RchiOBHm_Chr4g0417351

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012488
Physical Location & Seq
Forward (+)
42598831 .. 42601937
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38744

Sequence Viewer

Length: 1134 bp
ATGGCATCTTTCGCTCCTTCCTCTTCTTCTTCTTCTGATAACCCAATACCCATCTGGACTCCATCCTCCTCTGTATCTGCTTTTCCCGACGAGCCGTATGAAGACTCCTGCAGTATCTGCCTCGAGCCTTTCTGCTCCGATGACCCAGCCACCATTACCAGCTGCAAACATGACTATCATCTCCACTGTATATTAGAATGGTCACAAAGAAGTAAAGAATGCCCAATTTGTTGGCAGTTACTTGTCTTGAAGGATCCGGCTAGCCAAGGGCTTCTAGATGTTGTACAGAATGAGAAGCGCTCAAGGGCAAGAAACATATCTTCCATTGCCCCCCCAACTTTTCATAATTCCTCTGAGGACTTTGACTTTGAACATGATTCGTTCTCAGATGACTCCGATTTGGACGAGCGTATTATGCAGCATCTTGCTGCTGCGTCTAGCAGGGCTCGTTATGTACGCAGAAGGGAAAGACAGAGATATTCTGGACTGGGCCCTTCCAATGGTTTTAATTTTTCCCCTCGTGAAAATGTTTCTGGTATGGAGCAAGCACACCCAACTTCTCCTGTAGAACGTCCAAGTTTCACCTATGGGTCACCAGGAGGTGATTCACCAACTTCTCCAAGACCACCTGTTGTTCAACCTCCACCATCTTTTATCTGCAGCACTGCTGCCAACAATGATCCTTTCAAACCAAGAGTTCTCTTTGGCCAGCCAACACCTAATGGACCACAAAGACAAAGCCCATCTGAGATGCTCAATTTTCCCGAGTCAATCAAATCAAAGCTGTCTGCTGCTTCAGCCAGATACAAAGAGTCCATCTCCAAAGGCACCCGAGGGTTGAAGGAAAAGCTGCTTGCTCGTAACAACTCAGTCAAGGAGATGAGCAAAGGAGTTCAACGTGAGATGACTGCAGGAATTGCTGGTGTTGCACGAATGTTCGAGCGCCTAGACCTTACCTCAAAGAAACCTGGTGCCCCTACTCCAGTTTCTGGTCGTAGTGGGGGTACTTCAAACTTCTCCGTCAAGGGAAAAGCTGTGCTAGAGAATGTTATTGTTCAGTCGTGTAATAAAAGTAGTGGAAGAATTGCTGATGAAAGTTCAGAAGCACCCTCTCAGATCATCAGGCTGACTTGA

Protein Analysis

377

Amino Acids

41.16

Weight (kDa)

8.04

Isoelectric Point (pI)

60.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 31 - 77 1.7e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 32 - 84 2.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 35 - 77 2.8e-10 Ring finger domain
zf-RING_11 PF17123 37 - 64 7.4e-06 RING-like zinc finger
zf-RING_5 PF14634 37 - 77 9.6e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012488)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G14220
fragaria_vesca FvH4_4g14860
malus_domestica MD13G1185900.v1.1 MD16G1186700.v1.1
prunus_persica Prupe.1G153500_v2.0.a1
pyrus_communis pycom13g16040 pycom16g15650
rosa_chinensis RchiOBHm_Chr4g0417351 RchiOBHm_Chr4g0417381
rosa_laevigata RLG00000007915
rosa_multiflora Rmu_co8127690.1_g000001 Rmu_sc0004142.1_g000031
rosa_roxburghii Rroxscaffold_5G00361110
rosa_rugosa Rorug04G0147200
rosa_samantha Rh4AG210700 Rh4BG211200 Rh4CG221600 Rh4DG208600
rosa_wichuraiana Rw4G017800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 827, 971
AccB7I CCANNNNNTGG 1 cut(s) 989
AclWI GGATC 3 cut(s) 248, 261, 674
AcoI YGGCCR 1 cut(s) 706
AcuI CTGAAG 1 cut(s) 780
AfaI GTAC 3 cut(s) 285, 456, 1006
AfeI AGCGCT 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 500, 989
AgsI TTSAA 7 cut(s) 250, 371, 638, 688, 841, 896, 1011
AjnI CCWGG 2 cut(s) 595, 967
AluBI AGCT 4 cut(s) 162, 784, 850, 1034
AluI AGCT 4 cut(s) 162, 784, 850, 1034
AlwI GGATC 3 cut(s) 248, 261, 674
AlwNI CAGNNNCTG 2 cut(s) 117, 989
Ama87I CYCGRG 3 cut(s) 122, 764, 831
Aor51HI AGCGCT 1 cut(s) 299
AoxI GGCC 2 cut(s) 490, 706
ApaI GGGCCC 1 cut(s) 494
ApeKI GCWGC 8 cut(s) 162, 418, 428, 431, 660, 668, 791, 850
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AspLEI GCGC 2 cut(s) 300, 945
AspS9I GGNCC 3 cut(s) 490, 491, 725
AsuHPI GGTGA 4 cut(s) 574, 585, 600, 614
AsuNHI GCTAGC 1 cut(s) 260
AvaI CYCGRG 3 cut(s) 122, 764, 831
AvaII GGWCC 1 cut(s) 725
BaeGI GKGCMC 2 cut(s) 494, 976
BalI TGGCCA 1 cut(s) 708
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 827, 971
BanII GRGCYC 2 cut(s) 448, 494
BauI CACGAG 1 cut(s) 519
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 8 cut(s) 149, 415, 418, 430, 655, 672, 778, 837
BccI CCATC 5 cut(s) 59, 70, 655, 751, 824
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 2 cut(s) 597, 969
BfaI CTAG 5 cut(s) 261, 275, 438, 947, 1040
BfmI CTRYAG 4 cut(s) 109, 564, 658, 909
BfoI RGCGCY 2 cut(s) 301, 946
BisI GCNGC 8 cut(s) 163, 419, 429, 432, 661, 669, 792, 851
BlsI GCNGC 8 cut(s) 164, 420, 430, 433, 662, 670, 793, 852
Bme1390I CCNGG 2 cut(s) 597, 969
Bme18I GGWCC 1 cut(s) 725
BmeT110I CYCGRG 3 cut(s) 122, 764, 831
BmgT120I GGNCC 3 cut(s) 490, 491, 725
BmiI GGNNCC 4 cut(s) 255, 492, 829, 973
BmrFI CCNGG 2 cut(s) 597, 969
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 3 cut(s) 14, 430, 741
BmtI GCTAGC 1 cut(s) 264
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 108
BplI GAGNNNNNCTC 4 cut(s) 284, 316, 803, 835
BpmI CTGGAG 1 cut(s) 966
BpuEI CTTGAG 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 265, 832
Bsc4I CCNNNNNNNGG 2 cut(s) 500, 989
Bse1I ACTGG 2 cut(s) 492, 983
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 2 cut(s) 597, 969
BseDI CCNNGG 2 cut(s) 265, 832
BseGI GGATG 1 cut(s) 62
BseLI CCNNNNNNNGG 2 cut(s) 500, 989
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 5 cut(s) 345, 399, 738, 882, 1127
BseNI ACTGG 2 cut(s) 492, 983
BseRI GAGGAG 1 cut(s) 58
BseSI GKGCMC 2 cut(s) 494, 976
BseXI GCAGC 8 cut(s) 149, 415, 418, 430, 655, 672, 778, 837
BseYI CCCAGC 1 cut(s) 145
BshFI GGCC 2 cut(s) 492, 708
BshNI GGYRCC 2 cut(s) 827, 971
BsiHKCI CYCGRG 3 cut(s) 122, 764, 831
BsiSI CCGG 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 500, 989
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 2 cut(s) 492, 708
BsoBI CYCGRG 3 cut(s) 122, 764, 831
Bsp120I GGGCCC 1 cut(s) 490
Bsp1286I GDGCHC 3 cut(s) 448, 494, 976
Bsp1407I TGTACA 1 cut(s) 283
Bsp143I GATC 3 cut(s) 253, 679, 1116
BspANI GGCC 2 cut(s) 492, 708
BspCNI CTCAG 5 cut(s) 346, 398, 739, 881, 1126
BspLI GGNNCC 4 cut(s) 255, 492, 829, 973
BspMAI CTGCAG 3 cut(s) 113, 662, 913
BspOI GCTAGC 1 cut(s) 264
BspPI GGATC 3 cut(s) 248, 261, 674
BspT107I GGYRCC 2 cut(s) 827, 971
BsrDI GCAATG 1 cut(s) 324
BsrGI TGTACA 1 cut(s) 283
BsrI ACTGG 2 cut(s) 492, 983
BssECI CCNNGG 2 cut(s) 265, 832
BssMI GATC 3 cut(s) 253, 679, 1116
BssSI CACGAG 1 cut(s) 519
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 519
Bst2UI CCWGG 2 cut(s) 597, 969
Bst4CI ACNGT 1 cut(s) 188
Bst6I CTCTTC 1 cut(s) 28
BstAPI GCANNNNNTGC 2 cut(s) 117, 917
BstAUI TGTACA 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 262, 546, 710, 855
BstDEI CTNAG 5 cut(s) 354, 385, 747, 868, 1113
BstEII GGTNACC 1 cut(s) 591
BstF5I GGATG 1 cut(s) 62
BstH2I RGCGCY 2 cut(s) 301, 946
BstHHI GCGC 2 cut(s) 300, 945
BstKTI GATC 3 cut(s) 256, 682, 1119
BstMBI GATC 3 cut(s) 253, 679, 1116
BstMWI GCNNNNNNNGC 6 cut(s) 11, 117, 415, 797, 917, 926
BstNI CCWGG 2 cut(s) 597, 969
BstPI GGTNACC 1 cut(s) 591
BstSCI CCNGG 2 cut(s) 595, 967
BstSFI CTRYAG 4 cut(s) 109, 564, 658, 909
BstSLI GKGCMC 2 cut(s) 494, 976
BstV1I GCAGC 8 cut(s) 149, 415, 418, 430, 655, 672, 778, 837
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 253
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 1 cut(s) 253
BsuRI GGCC 2 cut(s) 492, 708
BtsCI GGATG 1 cut(s) 62
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 184, 663
Cac8I GCNNGC 4 cut(s) 262, 546, 710, 855
CaiI CAGNNNCTG 2 cut(s) 117, 989
CfoI GCGC 2 cut(s) 300, 945
Cfr13I GGNCC 3 cut(s) 490, 491, 725
CseI GACGC 1 cut(s) 423
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 3 cut(s) 284, 455, 1005
CviAII CATG 2 cut(s) 170, 374
CviQI GTAC 3 cut(s) 284, 455, 1005
DdeI CTNAG 5 cut(s) 354, 385, 747, 868, 1113
DpnI GATC 3 cut(s) 255, 681, 1118
DpnII GATC 3 cut(s) 253, 679, 1116
EaeI YGGCCR 1 cut(s) 706
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 448, 494
Eco47I GGWCC 1 cut(s) 725
Eco47III AGCGCT 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 780
Eco88I CYCGRG 3 cut(s) 122, 764, 831
Eco91I GGTNACC 1 cut(s) 591
EcoO109I RGGNCCY 1 cut(s) 491
EcoO65I GGTNACC 1 cut(s) 591
EcoRII CCWGG 2 cut(s) 595, 967
EcoT14I CCWWGG 1 cut(s) 265
EcoT38I GRGCYC 2 cut(s) 448, 494
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 2 cut(s) 173, 377
FatI CATG 2 cut(s) 169, 373
Fnu4HI GCNGC 8 cut(s) 163, 419, 429, 432, 661, 669, 792, 851
FokI GGATG 1 cut(s) 49
FriOI GRGCYC 2 cut(s) 448, 494
Fsp4HI GCNGC 8 cut(s) 163, 419, 429, 432, 661, 669, 792, 851
FspBI CTAG 5 cut(s) 261, 275, 438, 947, 1040
GlaI GCGC 2 cut(s) 299, 944
GluI GCNGC 8 cut(s) 163, 419, 429, 432, 661, 669, 792, 851
GsaI CCCAGC 1 cut(s) 149
GsuI CTGGAG 1 cut(s) 966
HaeII RGCGCY 2 cut(s) 301, 946
HaeIII GGCC 2 cut(s) 492, 708
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 423
HhaI GCGC 2 cut(s) 300, 945
Hin1II CATG 2 cut(s) 173, 377
Hin6I GCGC 2 cut(s) 298, 943
HinP1I GCGC 2 cut(s) 298, 943
HinfI GANTC 7 cut(s) 58, 104, 377, 392, 605, 767, 812
HpaII CCGG 1 cut(s) 257
HphI GGTGA 4 cut(s) 574, 585, 600, 614
Hpy188I TCNGA 8 cut(s) 37, 139, 355, 388, 397, 748, 1102, 1116
Hpy188III TCNNGA 7 cut(s) 55, 86, 247, 275, 483, 521, 764
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 5 cut(s) 27, 244, 456, 504, 835
HpyCH4III ACNGT 1 cut(s) 188
HpyCH4IV ACGT 2 cut(s) 571, 898
HpyCH4V TGCA 6 cut(s) 111, 165, 418, 660, 911, 929
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 117, 415, 797, 917, 926
HpyF3I CTNAG 5 cut(s) 354, 385, 747, 868, 1113
HpySE526I ACGT 2 cut(s) 571, 898
Hsp92II CATG 2 cut(s) 173, 377
HspAI GCGC 2 cut(s) 298, 943
Kzo9I GATC 3 cut(s) 253, 679, 1116
LmnI GCTCC 3 cut(s) 19, 140, 541
Lsp1109I GCAGC 8 cut(s) 149, 415, 418, 430, 655, 672, 778, 837
LweI GCATC 3 cut(s) 14, 430, 741
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 5 cut(s) 261, 275, 438, 947, 1040
MaeII ACGT 2 cut(s) 571, 898
MaeIII GTNAC 4 cut(s) 201, 237, 591, 860
MalI GATC 3 cut(s) 255, 681, 1118
MboI GATC 3 cut(s) 253, 679, 1116
MboII GAAGA 7 cut(s) 15, 18, 21, 24, 113, 312, 1092
MflI RGATCY 1 cut(s) 253
MhlI GDGCHC 3 cut(s) 448, 494, 976
MlsI TGGCCA 1 cut(s) 708
MluCI AATT 6 cut(s) 225, 346, 508, 757, 915, 1083
MluNI TGGCCA 1 cut(s) 708
MlyI GAGTC 5 cut(s) 52, 98, 386, 776, 821
Mox20I TGGCCA 1 cut(s) 708
MscI TGGCCA 1 cut(s) 708
MseI TTAA 1 cut(s) 507
Msp20I TGGCCA 1 cut(s) 708
MspA1I CMGCKG 1 cut(s) 162
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 2 cut(s) 597, 969
Mva1269I GAATGC 1 cut(s) 224
MvaI CCWGG 2 cut(s) 597, 969
MwoI GCNNNNNNNGC 6 cut(s) 11, 117, 415, 797, 917, 926
NdeII GATC 3 cut(s) 253, 679, 1116
NheI GCTAGC 1 cut(s) 260
NlaIII CATG 2 cut(s) 173, 377
NlaIV GGNNCC 4 cut(s) 255, 492, 829, 973
NmuCI GTSAC 2 cut(s) 201, 591
PaeR7I CTCGAG 1 cut(s) 122
PctI GAATGC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 377, 605
PflMI CCANNNNNTGG 1 cut(s) 989
PkrI GCNGC 8 cut(s) 164, 420, 430, 433, 662, 670, 793, 852
PleI GAGTC 5 cut(s) 52, 98, 386, 775, 820
PpsI GAGTC 5 cut(s) 52, 98, 386, 775, 820
Psp6I CCWGG 2 cut(s) 595, 967
PspEI GGTNACC 1 cut(s) 591
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 2 cut(s) 595, 967
PspN4I GGNNCC 4 cut(s) 255, 492, 829, 973
PspOMI GGGCCC 1 cut(s) 490
PspPI GGNCC 3 cut(s) 490, 491, 725
PspXI VCTCGAGB 1 cut(s) 122
PstI CTGCAG 3 cut(s) 113, 662, 913
PstNI CAGNNNCTG 2 cut(s) 117, 989
PsuI RGATCY 1 cut(s) 253
PvuII CAGCTG 1 cut(s) 162
RsaI GTAC 3 cut(s) 285, 456, 1006
RsaNI GTAC 3 cut(s) 284, 455, 1005
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 8 cut(s) 163, 419, 429, 432, 661, 669, 792, 851
Sau3AI GATC 3 cut(s) 253, 679, 1116
Sau96I GGNCC 3 cut(s) 490, 491, 725
SchI GAGTC 5 cut(s) 52, 98, 386, 776, 821
ScrFI CCNGG 2 cut(s) 597, 969
SduI GDGCHC 3 cut(s) 448, 494, 976
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 3 cut(s) 14, 430, 741
SfcI CTRYAG 4 cut(s) 109, 564, 658, 909
Sfr274I CTCGAG 1 cut(s) 122
SinI GGWCC 1 cut(s) 725
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 122, 301
SmoI CTYRAG 2 cut(s) 122, 301
Sse9I AATT 6 cut(s) 225, 346, 508, 757, 915, 1083
SspMI CTAG 5 cut(s) 261, 275, 438, 947, 1040
StyD4I CCNGG 2 cut(s) 595, 967
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 1 cut(s) 188
TaiI ACGT 2 cut(s) 574, 901
TaqI TCGA 2 cut(s) 123, 939
TasI AATT 6 cut(s) 225, 346, 508, 757, 915, 1083
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 2 cut(s) 377, 605
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 2 cut(s) 191, 670
TseFI GTSAC 2 cut(s) 201, 591
TseI GCWGC 8 cut(s) 162, 418, 428, 431, 660, 668, 791, 850
Tsp45I GTSAC 2 cut(s) 201, 591
TspDTI ATGAA 3 cut(s) 114, 332, 1107
TspGWI ACGGA 1 cut(s) 1009
TspRI CASTG 2 cut(s) 191, 670
Van91I CCANNNNNTGG 1 cut(s) 989
VpaK11BI GGWCC 1 cut(s) 725
XbaI TCTAGA 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 51
XhoI CTCGAG 1 cut(s) 122
XspI CTAG 5 cut(s) 261, 275, 438, 947, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.