AT4G13870

Werner Syndrome-like exonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003549
Physical Location & Seq
Reverse (-)
8023237 .. 8025592
2356 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13870.2

Sequence Viewer

Length: 858 bp
ATGTCATCGTCAAATTGGATCGACGACGCTTTTACAGAGGAAGAGCTTCTCGCTATCGACGCCATCGAAGCTTCCTACAATTTCTCCCGTTCTTCTTCTTCTTCTTCCTCTGCTGCTCCGACCGTACAAGCTACAACCTCCGTCCATGGCCACGAGGAGGATCCAAATCAAATCCCCAATAATATCCGTCGCCAATTGCCTCGTTCCATCACTTCTTCTACATCTTATAAACGATTTCCTCTCTCCCGTTGCCGAGCTAGGAATTTTCCAGCAATGAGGTTTGGTGGTAGGATTTTGTATAGCAAGACTGCTACTGAGGTTGATAAGCGAGCAATGCAGCTTATTAAAGTTCTTGATACCAAGAGAGATGAATCTGGAATAGCTTTTGTTGGCTTGGATATTGAGTGGAGACCAAGTTTTAGAAAAGGTGTTCTCCCGGGGAAGGTTGCGACTGTCCAGATATGTGTAGATAGTAATTATTGTGATGTTATGCATATTTTTCATTCTGGTATCCCTCAAAGTCTCCAACATCTTATTGAAGATTCAACACTTGTAAAGGTAGGTATTGGAATTGATGGTGACTCTGTGAAGCTTTTCCATGACTATGGAGTTAGTATCAAAGATGTTGAGGATCTTTCAGATTTAGCCAACCAAAAAATTGGTGGAGATAAAAAATGGGGCCTTGCCTCACTAACTGAGACACTTGTTTGCAAAGAGCTCCTGAAGCCAAACAGAATCAGGCTTGGGAACTGGGAGTTTTATCCTCTGTCAAAGCAGCAGTTACAATACGCAGCAACGGATGCTTATGCTTCATGGCATCTTTACAAGGACCTTCCTGATGCTGTCAGTGGCTCATAA

Protein Analysis

285

Amino Acids

31.75

Weight (kDa)

6.39

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 129 - 279 4.6e-18 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 228
AclWI GGATC 4 cut(s) 26, 155, 168, 639
AcoI YGGCCR 1 cut(s) 148
AcsI RAATTY 1 cut(s) 262
AcuI CTGAAG 1 cut(s) 743
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 157, 442
AgsI TTSAA 2 cut(s) 539, 546
AluBI AGCT 8 cut(s) 46, 71, 131, 257, 340, 383, 592, 718
AluI AGCT 8 cut(s) 46, 71, 131, 257, 340, 383, 592, 718
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 3 cut(s) 403, 527, 692
AlwI GGATC 4 cut(s) 26, 155, 168, 639
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 2 cut(s) 148, 679
ApeKI GCWGC 4 cut(s) 113, 337, 775, 791
ApoI RAATTY 1 cut(s) 262
Asp700I GAANNNNTTC 2 cut(s) 45, 593
AspS9I GGNCC 2 cut(s) 679, 829
AsuC2I CCSGG 2 cut(s) 437, 438
AsuHPI GGTGA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 1 cut(s) 829
BalI TGGCCA 1 cut(s) 150
BamHI GGATCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 720
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 4 cut(s) 100, 349, 787, 803
BccI CCATC 3 cut(s) 71, 215, 569
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 2 cut(s) 437, 438
BcoDI GTCTC 3 cut(s) 403, 527, 692
BfaI CTAG 1 cut(s) 258
BfuI GTATCC 1 cut(s) 521
BisI GCNGC 4 cut(s) 114, 338, 776, 792
BlsI GCNGC 4 cut(s) 115, 339, 777, 793
Bme1390I CCNGG 2 cut(s) 437, 438
Bme18I GGWCC 1 cut(s) 829
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 2 cut(s) 679, 829
BmiI GGNNCC 2 cut(s) 162, 680
BmrFI CCNGG 2 cut(s) 437, 438
BmrI ACTGGG 1 cut(s) 760
BmsI GCATC 3 cut(s) 790, 826, 829
BmuI ACTGGG 1 cut(s) 760
BpuMI CCSGG 2 cut(s) 437, 438
BsaBI GATNNNNATC 1 cut(s) 165
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 403
BsaJI CCNNGG 3 cut(s) 145, 436, 437
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 400, 430
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 442
Bse1I ACTGG 1 cut(s) 755
Bse3DI GCAATG 2 cut(s) 279, 339
Bse8I GATNNNNATC 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 145, 436, 437
BseGI GGATG 1 cut(s) 805
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 2 cut(s) 157, 442
BseMI GCAATG 2 cut(s) 279, 339
BseMII CTCAG 2 cut(s) 306, 687
BseNI ACTGG 1 cut(s) 755
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 4 cut(s) 100, 349, 787, 803
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 2 cut(s) 150, 681
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 157, 442
BsmAI GTCTC 3 cut(s) 403, 527, 692
BsnI GGCC 2 cut(s) 150, 681
Bso31I GGTCTC 1 cut(s) 403
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 1 cut(s) 720
Bsp143I GATC 3 cut(s) 18, 160, 631
Bsp19I CCATGG 1 cut(s) 145
BspANI GGCC 2 cut(s) 150, 681
BspCNI CTCAG 2 cut(s) 307, 688
BspLI GGNNCC 2 cut(s) 162, 680
BspPI GGATC 4 cut(s) 26, 155, 168, 639
BspQI GCTCTTC 1 cut(s) 36
BspTNI GGTCTC 1 cut(s) 403
BsrDI GCAATG 2 cut(s) 279, 339
BsrI ACTGG 1 cut(s) 755
BssECI CCNNGG 3 cut(s) 145, 436, 437
BssMI GATC 3 cut(s) 18, 160, 631
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 152
BssT1I CCWWGG 1 cut(s) 145
Bst2BI CACGAG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 124, 454
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 315, 696
BstDSI CCRYGG 1 cut(s) 145
BstF5I GGATG 1 cut(s) 805
BstKTI GATC 3 cut(s) 21, 163, 634
BstMAI GTCTC 3 cut(s) 403, 527, 692
BstMBI GATC 3 cut(s) 18, 160, 631
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 5 cut(s) 59, 68, 334, 724, 800
BstSCI CCNGG 2 cut(s) 435, 436
BstV1I GCAGC 4 cut(s) 100, 349, 787, 803
BstX2I RGATCY 2 cut(s) 160, 631
BstXI CCANNNNNNTGG 2 cut(s) 605, 659
BstYI RGATCY 2 cut(s) 160, 631
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 2 cut(s) 150, 681
BtgI CCRYGG 1 cut(s) 145
BtsCI GGATG 1 cut(s) 805
BtsIMutI CAGTG 1 cut(s) 853
Cac8I GCNNGC 1 cut(s) 330
Cfr13I GGNCC 2 cut(s) 679, 829
Cfr9I CCCGGG 1 cut(s) 436
CseI GACGC 2 cut(s) 35, 68
Csp6I GTAC 1 cut(s) 125
CviAII CATG 3 cut(s) 146, 599, 813
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 2 cut(s) 315, 696
DpnI GATC 3 cut(s) 20, 162, 633
DpnII GATC 3 cut(s) 18, 160, 631
EaeI YGGCCR 1 cut(s) 148
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 718
Eco130I CCWWGG 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 720
Eco31I GGTCTC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 829
Eco53kI GAGCTC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 718
EcoO109I RGGNCCY 2 cut(s) 679, 829
EcoT14I CCWWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 720
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 149, 602, 816
FatI CATG 3 cut(s) 145, 598, 812
Fnu4HI GCNGC 4 cut(s) 114, 338, 776, 792
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 1 cut(s) 720
Fsp4HI GCNGC 4 cut(s) 114, 338, 776, 792
FspBI CTAG 1 cut(s) 258
GluI GCNGC 4 cut(s) 114, 338, 776, 792
HaeIII GGCC 2 cut(s) 150, 681
HapII CCGG 1 cut(s) 437
HgaI GACGC 2 cut(s) 35, 68
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 149, 602, 816
HindIII AAGCTT 2 cut(s) 69, 590
HinfI GANTC 4 cut(s) 371, 542, 581, 735
HpaII CCGG 1 cut(s) 437
HphI GGTGA 1 cut(s) 590
Hpy188I TCNGA 2 cut(s) 120, 640
Hpy188III TCNNGA 5 cut(s) 353, 375, 457, 721, 836
Hpy99I CGWCG 4 cut(s) 26, 29, 62, 192
HpyAV CCTTC 2 cut(s) 436, 842
HpyCH4III ACNGT 2 cut(s) 124, 454
HpyCH4V TGCA 3 cut(s) 337, 493, 711
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 68, 334, 724, 800
HpyF3I CTNAG 2 cut(s) 315, 696
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 149, 602, 816
Kzo9I GATC 3 cut(s) 18, 160, 631
LguI GCTCTTC 1 cut(s) 36
LmnI GCTCC 2 cut(s) 121, 723
LpnPI CCDG 9 cut(s) 282, 360, 450, 470, 492, 724, 734, 736, 849
Lsp1109I GCAGC 4 cut(s) 100, 349, 787, 803
LweI GCATC 3 cut(s) 790, 826, 829
MaeI CTAG 1 cut(s) 258
MaeIII GTNAC 2 cut(s) 578, 780
MalI GATC 3 cut(s) 20, 162, 633
MboI GATC 3 cut(s) 18, 160, 631
MboII GAAGA 8 cut(s) 53, 84, 87, 90, 93, 96, 207, 551
MfeI CAATTG 1 cut(s) 194
MflI RGATCY 2 cut(s) 160, 631
MhlI GDGCHC 1 cut(s) 720
MlsI TGGCCA 1 cut(s) 150
MluCI AATT 7 cut(s) 13, 79, 194, 262, 475, 570, 657
MluNI TGGCCA 1 cut(s) 150
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 2 cut(s) 143, 550
Mox20I TGGCCA 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 495
MroXI GAANNNNTTC 2 cut(s) 45, 593
MscI TGGCCA 1 cut(s) 150
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 603
Msp20I TGGCCA 1 cut(s) 150
MspI CCGG 1 cut(s) 437
MspR9I CCNGG 2 cut(s) 437, 438
MunI CAATTG 1 cut(s) 194
MwoI GCNNNNNNNGC 5 cut(s) 59, 68, 334, 724, 800
NciI CCSGG 2 cut(s) 437, 438
NcoI CCATGG 1 cut(s) 145
NdeII GATC 3 cut(s) 18, 160, 631
NlaIII CATG 3 cut(s) 149, 602, 816
NlaIV GGNNCC 2 cut(s) 162, 680
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 1 cut(s) 578
NsiI ATGCAT 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 36
PcsI WCGNNNNNNNCGW 1 cut(s) 63
PdmI GAANNNNTTC 2 cut(s) 45, 593
PfeI GAWTC 3 cut(s) 371, 542, 735
PkrI GCNGC 4 cut(s) 115, 339, 777, 793
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PpuMI RGGWCCY 1 cut(s) 829
PsiI TTATAA 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 720
Psp5II RGGWCCY 1 cut(s) 829
PspN4I GGNNCC 2 cut(s) 162, 680
PspPI GGNCC 2 cut(s) 679, 829
PspPPI RGGWCCY 1 cut(s) 829
PsuI RGATCY 2 cut(s) 160, 631
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
RseI CAYNNNNRTG 1 cut(s) 603
SacI GAGCTC 1 cut(s) 720
SapI GCTCTTC 1 cut(s) 36
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 4 cut(s) 114, 338, 776, 792
Sau3AI GATC 3 cut(s) 18, 160, 631
Sau96I GGNCC 2 cut(s) 679, 829
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 437, 438
SduI GDGCHC 1 cut(s) 720
SfaNI GCATC 3 cut(s) 790, 826, 829
SinI GGWCC 1 cut(s) 829
SmaI CCCGGG 1 cut(s) 438
SmiMI CAYNNNNRTG 1 cut(s) 603
Sse9I AATT 7 cut(s) 13, 79, 194, 262, 475, 570, 657
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 720
StyD4I CCNGG 2 cut(s) 435, 436
StyI CCWWGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 124, 454
TaqI TCGA 3 cut(s) 21, 57, 66
TasI AATT 7 cut(s) 13, 79, 194, 262, 475, 570, 657
TfiI GAWTC 3 cut(s) 371, 542, 735
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 853
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 4 cut(s) 113, 337, 775, 791
Tsp45I GTSAC 1 cut(s) 578
TspDTI ATGAA 3 cut(s) 384, 491, 801
TspGWI ACGGA 3 cut(s) 130, 176, 812
TspMI CCCGGG 1 cut(s) 436
TspRI CASTG 1 cut(s) 853
VpaK11BI GGWCC 1 cut(s) 829
XapI RAATTY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 659
XmaI CCCGGG 1 cut(s) 436
XmnI GAANNNNTTC 2 cut(s) 45, 593
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.