Rroxscaffold_5G00341830

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010856
Physical Location & Seq
Forward (+)
10444795 .. 10448755
3961 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_5G00341830.1

Sequence Viewer

Length: 570 bp
ATGGCGGCGTTGGGAGCAAGAGGAGGTGGACTGGTGCTGCCAAAGCCGGTGACTTTTGTGACCGGAAACGCCAAGAAGCTTGAAGAAGTGCGCGCCATCTTGGGCACCTCCATTCCCTTCAACTCTCTCAAGCTCGATTTGCCAGAATTGCAAGGGGAGCCTGAGGACATCTCCAAAGAAAAGGCGAGGTTGGCTGCCGTTCAGGTTAATGGTCCTGTGCTTGTGGAAGACACTTGCCTCTGCTTCAATGCCCTCAAGGGTCTACCTGGTCTCAACAATATGTTGATGGCATACGAGGATAAATCTGCATATGCTTTGTGCTCCTTTTCTTTCGCCATTGGGCCCAATGCTGAGCCTATTGCATTTCTGGGTAAAACTCCGGGGAAAATAGTTCCTGCAAGGGGACCAAATGATTTTGGATGGGATCCAATATTTCAACCTGACGGTTACGAGCAAACTTATGCCGAGATGTCAAAGGAAGAAAAGAACAAGATTTCTCATCGCTCTAGAGCTCTTGCACTAGTGAAGTCTCACTTTGCTGAAGCTGCATACACTTTTGAGACCAACTAG

Protein Analysis

189

Amino Acids

20.4

Weight (kDa)

5.77

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ham1p_like PF01725 17 - 176 4.5e-43 Ham1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13720 AT4G13720
fragaria_vesca FvH4_4g05750
malus_domestica MD13G1230900.v1.1
prunus_persica Prupe.1G060800_v2.0.a1
pyrus_communis pycom13g20350
rosa_chinensis RchiOBHm_Chr4g0396911
rosa_laevigata RLG00000009490 RLG00000027037
rosa_multiflora Rmu_sc0004788.1_g000001 Rmu_sc0008929.1_g000013 Rmu_sc0038532.1_g000001
rosa_roxburghii Rroxscaffold_5G00341830
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075200 Rh4BG071600 Rh4CG079900 Rh4CG295600 Rh4DG069000
rosa_wichuraiana Rw4G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 262
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 419, 432
AcuI CTGAAG 1 cut(s) 561
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 4 cut(s) 83, 121, 247, 437
AhlI ACTAGT 1 cut(s) 520
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 4 cut(s) 79, 133, 512, 545
AluI AGCT 4 cut(s) 79, 133, 512, 545
Alw21I GWGCWC 2 cut(s) 323, 514
Alw26I GTCTC 3 cut(s) 275, 534, 554
AlwI GGATC 2 cut(s) 419, 432
AoxI GGCC 1 cut(s) 341
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 3 cut(s) 37, 194, 545
AspLEI GCGC 2 cut(s) 93, 95
AspS9I GGNCC 4 cut(s) 212, 341, 342, 404
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 2 cut(s) 212, 404
AxyI CCTNAGG 1 cut(s) 162
BaeGI GKGCMC 2 cut(s) 107, 345
BamHI GGATCC 1 cut(s) 424
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 345, 514
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 323, 514
BbvI GCAGC 3 cut(s) 24, 181, 532
BccI CCATC 3 cut(s) 104, 280, 414
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 267
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 3 cut(s) 275, 534, 554
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 3 cut(s) 507, 521, 568
BisI GCNGC 4 cut(s) 6, 38, 195, 546
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 4 cut(s) 7, 39, 196, 547
Bme1390I CCNGG 2 cut(s) 267, 381
Bme18I GGWCC 2 cut(s) 212, 404
BmgT120I GGNCC 4 cut(s) 212, 341, 342, 404
BmiI GGNNCC 5 cut(s) 106, 159, 343, 405, 426
BmrFI CCNGG 2 cut(s) 267, 381
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 155, 187
Bpu1102I GCTNAGC 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 113, 239
BpuMI CCSGG 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 275, 554
BsaJI CCNNGG 1 cut(s) 380
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 36
Bse21I CCTNAGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 425
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMII CTCAG 2 cut(s) 153, 342
BseNI ACTGG 1 cut(s) 36
BsePI GCGCGC 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 2 cut(s) 107, 345
BseXI GCAGC 3 cut(s) 24, 181, 532
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 343
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 323, 514
BsiSI CCGG 3 cut(s) 47, 63, 380
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 3 cut(s) 275, 534, 554
BsmFI GGGAC 1 cut(s) 417
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 2 cut(s) 275, 554
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 4 cut(s) 107, 323, 345, 514
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 351
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 2 cut(s) 154, 343
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 5 cut(s) 106, 159, 343, 405, 426
BspPI GGATC 2 cut(s) 419, 432
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 2 cut(s) 275, 554
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 1 cut(s) 36
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 1 cut(s) 380
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 162, 351
BstF5I GGATG 1 cut(s) 425
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 2 cut(s) 93, 95
BstKTI GATC 1 cut(s) 427
BstMAI GTCTC 3 cut(s) 275, 534, 554
BstMBI GATC 1 cut(s) 424
BstMWI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 545
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 265, 379
BstSLI GKGCMC 2 cut(s) 107, 345
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 24, 181, 532
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 1 cut(s) 343
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 2 cut(s) 93, 95
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 4 cut(s) 212, 341, 342, 404
CsiI ACCWGGT 1 cut(s) 265
CviJI RGCY 9 cut(s) 46, 79, 133, 160, 194, 343, 355, 512, 545
CviKI_1 RGCY 9 cut(s) 46, 79, 133, 160, 194, 343, 355, 512, 545
DdeI CTNAG 2 cut(s) 162, 351
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Ecl136II GAGCTC 1 cut(s) 512
Eco24I GRGCYC 2 cut(s) 345, 514
Eco31I GGTCTC 2 cut(s) 275, 554
Eco47I GGWCC 2 cut(s) 212, 404
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 561
Eco81I CCTNAGG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 345, 514
FaiI YATR 6 cut(s) 281, 292, 310, 312, 462, 550
FalI AAGNNNNNCTT 2 cut(s) 518, 550
FaqI GGGAC 1 cut(s) 417
FauNDI CATATG 1 cut(s) 310
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 6, 38, 195, 546
FokI GGATG 1 cut(s) 432
FriOI GRGCYC 2 cut(s) 345, 514
Fsp4HI GCNGC 4 cut(s) 6, 38, 195, 546
FspBI CTAG 3 cut(s) 507, 521, 568
GlaI GCGC 2 cut(s) 92, 94
GluI GCNGC 4 cut(s) 6, 38, 195, 546
HaeIII GGCC 1 cut(s) 343
HapII CCGG 3 cut(s) 47, 63, 380
HhaI GCGC 2 cut(s) 93, 95
Hin6I GCGC 2 cut(s) 91, 93
HinP1I GCGC 2 cut(s) 91, 93
HindIII AAGCTT 1 cut(s) 77
HpaII CCGG 3 cut(s) 47, 63, 380
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 29, 263
Hpy188III TCNNGA 1 cut(s) 507
Hpy8I GTNNAC 2 cut(s) 29, 263
HpyAV CCTTC 1 cut(s) 127
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4V TGCA 6 cut(s) 151, 308, 362, 398, 518, 548
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 545
HpyF3I CTNAG 2 cut(s) 162, 351
HspAI GCGC 2 cut(s) 91, 93
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 3 cut(s) 14, 157, 326
Lsp1109I GCAGC 3 cut(s) 24, 181, 532
MabI ACCWGGT 1 cut(s) 265
MaeI CTAG 3 cut(s) 507, 521, 568
MaeIII GTNAC 3 cut(s) 49, 58, 446
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 3 cut(s) 95, 239, 491
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 4 cut(s) 107, 323, 345, 514
MluCI AATT 1 cut(s) 146
MnlI CCTC 8 cut(s) 14, 17, 118, 157, 180, 248, 263, 289
MseI TTAA 1 cut(s) 207
MspI CCGG 3 cut(s) 47, 63, 380
MspR9I CCNGG 2 cut(s) 267, 381
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 545
NciI CCSGG 1 cut(s) 381
NdeI CATATG 1 cut(s) 310
NdeII GATC 1 cut(s) 424
NlaIV GGNNCC 5 cut(s) 106, 159, 343, 405, 426
NmeAIII GCCGAG 1 cut(s) 490
NmuCI GTSAC 2 cut(s) 49, 58
PauI GCGCGC 1 cut(s) 91
PkrI GCNGC 4 cut(s) 7, 39, 196, 547
Psp124BI GAGCTC 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 106, 159, 343, 405, 426
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 4 cut(s) 212, 341, 342, 404
PsuI RGATCY 1 cut(s) 424
PteI GCGCGC 1 cut(s) 91
SacI GAGCTC 1 cut(s) 514
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 6, 38, 195, 546
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 4 cut(s) 212, 341, 342, 404
ScrFI CCNGG 2 cut(s) 267, 381
SduI GDGCHC 4 cut(s) 107, 323, 345, 514
SexAI ACCWGGT 1 cut(s) 265
SinI GGWCC 2 cut(s) 212, 404
SmlI CTYRAG 2 cut(s) 128, 254
SmoI CTYRAG 2 cut(s) 128, 254
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 1 cut(s) 146
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 432
SspMI CTAG 3 cut(s) 507, 521, 568
SstI GAGCTC 1 cut(s) 514
StyD4I CCNGG 2 cut(s) 265, 379
TaaI ACNGT 1 cut(s) 446
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 146
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 2 cut(s) 49, 58
TseI GCWGC 3 cut(s) 37, 194, 545
Tsp45I GTSAC 2 cut(s) 49, 58
VpaK11BI GGWCC 2 cut(s) 212, 404
XbaI TCTAGA 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 3 cut(s) 507, 521, 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.