AT4G10280

Protein of unknown function (DUF861)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012319
Physical Location & Seq
Reverse (-)
6375125 .. 6376142
1018 bp
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UTR
Exon/CDS
Intron
AT4G10280.1

Sequence Viewer

Length: 423 bp
ATGGGTAATCATTTTTTAACGTTATCTCTGCTTCTCGTAACAGTCTGCGTTTGCGTTTCTTTCATCACCACGAAGCTAAACCCTAAAGAAGCAATCGTATCTGTCTCTTCCGATTCTGAGATTCCTACTGAAATTCATGGTGTCAAGATCTTGCGTCAAGCTTCAGATACCAAACTTGCTCAATTAGGCGTTGCCTCTTGGCCCAAGTGGGAAGGTGCTCCAAGCAAGTTTCCATGGGAGTTTAAGAAGACAGAGACAATATATTTCATGGAAGGGAAAGTGAAAGTGAATGTAGACGGATACGACGAAGAAGAAGAAACCTTTGAGATAGGGAAAGGAGATGTTGTTGTTTTCCCTAAAGATATGAAAGTTGTTTGGGAGATAACTGAAGCTGTGAAGAAGCAATATAGCTTAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_3

Protein Analysis

140

Amino Acids

15.79

Weight (kDa)

5.03

Isoelectric Point (pI)

31.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_3 PF05899 60 - 136 1.4e-24 EutQ-like cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10280
fragaria_vesca FvH4_5g20000
malus_domestica MD04G1050000.v1.1 MD06G1049900.v1.1
prunus_persica Prupe.5G060300_v2.0.a1
pyrus_communis pycom04g04400
rosa_chinensis RchiOBHm_Chr7g0204821
rosa_laevigata RLG00000003430 RLG00000017011
rosa_multiflora Rmu_co8494705.1_g000001
rosa_roxburghii Rroxscaffold_3G00252740
rosa_rugosa Rorug07G0086000
rosa_samantha Rh2CG328500 Rh4DG119500 Rh7AG216300 Rh7BG213500 Rh7CG229700 Rh7DG223900
rosa_wichuraiana Rw7G018750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 294
AclI AACGTT 1 cut(s) 20
AcsI RAATTY 1 cut(s) 132
AcuI CTGAAG 2 cut(s) 147, 408
AluBI AGCT 4 cut(s) 76, 161, 392, 411
AluI AGCT 4 cut(s) 76, 161, 392, 411
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 2 cut(s) 109, 248
AoxI GGCC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 132
AspS9I GGNCC 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 58
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 220
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 109, 248
BfuI GTATCC 1 cut(s) 293
BglII AGATCT 1 cut(s) 147
BmgT120I GGNCC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 330, 360
BseDI CCNNGG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 108
BshFI GGCC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 109, 248
BsnI GGCC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 220
Bsp143I GATC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 233
BspANI GGCC 1 cut(s) 202
BspCNI CTCAG 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 233
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 112
BstDEI CTNAG 2 cut(s) 117, 412
BstDSI CCRYGG 1 cut(s) 233
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 2 cut(s) 109, 248
BstMBI GATC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 202
BtgI CCRYGG 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 201
CseI GACGC 1 cut(s) 143
CviAII CATG 3 cut(s) 137, 234, 268
CviJI RGCY 5 cut(s) 76, 161, 202, 392, 411
CviKI_1 RGCY 5 cut(s) 76, 161, 202, 392, 411
DdeI CTNAG 2 cut(s) 117, 412
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 233
Eco57I CTGAAG 2 cut(s) 147, 408
EcoT14I CCWWGG 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 233
FaeI CATG 3 cut(s) 140, 237, 271
FaiI YATR 6 cut(s) 138, 235, 262, 269, 365, 408
FatI CATG 3 cut(s) 136, 233, 267
FblI GTMKAC 1 cut(s) 294
HaeIII GGCC 1 cut(s) 202
HgaI GACGC 1 cut(s) 143
Hin1II CATG 3 cut(s) 140, 237, 271
HindIII AAGCTT 1 cut(s) 159
HinfI GANTC 2 cut(s) 113, 121
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 3 cut(s) 112, 118, 166
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 1 cut(s) 295
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 2 cut(s) 206, 266
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 20
HpyF3I CTNAG 2 cut(s) 117, 412
HpySE526I ACGT 1 cut(s) 20
Hsp92II CATG 3 cut(s) 140, 237, 271
Kzo9I GATC 1 cut(s) 147
LmnI GCTCC 1 cut(s) 223
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 37
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 6 cut(s) 99, 259, 320, 323, 326, 409
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 2 cut(s) 132, 182
MnlI CCTC 2 cut(s) 205, 409
MseI TTAA 2 cut(s) 17, 243
NcoI CCATGG 1 cut(s) 233
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 140, 237, 271
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PfeI GAWTC 2 cut(s) 113, 121
Psp1406I AACGTT 1 cut(s) 20
PspPI GGNCC 1 cut(s) 201
PsuI RGATCY 1 cut(s) 147
SaqAI TTAA 2 cut(s) 17, 243
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 1 cut(s) 201
SduI GDGCHC 1 cut(s) 220
SetI ASST 7 cut(s) 23, 78, 163, 217, 323, 394, 413
Sse9I AATT 2 cut(s) 132, 182
StyI CCWWGG 1 cut(s) 233
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 23
TasI AATT 2 cut(s) 132, 182
TfiI GAWTC 2 cut(s) 113, 121
Tru1I TTAA 2 cut(s) 17, 243
Tru9I TTAA 2 cut(s) 17, 243
TspDTI ATGAA 4 cut(s) 52, 125, 256, 380
TspGWI ACGGA 1 cut(s) 312
XapI RAATTY 1 cut(s) 132
XmiI GTMKAC 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.