Rw4G015130

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0003549
Physical Location & Seq
Forward (+)
34842394 .. 34847257
4864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G015130.1

Sequence Viewer

Length: 996 bp
ATGGAGCACTCGCAATCTCAAGATGACAGCAAAGGCAAAGCCATCAGCACCACCTTATCGGAATCCGAATGGGATGAGCCCTTCACAGAGGAAGACCTCCAAGCCATTGAAGCCGCATTCGAAGCCGCCGCGACTTCTTCTCTCTCCAAAAAGCGACGCTCCAATCCAGACAACGAAGACAGGGACCAGTCCCAGAGTGCCCGGCGCCGACTGCCGAGTTCAGTTCTCGCTCTCCAGCATCCAAACGCCTTCGCTCTCTCGCCTTGTCGTCAAGCTGCCAATATAAGGATGAGATATCCTGTGTTGAAGTTTGGAGGTCGGGTTACATATAGCAGGACTGCAGTTGAAGTGGAGAAAGCTGCCATGGAGATTTTAAAGACTGTGAAAGCAAAGAAAAACGAAATGGGTCAAAATGCAGTTGGATTTGATATTGAGTGGAAACCCACATTCCAAAGAGGTGTTCCACCTGGGAAGGTTGCAGTTCTGCAGATATGTGGAGACACAAGTTGTTGCCATGTGATGCATATCATTCATTCTGGAATTCCTCGAAGTCTGCAGTTACTTCTTGAAAGTTCTTCGATTTTGAAGGTTGGAGCTGGTATCGCTAATGATGCTGTTAAGGTTTTCAAGGATTATAATGTATCTATTAAAGCTGTGGAGGATCTTTCATATCTGGCTAATCATAAGCTTGGAGATTCACAAAATTGGGGTCTTGCATCCCTAACTGAGAAGCTCATTTGTAAACAGCTTCTAAAGCCCAAAAAAATTCGATTGGGTAACTGGGAGACCAAATATTTATCAAAAGAGCAGCTGCAGTATGCTGCCACTGATGCATTTGCATCATGGTATCTTCATGAGGTGCTAAGGAGCCTCCCTGATGCTGAAAAACTTGAAAAAGTTACAGCAGACAACCAGAGTGAGGGAAAGCCGTATCATGTAATTACTTTCAGACTGAGGAACCACAAGCTGCATCATTGTAATGTCTTTCATTGGTAA

Protein Analysis

331

Amino Acids

37.19

Weight (kDa)

8.87

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 118 - 289 4.8e-21 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AccB1I GGYRCC 1 cut(s) 204
AccII CGCG 1 cut(s) 131
AciI CCGC 3 cut(s) 114, 126, 129
AclWI GGATC 1 cut(s) 669
AcsI RAATTY 2 cut(s) 540, 765
AcyI GRCGYC 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 285, 919
AgsI TTSAA 7 cut(s) 110, 307, 347, 569, 586, 628, 893
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AluBI AGCT 9 cut(s) 275, 359, 596, 653, 688, 733, 748, 811, 967
AluI AGCT 9 cut(s) 275, 359, 596, 653, 688, 733, 748, 811, 967
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 492, 779
AlwI GGATC 1 cut(s) 669
ApeKI GCWGC 6 cut(s) 275, 359, 808, 811, 821, 967
ApoI RAATTY 2 cut(s) 540, 765
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 202
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 1 cut(s) 184
BaeGI GKGCMC 1 cut(s) 202
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 2 cut(s) 99, 183
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 6 cut(s) 262, 346, 798, 808, 820, 954
BccI CCATC 1 cut(s) 50
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 1 cut(s) 468
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 492, 779
BfmI CTRYAG 4 cut(s) 339, 485, 554, 812
BfoI RGCGCY 1 cut(s) 208
BisI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 968
BlsI GCNGC 9 cut(s) 115, 127, 130, 277, 361, 810, 813, 823, 969
Bme1390I CCNGG 2 cut(s) 202, 468
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmiI GGNNCC 4 cut(s) 185, 206, 869, 959
BmrFI CCNGG 2 cut(s) 202, 468
BmrI ACTGGG 1 cut(s) 790
BmsI GCATC 8 cut(s) 247, 510, 601, 725, 820, 848, 868, 979
BmuI ACTGGG 1 cut(s) 790
BpiI GAAGAC 2 cut(s) 99, 183
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 863
Bpu14I TTCGAA 1 cut(s) 120
BpuMI CCSGG 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 524
BsaHI GRCGYC 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 779
BsaJI CCNNGG 2 cut(s) 363, 467
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 919
Bse1I ACTGG 2 cut(s) 187, 785
Bse8I GATNNNNATC 1 cut(s) 524
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 363, 467
BseGI GGATG 4 cut(s) 79, 238, 294, 716
BseJI GATNNNNATC 1 cut(s) 524
BseLI CCNNNNNNNGG 2 cut(s) 285, 919
BseMII CTCAG 2 cut(s) 717, 944
BseNI ACTGG 2 cut(s) 187, 785
BseSI GKGCMC 1 cut(s) 202
BseXI GCAGC 6 cut(s) 262, 346, 798, 808, 820, 954
Bsh1236I CGCG 1 cut(s) 131
BshNI GGYRCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 2 cut(s) 175, 197
BslI CCNNNNNNNGG 2 cut(s) 285, 919
BsmAI GTCTC 2 cut(s) 492, 779
BsmFI GGGAC 2 cut(s) 175, 197
BsmI GAATGC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 779
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 9, 81, 202
Bsp143I GATC 1 cut(s) 661
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 3 cut(s) 114, 126, 129
BspCNI CTCAG 2 cut(s) 718, 945
BspFNI CGCG 1 cut(s) 131
BspHI TCATGA 1 cut(s) 853
BspLI GGNNCC 4 cut(s) 185, 206, 869, 959
BspMAI CTGCAG 4 cut(s) 343, 489, 558, 816
BspPI GGATC 1 cut(s) 669
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 204
BspTNI GGTCTC 1 cut(s) 779
BsrI ACTGG 2 cut(s) 187, 785
BssECI CCNNGG 2 cut(s) 363, 467
BssMI GATC 1 cut(s) 661
BssNI GRCGYC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 1 cut(s) 382
BstACI GRCGYC 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 120
BstDEI CTNAG 3 cut(s) 726, 863, 953
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 4 cut(s) 79, 238, 294, 716
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 1 cut(s) 664
BstMAI GTCTC 2 cut(s) 492, 779
BstMBI GATC 1 cut(s) 661
BstMWI GCNNNNNNNGC 7 cut(s) 110, 122, 211, 602, 611, 754, 830
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 2 cut(s) 200, 466
BstSFI CTRYAG 4 cut(s) 339, 485, 554, 812
BstSLI GKGCMC 1 cut(s) 202
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 6 cut(s) 262, 346, 798, 808, 820, 954
BstV2I GAAGAC 2 cut(s) 99, 183
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 4 cut(s) 79, 238, 294, 716
BtsIMutI CAGTG 1 cut(s) 825
CciI TCATGA 1 cut(s) 853
CfoI GCGC 1 cut(s) 207
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 1 cut(s) 165
CviAII CATG 5 cut(s) 364, 515, 843, 854, 935
DdeI CTNAG 3 cut(s) 726, 863, 953
DinI GGCGCC 1 cut(s) 206
DpnI GATC 1 cut(s) 663
DpnII GATC 1 cut(s) 661
DraI TTTAAA 1 cut(s) 375
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 779
Eco32I GATATC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 184
EcoRI GAATTC 1 cut(s) 540
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 363
EcoT22I ATGCAT 2 cut(s) 525, 835
EcoT38I GRGCYC 1 cut(s) 81
EgeI GGCGCC 1 cut(s) 206
EheI GGCGCC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 5 cut(s) 367, 518, 846, 857, 938
FaqI GGGAC 2 cut(s) 175, 197
FatI CATG 5 cut(s) 363, 514, 842, 853, 934
Fnu4HI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 968
FokI GGATG 4 cut(s) 86, 225, 301, 703
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 968
GlaI GCGC 1 cut(s) 206
GluI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 968
GsuI CTGGAG 1 cut(s) 218
HaeII RGCGCY 1 cut(s) 208
HapII CCGG 1 cut(s) 202
HgaI GACGC 1 cut(s) 165
HhaI GCGC 1 cut(s) 207
Hin1I GRCGYC 1 cut(s) 205
Hin1II CATG 5 cut(s) 367, 518, 846, 857, 938
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 2 cut(s) 62, 695
HpaII CCGG 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 743
Hpy188I TCNGA 3 cut(s) 61, 67, 950
Hpy188III TCNNGA 5 cut(s) 20, 167, 537, 566, 854
Hpy8I GTNNAC 1 cut(s) 743
Hpy99I CGWCG 1 cut(s) 159
HpyAV CCTTC 4 cut(s) 91, 259, 466, 580
HpyCH4III ACNGT 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 122, 211, 602, 611, 754, 830
HpyF3I CTNAG 3 cut(s) 726, 863, 953
Hsp92I GRCGYC 1 cut(s) 205
Hsp92II CATG 5 cut(s) 367, 518, 846, 857, 938
HspAI GCGC 1 cut(s) 205
KasI GGCGCC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 661
LmnI GCTCC 4 cut(s) 4, 164, 593, 867
Lsp1109I GCAGC 6 cut(s) 262, 346, 798, 808, 820, 954
LweI GCATC 8 cut(s) 247, 510, 601, 725, 820, 848, 868, 979
MaeIII GTNAC 4 cut(s) 322, 558, 776, 898
MalI GATC 1 cut(s) 663
MboI GATC 1 cut(s) 661
MboII GAAGA 5 cut(s) 104, 129, 188, 567, 842
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 3 cut(s) 9, 81, 202
MluCI AATT 4 cut(s) 540, 703, 765, 939
Mly113I GGCGCC 1 cut(s) 205
MmeI TCCRAC 2 cut(s) 400, 571
Mph1103I ATGCAT 2 cut(s) 525, 835
MseI TTAA 3 cut(s) 374, 618, 648
MslI CAYNNNNRTG 1 cut(s) 978
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 202, 468
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 468
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 110, 122, 211, 602, 611, 754, 830
NarI GGCGCC 1 cut(s) 205
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 363
NdeII GATC 1 cut(s) 661
NlaIII CATG 5 cut(s) 367, 518, 846, 857, 938
NlaIV GGNNCC 4 cut(s) 185, 206, 869, 959
NmeAIII GCCGAG 1 cut(s) 240
NsiI ATGCAT 2 cut(s) 525, 835
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 1 cut(s) 853
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 62, 695
PkrI GCNGC 9 cut(s) 115, 127, 130, 277, 361, 810, 813, 823, 969
PluTI GGCGCC 1 cut(s) 208
PsiI TTATAA 1 cut(s) 636
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 4 cut(s) 185, 206, 869, 959
PspPI GGNCC 1 cut(s) 184
PstI CTGCAG 4 cut(s) 343, 489, 558, 816
PsuI RGATCY 1 cut(s) 661
PvuII CAGCTG 1 cut(s) 811
RseI CAYNNNNRTG 1 cut(s) 978
SaqAI TTAA 3 cut(s) 374, 618, 648
SatI GCNGC 9 cut(s) 114, 126, 129, 276, 360, 809, 812, 822, 968
Sau3AI GATC 1 cut(s) 661
Sau96I GGNCC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 202, 468
SduI GDGCHC 3 cut(s) 9, 81, 202
SfaNI GCATC 8 cut(s) 247, 510, 601, 725, 820, 848, 868, 979
SfcI CTRYAG 4 cut(s) 339, 485, 554, 812
SfoI GGCGCC 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 1 cut(s) 184
SmiMI CAYNNNNRTG 1 cut(s) 978
SmlI CTYRAG 1 cut(s) 18
SmoI CTYRAG 1 cut(s) 18
Sse9I AATT 4 cut(s) 540, 703, 765, 939
SsiI CCGC 3 cut(s) 114, 126, 129
SspDI GGCGCC 1 cut(s) 204
SspI AATATT 1 cut(s) 794
StyD4I CCNGG 2 cut(s) 200, 466
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 382
TaqI TCGA 4 cut(s) 120, 547, 578, 769
TasI AATT 4 cut(s) 540, 703, 765, 939
TauI GCSGC 3 cut(s) 116, 128, 131
TfiI GAWTC 2 cut(s) 62, 695
Tru1I TTAA 3 cut(s) 374, 618, 648
Tru9I TTAA 3 cut(s) 374, 618, 648
TscAI CASTG 1 cut(s) 832
TseI GCWGC 6 cut(s) 275, 359, 808, 811, 821, 967
TspDTI ATGAA 4 cut(s) 521, 657, 842, 977
TspRI CASTG 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 184
XapI RAATTY 2 cut(s) 540, 765
Zsp2I ATGCAT 2 cut(s) 525, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.