AT4G14220

RING-type zinc-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012488
Physical Location & Seq
Forward (+)
8195881 .. 8198586
2706 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14220.1

Sequence Viewer

Length: 1116 bp
ATGTCTAATTTCACTTATACATCTGCTTTCAACTTATCCGACAACTCCCCTTTTAATCCCGCCATTGGTTCTTCGTCTTCTTCTTCCTCCGCGCTCGTCGTAGCTTCCGATGATGATAATAATACCGATGATGCTTGTAGCATCTGTCTCGAGCCTTTCACCCTCCAGGATCCTTCCACTGTCACCAGCTGCAAACATGAATATCACCTTCAGTGCATCATTGAATGGTCACAGAGAAGCAAAGAGTGTCCTATTTGCTGGCAATTGTTTGTTTTGAGAGACCCTGCTAGCCAAGAGCTTTTAGCTGCTGTGGAAAAGGAAAGACTTTTGAAAACCAGGAATATATCTTCATCCTCTCCAATCTCTATTCATCACTCTCATGACGATTTTCACTCTGAGGAGGAGGAATCACAGTTTAGCAGCTTTGATGAACAATTCTTGAGGCATCTTACTGAAGCTGCACATAGACGTTGTTTGCTTCGCAGAAGGGATGGTCAAATATCCTCTAGTCTTGTTTCGTCCAGCGATCCCACTACGATTCATCCTACTGATTTGGTCAATCTTTATCGTCTCTCTGCTATTTCCCATGTTGAACATCAAAACTCAAACCCTTGTCCAAGTCCTGGCTCAATGACTCCATCTCCCGTCTCTGGTCATTCTTCCATTCCTGCAGATAGCAATAACGGATCCAGAATCTCTCCTGGCCCTTCTCCATCTCGTTCATCTCAAAGTCCAAAATCACCTGAAGCATCCTCGCTTCCGGAAGCCATTAAATCGAAACTAGCTGCTGCTTCTGCAAAGTACAAGGAATCGATCTCTAAAAGTAAACAAGGTCTTAAAGAGAAGCTACTTGCTCGCAATAACTCAGTGAAAGAATTGAGCAAAGGAGTGCAACGTGAGATGAATGCAGGAATAGCTGGTGTTGCACGAATGATCGAACGCATGGATTTTTCTTCAAAACGTTTTGGAGGGTCTGCTCATGTATCAACCTCCACTGCTACTGCTTCTGGATTCAATTTCTCATTCAAAGGGAAACGTGTAGAAGCCAATTCTAAGTCTAACAACAATGGAGACAAAACCGAACCCCAAAAGCTGCAGGGAGGAGAAACGTGCTGA

Protein Analysis

371

Amino Acids

40.28

Weight (kDa)

6.89

Isoelectric Point (pI)

67.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 40 - 86 1.1e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 45 - 86 7.7e-10 Ring finger domain
zf-RING_11 PF17123 46 - 73 9.5e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 46 - 86 4.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 46 - 79 6.5e-07 RING-type zinc-finger
zf-C3HC4 PF00097 46 - 86 1.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14220
fragaria_vesca FvH4_4g14860
malus_domestica MD13G1185900.v1.1 MD16G1186700.v1.1
prunus_persica Prupe.1G153500_v2.0.a1
pyrus_communis pycom13g16040 pycom16g15650
rosa_chinensis RchiOBHm_Chr4g0417351 RchiOBHm_Chr4g0417381
rosa_laevigata RLG00000007915
rosa_multiflora Rmu_co8127690.1_g000001 Rmu_sc0004142.1_g000031
rosa_roxburghii Rroxscaffold_5G00361110
rosa_rugosa Rorug04G0147200
rosa_samantha Rh4AG210700 Rh4BG211200 Rh4CG221600 Rh4DG208600
rosa_wichuraiana Rw4G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 623
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 760
AciI CCGC 2 cut(s) 60, 90
AclI AACGTT 1 cut(s) 961
AclWI GGATC 5 cut(s) 164, 177, 521, 681, 694
AcuI CTGAAG 3 cut(s) 194, 474, 765
AfaI GTAC 1 cut(s) 803
AfiI CCNNNNNNNGG 3 cut(s) 65, 623, 650
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 7 cut(s) 31, 224, 331, 593, 957, 1015, 1027
AjnI CCWGG 4 cut(s) 165, 335, 622, 700
Alw26I GTCTC 5 cut(s) 152, 273, 575, 652, 1065
AlwI GGATC 5 cut(s) 164, 177, 521, 681, 694
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 760
AoxI GGCC 1 cut(s) 703
ApeKI GCWGC 7 cut(s) 189, 305, 420, 458, 785, 788, 1093
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 704
AsuHPI GGTGA 4 cut(s) 151, 175, 197, 732
AsuNHI GCTAGC 1 cut(s) 287
AvaI CYCGRG 1 cut(s) 149
BamHI GGATCC 2 cut(s) 169, 686
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 7 cut(s) 176, 292, 432, 445, 772, 775, 1080
BccI CCATC 3 cut(s) 485, 646, 721
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 4 cut(s) 167, 337, 624, 702
BcoDI GTCTC 5 cut(s) 152, 273, 575, 652, 1065
BfaI CTAG 3 cut(s) 288, 507, 782
BfmI CTRYAG 2 cut(s) 669, 1094
BisI GCNGC 7 cut(s) 190, 306, 421, 459, 786, 789, 1094
BlsI GCNGC 7 cut(s) 191, 307, 422, 460, 787, 790, 1095
Bme1390I CCNGG 4 cut(s) 167, 337, 624, 702
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 2 cut(s) 171, 688
BmrFI CCNGG 4 cut(s) 167, 337, 624, 702
BmsI GCATC 5 cut(s) 121, 150, 225, 454, 758
BmtI GCTAGC 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 149
BpuEI CTTGAG 1 cut(s) 460
Bsa29I ATCGAT 1 cut(s) 812
BsaI GGTCTC 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 760
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 623, 650
BseAI TCCGGA 1 cut(s) 760
BseBI CCWGG 4 cut(s) 167, 337, 624, 702
BseCI ATCGAT 1 cut(s) 812
BseGI GGATG 4 cut(s) 350, 496, 541, 749
BseLI CCNNNNNNNGG 3 cut(s) 65, 623, 650
BseMII CTCAG 2 cut(s) 387, 879
BseRI GAGGAG 3 cut(s) 413, 416, 1116
BseXI GCAGC 7 cut(s) 176, 292, 432, 445, 772, 775, 1080
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 1 cut(s) 705
BshVI ATCGAT 1 cut(s) 812
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 1 cut(s) 761
BslI CCNNNNNNNGG 3 cut(s) 65, 623, 650
BsmAI GTCTC 5 cut(s) 152, 273, 575, 652, 1065
BsmBI CGTCTC 2 cut(s) 575, 652
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 1 cut(s) 705
Bso31I GGTCTC 1 cut(s) 273
BsoBI CYCGRG 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 760
Bsp143I GATC 5 cut(s) 169, 526, 686, 813, 933
BspACI CCGC 2 cut(s) 60, 90
BspANI GGCC 1 cut(s) 705
BspCNI CTCAG 2 cut(s) 388, 878
BspDI ATCGAT 1 cut(s) 812
BspEI TCCGGA 1 cut(s) 760
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 171, 688
BspMAI CTGCAG 2 cut(s) 673, 1098
BspOI GCTAGC 1 cut(s) 291
BspPI GGATC 5 cut(s) 164, 177, 521, 681, 694
BspTNI GGTCTC 1 cut(s) 273
BssMI GATC 5 cut(s) 169, 526, 686, 813, 933
Bst2UI CCWGG 4 cut(s) 167, 337, 624, 702
Bst4CI ACNGT 2 cut(s) 181, 414
BstC8I GCNNGC 3 cut(s) 260, 289, 856
BstDEI CTNAG 3 cut(s) 396, 865, 1053
BstF5I GGATG 4 cut(s) 350, 496, 541, 749
BstFNI CGCG 1 cut(s) 92
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 5 cut(s) 172, 529, 689, 816, 936
BstMAI GTCTC 5 cut(s) 152, 273, 575, 652, 1065
BstMBI GATC 5 cut(s) 169, 526, 686, 813, 933
BstMWI GCNNNNNNNGC 3 cut(s) 794, 914, 923
BstNI CCWGG 4 cut(s) 167, 337, 624, 702
BstSCI CCNGG 4 cut(s) 165, 335, 622, 700
BstSFI CTRYAG 2 cut(s) 669, 1094
BstUI CGCG 1 cut(s) 92
BstV1I GCAGC 7 cut(s) 176, 292, 432, 445, 772, 775, 1080
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 169, 686
BstYI RGATCY 2 cut(s) 169, 686
Bsu15I ATCGAT 1 cut(s) 812
BsuRI GGCC 1 cut(s) 705
BsuTUI ATCGAT 1 cut(s) 812
BtsCI GGATG 4 cut(s) 350, 496, 541, 749
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 4 cut(s) 177, 218, 873, 993
Cac8I GCNNGC 3 cut(s) 260, 289, 856
CciI TCATGA 1 cut(s) 379
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 704
ClaI ATCGAT 1 cut(s) 812
Csp6I GTAC 1 cut(s) 802
CviAII CATG 5 cut(s) 197, 380, 587, 943, 980
CviQI GTAC 1 cut(s) 802
DdeI CTNAG 3 cut(s) 396, 865, 1053
DpnI GATC 5 cut(s) 171, 528, 688, 815, 935
DpnII GATC 5 cut(s) 169, 526, 686, 813, 933
Eco31I GGTCTC 1 cut(s) 273
Eco57I CTGAAG 3 cut(s) 194, 474, 765
Eco88I CYCGRG 1 cut(s) 149
EcoRII CCWGG 4 cut(s) 165, 335, 622, 700
Esp3I CGTCTC 2 cut(s) 575, 652
FaeI CATG 5 cut(s) 200, 383, 590, 946, 983
FaiI YATR 8 cut(s) 18, 198, 344, 381, 465, 588, 944, 981
FatI CATG 5 cut(s) 196, 379, 586, 942, 979
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 7 cut(s) 190, 306, 421, 459, 786, 789, 1094
FokI GGATG 4 cut(s) 337, 503, 528, 736
Fsp4HI GCNGC 7 cut(s) 190, 306, 421, 459, 786, 789, 1094
FspBI CTAG 3 cut(s) 288, 507, 782
GlaI GCGC 1 cut(s) 93
GluI GCNGC 7 cut(s) 190, 306, 421, 459, 786, 789, 1094
GsuI CTGGAG 1 cut(s) 149
HaeIII GGCC 1 cut(s) 705
HapII CCGG 1 cut(s) 761
HhaI GCGC 1 cut(s) 94
Hin1II CATG 5 cut(s) 200, 383, 590, 946, 983
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 6 cut(s) 407, 538, 634, 693, 809, 1011
HpaII CCGG 1 cut(s) 761
HphI GGTGA 4 cut(s) 151, 175, 197, 732
Hpy166II GTNNAC 1 cut(s) 827
Hpy188I TCNGA 3 cut(s) 40, 109, 397
Hpy188III TCNNGA 6 cut(s) 149, 380, 439, 690, 761, 1008
Hpy8I GTNNAC 1 cut(s) 827
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 4 cut(s) 183, 218, 480, 717
HpyCH4III ACNGT 2 cut(s) 181, 414
HpyCH4IV ACGT 5 cut(s) 469, 895, 961, 1036, 1109
HpyCH4V TGCA 9 cut(s) 192, 216, 461, 671, 797, 892, 908, 926, 1096
HpyF10VI GCNNNNNNNGC 3 cut(s) 794, 914, 923
HpyF3I CTNAG 3 cut(s) 396, 865, 1053
HpySE526I ACGT 5 cut(s) 469, 895, 961, 1036, 1109
Hsp92II CATG 5 cut(s) 200, 383, 590, 946, 983
HspAI GCGC 1 cut(s) 92
Kpn2I TCCGGA 1 cut(s) 760
Kzo9I GATC 5 cut(s) 169, 526, 686, 813, 933
Lsp1109I GCAGC 7 cut(s) 176, 292, 432, 445, 772, 775, 1080
LweI GCATC 5 cut(s) 121, 150, 225, 454, 758
MaeI CTAG 3 cut(s) 288, 507, 782
MaeII ACGT 5 cut(s) 469, 895, 961, 1036, 1109
MaeIII GTNAC 2 cut(s) 181, 228
MalI GATC 5 cut(s) 171, 528, 688, 815, 935
MboI GATC 5 cut(s) 169, 526, 686, 813, 933
MboII GAAGA 7 cut(s) 63, 69, 72, 75, 339, 651, 945
MfeI CAATTG 1 cut(s) 263
MflI RGATCY 2 cut(s) 169, 686
MluCI AATT 6 cut(s) 7, 263, 434, 875, 1015, 1048
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 1 cut(s) 63
MroI TCCGGA 1 cut(s) 760
MseI TTAA 3 cut(s) 54, 771, 837
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 189
MspI CCGG 1 cut(s) 761
MspR9I CCNGG 4 cut(s) 167, 337, 624, 702
MunI CAATTG 1 cut(s) 263
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 4 cut(s) 167, 337, 624, 702
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 3 cut(s) 794, 914, 923
NdeII GATC 5 cut(s) 169, 526, 686, 813, 933
NheI GCTAGC 1 cut(s) 287
NlaIII CATG 5 cut(s) 200, 383, 590, 946, 983
NlaIV GGNNCC 2 cut(s) 171, 688
NmuCI GTSAC 2 cut(s) 181, 228
PaeR7I CTCGAG 1 cut(s) 149
PagI TCATGA 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 5 cut(s) 407, 538, 693, 809, 1011
PflMI CCANNNNNTGG 1 cut(s) 623
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 7 cut(s) 191, 307, 422, 460, 787, 790, 1095
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Psp1406I AACGTT 1 cut(s) 961
Psp6I CCWGG 4 cut(s) 165, 335, 622, 700
PspGI CCWGG 4 cut(s) 165, 335, 622, 700
PspN4I GGNNCC 2 cut(s) 171, 688
PspPI GGNCC 1 cut(s) 704
PstI CTGCAG 2 cut(s) 673, 1098
PsuI RGATCY 2 cut(s) 169, 686
PvuII CAGCTG 1 cut(s) 189
RsaI GTAC 1 cut(s) 803
RsaNI GTAC 1 cut(s) 802
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 54, 771, 837
SatI GCNGC 7 cut(s) 190, 306, 421, 459, 786, 789, 1094
Sau3AI GATC 5 cut(s) 169, 526, 686, 813, 933
Sau96I GGNCC 1 cut(s) 704
SchI GAGTC 1 cut(s) 628
ScrFI CCNGG 4 cut(s) 167, 337, 624, 702
SfaNI GCATC 5 cut(s) 121, 150, 225, 454, 758
SfcI CTRYAG 2 cut(s) 669, 1094
Sfr274I CTCGAG 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 149
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 2 cut(s) 149, 439
SmoI CTYRAG 2 cut(s) 149, 439
Sse9I AATT 6 cut(s) 7, 263, 434, 875, 1015, 1048
SsiI CCGC 2 cut(s) 60, 90
SspMI CTAG 3 cut(s) 288, 507, 782
StyD4I CCNGG 4 cut(s) 165, 335, 622, 700
TaaI ACNGT 2 cut(s) 181, 414
TaiI ACGT 5 cut(s) 472, 898, 964, 1039, 1112
TaqI TCGA 4 cut(s) 150, 776, 812, 936
TasI AATT 6 cut(s) 7, 263, 434, 875, 1015, 1048
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 5 cut(s) 407, 538, 693, 809, 1011
Tru1I TTAA 3 cut(s) 54, 771, 837
Tru9I TTAA 3 cut(s) 54, 771, 837
TscAI CASTG 4 cut(s) 184, 218, 873, 1000
TseFI GTSAC 2 cut(s) 181, 228
TseI GCWGC 7 cut(s) 189, 305, 420, 458, 785, 788, 1093
Tsp45I GTSAC 2 cut(s) 181, 228
TspDTI ATGAA 7 cut(s) 213, 339, 359, 444, 530, 711, 917
TspGWI ACGGA 1 cut(s) 699
TspRI CASTG 4 cut(s) 184, 218, 873, 1000
Van91I CCANNNNNTGG 1 cut(s) 623
XhoI CTCGAG 1 cut(s) 149
XspI CTAG 3 cut(s) 288, 507, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.