AT4G13720

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010856
Physical Location & Seq
Reverse (-)
7966805 .. 7968965
2161 bp
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UTR
Exon/CDS
Intron
AT4G13720.2

Sequence Viewer

Length: 621 bp
ATGGCGGCGGCGGCGGCGAAAGCAGCGGTGGTTTTGCCACGGCCGGTGACGTTTGTGACTGGAAATGCTAAGAAGCTCGAAGAAGTCAAAGCTATCATCGGAAATTCCATTCCTTTCAAGTCTCTCAAACTCGATTTGCCTGAGCTCCAAGGTGAGCCTGAGGATATTTCCAAAGAGAAGGCTCGTTTGGCTGCTCTTCAGGTGGATGGACCAGTGCTTGTGGAAGATACATGTCTCTGTTTCAATGCTTTGAAAGGGCTTCCTGGGCCATACATAAAGTGGTTTCTAGAGAAGCTTGGTCACGAAGGTCTGAACAACTTACTGATGGCCTATGAAGATAAATCAGCTTATGCATTGTGCGCATTCTCTTTCTCGCGTGGTCCAGGTGCTGAACCTCTTACATTTTTGGGGAAAACTCCGGGTAAGATAGTTCCAGCCAGAGGACCAACTGATTTCGGATGGGATCCAGTGTTTCAACCTGATGGATATGACCAAACGTATGCAGAGATGGCAAAGGAAGAAAAGAACAAGATATCACATAGGTACAAGTCTTTAGCATTGGTGAAATCTCACTTTAAGGAGGCTGGCTATGTGTTCCAGACAGATGATGGTACCATTTAA

Protein Analysis

206

Amino Acids

22.48

Weight (kDa)

6.33

Isoelectric Point (pI)

26.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ham1p_like PF01725 29 - 202 1.1e-51 Ham1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13720 AT4G13720
fragaria_vesca FvH4_4g05750
malus_domestica MD13G1230900.v1.1
prunus_persica Prupe.1G060800_v2.0.a1
pyrus_communis pycom13g20350
rosa_chinensis RchiOBHm_Chr4g0396911
rosa_laevigata RLG00000009490 RLG00000027037
rosa_multiflora Rmu_sc0004788.1_g000001 Rmu_sc0008929.1_g000013 Rmu_sc0038532.1_g000001
rosa_roxburghii Rroxscaffold_5G00341830
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075200 Rh4BG071600 Rh4CG079900 Rh4CG295600 Rh4DG069000
rosa_wichuraiana Rw4G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 361
Acc65I GGTACC 1 cut(s) 611
AccB1I GGYRCC 1 cut(s) 611
AccII CGCG 1 cut(s) 376
AciI CCGC 5 cut(s) 5, 8, 11, 14, 26
AclWI GGATC 2 cut(s) 458, 471
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 2 cut(s) 545, 613
AfiI CCNNNNNNNGG 1 cut(s) 440
AflIII ACRYGT 1 cut(s) 230
AgsI TTSAA 4 cut(s) 118, 244, 253, 476
AjnI CCWGG 2 cut(s) 262, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 5 cut(s) 76, 92, 145, 295, 347
AluI AGCT 5 cut(s) 76, 92, 145, 295, 347
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 2 cut(s) 126, 239
AlwI GGATC 2 cut(s) 458, 471
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 3 cut(s) 41, 266, 327
ApeKI GCWGC 2 cut(s) 23, 191
ApoI RAATTY 1 cut(s) 103
Asp718I GGTACC 1 cut(s) 611
AspLEI GCGC 1 cut(s) 362
AspS9I GGNCC 4 cut(s) 209, 266, 380, 443
AsuC2I CCSGG 1 cut(s) 420
AsuHPI GGTGA 3 cut(s) 58, 164, 574
AvaII GGWCC 3 cut(s) 209, 380, 443
AxyI CCTNAGG 1 cut(s) 159
BamHI GGATCC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 35, 178
BccI CCATC 6 cut(s) 200, 319, 453, 476, 502, 602
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 2 cut(s) 264, 384
BcnI CCSGG 1 cut(s) 420
BcoDI GTCTC 2 cut(s) 126, 239
BfaI CTAG 1 cut(s) 287
BisI GCNGC 6 cut(s) 6, 9, 12, 15, 24, 192
BlsI GCNGC 6 cut(s) 7, 10, 13, 16, 25, 193
Bme1390I CCNGG 3 cut(s) 264, 384, 420
Bme18I GGWCC 3 cut(s) 209, 380, 443
BmgT120I GGNCC 4 cut(s) 209, 266, 380, 443
BmiI GGNNCC 2 cut(s) 465, 613
BmrFI CCNGG 3 cut(s) 264, 384, 420
Bpu10I CCTNAGC 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 420
BsaJI CCNNGG 3 cut(s) 38, 148, 263
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 3 cut(s) 64, 212, 467
Bse21I CCTNAGG 1 cut(s) 159
BseBI CCWGG 2 cut(s) 264, 384
BseDI CCNNGG 3 cut(s) 38, 148, 263
BseGI GGATG 2 cut(s) 211, 464
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 2 cut(s) 132, 150
BseNI ACTGG 3 cut(s) 64, 212, 467
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 2 cut(s) 35, 178
Bsh1236I CGCG 1 cut(s) 376
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 3 cut(s) 43, 268, 329
BshNI GGYRCC 1 cut(s) 611
BsiEI CGRYCG 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 2 cut(s) 44, 419
BslI CCNNNNNNNGG 1 cut(s) 440
BsmAI GTCTC 2 cut(s) 126, 239
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 3 cut(s) 43, 268, 329
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 1 cut(s) 463
BspACI CCGC 5 cut(s) 5, 8, 11, 14, 26
BspANI GGCC 3 cut(s) 43, 268, 329
BspCNI CTCAG 2 cut(s) 133, 151
BspFNI CGCG 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 465, 613
BspPI GGATC 2 cut(s) 458, 471
BspQI GCTCTTC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 611
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 3 cut(s) 64, 212, 467
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 3 cut(s) 38, 148, 263
BssMI GATC 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 264, 384
Bst6I CTCTTC 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 586
BstDEI CTNAG 3 cut(s) 69, 141, 159
BstDSI CCRYGG 1 cut(s) 38
BstF5I GGATG 2 cut(s) 211, 464
BstFNI CGCG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 362
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 2 cut(s) 126, 239
BstMBI GATC 1 cut(s) 463
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 8 cut(s) 11, 14, 20, 23, 188, 265, 359, 509
BstNI CCWGG 2 cut(s) 264, 384
BstNSI RCATGY 1 cut(s) 234
BstSCI CCNGG 3 cut(s) 262, 382, 418
BstUI CGCG 1 cut(s) 376
BstV1I GCAGC 2 cut(s) 35, 178
BstX2I RGATCY 1 cut(s) 463
BstYI RGATCY 1 cut(s) 463
BstZI CGGCCG 1 cut(s) 41
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 43, 268, 329
BtgI CCRYGG 1 cut(s) 38
BtsCI GGATG 2 cut(s) 211, 464
BtsIMutI CAGTG 2 cut(s) 219, 474
Cac8I GCNNGC 1 cut(s) 586
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 4 cut(s) 209, 266, 380, 443
Csp6I GTAC 2 cut(s) 544, 612
CviAII CATG 1 cut(s) 231
CviQI GTAC 2 cut(s) 544, 612
DdeI CTNAG 3 cut(s) 69, 141, 159
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 534
Eco47I GGWCC 3 cut(s) 209, 380, 443
Eco52I CGGCCG 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 182
Eco81I CCTNAGG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 262, 382
EcoRV GATATC 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 148
EcoT22I ATGCAT 1 cut(s) 355
EcoT38I GRGCYC 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 1 cut(s) 234
FaiI YATR 9 cut(s) 232, 271, 275, 333, 351, 489, 501, 540, 591
FatI CATG 1 cut(s) 230
Fnu4HI GCNGC 6 cut(s) 6, 9, 12, 15, 24, 192
FokI GGATG 2 cut(s) 218, 471
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 6 cut(s) 6, 9, 12, 15, 24, 192
FspAI RTGCGCAY 1 cut(s) 361
FspBI CTAG 1 cut(s) 287
FspI TGCGCA 1 cut(s) 361
GlaI GCGC 1 cut(s) 361
GluI GCNGC 6 cut(s) 6, 9, 12, 15, 24, 192
HaeIII GGCC 3 cut(s) 43, 268, 329
HapII CCGG 2 cut(s) 44, 419
HhaI GCGC 1 cut(s) 362
Hin1II CATG 1 cut(s) 234
Hin6I GCGC 1 cut(s) 360
HinP1I GCGC 1 cut(s) 360
HindIII AAGCTT 1 cut(s) 293
HpaII CCGG 2 cut(s) 44, 419
HphI GGTGA 3 cut(s) 58, 164, 574
Hpy188I TCNGA 3 cut(s) 101, 312, 458
Hpy188III TCNNGA 3 cut(s) 287, 302, 598
HpyAV CCTTC 2 cut(s) 172, 299
HpyCH4IV ACGT 2 cut(s) 50, 497
HpyCH4V TGCA 2 cut(s) 353, 503
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 14, 20, 23, 188, 265, 359, 509
HpyF3I CTNAG 3 cut(s) 69, 141, 159
HpySE526I ACGT 2 cut(s) 50, 497
Hsp92II CATG 1 cut(s) 234
HspAI GCGC 1 cut(s) 360
KpnI GGTACC 1 cut(s) 615
Kzo9I GATC 1 cut(s) 463
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 150
Lsp1109I GCAGC 2 cut(s) 35, 178
MaeI CTAG 1 cut(s) 287
MaeII ACGT 2 cut(s) 50, 497
MaeIII GTNAC 3 cut(s) 46, 55, 299
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 5 cut(s) 92, 188, 236, 347, 530
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 147
MluCI AATT 1 cut(s) 103
MnlI CCTC 4 cut(s) 154, 405, 434, 574
Mph1103I ATGCAT 1 cut(s) 355
MseI TTAA 2 cut(s) 576, 619
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 2 cut(s) 44, 419
MspR9I CCNGG 3 cut(s) 264, 384, 420
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 2 cut(s) 264, 384
MvnI CGCG 1 cut(s) 376
MwoI GCNNNNNNNGC 8 cut(s) 11, 14, 20, 23, 188, 265, 359, 509
NciI CCSGG 1 cut(s) 420
NdeII GATC 1 cut(s) 463
NlaIII CATG 1 cut(s) 234
NlaIV GGNNCC 2 cut(s) 465, 613
NmuCI GTSAC 3 cut(s) 46, 55, 299
NsbI TGCGCA 1 cut(s) 361
NsiI ATGCAT 1 cut(s) 355
NspI RCATGY 1 cut(s) 234
PciI ACATGT 1 cut(s) 230
PciSI GCTCTTC 1 cut(s) 201
PctI GAATGC 1 cut(s) 362
PkrI GCNGC 6 cut(s) 7, 10, 13, 16, 25, 193
PscI ACATGT 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 147
Psp6I CCWGG 2 cut(s) 262, 382
PspGI CCWGG 2 cut(s) 262, 382
PspN4I GGNNCC 2 cut(s) 465, 613
PspPI GGNCC 4 cut(s) 209, 266, 380, 443
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 2 cut(s) 545, 613
RsaNI GTAC 2 cut(s) 544, 612
SacI GAGCTC 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 576, 619
SatI GCNGC 6 cut(s) 6, 9, 12, 15, 24, 192
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 4 cut(s) 209, 266, 380, 443
ScrFI CCNGG 3 cut(s) 264, 384, 420
SduI GDGCHC 1 cut(s) 147
SinI GGWCC 3 cut(s) 209, 380, 443
Sse9I AATT 1 cut(s) 103
SsiI CCGC 5 cut(s) 5, 8, 11, 14, 26
SspMI CTAG 1 cut(s) 287
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 3 cut(s) 262, 382, 418
StyI CCWWGG 1 cut(s) 148
TaiI ACGT 2 cut(s) 53, 500
TaqI TCGA 2 cut(s) 78, 132
TasI AATT 1 cut(s) 103
TauI GCSGC 4 cut(s) 8, 11, 14, 17
Tru1I TTAA 2 cut(s) 576, 619
Tru9I TTAA 2 cut(s) 576, 619
TscAI CASTG 2 cut(s) 219, 474
TseFI GTSAC 3 cut(s) 46, 55, 299
TseI GCWGC 2 cut(s) 23, 191
Tsp45I GTSAC 3 cut(s) 46, 55, 299
TspDTI ATGAA 1 cut(s) 348
TspRI CASTG 2 cut(s) 219, 474
VpaK11BI GGWCC 3 cut(s) 209, 380, 443
XapI RAATTY 1 cut(s) 103
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 234
XcmI CCANNNNNNNNNTGG 2 cut(s) 276, 605
XspI CTAG 1 cut(s) 287
Zsp2I ATGCAT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.