Rh4DG176400

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0003549
Physical Location & Seq
Forward (+)
35012321 .. 35018856
6536 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG176400.1

Sequence Viewer

Length: 822 bp
ATGGAGCACTCGCAATCTCAAGATGACAGCAAAGGCAAAGCCATCAGCACCACCTTATCGGAATCCGAATGGGATGAGCCCTTCACAGAGGAAGACCTCCAAGCCATTGAAGCCGCATTCGAAGCCGCCGCGACTTCTTCTCTCTCCAAAAAGCGACGCTCCAATCCAGACAACGAAGACAGGGACCAGTCCCAGAGTGCCCGGCGCCGACTGCCGAGTTCAGTTCTCGCTCTCCAGCATCCAAACGCCTTCGCTCTCTCGCCTTGTCGTCAAGCTGCCAATATAAGGATGAGATATCCTGTGTTGAAGTTTGGAGGTCGGGTTACATATAGCAGGACTGCAGTTGAAGTGGAGAAAGCTGCCATGGAGATTTTAAAGACTGTGAAAGCAAAGAAAAACGAAATGGGTCAAAATGCAGTTGGATTTGATATTGAGTGGAAACCCACATTCCAAAAAGGTGTTCCACCTGGGAAGGTTGCAGTTCTGCAGATATGTGGAGACACAAGTTGTTGCCATGTGATGCATATCATTCATTCTGGAATTCCTCGAAGTCTGCAGTTACTTCTTGAAAGTTCTTCGATTTTGAAGGTTGGAGCTGGTATCGCTAATGATGCTGTTAAGGTTTTCAAGGATTATAATGTATCTATTAAAGCTGTGGAGGATCTTTCATATCTGGCTAATCATAAGCTTGGAGATTCACAAAGTTGGGGTCTTGCATCCCTAACTGAGAAGCTCATTTGTAAACAGGTAATCCTTGGTTTTTCTCTGCAACCGCCATTCTGCATACTTACAATTGTTATTGTTTCTGGGAAGTCTCAGTAA

Protein Analysis

273

Amino Acids

29.9

Weight (kDa)

8.46

Isoelectric Point (pI)

65.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 118 - 247 4.5e-11 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 204
AccII CGCG 1 cut(s) 131
AciI CCGC 4 cut(s) 114, 126, 129, 773
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 1 cut(s) 540
AcyI GRCGYC 1 cut(s) 205
AfiI CCNNNNNNNGG 1 cut(s) 285
AgsI TTSAA 6 cut(s) 110, 307, 347, 569, 586, 628
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 6 cut(s) 275, 359, 596, 653, 688, 733
AluI AGCT 6 cut(s) 275, 359, 596, 653, 688, 733
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 492
AlwI GGATC 1 cut(s) 669
ApeKI GCWGC 2 cut(s) 275, 359
ApoI RAATTY 1 cut(s) 540
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 202
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 2 cut(s) 99, 183
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 2 cut(s) 262, 346
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 492
BfmI CTRYAG 3 cut(s) 339, 485, 554
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 5 cut(s) 114, 126, 129, 276, 360
BlsI GCNGC 5 cut(s) 115, 127, 130, 277, 361
Bme1390I CCNGG 2 cut(s) 202, 468
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 2 cut(s) 185, 206
BmrFI CCNGG 2 cut(s) 202, 468
BmsI GCATC 4 cut(s) 247, 510, 601, 725
BpiI GAAGAC 2 cut(s) 99, 183
BpmI CTGGAG 1 cut(s) 218
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 205
BsaJI CCNNGG 3 cut(s) 363, 467, 754
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 285
Bse1I ACTGG 1 cut(s) 187
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 3 cut(s) 363, 467, 754
BseGI GGATG 4 cut(s) 79, 238, 294, 716
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 717
BseNI ACTGG 1 cut(s) 187
BseSI GKGCMC 1 cut(s) 202
BseXI GCAGC 2 cut(s) 262, 346
Bsh1236I CGCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 2 cut(s) 175, 197
BslI CCNNNNNNNGG 1 cut(s) 285
BsmAI GTCTC 1 cut(s) 492
BsmFI GGGAC 2 cut(s) 175, 197
BsmI GAATGC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 9, 81, 202
Bsp143I GATC 1 cut(s) 661
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 4 cut(s) 114, 126, 129, 773
BspCNI CTCAG 1 cut(s) 718
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 185, 206
BspMAI CTGCAG 3 cut(s) 343, 489, 558
BspPI GGATC 1 cut(s) 669
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 187
BssECI CCNNGG 3 cut(s) 363, 467, 754
BssMI GATC 1 cut(s) 661
BssNI GRCGYC 1 cut(s) 205
BssT1I CCWWGG 2 cut(s) 363, 754
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 382
BstACI GRCGYC 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 726, 816
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 4 cut(s) 79, 238, 294, 716
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 1 cut(s) 492
BstMBI GATC 1 cut(s) 661
BstMWI GCNNNNNNNGC 5 cut(s) 110, 122, 211, 602, 611
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 200, 466
BstSFI CTRYAG 3 cut(s) 339, 485, 554
BstSLI GKGCMC 1 cut(s) 202
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 2 cut(s) 262, 346
BstV2I GAAGAC 2 cut(s) 99, 183
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 4 cut(s) 79, 238, 294, 716
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 1 cut(s) 165
CviAII CATG 2 cut(s) 364, 515
DdeI CTNAG 2 cut(s) 726, 816
DinI GGCGCC 1 cut(s) 206
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
DraI TTTAAA 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 363, 754
Eco24I GRGCYC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 2 cut(s) 363, 754
EcoT22I ATGCAT 1 cut(s) 525
EcoT38I GRGCYC 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
ErhI CCWWGG 2 cut(s) 363, 754
FaeI CATG 2 cut(s) 367, 518
FaqI GGGAC 2 cut(s) 175, 197
FatI CATG 2 cut(s) 363, 514
Fnu4HI GCNGC 5 cut(s) 114, 126, 129, 276, 360
FokI GGATG 4 cut(s) 86, 225, 301, 703
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 5 cut(s) 114, 126, 129, 276, 360
GlaI GCGC 1 cut(s) 206
GluI GCNGC 5 cut(s) 114, 126, 129, 276, 360
GsuI CTGGAG 1 cut(s) 218
HaeII RGCGCY 1 cut(s) 208
HapII CCGG 1 cut(s) 202
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 2 cut(s) 367, 518
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 2 cut(s) 62, 695
HpaII CCGG 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 743
Hpy188I TCNGA 2 cut(s) 61, 67
Hpy188III TCNNGA 4 cut(s) 20, 167, 537, 566
Hpy8I GTNNAC 1 cut(s) 743
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 4 cut(s) 91, 259, 466, 580
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4V TGCA 9 cut(s) 341, 416, 479, 487, 523, 556, 716, 769, 783
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 122, 211, 602, 611
HpyF3I CTNAG 2 cut(s) 726, 816
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 2 cut(s) 367, 518
HspAI GCGC 1 cut(s) 205
KasI GGCGCC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 661
LmnI GCTCC 3 cut(s) 4, 164, 593
Lsp1109I GCAGC 2 cut(s) 262, 346
LweI GCATC 4 cut(s) 247, 510, 601, 725
MaeIII GTNAC 2 cut(s) 322, 558
MalI GATC 1 cut(s) 663
MboI GATC 1 cut(s) 661
MboII GAAGA 4 cut(s) 104, 129, 188, 567
MfeI CAATTG 1 cut(s) 792
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 3 cut(s) 9, 81, 202
MluCI AATT 2 cut(s) 540, 792
Mly113I GGCGCC 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 400, 571
MnlI CCTC 5 cut(s) 82, 107, 308, 555, 652
Mph1103I ATGCAT 1 cut(s) 525
MseI TTAA 3 cut(s) 374, 618, 648
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 202, 468
MunI CAATTG 1 cut(s) 792
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 5 cut(s) 110, 122, 211, 602, 611
NarI GGCGCC 1 cut(s) 205
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 363
NdeII GATC 1 cut(s) 661
NlaIII CATG 2 cut(s) 367, 518
NlaIV GGNNCC 2 cut(s) 185, 206
NmeAIII GCCGAG 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 525
NspV TTCGAA 1 cut(s) 120
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 62, 695
PkrI GCNGC 5 cut(s) 115, 127, 130, 277, 361
PluTI GGCGCC 1 cut(s) 208
PsiI TTATAA 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 185, 206
PspPI GGNCC 1 cut(s) 184
PstI CTGCAG 3 cut(s) 343, 489, 558
PsuI RGATCY 1 cut(s) 661
SaqAI TTAA 3 cut(s) 374, 618, 648
SatI GCNGC 5 cut(s) 114, 126, 129, 276, 360
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 202, 468
SduI GDGCHC 3 cut(s) 9, 81, 202
SfaNI GCATC 4 cut(s) 247, 510, 601, 725
SfcI CTRYAG 3 cut(s) 339, 485, 554
SfoI GGCGCC 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 2 cut(s) 540, 792
SsiI CCGC 4 cut(s) 114, 126, 129, 773
SspDI GGCGCC 1 cut(s) 204
StyD4I CCNGG 2 cut(s) 200, 466
StyI CCWWGG 2 cut(s) 363, 754
TaaI ACNGT 1 cut(s) 382
TaqI TCGA 3 cut(s) 120, 547, 578
TasI AATT 2 cut(s) 540, 792
TauI GCSGC 3 cut(s) 116, 128, 131
TfiI GAWTC 2 cut(s) 62, 695
Tru1I TTAA 3 cut(s) 374, 618, 648
Tru9I TTAA 3 cut(s) 374, 618, 648
TseI GCWGC 2 cut(s) 275, 359
TspDTI ATGAA 2 cut(s) 521, 657
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 1 cut(s) 540
Zsp2I ATGCAT 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.