Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0003549

Physical Location & Seq

Genomic Coordinates Forward (+)

36384014 .. 36389067

Gene Structure 5054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1230400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGATCGCTCCCAATCCGAACAAGATGGCCACCAAAAGCAAAAGCAAGACCACCACTTTATGGAAATCCCAGAGTTGGACGACGATCCCTTCACGGAAGAAGACCTCCAAGCCATTGAAGCCGCATTCGAAGCTGCGACTTCCTCTCTCCCCAAAAAGCGAAGCTCCAGTCCAGAAGACGACGGCCCCGACCACAAAACCCAGCAACGCCGCCAGTCCCAGAGTGCCCGACGCCGACTGCGCAATTCAGTTCTCGCTCTCCAGCATCCAAACGCATTCCTTCTCTCGCCTTGCCGTCAAGCCAATGCAAGGATGAAATATCCCGTGATGATGTTTGGAGGTGAGATTAAATATAGCAGAACTGCAGTTGAAGTAGAGAAAGCTGCCTTGGAGATTTTAAAGAATTTTCAAGCAAAGAAAAAAGCAGTGGGTCAAAATGCAATTGGATTTGACATCGAGTGGAAACCCACATTTAAAAGAGGTGTTCCACCTGGGAAGGCTGCAGTTATGCAAATATGTGGAGGCACAAGTTGTTGTTATGTAATGCACATTGTACATTCTGGAATTCCTCGAAGTCTGCAGTTACTTCTCGAAGATTCTTCAATTTTGAAGGTTGGAGTTGGCATTGCCAATGATTGTCTTAAGGTTTTCAAAGATTATAATGTATCTACTAAAGCCGTAGAAGATCTTTCATATCTAGCTAAGCGGAAGGTTGTTGAAGGTCTGCAGAACTGGGGTCTTGCATCTCTAACTGAGAAGCTCATTTGCAAAGAGCTTTTGAAGCCCAACAAAATTAGATTGGGAAACTGGGAGGCCAAGTTTTTATCAAAGGATCAGTTACAGTATGCCGCAACTGATGCTTTCGCATCATGGTACCTTTACGAGGTGCTACTGGGCCTCCCTGATGCCGAAAAAGATACAGCCCGCAACCAGAGTGAGGAACTGCGAGCTGTGTCATTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004518 GO:0004527 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008408 GO:0009987 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol_A_exo1

Protein Analysis

321

Amino Acids

35.95

Weight (kDa)

8.39

Isoelectric Point (pI)

61.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_A_exo1	PF01612	133 - 297	3.3e-19	3'-5' exonuclease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003549)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34110 AT4G13870 AT4G13870 AT4G13885
fragaria_vesca	FvH4_4g13310 FvH4_4g13310
malus_domestica	MD05G1230400.v1.1
prunus_persica	Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1 Prupe.1G164900_v2.0.a1
pyrus_communis	pycom04g01340
rosa_chinensis	RchiOBHm_Chr4g0413601
rosa_laevigata	RLG00000008207
rosa_roxburghii	Rroxscaffold_1G00029650 Rroxscaffold_3G00236750 Rroxscaffold_3G00240660 Rroxscaffold_5G00357530
rosa_rugosa	Rorug04G0121400
rosa_samantha	Rh4AG181100 Rh4BG180300 Rh4CG192200 Rh4DG176400
rosa_wichuraiana	Rw4G015130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	660
Acc16I	TGCGCA	1 cut(s)	242
Acc65I	GGTACC	1 cut(s)	873
AccB1I	GGYRCC	1 cut(s)	873
AccB7I	CCANNNNNTGG	1 cut(s)	61
AciI	CCGC	5 cut(s)	123, 211, 706, 849, 925
AclWI	GGATC	3 cut(s)	12, 80, 840
AcoI	YGGCCR	1 cut(s)	28
AcsI	RAATTY	2 cut(s)	403, 564
AcyI	GRCGYC	1 cut(s)	232
AfaI	GTAC	2 cut(s)	555, 875
AfiI	CCNNNNNNNGG	5 cut(s)	61, 76, 309, 883, 937
AflII	CTTAAG	1 cut(s)	641
AgsI	TTSAA	8 cut(s)	119, 371, 410, 603, 610, 652, 719, 781
AjnI	CCWGG	1 cut(s)	490
AjuI	GAANNNNNNNTTGG	2 cut(s)	371, 403
AluBI	AGCT	7 cut(s)	134, 165, 383, 701, 760, 775, 950
AluI	AGCT	7 cut(s)	134, 165, 383, 701, 760, 775, 950
AlwI	GGATC	3 cut(s)	12, 80, 840
AoxI	GGCC	4 cut(s)	28, 184, 813, 895
ApeKI	GCWGC	3 cut(s)	134, 383, 500
ApoI	RAATTY	2 cut(s)	403, 564
ArsI	GACNNNNNNTTYG	2 cut(s)	154, 186
Asp718I	GGTACC	1 cut(s)	873
AspLEI	GCGC	1 cut(s)	243
AspS9I	GGNCC	2 cut(s)	185, 895
AsuHPI	GGTGA	1 cut(s)	353
AsuII	TTCGAA	1 cut(s)	129
BaeGI	GKGCMC	1 cut(s)	229
BalI	TGGCCA	1 cut(s)	30
BanI	GGYRCC	1 cut(s)	873
BbsI	GAAGAC	2 cut(s)	108, 183
BbvI	GCAGC	3 cut(s)	121, 370, 487
BccI	CCATC	1 cut(s)	20
BceAI	ACGGC	3 cut(s)	199, 279, 662
BciT130I	CCWGG	1 cut(s)	492
BfaI	CTAG	1 cut(s)	698
BfmI	CTRYAG	4 cut(s)	363, 501, 578, 725
BfrI	CTTAAG	1 cut(s)	641
BglII	AGATCT	1 cut(s)	685
BisI	GCNGC	6 cut(s)	123, 135, 211, 384, 501, 849
BlpI	GCTNAGC	1 cut(s)	702
BlsI	GCNGC	6 cut(s)	124, 136, 212, 385, 502, 850
Bme1390I	CCNGG	1 cut(s)	492
BmgT120I	GGNCC	2 cut(s)	185, 895
BmiI	GGNNCC	2 cut(s)	187, 875
BmrFI	CCNGG	1 cut(s)	492
BmrI	ACTGGG	3 cut(s)	742, 817, 902
BmsI	GCATC	5 cut(s)	274, 752, 847, 875, 895
BmuI	ACTGGG	3 cut(s)	742, 817, 902
BpiI	GAAGAC	2 cut(s)	108, 183
BpmI	CTGGAG	2 cut(s)	151, 245
Bpu1102I	GCTNAGC	1 cut(s)	702
Bpu14I	TTCGAA	1 cut(s)	129
BsaHI	GRCGYC	1 cut(s)	232
BsaJI	CCNNGG	2 cut(s)	387, 491
BsaXI	ACNNNNNCTCC	4 cut(s)	803, 833, 882, 912
Bsc4I	CCNNNNNNNGG	5 cut(s)	61, 76, 309, 883, 937
Bse1I	ACTGG	5 cut(s)	168, 214, 737, 812, 897
Bse3DI	GCAATG	1 cut(s)	624
BseBI	CCWGG	1 cut(s)	492
BseDI	CCNNGG	2 cut(s)	387, 491
BseGI	GGATG	2 cut(s)	265, 318
BseLI	CCNNNNNNNGG	5 cut(s)	61, 76, 309, 883, 937
BseMI	GCAATG	1 cut(s)	624
BseMII	CTCAG	1 cut(s)	744
BseNI	ACTGG	5 cut(s)	168, 214, 737, 812, 897
BseSI	GKGCMC	1 cut(s)	229
BseXI	GCAGC	3 cut(s)	121, 370, 487
BseYI	CCCAGC	1 cut(s)	201
BshFI	GGCC	4 cut(s)	30, 186, 815, 897
BshNI	GGYRCC	1 cut(s)	873
BslFI	GGGAC	1 cut(s)	202
BslI	CCNNNNNNNGG	5 cut(s)	61, 76, 309, 883, 937
BsmFI	GGGAC	1 cut(s)	202
BsmI	GAATGC	2 cut(s)	125, 275
BsnI	GGCC	4 cut(s)	30, 186, 815, 897
Bsp119I	TTCGAA	1 cut(s)	129
Bsp1286I	GDGCHC	1 cut(s)	229
Bsp1407I	TGTACA	1 cut(s)	553
Bsp143I	GATC	4 cut(s)	4, 85, 685, 832
Bsp1720I	GCTNAGC	1 cut(s)	702
BspACI	CCGC	5 cut(s)	123, 211, 706, 849, 925
BspANI	GGCC	4 cut(s)	30, 186, 815, 897
BspCNI	CTCAG	1 cut(s)	745
BspLI	GGNNCC	2 cut(s)	187, 875
BspMAI	CTGCAG	4 cut(s)	367, 505, 582, 729
BspPI	GGATC	3 cut(s)	12, 80, 840
BspT104I	TTCGAA	1 cut(s)	129
BspT107I	GGYRCC	1 cut(s)	873
BspTI	CTTAAG	1 cut(s)	641
BsrDI	GCAATG	1 cut(s)	624
BsrGI	TGTACA	1 cut(s)	553
BsrI	ACTGG	5 cut(s)	168, 214, 737, 812, 897
BssECI	CCNNGG	2 cut(s)	387, 491
BssMI	GATC	4 cut(s)	4, 85, 685, 832
BssNI	GRCGYC	1 cut(s)	232
BssT1I	CCWWGG	1 cut(s)	387
Bst2UI	CCWGG	1 cut(s)	492
Bst4CI	ACNGT	1 cut(s)	843
BstACI	GRCGYC	1 cut(s)	232
BstAFI	CTTAAG	1 cut(s)	641
BstAPI	GCANNNNNTGC	1 cut(s)	857
BstAUI	TGTACA	1 cut(s)	553
BstBI	TTCGAA	1 cut(s)	129
BstC8I	GCNNGC	2 cut(s)	925, 948
BstDEI	CTNAG	2 cut(s)	702, 753
BstENI	CCTNNNNNAGG	1 cut(s)	881
BstF5I	GGATG	2 cut(s)	265, 318
BstHHI	GCGC	1 cut(s)	243
BstKTI	GATC	4 cut(s)	7, 88, 688, 835
BstMBI	GATC	4 cut(s)	4, 85, 685, 832
BstMWI	GCNNNNNNNGC	5 cut(s)	119, 131, 240, 781, 857
BstNI	CCWGG	1 cut(s)	492
BstSCI	CCNGG	1 cut(s)	490
BstSFI	CTRYAG	4 cut(s)	363, 501, 578, 725
BstSLI	GKGCMC	1 cut(s)	229
BstV1I	GCAGC	3 cut(s)	121, 370, 487
BstV2I	GAAGAC	2 cut(s)	108, 183
BstX2I	RGATCY	1 cut(s)	685
BstYI	RGATCY	1 cut(s)	685
BsuRI	GGCC	4 cut(s)	30, 186, 815, 897
BtsCI	GGATG	2 cut(s)	265, 318
BtsI	GCAGTG	1 cut(s)	432
BtsIMutI	CAGTG	1 cut(s)	432
Cac8I	GCNNGC	2 cut(s)	925, 948
CfoI	GCGC	1 cut(s)	243
Cfr13I	GGNCC	2 cut(s)	185, 895
CseI	GACGC	1 cut(s)	240
Csp6I	GTAC	2 cut(s)	554, 874
CviAII	CATG	1 cut(s)	870
CviQI	GTAC	2 cut(s)	554, 874
DdeI	CTNAG	2 cut(s)	702, 753
DpnI	GATC	4 cut(s)	6, 87, 687, 834
DpnII	GATC	4 cut(s)	4, 85, 685, 832
DraI	TTTAAA	2 cut(s)	399, 475
EaeI	YGGCCR	1 cut(s)	28
Eco130I	CCWWGG	1 cut(s)	387
EcoNI	CCTNNNNNAGG	1 cut(s)	881
EcoRI	GAATTC	1 cut(s)	564
EcoRII	CCWGG	1 cut(s)	490
EcoT14I	CCWWGG	1 cut(s)	387
ErhI	CCWWGG	1 cut(s)	387
FaeI	CATG	1 cut(s)	873
FaiI	YATR	9 cut(s)	62, 354, 509, 517, 540, 660, 694, 846, 871
FaqI	GGGAC	1 cut(s)	202
FatI	CATG	1 cut(s)	869
FauI	CCCGC	1 cut(s)	932
Fnu4HI	GCNGC	6 cut(s)	123, 135, 211, 384, 501, 849
FokI	GGATG	2 cut(s)	252, 325
Fsp4HI	GCNGC	6 cut(s)	123, 135, 211, 384, 501, 849
FspBI	CTAG	1 cut(s)	698
FspI	TGCGCA	1 cut(s)	242
GlaI	GCGC	1 cut(s)	242
GluI	GCNGC	6 cut(s)	123, 135, 211, 384, 501, 849
GsaI	CCCAGC	1 cut(s)	205
GsuI	CTGGAG	2 cut(s)	151, 245
HaeIII	GGCC	4 cut(s)	30, 186, 815, 897
HgaI	GACGC	1 cut(s)	240
HhaI	GCGC	1 cut(s)	243
Hin1I	GRCGYC	1 cut(s)	232
Hin1II	CATG	1 cut(s)	873
Hin6I	GCGC	1 cut(s)	241
HinP1I	GCGC	1 cut(s)	241
HinfI	GANTC	1 cut(s)	596
HphI	GGTGA	1 cut(s)	353
Hpy188I	TCNGA	1 cut(s)	19
Hpy188III	TCNNGA	3 cut(s)	173, 561, 590
Hpy99I	CGWCG	3 cut(s)	86, 185, 234
HpyAV	CCTTC	6 cut(s)	100, 290, 490, 604, 703, 713
HpyCH4III	ACNGT	1 cut(s)	843
HpyF10VI	GCNNNNNNNGC	5 cut(s)	119, 131, 240, 781, 857
HpyF3I	CTNAG	2 cut(s)	702, 753
Hsp92I	GRCGYC	1 cut(s)	232
Hsp92II	CATG	1 cut(s)	873
HspAI	GCGC	1 cut(s)	241
KpnI	GGTACC	1 cut(s)	877
Kzo9I	GATC	4 cut(s)	4, 85, 685, 832
LmnI	GCTCC	2 cut(s)	14, 170
Lsp1109I	GCAGC	3 cut(s)	121, 370, 487
LweI	GCATC	5 cut(s)	274, 752, 847, 875, 895
MaeI	CTAG	1 cut(s)	698
MaeIII	GTNAC	2 cut(s)	582, 837
MalI	GATC	4 cut(s)	6, 87, 687, 834
MboI	GATC	4 cut(s)	4, 85, 685, 832
MboII	GAAGA	6 cut(s)	110, 113, 188, 591, 605, 695
MfeI	CAATTG	1 cut(s)	441
MflI	RGATCY	1 cut(s)	685
MhlI	GDGCHC	1 cut(s)	229
MlsI	TGGCCA	1 cut(s)	30
MluCI	AATT	6 cut(s)	244, 403, 441, 564, 603, 792
MluNI	TGGCCA	1 cut(s)	30
MmeI	TCCRAC	2 cut(s)	57, 595
Mox20I	TGGCCA	1 cut(s)	30
MscI	TGGCCA	1 cut(s)	30
MseI	TTAA	4 cut(s)	348, 398, 474, 642
Msp20I	TGGCCA	1 cut(s)	30
MspCI	CTTAAG	1 cut(s)	641
MspR9I	CCNGG	1 cut(s)	492
MunI	CAATTG	1 cut(s)	441
Mva1269I	GAATGC	2 cut(s)	125, 275
MvaI	CCWGG	1 cut(s)	492
MwoI	GCNNNNNNNGC	5 cut(s)	119, 131, 240, 781, 857
NdeII	GATC	4 cut(s)	4, 85, 685, 832
NlaIII	CATG	1 cut(s)	873
NlaIV	GGNNCC	2 cut(s)	187, 875
NsbI	TGCGCA	1 cut(s)	242
NspV	TTCGAA	1 cut(s)	129
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	2 cut(s)	125, 275
PfeI	GAWTC	1 cut(s)	596
PflMI	CCANNNNNTGG	1 cut(s)	61
PkrI	GCNGC	6 cut(s)	124, 136, 212, 385, 502, 850
PsiI	TTATAA	1 cut(s)	660
Psp6I	CCWGG	1 cut(s)	490
PspFI	CCCAGC	1 cut(s)	201
PspGI	CCWGG	1 cut(s)	490
PspN4I	GGNNCC	2 cut(s)	187, 875
PspPI	GGNCC	2 cut(s)	185, 895
PstI	CTGCAG	4 cut(s)	367, 505, 582, 729
PsuI	RGATCY	1 cut(s)	685
RsaI	GTAC	2 cut(s)	555, 875
RsaNI	GTAC	2 cut(s)	554, 874
SaqAI	TTAA	4 cut(s)	348, 398, 474, 642
SatI	GCNGC	6 cut(s)	123, 135, 211, 384, 501, 849
Sau3AI	GATC	4 cut(s)	4, 85, 685, 832
Sau96I	GGNCC	2 cut(s)	185, 895
ScrFI	CCNGG	1 cut(s)	492
SduI	GDGCHC	1 cut(s)	229
SfaNI	GCATC	5 cut(s)	274, 752, 847, 875, 895
SfcI	CTRYAG	4 cut(s)	363, 501, 578, 725
SfuI	TTCGAA	1 cut(s)	129
SmlI	CTYRAG	1 cut(s)	641
SmoI	CTYRAG	1 cut(s)	641
Sse9I	AATT	6 cut(s)	244, 403, 441, 564, 603, 792
SsiI	CCGC	5 cut(s)	123, 211, 706, 849, 925
SspMI	CTAG	1 cut(s)	698
StyD4I	CCNGG	1 cut(s)	490
StyI	CCWWGG	1 cut(s)	387
TaaI	ACNGT	1 cut(s)	843
TaqI	TCGA	4 cut(s)	129, 456, 571, 591
TasI	AATT	6 cut(s)	244, 403, 441, 564, 603, 792
TatI	WGTACW	1 cut(s)	553
TauI	GCSGC	3 cut(s)	125, 213, 851
TfiI	GAWTC	1 cut(s)	596
Tru1I	TTAA	4 cut(s)	348, 398, 474, 642
Tru9I	TTAA	4 cut(s)	348, 398, 474, 642
TscAI	CASTG	1 cut(s)	432
TseI	GCWGC	3 cut(s)	134, 383, 500
TspDTI	ATGAA	2 cut(s)	329, 681
TspGWI	ACGGA	1 cut(s)	110
TspRI	CASTG	1 cut(s)	432
Van91I	CCANNNNNTGG	1 cut(s)	61
Vha464I	CTTAAG	1 cut(s)	641
XagI	CCTNNNNNAGG	1 cut(s)	881
XapI	RAATTY	2 cut(s)	403, 564
XspI	CTAG	1 cut(s)	698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.