 916, 1043
FriOI GRGCYC 1 cut(s) 959
Fsp4HI GCNGC 5 cut(s) 182, 185, 412, 620, 1209
FspBI CTAG 3 cut(s) 128, 158, 537
GluI GCNGC 5 cut(s) 182, 185, 412, 620, 1209
HapII CCGG 2 cut(s) 399, 468
HgaI GACGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 4 cut(s) 124, 236, 300, 1160
HindIII AAGCTT 1 cut(s) 666
HinfI GANTC 5 cut(s) 371, 389, 540, 747, 1177
HpaII CCGG 2 cut(s) 399, 468
HphI GGTGA 2 cut(s) 827, 1175
Hpy166II GTNNAC 2 cut(s) 163, 528
Hpy188I TCNGA 2 cut(s) 138, 376
Hpy188III TCNNGA 6 cut(s) 8, 468, 485, 555, 1110, 1195
Hpy8I GTNNAC 2 cut(s) 163, 528
Hpy99I CGWCG 2 cut(s) 145, 380
HpyAV CCTTC 5 cut(s) 198, 440, 873, 1070, 1070
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 3 cut(s) 140, 515, 1068
HpyCH4V TGCA 4 cut(s) 359, 619, 651, 1013
HpyF10VI GCNNNNNNNGC 4 cut(s) 193, 365, 619, 648
HpyF3I CTNAG 3 cut(s) 177, 198, 675
HpySE526I ACGT 3 cut(s) 140, 515, 1068
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 4 cut(s) 124, 236, 300, 1160
KflI GGGWCCC 1 cut(s) 1080
Kpn2I TCCGGA 1 cut(s) 467
Kzo9I GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
LguI GCTCTTC 1 cut(s) 467
Lsp1109I GCAGC 2 cut(s) 631, 1220
LweI GCATC 4 cut(s) 140, 211, 894, 1115
MaeI CTAG 3 cut(s) 128, 158, 537
MaeII ACGT 3 cut(s) 140, 515, 1068
MaeIII GTNAC 3 cut(s) 123, 1095, 1163
MalI GATC 9 cut(s) 477, 553, 559, 590, 603, 684, 783, 1114, 1140
MboI GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
MboII GAAGA 5 cut(s) 162, 344, 454, 510, 1186
MflI RGATCY 4 cut(s) 475, 557, 601, 1112
MhlI GDGCHC 2 cut(s) 149, 959
MluCI AATT 9 cut(s) 241, 252, 267, 361, 629, 819, 912, 1017, 1040
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 3 cut(s) 281, 329, 399
MroI TCCGGA 1 cut(s) 467
MroXI GAANNNNTTC 1 cut(s) 862
MseI TTAA 3 cut(s) 36, 251, 266
MslI CAYNNNNRTG 1 cut(s) 1161
MspI CCGG 2 cut(s) 399, 468
MspR9I CCNGG 1 cut(s) 584
MvaI CCWGG 1 cut(s) 584
MwoI GCNNNNNNNGC 4 cut(s) 193, 365, 619, 648
NdeII GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
NlaIII CATG 4 cut(s) 124, 236, 300, 1160
NlaIV GGNNCC 6 cut(s) 333, 603, 1081, 1082, 1114, 1125
NmuCI GTSAC 2 cut(s) 123, 1163
NspI RCATGY 2 cut(s) 124, 236
PciI ACATGT 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 2 cut(s) 384, 1021
PdmI GAANNNNTTC 1 cut(s) 862
PfeI GAWTC 4 cut(s) 389, 540, 747, 1177
Pfl23II CGTACG 1 cut(s) 993
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 5 cut(s) 183, 186, 413, 621, 1210
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 1080
PscI ACATGT 1 cut(s) 120
PshBI ATTAAT 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 1080
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspLI CGTACG 1 cut(s) 993
PspN4I GGNNCC 6 cut(s) 333, 603, 1081, 1082, 1114, 1125
PspPI GGNCC 2 cut(s) 113, 1080
PspPPI RGGWCCY 1 cut(s) 1080
PsuI RGATCY 4 cut(s) 475, 557, 601, 1112
RsaI GTAC 4 cut(s) 15, 995, 1153, 1187
RsaNI GTAC 4 cut(s) 14, 994, 1152, 1186
RseI CAYNNNNRTG 1 cut(s) 1161
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 3 cut(s) 36, 251, 266
SatI GCNGC 5 cut(s) 182, 185, 412, 620, 1209
Sau3AI GATC 9 cut(s) 475, 551, 557, 588, 601, 682, 781, 1112, 1138
Sau96I GGNCC 2 cut(s) 113, 1080
ScaI AGTACT 1 cut(s) 1153
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 2 cut(s) 149, 959
SfaNI GCATC 4 cut(s) 140, 211, 894, 1115
SinI GGWCC 2 cut(s) 113, 1080
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 3 cut(s) 455, 483, 1100
SmoI CTYRAG 3 cut(s) 455, 483, 1100
SpeI ACTAGT 1 cut(s) 157
Sse9I AATT 9 cut(s) 241, 252, 267, 361, 629, 819, 912, 1017, 1040
SsiI CCGC 7 cut(s) 182, 185, 194, 206, 212, 411, 1087
SspMI CTAG 3 cut(s) 128, 158, 537
StyD4I CCNGG 1 cut(s) 582
StyI CCWWGG 3 cut(s) 531, 545, 597
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 3 cut(s) 143, 518, 1071
TaqI TCGA 2 cut(s) 9, 1091
TasI AATT 9 cut(s) 241, 252, 267, 361, 629, 819, 912, 1017, 1040
TatI WGTACW 2 cut(s) 13, 1151
TauI GCSGC 3 cut(s) 184, 187, 414
TfiI GAWTC 4 cut(s) 389, 540, 747, 1177
Tru1I TTAA 3 cut(s) 36, 251, 266
Tru9I TTAA 3 cut(s) 36, 251, 266
TscAI CASTG 2 cut(s) 67, 873
TseFI GTSAC 2 cut(s) 123, 1163
TseI GCWGC 2 cut(s) 619, 1208
Tsp45I GTSAC 2 cut(s) 123, 1163
TspDTI ATGAA 1 cut(s) 822
TspGWI ACGGA 2 cut(s) 505, 996
TspRI CASTG 2 cut(s) 67, 873
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 2 cut(s) 113, 1080
VspI ATTAAT 1 cut(s) 251
XapI RAATTY 2 cut(s) 361, 629
XceI RCATGY 2 cut(s) 124, 236
XmiI GTMKAC 2 cut(s) 162, 527
XmnI GAANNNNTTC 1 cut(s) 862
XspI CTAG 3 cut(s) 128, 158, 537
ZraI GACGTC 1 cut(s) 141
ZrmI AGTACT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.