pycom13g20350

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010856
Physical Location & Seq
Forward (+)
16408248 .. 16412286
4039 bp
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UTR
Exon/CDS
Intron
pycom13g20350.1

Sequence Viewer

Length: 618 bp
ATGGCGGCTGTGGGAGCAAGAGGAAGTGGGTTGGTCCTGCCAAAGCCGGTGACTTTTGTGACTGGCAACGCTAAGAAGCTCGAAGAAGTTCGTGCCATCTTGGGCACCTCCATTCCCTTCAACTCTCTCAAGCTTGACTTGCCGGAATTGCAAGGCGAACCAGAGGACATATCCAAGGAAAAGGCTCGGTTGGCTGCCATTCAGGTAAATGGTCCTGTGCTCGTGGAAGACACTTGCCTCTGCTTCAATGCCCTCAAGGGTCTACCTGGACCTTACATTAAGTGGTTCCTGCAGAAGCTTGGACATGAAGGTCTCAACAATTTGTTGATGGCATATGAGGATAAATCAGCCTATGCATTGTGTTCCTTCTCCTTTACCACTGGGCCCAGTGTTGAGCCTATCACATTTCTGGGTAAAACTCCGGGGAGAATAGTTCCAGCAAGGGGACCAACTGATTTCGGATGGGATCCAGTCTTCCAACCTGATGGTTACGAGCAGACTTATGCCGAGATGCCAAAGGAAGAAAAGAACAAGATTTCTCATCGCTTTAGAGCTCTTGCACTGGTGAAGTCTCACTTTGCTGAAGCTAGGTACACTTTCGAGACAAACTCTGTGTAA

Protein Analysis

206

Amino Acids

22.5

Weight (kDa)

6.84

Isoelectric Point (pI)

29.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ham1p_like PF01725 17 - 190 1.7e-52 Ham1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13720 AT4G13720
fragaria_vesca FvH4_4g05750
malus_domestica MD13G1230900.v1.1
prunus_persica Prupe.1G060800_v2.0.a1
pyrus_communis pycom13g20350
rosa_chinensis RchiOBHm_Chr4g0396911
rosa_laevigata RLG00000009490 RLG00000027037
rosa_multiflora Rmu_sc0004788.1_g000001 Rmu_sc0008929.1_g000013 Rmu_sc0038532.1_g000001
rosa_roxburghii Rroxscaffold_5G00341830
rosa_rugosa Rorug03G0346100
rosa_samantha Rh4AG075200 Rh4BG071600 Rh4CG079900 Rh4CG295600 Rh4DG069000
rosa_wichuraiana Rw4G006030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 1 cut(s) 262
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 461, 474
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 1 cut(s) 593
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 2 cut(s) 121, 247
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 5 cut(s) 79, 133, 298, 554, 587
AluI AGCT 5 cut(s) 79, 133, 298, 554, 587
Alw21I GWGCWC 2 cut(s) 222, 556
Alw26I GTCTC 3 cut(s) 317, 576, 596
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 1 cut(s) 383
ApaI GGGCCC 1 cut(s) 387
ApeKI GCWGC 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 87
AspS9I GGNCC 6 cut(s) 34, 212, 269, 383, 384, 446
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 2 cut(s) 61, 577
AvaII GGWCC 4 cut(s) 34, 212, 269, 446
BaeGI GKGCMC 2 cut(s) 107, 387
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 2 cut(s) 387, 556
BauI CACGAG 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 234, 466
Bbv12I GWGCWC 2 cut(s) 222, 556
BbvI GCAGC 1 cut(s) 181
BccI CCATC 4 cut(s) 104, 322, 456, 479
BciT130I CCWGG 1 cut(s) 267
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 3 cut(s) 317, 576, 596
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 290
BisI GCNGC 2 cut(s) 6, 195
BlsI GCNGC 2 cut(s) 7, 196
Bme1390I CCNGG 2 cut(s) 267, 423
Bme18I GGWCC 4 cut(s) 34, 212, 269, 446
BmgT120I GGNCC 6 cut(s) 34, 212, 269, 383, 384, 446
BmiI GGNNCC 5 cut(s) 106, 287, 385, 447, 468
BmrFI CCNGG 2 cut(s) 267, 423
BmrI ACTGGG 2 cut(s) 381, 390
BmsI GCATC 1 cut(s) 501
BmuI ACTGGG 2 cut(s) 381, 390
BpiI GAAGAC 2 cut(s) 234, 466
BplI GAGNNNNNCTC 1 cut(s) 593
BpuEI CTTGAG 2 cut(s) 113, 239
BpuMI CCSGG 1 cut(s) 423
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 174, 422
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse118I RCCGGY 1 cut(s) 46
Bse1I ACTGG 5 cut(s) 67, 385, 387, 470, 567
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 174, 422
BseGI GGATG 1 cut(s) 467
BseLI CCNNNNNNNGG 1 cut(s) 443
BseNI ACTGG 5 cut(s) 67, 385, 387, 470, 567
BseSI GKGCMC 2 cut(s) 107, 387
BseXI GCAGC 1 cut(s) 181
BshFI GGCC 1 cut(s) 385
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 222, 556
BsiSI CCGG 3 cut(s) 47, 143, 422
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 3 cut(s) 317, 576, 596
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 1 cut(s) 385
Bso31I GGTCTC 1 cut(s) 317
Bsp120I GGGCCC 1 cut(s) 383
Bsp1286I GDGCHC 4 cut(s) 107, 222, 387, 556
Bsp143I GATC 1 cut(s) 466
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 385
BspLI GGNNCC 5 cut(s) 106, 287, 385, 447, 468
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 2 cut(s) 461, 474
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 46
BsrI ACTGG 5 cut(s) 67, 385, 387, 470, 567
BssAI RCCGGY 1 cut(s) 46
BssECI CCNNGG 2 cut(s) 174, 422
BssMI GATC 1 cut(s) 466
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 174
Bst2BI CACGAG 1 cut(s) 221
Bst2UI CCWGG 1 cut(s) 267
BstDEI CTNAG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 467
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 3 cut(s) 317, 576, 596
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 4 cut(s) 14, 139, 148, 191
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 265, 421
BstSFI CTRYAG 1 cut(s) 290
BstSLI GKGCMC 2 cut(s) 107, 387
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 2 cut(s) 234, 466
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 485
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 1 cut(s) 385
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 3 cut(s) 378, 394, 560
Cfr10I RCCGGY 1 cut(s) 46
Cfr13I GGNCC 6 cut(s) 34, 212, 269, 383, 384, 446
Csp6I GTAC 1 cut(s) 592
CviAII CATG 1 cut(s) 305
CviQI GTAC 1 cut(s) 592
DdeI CTNAG 1 cut(s) 72
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
DrdI GACNNNNNNGTC 1 cut(s) 309
DseDI GACNNNNNNGTC 1 cut(s) 309
Ecl136II GAGCTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 174
Eco24I GRGCYC 2 cut(s) 387, 556
Eco31I GGTCTC 1 cut(s) 317
Eco47I GGWCC 4 cut(s) 34, 212, 269, 446
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 554
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 174
EcoT22I ATGCAT 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 387, 556
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 1 cut(s) 308
FaiI YATR 6 cut(s) 170, 306, 334, 336, 354, 504
FalI AAGNNNNNCTT 4 cut(s) 122, 154, 560, 592
FaqI GGGAC 1 cut(s) 459
FatI CATG 1 cut(s) 304
FauNDI CATATG 1 cut(s) 334
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 6, 195
FokI GGATG 1 cut(s) 474
FriOI GRGCYC 2 cut(s) 387, 556
Fsp4HI GCNGC 2 cut(s) 6, 195
FspBI CTAG 1 cut(s) 588
GluI GCNGC 2 cut(s) 6, 195
HaeIII GGCC 1 cut(s) 385
HapII CCGG 3 cut(s) 47, 143, 422
Hin1II CATG 1 cut(s) 308
HindIII AAGCTT 2 cut(s) 131, 296
HpaII CCGG 3 cut(s) 47, 143, 422
HphI GGTGA 2 cut(s) 61, 577
Hpy166II GTNNAC 2 cut(s) 263, 594
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 1 cut(s) 601
Hpy8I GTNNAC 2 cut(s) 263, 594
HpyAV CCTTC 3 cut(s) 127, 302, 376
HpyCH4V TGCA 4 cut(s) 151, 292, 356, 560
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 139, 148, 191
HpyF3I CTNAG 1 cut(s) 72
Hsp92II CATG 1 cut(s) 308
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 1 cut(s) 501
MaeI CTAG 1 cut(s) 588
MaeIII GTNAC 3 cut(s) 49, 58, 488
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 4 cut(s) 95, 239, 466, 533
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 4 cut(s) 107, 222, 387, 556
MluCI AATT 2 cut(s) 146, 319
MmeI TCCRAC 1 cut(s) 502
MnlI CCTC 6 cut(s) 14, 118, 157, 248, 263, 331
Mph1103I ATGCAT 1 cut(s) 358
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 279
MspI CCGG 3 cut(s) 47, 143, 422
MspR9I CCNGG 2 cut(s) 267, 423
MvaI CCWGG 1 cut(s) 267
MwoI GCNNNNNNNGC 4 cut(s) 14, 139, 148, 191
NciI CCSGG 1 cut(s) 423
NdeI CATATG 1 cut(s) 334
NdeII GATC 1 cut(s) 466
NlaIII CATG 1 cut(s) 308
NlaIV GGNNCC 5 cut(s) 106, 287, 385, 447, 468
NmeAIII GCCGAG 1 cut(s) 532
NmuCI GTSAC 2 cut(s) 49, 58
NsiI ATGCAT 1 cut(s) 358
PdmI GAANNNNTTC 1 cut(s) 87
PkrI GCNGC 2 cut(s) 7, 196
Psp124BI GAGCTC 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 106, 287, 385, 447, 468
PspOMI GGGCCC 1 cut(s) 383
PspPI GGNCC 6 cut(s) 34, 212, 269, 383, 384, 446
PstI CTGCAG 1 cut(s) 294
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 1 cut(s) 593
RsaNI GTAC 1 cut(s) 592
SacI GAGCTC 1 cut(s) 556
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 2 cut(s) 6, 195
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 6 cut(s) 34, 212, 269, 383, 384, 446
ScrFI CCNGG 2 cut(s) 267, 423
SduI GDGCHC 4 cut(s) 107, 222, 387, 556
SfaNI GCATC 1 cut(s) 501
SfcI CTRYAG 1 cut(s) 290
SinI GGWCC 4 cut(s) 34, 212, 269, 446
SmlI CTYRAG 2 cut(s) 128, 254
SmoI CTYRAG 2 cut(s) 128, 254
Sse9I AATT 2 cut(s) 146, 319
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 2 cut(s) 265, 421
StyI CCWWGG 1 cut(s) 174
TaqI TCGA 2 cut(s) 81, 600
TasI AATT 2 cut(s) 146, 319
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 3 cut(s) 385, 394, 567
TseFI GTSAC 2 cut(s) 49, 58
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 2 cut(s) 49, 58
TspDTI ATGAA 1 cut(s) 321
TspRI CASTG 3 cut(s) 385, 394, 567
VpaK11BI GGWCC 4 cut(s) 34, 212, 269, 446
XmiI GTMKAC 1 cut(s) 262
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 1 cut(s) 588
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.