Rw4G006030

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010856
Physical Location & Seq
Forward (+)
12199265 .. 12203330
4066 bp
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UTR
Exon/CDS
Intron
Rw4G006030.1

Sequence Viewer

Length: 612 bp
ATGGCGGCGTTGGGAGCAAGAGGAGGTGGACTGGTGCTGCCAAAGCCGGTGACTTTTGTGACCGGAAACGCCAAGAAGCTCGAAGAAGTGCGCGCCATCTTGGGCACCTCCATTCCCTTCAACTCTCTCAAGCTCGATTTGCCAGAATTGCAAGGGGAGCCTGAGGACATCTCCAAAGAAAAGGCGAGGTTGGCTGCCGTTCAGGTTAATGGTCCTGTGCTTGTGGAAGACACTTGCCTCTGCTTCAATGCCCTCAAGGGTCTACCTGGGCCTTACATCAAGTGGTTCCTGCAGAAGATTGGACATGAAGGTCTCAACAATATGTTGATGGCATATGAGGATAAATCTGCATATGCTTTGTGCTCCTTTTCTTTTGCCATTGGGCCCAATGCTGAGCCTATTGCATTTCTGGGAAAAACTCCGGGGAAAATAGTTCCTGCAAGGGGACCAAATGATTTTGGATGGGATCCAATATTTCAGCCTGATGGTTACGAGCAAACTTATGCCGAGATGTCAAAGGAAGAAAAGAACAAGATTTCTCATCGCTCTAGAGCTCTTGCACTAGTGAAGTCTCACTTTGCTGAAGCTGCATACACTTTTGAGACGAACTAG

Protein Analysis

203

Amino Acids

22.1

Weight (kDa)

6.32

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ham1p_like PF01725 17 - 190 1.6e-53 Ham1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13720 AT4G13720
fragaria_vesca FvH4_4g05750
malus_domestica MD13G1230900.v1.1
prunus_persica Prupe.1G060800_v2.0.a1
pyrus_communis pycom13g20350
rosa_chinensis RchiOBHm_Chr4g0396911
rosa_laevigata RLG00000009490 RLG00000027037
rosa_multiflora Rmu_sc0004788.1_g000001 Rmu_sc0008929.1_g000013 Rmu_sc0038532.1_g000001
rosa_roxburghii Rroxscaffold_5G00341830
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075200 Rh4BG071600 Rh4CG079900 Rh4CG295600 Rh4DG069000
rosa_wichuraiana Rw4G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 262
AccII CGCG 1 cut(s) 93
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 461, 474
AcuI CTGAAG 1 cut(s) 603
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 2 cut(s) 121, 247
AhlI ACTAGT 1 cut(s) 562
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 4 cut(s) 79, 133, 554, 587
AluI AGCT 4 cut(s) 79, 133, 554, 587
Alw21I GWGCWC 2 cut(s) 365, 556
Alw26I GTCTC 3 cut(s) 317, 576, 596
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 2 cut(s) 269, 383
ApaI GGGCCC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 37, 194, 587
AspLEI GCGC 2 cut(s) 93, 95
AspS9I GGNCC 5 cut(s) 212, 269, 383, 384, 446
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 2 cut(s) 212, 446
AxyI CCTNAGG 1 cut(s) 162
BaeGI GKGCMC 2 cut(s) 107, 387
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 387, 556
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 2 cut(s) 365, 556
BbvI GCAGC 3 cut(s) 24, 181, 574
BccI CCATC 4 cut(s) 104, 322, 456, 479
BceAI ACGGC 1 cut(s) 182
BciT130I CCWGG 1 cut(s) 267
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 317, 576, 596
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 3 cut(s) 549, 563, 610
BfmI CTRYAG 1 cut(s) 290
BisI GCNGC 4 cut(s) 6, 38, 195, 588
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 4 cut(s) 7, 39, 196, 589
Bme1390I CCNGG 2 cut(s) 267, 423
Bme18I GGWCC 2 cut(s) 212, 446
BmgT120I GGNCC 5 cut(s) 212, 269, 383, 384, 446
BmiI GGNNCC 6 cut(s) 106, 159, 287, 385, 447, 468
BmrFI CCNGG 2 cut(s) 267, 423
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 155, 187
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 113, 239
BpuMI CCSGG 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 266, 422
BsaWI WCCGGW 1 cut(s) 62
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 36
Bse21I CCTNAGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 266, 422
BseGI GGATG 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 2 cut(s) 153, 384
BseNI ACTGG 1 cut(s) 36
BsePI GCGCGC 1 cut(s) 91
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 2 cut(s) 107, 387
BseXI GCAGC 3 cut(s) 24, 181, 574
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 2 cut(s) 271, 385
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 365, 556
BsiSI CCGG 3 cut(s) 47, 63, 422
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 3 cut(s) 317, 576, 596
BsmBI CGTCTC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 2 cut(s) 271, 385
Bso31I GGTCTC 1 cut(s) 317
Bsp120I GGGCCC 1 cut(s) 383
Bsp1286I GDGCHC 4 cut(s) 107, 365, 387, 556
Bsp143I GATC 1 cut(s) 466
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 271, 385
BspCNI CTCAG 2 cut(s) 154, 385
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 6 cut(s) 106, 159, 287, 385, 447, 468
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 2 cut(s) 461, 474
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 1 cut(s) 36
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 266, 422
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 2 cut(s) 162, 393
BstF5I GGATG 1 cut(s) 467
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 2 cut(s) 93, 95
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 3 cut(s) 317, 576, 596
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 587
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 265, 421
BstSFI CTRYAG 1 cut(s) 290
BstSLI GKGCMC 2 cut(s) 107, 387
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 3 cut(s) 24, 181, 574
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 2 cut(s) 271, 385
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 1 cut(s) 467
Cac8I GCNNGC 1 cut(s) 93
CfoI GCGC 2 cut(s) 93, 95
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 5 cut(s) 212, 269, 383, 384, 446
CviAII CATG 1 cut(s) 305
DdeI CTNAG 2 cut(s) 162, 393
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DrdI GACNNNNNNGTC 1 cut(s) 309
DseDI GACNNNNNNGTC 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 554
Eco24I GRGCYC 2 cut(s) 387, 556
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 212, 446
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 603
Eco81I CCTNAGG 1 cut(s) 162
EcoICRI GAGCTC 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 387, 556
Esp3I CGTCTC 1 cut(s) 596
FaeI CATG 1 cut(s) 308
FaiI YATR 8 cut(s) 306, 323, 334, 336, 352, 354, 504, 592
FalI AAGNNNNNCTT 2 cut(s) 560, 592
FaqI GGGAC 1 cut(s) 459
FatI CATG 1 cut(s) 304
FauNDI CATATG 2 cut(s) 334, 352
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 6, 38, 195, 588
FokI GGATG 1 cut(s) 474
FriOI GRGCYC 2 cut(s) 387, 556
Fsp4HI GCNGC 4 cut(s) 6, 38, 195, 588
FspBI CTAG 3 cut(s) 549, 563, 610
GlaI GCGC 2 cut(s) 92, 94
GluI GCNGC 4 cut(s) 6, 38, 195, 588
HaeIII GGCC 2 cut(s) 271, 385
HapII CCGG 3 cut(s) 47, 63, 422
HhaI GCGC 2 cut(s) 93, 95
Hin1II CATG 1 cut(s) 308
Hin6I GCGC 2 cut(s) 91, 93
HinP1I GCGC 2 cut(s) 91, 93
HpaII CCGG 3 cut(s) 47, 63, 422
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 29, 263
Hpy188III TCNNGA 1 cut(s) 549
Hpy8I GTNNAC 2 cut(s) 29, 263
HpyAV CCTTC 2 cut(s) 127, 302
HpyCH4V TGCA 7 cut(s) 151, 292, 350, 404, 440, 560, 590
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 587
HpyF3I CTNAG 2 cut(s) 162, 393
Hsp92II CATG 1 cut(s) 308
HspAI GCGC 2 cut(s) 91, 93
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 3 cut(s) 14, 157, 368
Lsp1109I GCAGC 3 cut(s) 24, 181, 574
MaeI CTAG 3 cut(s) 549, 563, 610
MaeIII GTNAC 3 cut(s) 49, 58, 488
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 4 cut(s) 95, 239, 307, 533
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 4 cut(s) 107, 365, 387, 556
MluCI AATT 1 cut(s) 146
MnlI CCTC 8 cut(s) 14, 17, 118, 157, 180, 248, 263, 331
MseI TTAA 1 cut(s) 207
MspI CCGG 3 cut(s) 47, 63, 422
MspR9I CCNGG 2 cut(s) 267, 423
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 7 cut(s) 14, 43, 139, 148, 157, 191, 587
NciI CCSGG 1 cut(s) 423
NdeI CATATG 2 cut(s) 334, 352
NdeII GATC 1 cut(s) 466
NlaIII CATG 1 cut(s) 308
NlaIV GGNNCC 6 cut(s) 106, 159, 287, 385, 447, 468
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 2 cut(s) 49, 58
PauI GCGCGC 1 cut(s) 91
PkrI GCNGC 4 cut(s) 7, 39, 196, 589
Psp124BI GAGCTC 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 6 cut(s) 106, 159, 287, 385, 447, 468
PspOMI GGGCCC 1 cut(s) 383
PspPI GGNCC 5 cut(s) 212, 269, 383, 384, 446
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 466
PteI GCGCGC 1 cut(s) 91
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 6, 38, 195, 588
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 5 cut(s) 212, 269, 383, 384, 446
ScrFI CCNGG 2 cut(s) 267, 423
SduI GDGCHC 4 cut(s) 107, 365, 387, 556
SfcI CTRYAG 1 cut(s) 290
SinI GGWCC 2 cut(s) 212, 446
SmlI CTYRAG 2 cut(s) 128, 254
SmoI CTYRAG 2 cut(s) 128, 254
SpeI ACTAGT 1 cut(s) 562
Sse9I AATT 1 cut(s) 146
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 474
SspMI CTAG 3 cut(s) 549, 563, 610
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 2 cut(s) 265, 421
TaqI TCGA 2 cut(s) 81, 135
TasI AATT 1 cut(s) 146
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 2 cut(s) 49, 58
TseI GCWGC 3 cut(s) 37, 194, 587
Tsp45I GTSAC 2 cut(s) 49, 58
TspDTI ATGAA 1 cut(s) 321
VpaK11BI GGWCC 2 cut(s) 212, 446
XbaI TCTAGA 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 3 cut(s) 549, 563, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.