RLG00000001433

Glutathione S-transferase, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006188
Physical Location & Seq
Forward (+)
15560120 .. 15569851
9732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001433

Sequence Viewer

Length: 1368 bp
ATGTCTCTAACATTCTCTCAAACACTAGTCCTCTATTCCTCAGACCCACCTCACAGTTGTCTCCCGAAACCATCCCTATTCCCAACAACTTCAATTCCGAAGCTATCACCAACATTCTACAGAAATGGCAGCAACTCTGTTTCTCGCAGAAACAACAGATTTCAGTTCCATGCAAACTCCACTGACTCAGACGACGAATTGGGAACTAGGCAAGTTGCTGCCGTTTCCAACCCATCATCCACCAGCCTTCTCTCTTTTCTTTGCCCCTTGCTTAAACTCTTCTCCGGAGGAGATCCCTCACAAGAACGGAACTTTACCTTGGAGGTAGCCACATCTTCCTTGTCTACCTTGGCTAGATTCCCTTGGGGATCAAGATCTCTATCCAATAATTTGGACAGCCAGGAAGTCACTACCTTGGATCCTCCTCTGCGTCTGCAGCTTTTTGAATTCGAGGCCTGCCCTTTTTGCAGGAGGGTTCGCGAAGCTATGACTGAGCTAGATCTATCAGCAGAGGTTTATCCTTGTCCAAAAGGTTCGATAAGACACAGAGAATTGGTTAACAGATCTGGTGGAAAAGAGCAATTTCCTTTTCTCATTGATCCAAATACCGGTATTTCAATGTATGAAAGCGGTGAAATCGTGAGATATCTATTTCAGCAATATGGGAAAGGCAGAAAACCTTCACCAGGGCTTTTGGAAAGTACATTGCTTACAGGGTGGATGCCGACAATATTTCGAGCAGGTAGAGGAATGACATTATGGGAAAGGGCGACTGTAGAGCCACCACCTGAAAAGCTGGAACTTTACTCGTACGAAAATAATCCGTATGCACGAATTGTGCGTGAGGCACTTTGTGAGTTGGAGCTTCCTTACATCCTACAGAATGTAGGAGAAGGGTCCCTGCGGTTAAAGCTGCTTGTTGATGCATCGGGGTCCAAAGAGGTTCCCTACTTGATTGATCCCAACACTGGTACTCATGTTGGTGACTACAAGAAGATCCTGTCCTACTTGTTCCAGACATATTCAGTAGCCGCTGTTGGAATTGATACGAAAAGTAGAGTTAGCAGCTCGGTCAATGATAGTCAATGGGTTTGGCCAAGCTCTACAGCCAAGATCAGCATAGGTAAAAGGTTATTCATGGATGTCACTGCTCCTAATGCCAACAATAGGGATAATAAGAGGTGGTGGTGCCAAGTGAGGCTAGGGTTTGCGAGTCAAGGAGTCATGGATTCCATGGGACATGTATTCACCACAACAACTGTGCTCTCGTCTGCCGTAACGAAGGCTTCCCCGGTGGTAGATGCCGTGGATTCCGTCGCCGCTGCTTTTGCACTCGAAATTGTTGAAGCCTATAGCATCTCTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko04914 ko05166 map04110 map04111 map04113 map04114 map04120 map04914 map05166
Pfam Domains
Protein Families
GST_N_3

Protein Analysis

456

Amino Acids

50.41

Weight (kDa)

6.96

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 147 - 222 2e-18 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 152 - 217 5.6e-07 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 268 - 342 8.5e-07 Glutathione S-transferase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 731
AccB1I GGYRCC 1 cut(s) 1188
AccI GTMKAC 1 cut(s) 344
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 4 cut(s) 630, 904, 1032, 1320
AclWI GGATC 7 cut(s) 287, 376, 413, 426, 593, 953, 991
AcoI YGGCCR 1 cut(s) 1094
AcsI RAATTY 1 cut(s) 446
AdeI CACNNNGTG 1 cut(s) 854
AfaI GTAC 3 cut(s) 703, 812, 973
AfiI CCNNNNNNNGG 4 cut(s) 608, 686, 968, 1167
AflIII ACRYGT 1 cut(s) 1240
AgeI ACCGGT 1 cut(s) 608
AgsI TTSAA 4 cut(s) 93, 446, 618, 1346
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 399, 685
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
Alw21I GWGCWC 1 cut(s) 1266
Alw26I GTCTC 2 cut(s) 9, 65
AlwI GGATC 7 cut(s) 287, 376, 413, 426, 593, 953, 991
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 2 cut(s) 453, 1094
ApeKI GCWGC 6 cut(s) 129, 218, 436, 913, 1065, 1322
ApoI RAATTY 1 cut(s) 446
AsiGI ACCGGT 1 cut(s) 608
Asp700I GAANNNNTTC 1 cut(s) 679
AspS9I GGNCC 2 cut(s) 897, 933
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 5 cut(s) 99, 644, 675, 995, 1240
AvaII GGWCC 2 cut(s) 897, 933
BaeI ACNNNNGTAYC 2 cut(s) 963, 996
BalI TGGCCA 1 cut(s) 1096
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 1188
Bbv12I GWGCWC 1 cut(s) 1266
BbvI GCAGC 6 cut(s) 141, 205, 448, 900, 1077, 1309
BccI CCATC 2 cut(s) 79, 241
BceAI ACGGC 3 cut(s) 206, 1259, 1289
BciT130I CCWGG 2 cut(s) 401, 687
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 2 cut(s) 9, 65
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 6 cut(s) 26, 207, 354, 497, 1202, 1362
BfmI CTRYAG 6 cut(s) 118, 434, 774, 878, 1104, 1351
BfuAI ACCTGC 1 cut(s) 731
BglII AGATCT 3 cut(s) 374, 499, 563
BisI GCNGC 8 cut(s) 130, 219, 437, 914, 1032, 1066, 1320, 1323
BlsI GCNGC 8 cut(s) 131, 220, 438, 915, 1033, 1067, 1321, 1324
Bme1390I CCNGG 3 cut(s) 401, 687, 1292
Bme18I GGWCC 2 cut(s) 897, 933
BmgT120I GGNCC 2 cut(s) 897, 933
BmiI GGNNCC 6 cut(s) 420, 898, 899, 934, 945, 1190
BmrFI CCNGG 3 cut(s) 401, 687, 1292
BmsI GCATC 4 cut(s) 711, 913, 935, 1291
BpuMI CCSGG 1 cut(s) 1292
BsaBI GATNNNNATC 2 cut(s) 373, 379
BsaJI CCNNGG 8 cut(s) 318, 348, 362, 414, 686, 1233, 1290, 1305
BsaWI WCCGGW 2 cut(s) 284, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 608, 686, 968, 1167
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 1 cut(s) 973
Bse3DI GCAATG 1 cut(s) 704
Bse8I GATNNNNATC 2 cut(s) 373, 379
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 2 cut(s) 401, 687
BseDI CCNNGG 8 cut(s) 318, 348, 362, 414, 686, 1233, 1290, 1305
BseGI GGATG 5 cut(s) 71, 236, 726, 873, 1147
BseJI GATNNNNATC 2 cut(s) 373, 379
BseLI CCNNNNNNNGG 4 cut(s) 608, 686, 968, 1167
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 3 cut(s) 54, 201, 483
BseNI ACTGG 1 cut(s) 973
BseRI GAGGAG 2 cut(s) 303, 414
BseXI GCAGC 6 cut(s) 141, 205, 448, 900, 1077, 1309
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 2 cut(s) 455, 1096
BshNI GGYRCC 1 cut(s) 1188
BshTI ACCGGT 1 cut(s) 608
BsiHKAI GWGCWC 1 cut(s) 1266
BsiSI CCGG 3 cut(s) 285, 609, 1292
BsiWI CGTACG 1 cut(s) 810
BslFI GGGAC 2 cut(s) 883, 1251
BslI CCNNNNNNNGG 4 cut(s) 608, 686, 968, 1167
BsmAI GTCTC 2 cut(s) 9, 65
BsmFI GGGAC 2 cut(s) 883, 1251
BsnI GGCC 2 cut(s) 455, 1096
Bsp1286I GDGCHC 1 cut(s) 1266
Bsp13I TCCGGA 1 cut(s) 284
Bsp19I CCATGG 1 cut(s) 1233
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 4 cut(s) 630, 904, 1032, 1320
BspANI GGCC 2 cut(s) 455, 1096
BspCNI CTCAG 3 cut(s) 53, 200, 484
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 6 cut(s) 420, 898, 899, 934, 945, 1190
BspMAI CTGCAG 1 cut(s) 438
BspMI ACCTGC 1 cut(s) 731
BspPI GGATC 7 cut(s) 287, 376, 413, 426, 593, 953, 991
BspT107I GGYRCC 1 cut(s) 1188
BsrDI GCAATG 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 608
BsrI ACTGG 1 cut(s) 973
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 8 cut(s) 318, 348, 362, 414, 686, 1233, 1290, 1305
BssT1I CCWWGG 5 cut(s) 318, 348, 362, 414, 1233
Bst2UI CCWGG 2 cut(s) 401, 687
Bst4CI ACNGT 3 cut(s) 56, 775, 1261
Bst6I CTCTTC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 457
BstDEI CTNAG 3 cut(s) 40, 187, 492
BstDSI CCRYGG 2 cut(s) 1233, 1305
BstENI CCTNNNNNAGG 1 cut(s) 684
BstF5I GGATG 5 cut(s) 71, 236, 726, 873, 1147
BstFNI CGCG 1 cut(s) 480
BstMAI GTCTC 2 cut(s) 9, 65
BstMWI GCNNNNNNNGC 6 cut(s) 436, 465, 910, 1157, 1328, 1362
BstNI CCWGG 2 cut(s) 401, 687
BstNSI RCATGY 1 cut(s) 1244
BstSCI CCNGG 3 cut(s) 399, 685, 1290
BstSFI CTRYAG 6 cut(s) 118, 434, 774, 878, 1104, 1351
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 6 cut(s) 141, 205, 448, 900, 1077, 1309
BstX2I RGATCY 6 cut(s) 292, 374, 418, 499, 563, 996
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 6 cut(s) 292, 374, 418, 499, 563, 996
BsuRI GGCC 2 cut(s) 455, 1096
BtgI CCRYGG 2 cut(s) 1233, 1305
BtsCI GGATG 5 cut(s) 71, 236, 726, 873, 1147
BtsI GCAGTG 1 cut(s) 1146
BtsIMutI CAGTG 3 cut(s) 180, 966, 1146
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 1 cut(s) 731
Cac8I GCNNGC 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 2 cut(s) 897, 933
CseI GACGC 1 cut(s) 419
Csp6I GTAC 3 cut(s) 702, 811, 972
CspAI ACCGGT 1 cut(s) 608
CviAII CATG 6 cut(s) 170, 977, 1138, 1225, 1234, 1241
CviQI GTAC 3 cut(s) 702, 811, 972
DdeI CTNAG 3 cut(s) 40, 187, 492
DraIII CACNNNGTG 1 cut(s) 854
EaeI YGGCCR 1 cut(s) 1094
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco130I CCWWGG 5 cut(s) 318, 348, 362, 414, 1233
Eco147I AGGCCT 1 cut(s) 455
Eco32I GATATC 1 cut(s) 647
Eco47I GGWCC 2 cut(s) 897, 933
EcoNI CCTNNNNNAGG 1 cut(s) 684
EcoO109I RGGNCCY 1 cut(s) 897
EcoRI GAATTC 1 cut(s) 446
EcoRII CCWGG 2 cut(s) 399, 685
EcoRV GATATC 1 cut(s) 647
EcoT14I CCWWGG 5 cut(s) 318, 348, 362, 414, 1233
EcoT22I ATGCAT 1 cut(s) 928
ErhI CCWWGG 5 cut(s) 318, 348, 362, 414, 1233
FaeI CATG 6 cut(s) 173, 980, 1141, 1228, 1237, 1244
FaqI GGGAC 2 cut(s) 883, 1251
FatI CATG 6 cut(s) 169, 976, 1137, 1224, 1233, 1240
FblI GTMKAC 1 cut(s) 344
Fnu4HI GCNGC 8 cut(s) 130, 219, 437, 914, 1032, 1066, 1320, 1323
FokI GGATG 5 cut(s) 58, 223, 733, 860, 1154
Fsp4HI GCNGC 8 cut(s) 130, 219, 437, 914, 1032, 1066, 1320, 1323
FspBI CTAG 6 cut(s) 26, 207, 354, 497, 1202, 1362
GluI GCNGC 8 cut(s) 130, 219, 437, 914, 1032, 1066, 1320, 1323
HaeIII GGCC 2 cut(s) 455, 1096
HapII CCGG 3 cut(s) 285, 609, 1292
HgaI GACGC 1 cut(s) 419
Hin1II CATG 6 cut(s) 173, 980, 1141, 1228, 1237, 1244
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 6 cut(s) 185, 357, 1213, 1221, 1229, 1310
HpaI GTTAAC 1 cut(s) 559
HpaII CCGG 3 cut(s) 285, 609, 1292
HphI GGTGA 5 cut(s) 99, 644, 675, 995, 1240
Hpy166II GTNNAC 2 cut(s) 345, 559
Hpy188I TCNGA 3 cut(s) 43, 99, 190
Hpy188III TCNNGA 6 cut(s) 64, 285, 372, 479, 640, 1015
Hpy8I GTNNAC 2 cut(s) 345, 559
Hpy99I CGWCG 2 cut(s) 197, 1319
HpyAV CCTTC 4 cut(s) 257, 690, 887, 1276
HpyCH4III ACNGT 3 cut(s) 56, 775, 1261
HpyCH4V TGCA 6 cut(s) 173, 436, 468, 830, 926, 1331
HpyF10VI GCNNNNNNNGC 6 cut(s) 436, 465, 910, 1157, 1328, 1362
HpyF3I CTNAG 3 cut(s) 40, 187, 492
Hsp92II CATG 6 cut(s) 173, 980, 1141, 1228, 1237, 1244
KflI GGGWCCC 1 cut(s) 897
Kpn2I TCCGGA 1 cut(s) 284
KspAI GTTAAC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 862, 1156
Lsp1109I GCAGC 6 cut(s) 141, 205, 448, 900, 1077, 1309
LweI GCATC 4 cut(s) 711, 913, 935, 1291
MaeI CTAG 6 cut(s) 26, 207, 354, 497, 1202, 1362
MaeIII GTNAC 4 cut(s) 406, 983, 1144, 1276
MboII GAAGA 3 cut(s) 271, 327, 1006
MflI RGATCY 6 cut(s) 292, 374, 418, 499, 563, 996
MhlI GDGCHC 1 cut(s) 1266
MlsI TGGCCA 1 cut(s) 1096
MluCI AATT 9 cut(s) 93, 197, 388, 446, 551, 581, 834, 1041, 1338
MluNI TGGCCA 1 cut(s) 1096
MlyI GAGTC 3 cut(s) 179, 1222, 1230
MmeI TCCRAC 3 cut(s) 252, 840, 1018
Mox20I TGGCCA 1 cut(s) 1096
Mph1103I ATGCAT 1 cut(s) 928
MroI TCCGGA 1 cut(s) 284
MroXI GAANNNNTTC 1 cut(s) 679
MscI TGGCCA 1 cut(s) 1096
MseI TTAA 3 cut(s) 273, 558, 908
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 1 cut(s) 1096
MspA1I CMGCKG 2 cut(s) 1034, 1322
MspI CCGG 3 cut(s) 285, 609, 1292
MspR9I CCNGG 3 cut(s) 401, 687, 1292
MvaI CCWGG 2 cut(s) 401, 687
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 6 cut(s) 436, 465, 910, 1157, 1328, 1362
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 1233
NlaIII CATG 6 cut(s) 173, 980, 1141, 1228, 1237, 1244
NlaIV GGNNCC 6 cut(s) 420, 898, 899, 934, 945, 1190
NmuCI GTSAC 3 cut(s) 406, 983, 1144
NruI TCGCGA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 928
NspI RCATGY 1 cut(s) 1244
PceI AGGCCT 1 cut(s) 455
PciI ACATGT 1 cut(s) 1240
PcsI WCGNNNNNNNCGW 1 cut(s) 838
PdmI GAANNNNTTC 1 cut(s) 679
PfeI GAWTC 3 cut(s) 357, 1229, 1310
Pfl23II CGTACG 1 cut(s) 810
PinAI ACCGGT 1 cut(s) 608
PkrI GCNGC 8 cut(s) 131, 220, 438, 915, 1033, 1067, 1321, 1324
PleI GAGTC 3 cut(s) 179, 1221, 1229
PpsI GAGTC 3 cut(s) 179, 1221, 1229
PpuMI RGGWCCY 1 cut(s) 897
PscI ACATGT 1 cut(s) 1240
Psp5II RGGWCCY 1 cut(s) 897
Psp6I CCWGG 2 cut(s) 399, 685
PspGI CCWGG 2 cut(s) 399, 685
PspLI CGTACG 1 cut(s) 810
PspN4I GGNNCC 6 cut(s) 420, 898, 899, 934, 945, 1190
PspPI GGNCC 2 cut(s) 897, 933
PspPPI RGGWCCY 1 cut(s) 897
PstI CTGCAG 1 cut(s) 438
PsuI RGATCY 6 cut(s) 292, 374, 418, 499, 563, 996
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 3 cut(s) 703, 812, 973
RsaNI GTAC 3 cut(s) 702, 811, 972
RseI CAYNNNNRTG 1 cut(s) 981
SaqAI TTAA 3 cut(s) 273, 558, 908
SatI GCNGC 8 cut(s) 130, 219, 437, 914, 1032, 1066, 1320, 1323
Sau96I GGNCC 2 cut(s) 897, 933
SchI GAGTC 3 cut(s) 179, 1222, 1230
ScrFI CCNGG 3 cut(s) 401, 687, 1292
SduI GDGCHC 1 cut(s) 1266
SfaNI GCATC 4 cut(s) 711, 913, 935, 1291
SfcI CTRYAG 6 cut(s) 118, 434, 774, 878, 1104, 1351
SinI GGWCC 2 cut(s) 897, 933
SmiMI CAYNNNNRTG 1 cut(s) 981
SpeI ACTAGT 1 cut(s) 25
Sse9I AATT 9 cut(s) 93, 197, 388, 446, 551, 581, 834, 1041, 1338
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 4 cut(s) 630, 904, 1032, 1320
SspI AATATT 1 cut(s) 732
SspMI CTAG 6 cut(s) 26, 207, 354, 497, 1202, 1362
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 399, 685, 1290
StyI CCWWGG 5 cut(s) 318, 348, 362, 414, 1233
TaaI ACNGT 3 cut(s) 56, 775, 1261
TaqI TCGA 4 cut(s) 450, 536, 736, 1335
TaqII GACCGA 1 cut(s) 1060
TasI AATT 9 cut(s) 93, 197, 388, 446, 551, 581, 834, 1041, 1338
TatI WGTACW 1 cut(s) 701
TauI GCSGC 2 cut(s) 1034, 1322
TfiI GAWTC 3 cut(s) 357, 1229, 1310
Tru1I TTAA 3 cut(s) 273, 558, 908
Tru9I TTAA 3 cut(s) 273, 558, 908
TscAI CASTG 3 cut(s) 187, 973, 1153
TseFI GTSAC 3 cut(s) 406, 983, 1144
TseI GCWGC 6 cut(s) 129, 218, 436, 913, 1065, 1322
Tsp45I GTSAC 3 cut(s) 406, 983, 1144
TspDTI ATGAA 2 cut(s) 639, 1126
TspGWI ACGGA 3 cut(s) 322, 813, 1303
TspRI CASTG 3 cut(s) 187, 973, 1153
VpaK11BI GGWCC 2 cut(s) 897, 933
XagI CCTNNNNNAGG 1 cut(s) 684
XapI RAATTY 1 cut(s) 446
XceI RCATGY 1 cut(s) 1244
XmiI GTMKAC 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 679
XspI CTAG 6 cut(s) 26, 207, 354, 497, 1202, 1362
Zsp2I ATGCAT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.