Rorug04G0121400

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003549
Physical Location & Seq
Forward (+)
19219987 .. 19223770
3784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0121400.1

Sequence Viewer

Length: 3336 bp
ATGGGAAACCCACGAATGCTAATCCCATTTGGACTAGTCGTGTTACTTCACCACTCATTCTTCCTTCAACCAGCCACCACCACCTCCTCCTCCAACTTCACCGATCAATCTGCTCTCCTTGCCATTCAATCCAAACTCACATTTGATCCTACCGAAACTGTCTTGGGTGGTAATTGGAGTACCAACACAAGCTTCTGTGATTGGATTGGGGTCTCATGCAGTAAACGAAGGCAAAGAGTCACCGCCGTGGACTTGTCATCTATGGGTCTTGAAGGCACCATTGCACCTCATGTTGGCAACCTCTCCTTTCTGGTCATATTGGTTCTTCAATACAACAGCTTCTCTGGGTTTGTGCCAAATGAAATCAGCCATTTACACCGCTTGAAGGTACTTGTACTGGAAGGGAACCAGTTGGAAGGGCACATCCCTGCAACCTTATATCATTGCCAAAAGCTCGAAATCATTGCCCTTGCAGAAAACAAGCTTACCGGTCGTATACCTGAAGAGTTGGGTTTCTTACCCAAACTTCAGAAATTATATCTCGGTTTAAACAATCTCACGGCTGGTGAAATTCCAGCATCTTTGGGGAACGTTTCAACATTACAAGAATTGAGTGTGGCAAAAAGTGGCCTCACAGGTTCACTCCCTTCCACTCTACTTAACCTATCTTCTCTAGTTGTTATTGTACTATATACAAATAACATTTCAGGAGTTCTTCCTGAGGATCTTTGCCATTACTGGCCTAATATTCAACACATTGCTTTATCTCGAAACAAGCTTGGCGGCCAGCTCTCTTCGAGATTTAATCAATGCAGTAACATTGAGGTGTTATCTCTGTCATACAATAGGTTTGTCGGAACTATTCCAAAAGACCTTGCAAGTTTACAGCATCTTGAATTGCTCTACCTCGGTGGTAATGGCTTAACCGGTACTGTACCGCCGGTCCTAGGTAACTTGTCGAACTTGCAAAAGTTTGCTGTAGAACGTAATAACATCAAAGGAAGTATTCCCAGAGAGTTGGGCCTTCTTTCTAATTTACTTTACCTTTCATTAGCTATGAATAGCCTAACAGGTGCAATACCTCCAGAAATTTTCAACATCACCTCCTTACAAACTTTGTCAGTACGTAATAATTCCCTTTCTGGGGCAATTCCTTCATATTCTGGAGTAGTTTTTCCTAACCTCGAATTCCTATACTTAGGAAGCAATAAGATTACTGGTCTTATCCCATCCTTTTTTTCAAACCTTACTAAGCTGAATGACTTGGGACTCAGTACCAATTTACTCTATGGACCAATTCCCATGGATCTTGGTAGCCTAAAACACCTCCAGTTCCTTGATTTGGCAAGAAATCAGTTGACTGGAGAACCTGGAGTTCCTGAACTTAAATTCCTTTCCTCATTATTCAATTCCACTTCGCTGCGAGAGTTGGTTCTGACTGAAAATCCTCTAAATGGCATCATACCCAATTCCTTTGGAAATTTTTCTTCCCTTCAGCACATTTCAGCAATTTCGTGCCAGATAAAGGGTCACATTCCCACAAAAATTCATTCCTTGAAAAGTTTAATCTACCTTAAATTTGTGGACAACAATATAAGTGGAAAAATACCACCACAGATTGGAGGACTGGAGAGGTTACAAAGGTTGTATCTGAGCTTCAACAGAATTGAAGGATCCATTCCATATGAGATCTGTCATCTGCAGAACCTAGGAGAACTATCACTAGAGAATAACAAAATCTCTGGATTGATCCCAAATTGCATTGGAAACCTCAGCCGCACCCTTCAAATATTTCTCCTAAATTCCAACCAAATGACATCCTTGATACCAATGAATTTATGGAACTCTGAAAATCTCTTGGTGTTAAATTTGTCATCCAATTCTTTCAGTGGAGACATACCTACCACCTTGACAAAGTCGATTTCCATTGAAGTGATTGATTTATCTCATAACCAAATTACTGGTAATATTTCAAGCATCATAGCAGCATTCCAAAGCCTAGCTTACCTTGATTTGTCAAAGAACTGGTTTATAGGATCCATCCCACCGAGCTGTGGTGATCTGAAAGGGTTAGAGTATCTGGACCTATCATACAATAACCTGTCTGGTGCAATACCAAAGTCTCTTGAAACTCTCAAATTTCTCGAGTATTTGAATCTCTCTTTCAATGATCTCTTTGGAGAGATTCCTTCTGGTGGACCTTTTGTAAACTTTAGTTCCCGATCATTTTTGGAGAATAAAGCACTTTGTGGGAGACCAAAGTATGGAGTTCCACCTTGTATGACTCAAAAATCAAAGACGACACATCCTGTGCTCACATATGTTCTTCCTAGTATTGCATCGACAGTGATTCTGATAGCCCTCATTTATATGATCACATGGTATAGAAAAAGAAAGAGAGGCAAGAGTACAACAGTTGAACAGCTAACAACAGTGGAGCATAGTAGGATATCATATCAAGAACTCCGTCGCGCTACAAATGACTTTTGTGAAAGCAACTTTCTTGGAGGGGGAAGTTTTGGCTCGGTGTACAAGGGGATACTGTCTAATGGGACTACTATTGCTGTCAAAATTCTTAAGCTGGAAATGGAGGGTGCTGTAAAAAGTTTTGATGCAGAATGCAAGGTGTGGCGAACAATCCGGCATAGGAATCTTGTAAAGGTCATAACTACATGTTCTAGCCCTGAGGTCAGAGCTTTGGTGCTGCAGTACATGTCTAATGGCAGTCTTGAGAAGTGGTTATACTCTCACAACTACTGTCTCAGTATTCTGCAAAGAGTGAGCATGTTGATCGATATTGCATCTGCTTTGGAATATCTCCATCATGGTCAAGCAGAAGCAGTGGTGCACTGTGATGTGAAGCCTGGGAATATCCTTCTTGATGAAGACATGTTTGCACATGTTGCAGACTTCGGTCTTGCAAAAATTTTAGCAAAAAACAAAGAGGAAACACAGACAAGAACCATAGGCACTCTGGGCTACGTAGCACCAGAGTATGGTTCTGCAGGAAAAGTGTCCAAAAAGGGTGATGTCTATAGCTTTGGGATAATGCTGCTGGAAATAATGGCGAGAAAGAAACCCACTGATGAAATGTTTGCCGGAGAAGTGACTTTGAGGCAATGGATAAATGTATCTGTCCCCGATTATGTTCTGGAAGTGGTGGATGCTGGCTTATTGAGCATAGAAGAGGGAAGAGACATAAATGCCACAGAAAGTATCCTAGTGTCCATCCTGGAAATAGGCTTGAGGTGTTCGGAAGAAGCAGCAGAGGATAGAATGGATATTAAGGACGTGGTGCCCAAGCTCAAGAAAATAAAATTGGCACTACAACATTGA

Protein Analysis

1111

Amino Acids

122.14

Weight (kDa)

7.56

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 248 - 308 2.8e-06 Leucine rich repeat
LRR_14 PF23598 338 - 477 6.4e-08 Leucine-rich repeat region
LRR_8 PF13855 393 - 453 3e-08 Leucine rich repeat
LRR_14 PF23598 512 - 640 4.4e-08 Leucine-rich repeat region
LRR_8 PF13855 666 - 725 4.5e-10 Leucine rich repeat
Pkinase PF00069 833 - 1029 1.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 834 - 1101 6e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 275, 3295
AccB7I CCANNNNNTGG 3 cut(s) 1619, 2264, 2996
AccI GTMKAC 1 cut(s) 496
AccII CGCG 1 cut(s) 2472
AciI CCGC 5 cut(s) 243, 379, 783, 938, 1777
AclI AACGTT 1 cut(s) 591
AclWI GGATC 8 cut(s) 140, 732, 1314, 1668, 1681, 1744, 2031, 2044
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 3 cut(s) 512, 522, 1478
AdeI CACNNNGTG 1 cut(s) 2249
AflII CTTAAG 1 cut(s) 2576
AflIII ACRYGT 4 cut(s) 2672, 2712, 2889, 2899
AgeI ACCGGT 2 cut(s) 488, 926
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 3292
AjnI CCWGG 3 cut(s) 1369, 2863, 3231
AjuI GAANNNNNNNTTGG 4 cut(s) 176, 208, 3302, 3334
AleI CACNNNNGTG 1 cut(s) 245
AloI GAACNNNNNNTCC 2 cut(s) 1374, 1406
Alw21I GWGCWC 2 cut(s) 2316, 2850
Alw26I GTCTC 6 cut(s) 217, 1887, 2127, 2248, 2765, 3189
Alw44I GTGCAC 1 cut(s) 2846
AlwI GGATC 8 cut(s) 140, 732, 1314, 1668, 1681, 1744, 2031, 2044
Ama87I CYCGRG 1 cut(s) 2144
AoxI GGCC 4 cut(s) 628, 740, 784, 1021
ApaLI GTGCAC 1 cut(s) 2846
ApeKI GCWGC 5 cut(s) 1420, 1985, 2704, 3052, 3263
AsiGI ACCGGT 2 cut(s) 488, 926
Asp700I GAANNNNTTC 1 cut(s) 1483
AspA2I CCTAGG 2 cut(s) 946, 1708
AspLEI GCGC 1 cut(s) 2474
AspS9I GGNCC 5 cut(s) 943, 1021, 1292, 2083, 2198
AsuHPI GGTGA 7 cut(s) 41, 91, 232, 578, 1093, 2069, 3038
AvaI CYCGRG 1 cut(s) 2144
AvaII GGWCC 4 cut(s) 943, 1292, 2083, 2198
AvrII CCTAGG 2 cut(s) 946, 1708
AxyI CCTNAGG 2 cut(s) 720, 2685
BaeGI GKGCMC 3 cut(s) 423, 2850, 3300
BamHI GGATCC 2 cut(s) 1673, 2036
BanI GGYRCC 2 cut(s) 275, 3295
BarI GAAGNNNNNNTAC 4 cut(s) 1640, 1672, 2726, 2758
BbsI GAAGAC 1 cut(s) 2892
Bbv12I GWGCWC 2 cut(s) 2316, 2850
BbvCI CCTCAGC 1 cut(s) 1772
BbvI GCAGC 5 cut(s) 1407, 1997, 2691, 3039, 3275
BccI CCATC 4 cut(s) 1237, 2048, 2829, 3236
BceAI ACGGC 2 cut(s) 230, 576
BcgI CGANNNNNNTGC 6 cut(s) 92, 126, 446, 480, 2773, 2807
BciT130I CCWGG 3 cut(s) 1371, 2865, 3233
BciVI GTATCC 2 cut(s) 2532, 3227
BclI TGATCA 1 cut(s) 2373
BcoDI GTCTC 6 cut(s) 217, 1887, 2127, 2248, 2765, 3189
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 9 cut(s) 35, 674, 947, 1709, 1724, 2000, 2331, 2679, 3221
BfmI CTRYAG 5 cut(s) 978, 1700, 2705, 3003, 3034
BfrI CTTAAG 1 cut(s) 2576
BfuI GTATCC 2 cut(s) 2532, 3227
BglII AGATCT 1 cut(s) 1689
BisI GCNGC 7 cut(s) 784, 1421, 1777, 1986, 2705, 3053, 3264
BlnI CCTAGG 2 cut(s) 946, 1708
BlsI GCNGC 7 cut(s) 785, 1422, 1778, 1987, 2706, 3054, 3265
Bme1390I CCNGG 3 cut(s) 1371, 2865, 3233
Bme18I GGWCC 4 cut(s) 943, 1292, 2083, 2198
BmeT110I CYCGRG 1 cut(s) 2144
BmgBI CACGTC 1 cut(s) 3292
BmgT120I GGNCC 5 cut(s) 943, 1021, 1292, 2083, 2198
BmiI GGNNCC 5 cut(s) 277, 407, 1675, 2038, 3297
BmrFI CCNGG 3 cut(s) 1371, 2865, 3233
BmsI GCATC 8 cut(s) 587, 898, 1467, 1986, 2348, 2602, 2810, 3154
BoxI GACNNNNGTC 1 cut(s) 2913
BpiI GAAGAC 1 cut(s) 2892
BpmI CTGGAG 6 cut(s) 1068, 1185, 1313, 1383, 1392, 1649
Bpu10I CCTNAGC 1 cut(s) 1772
BpuEI CTTGAG 3 cut(s) 2750, 3265, 3290
Bsa29I ATCGAT 1 cut(s) 2793
BsaAI YACGTR 2 cut(s) 1127, 2983
BsaI GGTCTC 2 cut(s) 217, 2248
BsaJI CCNNGG 6 cut(s) 246, 907, 946, 1302, 1708, 2864
BsaWI WCCGGW 2 cut(s) 488, 926
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bse118I RCCGGY 3 cut(s) 488, 926, 940
Bse1I ACTGG 9 cut(s) 402, 409, 743, 1222, 1330, 1366, 1632, 1966, 2030
Bse21I CCTNAGG 2 cut(s) 720, 2685
Bse3DI GCAATG 5 cut(s) 279, 442, 462, 756, 3125
BseBI CCWGG 3 cut(s) 1371, 2865, 3233
BseCI ATCGAT 1 cut(s) 2793
BseDI CCNNGG 6 cut(s) 246, 907, 946, 1302, 1708, 2864
BseGI GGATG 8 cut(s) 423, 1229, 1817, 1874, 2040, 2305, 3169, 3228
BseMI GCAATG 5 cut(s) 279, 442, 462, 756, 3125
BseMII CTCAG 6 cut(s) 711, 1285, 1643, 1786, 2676, 2776
BseNI ACTGG 9 cut(s) 402, 409, 743, 1222, 1330, 1366, 1632, 1966, 2030
BseRI GAGGAG 2 cut(s) 76, 79
BseSI GKGCMC 3 cut(s) 423, 2850, 3300
BseXI GCAGC 5 cut(s) 1407, 1997, 2691, 3039, 3275
Bsh1236I CGCG 1 cut(s) 2472
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 4 cut(s) 630, 742, 786, 1023
BshNI GGYRCC 2 cut(s) 275, 3295
BshTI ACCGGT 2 cut(s) 488, 926
BshVI ATCGAT 1 cut(s) 2793
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 2 cut(s) 2316, 2850
BsiHKCI CYCGRG 1 cut(s) 2144
BsiSI CCGG 5 cut(s) 489, 927, 941, 2641, 3099
BslFI GGGAC 3 cut(s) 1281, 2566, 3122
BsmAI GTCTC 6 cut(s) 217, 1887, 2127, 2248, 2765, 3189
BsmFI GGGAC 3 cut(s) 1281, 2566, 3122
BsmI GAATGC 3 cut(s) 21, 1988, 2624
BsnI GGCC 4 cut(s) 630, 742, 786, 1023
Bso31I GGTCTC 2 cut(s) 217, 2248
BsoBI CYCGRG 1 cut(s) 2144
Bsp1286I GDGCHC 4 cut(s) 423, 2316, 2850, 3300
Bsp1407I TGTACA 1 cut(s) 2529
Bsp19I CCATGG 1 cut(s) 1302
BspACI CCGC 5 cut(s) 243, 379, 783, 938, 1777
BspANI GGCC 4 cut(s) 630, 742, 786, 1023
BspCNI CTCAG 6 cut(s) 712, 1284, 1644, 1785, 2677, 2775
BspDI ATCGAT 1 cut(s) 2793
BspFNI CGCG 1 cut(s) 2472
BspLI GGNNCC 5 cut(s) 277, 407, 1675, 2038, 3297
BspMAI CTGCAG 3 cut(s) 1704, 2709, 3007
BspPI GGATC 8 cut(s) 140, 732, 1314, 1668, 1681, 1744, 2031, 2044
BspT107I GGYRCC 2 cut(s) 275, 3295
BspTI CTTAAG 1 cut(s) 2576
BspTNI GGTCTC 2 cut(s) 217, 2248
BsrDI GCAATG 5 cut(s) 279, 442, 462, 756, 3125
BsrFI RCCGGY 3 cut(s) 488, 926, 940
BsrGI TGTACA 1 cut(s) 2529
BsrI ACTGG 9 cut(s) 402, 409, 743, 1222, 1330, 1366, 1632, 1966, 2030
BssAI RCCGGY 3 cut(s) 488, 926, 940
BssECI CCNNGG 6 cut(s) 246, 907, 946, 1302, 1708, 2864
BssNAI GTATAC 1 cut(s) 497
BssT1I CCWWGG 3 cut(s) 946, 1302, 1708
Bst1107I GTATAC 1 cut(s) 497
Bst2UI CCWGG 3 cut(s) 1371, 2865, 3233
Bst4CI ACNGT 8 cut(s) 160, 934, 2347, 2416, 2434, 2544, 2759, 2852
Bst6I CTCTTC 4 cut(s) 498, 799, 3180, 3187
BstAFI CTTAAG 1 cut(s) 2576
BstAPI GCANNNNNTGC 1 cut(s) 2903
BstAUI TGTACA 1 cut(s) 2529
BstBAI YACGTR 2 cut(s) 1127, 2983
BstC8I GCNNGC 2 cut(s) 788, 3169
BstDEI CTNAG 8 cut(s) 720, 1198, 1251, 1271, 1652, 1772, 2685, 2762
BstDSI CCRYGG 2 cut(s) 246, 1302
BstF5I GGATG 8 cut(s) 423, 1229, 1817, 1874, 2040, 2305, 3169, 3228
BstFNI CGCG 1 cut(s) 2472
BstHHI GCGC 1 cut(s) 2474
BstMAI GTCTC 6 cut(s) 217, 1887, 2127, 2248, 2765, 3189
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 4 cut(s) 119, 2903, 2976, 3177
BstNI CCWGG 3 cut(s) 1371, 2865, 3233
BstNSI RCATGY 5 cut(s) 2676, 2716, 2788, 2893, 2903
BstPAI GACNNNNGTC 1 cut(s) 2913
BstSCI CCNGG 3 cut(s) 1369, 2863, 3231
BstSFI CTRYAG 5 cut(s) 978, 1700, 2705, 3003, 3034
BstSLI GKGCMC 3 cut(s) 423, 2850, 3300
BstSNI TACGTA 2 cut(s) 1127, 2983
BstUI CGCG 1 cut(s) 2472
BstV1I GCAGC 5 cut(s) 1407, 1997, 2691, 3039, 3275
BstV2I GAAGAC 1 cut(s) 2892
BstX2I RGATCY 5 cut(s) 724, 1306, 1673, 1689, 2036
BstXI CCANNNNNNTGG 2 cut(s) 1018, 1961
BstYI RGATCY 5 cut(s) 724, 1306, 1673, 1689, 2036
BstZ17I GTATAC 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 2793
Bsu36I CCTNAGG 2 cut(s) 720, 2685
BsuI GTATCC 2 cut(s) 2532, 3227
BsuRI GGCC 4 cut(s) 630, 742, 786, 1023
BsuTUI ATCGAT 1 cut(s) 2793
BtgI CCRYGG 2 cut(s) 246, 1302
BtrI CACGTC 1 cut(s) 3292
BtsCI GGATG 8 cut(s) 423, 1229, 1817, 1874, 2040, 2305, 3169, 3228
BtsI GCAGTG 1 cut(s) 2847
BtsIMutI CAGTG 6 cut(s) 1894, 2352, 2439, 2847, 2848, 3081
Cac8I GCNNGC 2 cut(s) 788, 3169
CfoI GCGC 1 cut(s) 2474
Cfr10I RCCGGY 3 cut(s) 488, 926, 940
Cfr13I GGNCC 5 cut(s) 943, 1021, 1292, 2083, 2198
ClaI ATCGAT 1 cut(s) 2793
CspAI ACCGGT 2 cut(s) 488, 926
CspCI CAANNNNNGTGG 2 cut(s) 1579, 1614
DdeI CTNAG 8 cut(s) 720, 1198, 1251, 1271, 1652, 1772, 2685, 2762
DraI TTTAAA 1 cut(s) 549
DraIII CACNNNGTG 1 cut(s) 2249
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 4 cut(s) 498, 799, 3180, 3187
EarI CTCTTC 4 cut(s) 498, 799, 3180, 3187
Eco105I TACGTA 2 cut(s) 1127, 2983
Eco130I CCWWGG 3 cut(s) 946, 1302, 1708
Eco31I GGTCTC 2 cut(s) 217, 2248
Eco32I GATATC 1 cut(s) 2451
Eco47I GGWCC 4 cut(s) 943, 1292, 2083, 2198
Eco57I CTGAAG 3 cut(s) 512, 522, 1478
Eco81I CCTNAGG 2 cut(s) 720, 2685
Eco88I CYCGRG 1 cut(s) 2144
EcoRI GAATTC 1 cut(s) 1187
EcoRII CCWGG 3 cut(s) 1369, 2863, 3231
EcoRV GATATC 1 cut(s) 2451
EcoT14I CCWWGG 3 cut(s) 946, 1302, 1708
ErhI CCWWGG 3 cut(s) 946, 1302, 1708
FalI AAGNNNNNCTT 2 cut(s) 1987, 2019
FaqI GGGAC 3 cut(s) 1281, 2566, 3122
FauNDI CATATG 2 cut(s) 1684, 2320
FbaI TGATCA 1 cut(s) 2373
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 7 cut(s) 784, 1421, 1777, 1986, 2705, 3053, 3264
FokI GGATG 8 cut(s) 410, 1216, 1804, 1861, 2027, 2292, 3176, 3215
Fsp4HI GCNGC 7 cut(s) 784, 1421, 1777, 1986, 2705, 3053, 3264
FspBI CTAG 9 cut(s) 35, 674, 947, 1709, 1724, 2000, 2331, 2679, 3221
GlaI GCGC 1 cut(s) 2473
GluI GCNGC 7 cut(s) 784, 1421, 1777, 1986, 2705, 3053, 3264
GsuI CTGGAG 6 cut(s) 1068, 1185, 1313, 1383, 1392, 1649
HaeIII GGCC 4 cut(s) 630, 742, 786, 1023
HapII CCGG 5 cut(s) 489, 927, 941, 2641, 3099
HhaI GCGC 1 cut(s) 2474
Hin6I GCGC 1 cut(s) 2472
HinP1I GCGC 1 cut(s) 2472
HincII GTYRAC 1 cut(s) 1359
HindII GTYRAC 1 cut(s) 1359
HindIII AAGCTT 3 cut(s) 190, 482, 776
HinfI GANTC 7 cut(s) 237, 1269, 2155, 2185, 2284, 2350, 2650
HpaII CCGG 5 cut(s) 489, 927, 941, 2641, 3099
HphI GGTGA 7 cut(s) 41, 91, 232, 578, 1093, 2069, 3038
Hpy188I TCNGA 9 cut(s) 531, 857, 1437, 1653, 1849, 2064, 2355, 2693, 3256
Hpy99I CGWCG 1 cut(s) 2472
HpyCH4III ACNGT 8 cut(s) 160, 934, 2347, 2416, 2434, 2544, 2759, 2852
HpyCH4IV ACGT 5 cut(s) 591, 985, 1126, 2982, 3291
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 2903, 2976, 3177
HpyF3I CTNAG 8 cut(s) 720, 1198, 1251, 1271, 1652, 1772, 2685, 2762
HpySE526I ACGT 5 cut(s) 591, 985, 1126, 2982, 3291
HspAI GCGC 1 cut(s) 2472
Ksp22I TGATCA 1 cut(s) 2373
LmnI GCTCC 1 cut(s) 2437
Lsp1109I GCAGC 5 cut(s) 1407, 1997, 2691, 3039, 3275
LweI GCATC 8 cut(s) 587, 898, 1467, 1986, 2348, 2602, 2810, 3154
MaeI CTAG 9 cut(s) 35, 674, 947, 1709, 1724, 2000, 2331, 2679, 3221
MaeII ACGT 5 cut(s) 591, 985, 1126, 2982, 3291
MaeIII GTNAC 7 cut(s) 42, 238, 815, 950, 1529, 1635, 3106
MflI RGATCY 5 cut(s) 724, 1306, 1673, 1689, 2036
MhlI GDGCHC 4 cut(s) 423, 2316, 2850, 3300
MlyI GAGTC 3 cut(s) 246, 1263, 2278
MmeI TCCRAC 4 cut(s) 117, 393, 835, 1830
MroXI GAANNNNTTC 1 cut(s) 1483
MslI CAYNNNNRTG 5 cut(s) 245, 824, 1931, 2369, 2853
MspCI CTTAAG 1 cut(s) 2576
MspI CCGG 5 cut(s) 489, 927, 941, 2641, 3099
MspR9I CCNGG 3 cut(s) 1371, 2865, 3233
MssI GTTTAAAC 1 cut(s) 549
Mva1269I GAATGC 3 cut(s) 21, 1988, 2624
MvaI CCWGG 3 cut(s) 1371, 2865, 3233
MvnI CGCG 1 cut(s) 2472
MwoI GCNNNNNNNGC 4 cut(s) 119, 2903, 2976, 3177
NcoI CCATGG 1 cut(s) 1302
NdeI CATATG 2 cut(s) 1684, 2320
NlaIV GGNNCC 5 cut(s) 277, 407, 1675, 2038, 3297
NmuCI GTSAC 3 cut(s) 238, 1529, 3106
NspI RCATGY 5 cut(s) 2676, 2716, 2788, 2893, 2903
OliI CACNNNNGTG 1 cut(s) 245
PaeR7I CTCGAG 1 cut(s) 2144
PciI ACATGT 4 cut(s) 2672, 2712, 2889, 2899
PctI GAATGC 3 cut(s) 21, 1988, 2624
PdmI GAANNNNTTC 1 cut(s) 1483
PfeI GAWTC 4 cut(s) 2155, 2185, 2350, 2650
PflFI GACNNNGTC 1 cut(s) 1915
PflMI CCANNNNNTGG 3 cut(s) 1619, 2264, 2996
PfoI TCCNGGA 1 cut(s) 3231
PinAI ACCGGT 2 cut(s) 488, 926
PkrI GCNGC 7 cut(s) 785, 1422, 1778, 1987, 2706, 3054, 3265
PleI GAGTC 3 cut(s) 245, 1263, 2278
PmeI GTTTAAAC 1 cut(s) 549
PpsI GAGTC 3 cut(s) 245, 1263, 2278
Ppu21I YACGTR 2 cut(s) 1127, 2983
PscI ACATGT 4 cut(s) 2672, 2712, 2889, 2899
PshAI GACNNNNGTC 1 cut(s) 2913
Psp1406I AACGTT 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 1369, 2863, 3231
PspGI CCWGG 3 cut(s) 1369, 2863, 3231
PspN4I GGNNCC 5 cut(s) 277, 407, 1675, 2038, 3297
PspPI GGNCC 5 cut(s) 943, 1021, 1292, 2083, 2198
PstI CTGCAG 3 cut(s) 1704, 2709, 3007
PsuI RGATCY 5 cut(s) 724, 1306, 1673, 1689, 2036
PsyI GACNNNGTC 1 cut(s) 1915
RseI CAYNNNNRTG 5 cut(s) 245, 824, 1931, 2369, 2853
SatI GCNGC 7 cut(s) 784, 1421, 1777, 1986, 2705, 3053, 3264
Sau96I GGNCC 5 cut(s) 943, 1021, 1292, 2083, 2198
SchI GAGTC 3 cut(s) 246, 1263, 2278
ScrFI CCNGG 3 cut(s) 1371, 2865, 3233
SduI GDGCHC 4 cut(s) 423, 2316, 2850, 3300
SfaNI GCATC 8 cut(s) 587, 898, 1467, 1986, 2348, 2602, 2810, 3154
SfcI CTRYAG 5 cut(s) 978, 1700, 2705, 3003, 3034
Sfr274I CTCGAG 1 cut(s) 2144
SinI GGWCC 4 cut(s) 943, 1292, 2083, 2198
SlaI CTCGAG 1 cut(s) 2144
SmiMI CAYNNNNRTG 5 cut(s) 245, 824, 1931, 2369, 2853
SmlI CTYRAG 5 cut(s) 2144, 2576, 2729, 3244, 3305
SmoI CTYRAG 5 cut(s) 2144, 2576, 2729, 3244, 3305
SnaBI TACGTA 2 cut(s) 1127, 2983
SpeI ACTAGT 1 cut(s) 34
SsiI CCGC 5 cut(s) 243, 379, 783, 938, 1777
SspI AATATT 3 cut(s) 748, 1791, 1969
SspMI CTAG 9 cut(s) 35, 674, 947, 1709, 1724, 2000, 2331, 2679, 3221
StyD4I CCNGG 3 cut(s) 1369, 2863, 3231
StyI CCWWGG 3 cut(s) 946, 1302, 1708
TaaI ACNGT 8 cut(s) 160, 934, 2347, 2416, 2434, 2544, 2759, 2852
TaiI ACGT 5 cut(s) 594, 988, 1129, 2985, 3294
TaqI TCGA 9 cut(s) 456, 769, 797, 959, 1185, 1919, 2145, 2342, 2793
TaqII GACCGA 1 cut(s) 2903
TatI WGTACW 5 cut(s) 394, 685, 2408, 2529, 2709
TauI GCSGC 2 cut(s) 786, 1779
TfiI GAWTC 4 cut(s) 2155, 2185, 2350, 2650
TscAI CASTG 6 cut(s) 1894, 2352, 2439, 2847, 2855, 3088
TseFI GTSAC 3 cut(s) 238, 1529, 3106
TseI GCWGC 5 cut(s) 1420, 1985, 2704, 3052, 3263
Tsp45I GTSAC 3 cut(s) 238, 1529, 3106
TspDTI ATGAA 8 cut(s) 375, 1038, 1073, 1146, 1538, 1847, 2898, 3102
TspGWI ACGGA 1 cut(s) 2456
TspRI CASTG 6 cut(s) 1894, 2352, 2439, 2847, 2855, 3088
Tth111I GACNNNGTC 1 cut(s) 1915
Van91I CCANNNNNTGG 3 cut(s) 1619, 2264, 2996
Vha464I CTTAAG 1 cut(s) 2576
VneI GTGCAC 1 cut(s) 2846
VpaK11BI GGWCC 4 cut(s) 943, 1292, 2083, 2198
XceI RCATGY 5 cut(s) 2676, 2716, 2788, 2893, 2903
XcmI CCANNNNNNNNNTGG 2 cut(s) 1836, 2971
XhoI CTCGAG 1 cut(s) 2144
XmaJI CCTAGG 2 cut(s) 946, 1708
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 1483
XspI CTAG 9 cut(s) 35, 674, 947, 1709, 1724, 2000, 2331, 2679, 3221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.