RLG00000008207

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0003549
Physical Location & Seq
Reverse (-)
27078172 .. 27083038
4867 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008207

Sequence Viewer

Length: 984 bp
ATGGAGCACTCGGAATCTCAAAATGACAACAAAGGCAAAGCCATCAGCACCACCTTATCGGAATCCGAATGGGAGGAGCCCTTCACAGAGGAAGACCTCGAAGCCATTGAAGCAGCATTCGAAGCCGCCGCTGCGACTTTTTCTCTCTCCAAGAAGCGACGCTCCAATCCAGACGACGAAGTCGCGGACCAGTCCCAGAGGGCCCGGCGCCGACTGCCGAGTTCGGTCCTCGCTCTCCAGCATCCAAACGCCTTCGCTCTCTCACCTTGTCGTCAAGCTGCCAATATAAGGATGAGATATCCTGTGTTGAAGTTTGGAGGTCAGGTTACATATAGCAGGACTGCAGTTGAAGTGGAGAAAGCTGCCATGGAGATTTTAAAGACTGTGAAAGCAAAGAAAAATGAAATGGGTCAAAATGCAGTTGGATTTGATATTGAGTGGAAACCCACATTCCAAAGAGGTGTTCCACCTGGGAAGGTTGCAGTTCTGCAGATATGTGGAGACACAAGTTCTTGCCATGTGATGCATATCATTCATTCTGGAATTCCTCGAAGTCTGCAGTTACTTCTTGAAAGTTCTTCGATTTTGAAGGTTGGAGCTGGTATCGCTAATGATGCTGTTAAGGTTTTCAAGGATTATAATGTATCTATTAAAGCTGTGGAGGATCTTTCATATCTGGCTAATCATAAGCTTGGAGATTCACAAAATTGGGGTCTTGCATCCCTAACTGAGAAGCTCATTTGTAAACAGCTTCTAAAGCCCAAAAAAATTCGATTGGGTAACTGGGAGACCAAATATTTATCAAAAGAGCAGCTGCAGTATGCTGCCACTGATGCATTTGCATCATGGTATCTTCATGAGGTGCTAAGGAGCCTCCCTGATGCTGAAAAACTTGAAAAAGTTACAGCAGACAACCAGAGTGAGGGACAAGCTGTATCATGTAATTACTTTCAGACTGAGGAACCACAAGCTGCATCATTGTAA

Protein Analysis

328

Amino Acids

36.29

Weight (kDa)

6.42

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 119 - 290 1.6e-20 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AasI GACNNNNNNGTC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 207
AccII CGCG 1 cut(s) 185
AciI CCGC 3 cut(s) 126, 129, 185
AclWI GGATC 1 cut(s) 672
AcsI RAATTY 2 cut(s) 543, 768
AcyI GRCGYC 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 288, 922
AgsI TTSAA 7 cut(s) 110, 310, 350, 572, 589, 631, 896
AjnI CCWGG 1 cut(s) 469
AjuI GAANNNNNNNTTGG 2 cut(s) 447, 479
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 495, 782
AlwI GGATC 1 cut(s) 672
AoxI GGCC 1 cut(s) 201
ApaI GGGCCC 1 cut(s) 205
ApeKI GCWGC 8 cut(s) 113, 131, 278, 362, 811, 814, 824, 971
ApoI RAATTY 2 cut(s) 543, 768
ArsI GACNNNNNNTTYG 2 cut(s) 697, 729
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 4 cut(s) 187, 201, 202, 226
AsuC2I CCSGG 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 255
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 2 cut(s) 187, 226
BaeGI GKGCMC 1 cut(s) 205
BanI GGYRCC 1 cut(s) 207
BanII GRGCYC 2 cut(s) 81, 205
BbsI GAAGAC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 8 cut(s) 118, 125, 265, 349, 801, 811, 823, 958
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 471
BcnI CCSGG 1 cut(s) 205
BcoDI GTCTC 2 cut(s) 495, 782
BfmI CTRYAG 4 cut(s) 342, 488, 557, 815
BfoI RGCGCY 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 205, 471
Bme18I GGWCC 2 cut(s) 187, 226
BmgT120I GGNCC 4 cut(s) 187, 201, 202, 226
BmiI GGNNCC 5 cut(s) 78, 203, 209, 872, 963
BmrFI CCNGG 2 cut(s) 205, 471
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 7 cut(s) 250, 513, 604, 728, 823, 851, 871
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 221
Bpu10I CCTNAGC 1 cut(s) 866
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 782
BsaJI CCNNGG 2 cut(s) 366, 470
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 922
Bse1I ACTGG 2 cut(s) 190, 788
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 2 cut(s) 366, 470
BseGI GGATG 3 cut(s) 241, 297, 719
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 288, 922
BseMII CTCAG 2 cut(s) 720, 948
BseNI ACTGG 2 cut(s) 190, 788
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 205
BseXI GCAGC 8 cut(s) 118, 125, 265, 349, 801, 811, 823, 958
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 1 cut(s) 203
BshNI GGYRCC 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 2 cut(s) 178, 939
BslI CCNNNNNNNGG 2 cut(s) 288, 922
BsmAI GTCTC 2 cut(s) 495, 782
BsmFI GGGAC 2 cut(s) 178, 939
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 782
Bsp119I TTCGAA 1 cut(s) 120
Bsp120I GGGCCC 1 cut(s) 201
Bsp1286I GDGCHC 3 cut(s) 9, 81, 205
Bsp143I GATC 1 cut(s) 664
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 3 cut(s) 126, 129, 185
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 2 cut(s) 721, 949
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 5 cut(s) 78, 203, 209, 872, 963
BspMAI CTGCAG 4 cut(s) 346, 492, 561, 819
BspPI GGATC 1 cut(s) 672
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 207
BspTNI GGTCTC 1 cut(s) 782
BsrI ACTGG 2 cut(s) 190, 788
BssECI CCNNGG 2 cut(s) 366, 470
BssMI GATC 1 cut(s) 664
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 385
BstACI GRCGYC 1 cut(s) 208
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 3 cut(s) 729, 866, 957
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 241, 297, 719
BstFNI CGCG 1 cut(s) 185
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 1 cut(s) 667
BstMAI GTCTC 2 cut(s) 495, 782
BstMBI GATC 1 cut(s) 664
BstMWI GCNNNNNNNGC 8 cut(s) 110, 122, 131, 214, 605, 614, 757, 833
BstNI CCWGG 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 203, 469
BstSFI CTRYAG 4 cut(s) 342, 488, 557, 815
BstSLI GKGCMC 1 cut(s) 205
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 8 cut(s) 118, 125, 265, 349, 801, 811, 823, 958
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 3 cut(s) 241, 297, 719
BtsIMutI CAGTG 1 cut(s) 828
CciI TCATGA 1 cut(s) 856
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 4 cut(s) 187, 201, 202, 226
CseI GACGC 1 cut(s) 168
CviAII CATG 5 cut(s) 367, 518, 846, 857, 939
DdeI CTNAG 3 cut(s) 729, 866, 957
DinI GGCGCC 1 cut(s) 209
DpnI GATC 1 cut(s) 666
DpnII GATC 1 cut(s) 664
DraI TTTAAA 1 cut(s) 378
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 81, 205
Eco31I GGTCTC 1 cut(s) 782
Eco32I GATATC 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 187, 226
EcoO109I RGGNCCY 1 cut(s) 201
EcoRI GAATTC 1 cut(s) 543
EcoRII CCWGG 1 cut(s) 469
EcoRV GATATC 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 2 cut(s) 528, 838
EcoT38I GRGCYC 2 cut(s) 81, 205
EgeI GGCGCC 1 cut(s) 209
EheI GGCGCC 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 5 cut(s) 370, 521, 849, 860, 942
FaqI GGGAC 2 cut(s) 178, 939
FatI CATG 5 cut(s) 366, 517, 845, 856, 938
FokI GGATG 3 cut(s) 228, 304, 706
FriOI GRGCYC 2 cut(s) 81, 205
GlaI GCGC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 221
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 205
HgaI GACGC 1 cut(s) 168
HhaI GCGC 1 cut(s) 210
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 5 cut(s) 370, 521, 849, 860, 942
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 3 cut(s) 14, 62, 698
HpaII CCGG 1 cut(s) 205
HphI GGTGA 1 cut(s) 255
Hpy166II GTNNAC 1 cut(s) 746
Hpy188I TCNGA 4 cut(s) 13, 61, 67, 954
Hpy188III TCNNGA 4 cut(s) 170, 540, 569, 857
Hpy8I GTNNAC 1 cut(s) 746
Hpy99I CGWCG 2 cut(s) 162, 179
HpyAV CCTTC 4 cut(s) 91, 262, 469, 583
HpyCH4III ACNGT 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 8 cut(s) 110, 122, 131, 214, 605, 614, 757, 833
HpyF3I CTNAG 3 cut(s) 729, 866, 957
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 5 cut(s) 370, 521, 849, 860, 942
HspAI GCGC 1 cut(s) 208
KasI GGCGCC 1 cut(s) 207
Kzo9I GATC 1 cut(s) 664
LmnI GCTCC 5 cut(s) 4, 76, 167, 596, 870
Lsp1109I GCAGC 8 cut(s) 118, 125, 265, 349, 801, 811, 823, 958
LweI GCATC 7 cut(s) 250, 513, 604, 728, 823, 851, 871
MaeIII GTNAC 4 cut(s) 325, 561, 779, 901
MalI GATC 1 cut(s) 666
MboI GATC 1 cut(s) 664
MboII GAAGA 3 cut(s) 104, 570, 845
MflI RGATCY 1 cut(s) 664
MhlI GDGCHC 3 cut(s) 9, 81, 205
MluCI AATT 4 cut(s) 543, 706, 768, 943
Mly113I GGCGCC 1 cut(s) 208
MmeI TCCRAC 2 cut(s) 403, 574
Mph1103I ATGCAT 2 cut(s) 528, 838
MseI TTAA 3 cut(s) 377, 621, 651
MspA1I CMGCKG 2 cut(s) 131, 814
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 2 cut(s) 205, 471
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 8 cut(s) 110, 122, 131, 214, 605, 614, 757, 833
NarI GGCGCC 1 cut(s) 208
NciI CCSGG 1 cut(s) 205
NcoI CCATGG 1 cut(s) 366
NdeII GATC 1 cut(s) 664
NlaIII CATG 5 cut(s) 370, 521, 849, 860, 942
NlaIV GGNNCC 5 cut(s) 78, 203, 209, 872, 963
NmeAIII GCCGAG 1 cut(s) 243
NsiI ATGCAT 2 cut(s) 528, 838
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 1 cut(s) 856
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 3 cut(s) 14, 62, 698
PflFI GACNNNGTC 1 cut(s) 179
PluTI GGCGCC 1 cut(s) 211
PsiI TTATAA 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 469
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 5 cut(s) 78, 203, 209, 872, 963
PspOMI GGGCCC 1 cut(s) 201
PspPI GGNCC 4 cut(s) 187, 201, 202, 226
PstI CTGCAG 4 cut(s) 346, 492, 561, 819
PsuI RGATCY 1 cut(s) 664
PsyI GACNNNGTC 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 814
SaqAI TTAA 3 cut(s) 377, 621, 651
Sau3AI GATC 1 cut(s) 664
Sau96I GGNCC 4 cut(s) 187, 201, 202, 226
ScrFI CCNGG 2 cut(s) 205, 471
SduI GDGCHC 3 cut(s) 9, 81, 205
SfaNI GCATC 7 cut(s) 250, 513, 604, 728, 823, 851, 871
SfcI CTRYAG 4 cut(s) 342, 488, 557, 815
SfoI GGCGCC 1 cut(s) 209
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 2 cut(s) 187, 226
Sse9I AATT 4 cut(s) 543, 706, 768, 943
SsiI CCGC 3 cut(s) 126, 129, 185
SspDI GGCGCC 1 cut(s) 207
SspI AATATT 1 cut(s) 797
StyD4I CCNGG 2 cut(s) 203, 469
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 385
TaqI TCGA 5 cut(s) 99, 120, 550, 581, 772
TaqII GACCGA 1 cut(s) 214
TasI AATT 4 cut(s) 543, 706, 768, 943
TauI GCSGC 2 cut(s) 128, 131
TfiI GAWTC 3 cut(s) 14, 62, 698
Tru1I TTAA 3 cut(s) 377, 621, 651
Tru9I TTAA 3 cut(s) 377, 621, 651
TscAI CASTG 1 cut(s) 835
TseI GCWGC 8 cut(s) 113, 131, 278, 362, 811, 814, 824, 971
TspDTI ATGAA 4 cut(s) 417, 524, 660, 845
TspRI CASTG 1 cut(s) 835
Tth111I GACNNNGTC 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 187, 226
XapI RAATTY 2 cut(s) 543, 768
Zsp2I ATGCAT 2 cut(s) 528, 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.