AT4G20350
ERF Family

2OG-Fe(II) oxygenase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005744
Physical Location & Seq
Forward (+)
10988037 .. 10989947
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20350.10

Sequence Viewer

Length: 561 bp
ATGAAAAGGGTCTTGTTCCACAAGAGTGTAATGCCTCCCTGGCTGACAAAGATAACAGCGGAAATTCATGAAAGCTCGGGTCTGTTCCCTTCTGCTATAAATCATGTCCTCATCAATGAATACCATCCTGACCAAGGGATTATGCCGCATCAAGATGGACCCGCTTACTTTCCTGTTGTAGCTATCTTGTCTCTTGGCTCACCTGTTGTTATGGACTTCACTCCGCATTTGAGATTAAGATCAGGCGATGGTTACATTTCCAAAGACCAAAGTCCCTGTGCAGAGAGTTGTGCCCCTGAGAGAGACTCATTCTCTGTCTTGTTGATGCCTCAAAGCTTACTCATCTTCAAAGATGACGCATATTCAGATTTCCTCCACGGCATTAGCGATAGTCCAACACAATGTTACAACCAGGTCGTTAATGAAGCTGAAGCTTTGGCTTACTCTAACGAAGAAGATTCAAGAAAAGATGGTGACAAGATTTTTCACAGAGACCAAACTCGAGTTTCACTTACTTGCCGTCTAGTTCCCAAAGTCCGTAAAAATCTCTTCAGATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

186

Amino Acids

21.02

Weight (kDa)

6.04

Isoelectric Point (pI)

56.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 25 - 204 1.3e-10 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 59, 146, 162, 224
AcsI RAATTY 1 cut(s) 63
AcuI CTGAAG 2 cut(s) 450, 535
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 2 cut(s) 349, 462
AjnI CCWGG 2 cut(s) 38, 411
AleI CACNNNNGTG 1 cut(s) 24
AluBI AGCT 5 cut(s) 75, 182, 336, 428, 434
AluI AGCT 5 cut(s) 75, 182, 336, 428, 434
Alw26I GTCTC 3 cut(s) 195, 297, 486
Ama87I CYCGRG 2 cut(s) 76, 501
ApoI RAATTY 1 cut(s) 63
AspS9I GGNCC 1 cut(s) 158
AsuHPI GGTGA 2 cut(s) 192, 485
AvaI CYCGRG 2 cut(s) 76, 501
AvaII GGWCC 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 295
BccI CCATC 4 cut(s) 132, 149, 242, 464
BceAI ACGGC 2 cut(s) 394, 504
BciT130I CCWGG 2 cut(s) 40, 413
BcoDI GTCTC 3 cut(s) 195, 297, 486
BfaI CTAG 2 cut(s) 524, 559
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 40, 413
Bme18I GGWCC 1 cut(s) 158
BmeT110I CYCGRG 2 cut(s) 76, 501
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 2 cut(s) 40, 413
BmsI GCATC 2 cut(s) 157, 315
BoxI GACNNNNGTC 1 cut(s) 270
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BsaBI GATNNNNATC 1 cut(s) 238
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 38, 133, 376
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse8I GATNNNNATC 1 cut(s) 238
BseBI CCWGG 2 cut(s) 40, 413
BseDI CCNNGG 3 cut(s) 38, 133, 376
BseGI GGATG 1 cut(s) 124
BseJI GATNNNNATC 1 cut(s) 238
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 295
BsgI GTGCAG 1 cut(s) 300
BsiHKCI CYCGRG 2 cut(s) 76, 501
BslFI GGGAC 1 cut(s) 258
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 3 cut(s) 195, 297, 486
BsmFI GGGAC 1 cut(s) 258
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 2 cut(s) 76, 501
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 1 cut(s) 239
BspACI CCGC 4 cut(s) 59, 146, 162, 224
BspCNI CTCAG 1 cut(s) 289
BspHI TCATGA 1 cut(s) 67
BspLI GGNNCC 1 cut(s) 160
BspTNI GGTCTC 1 cut(s) 486
BssECI CCNNGG 3 cut(s) 38, 133, 376
BssMI GATC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 2 cut(s) 40, 413
Bst6I CTCTTC 1 cut(s) 554
BstDEI CTNAG 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 376
BstENI CCTNNNNNAGG 1 cut(s) 132
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 1 cut(s) 242
BstMAI GTCTC 3 cut(s) 195, 297, 486
BstMBI GATC 1 cut(s) 239
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstNI CCWGG 2 cut(s) 40, 413
BstPAI GACNNNNGTC 1 cut(s) 270
BstSCI CCNGG 2 cut(s) 38, 411
BstSLI GKGCMC 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 376
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 1 cut(s) 124
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 158
CseI GACGC 1 cut(s) 365
CsiI ACCWGGT 1 cut(s) 411
CviAII CATG 2 cut(s) 68, 104
CviJI RGCY 8 cut(s) 43, 75, 182, 198, 336, 428, 434, 440
CviKI_1 RGCY 8 cut(s) 43, 75, 182, 198, 336, 428, 434, 440
DdeI CTNAG 1 cut(s) 297
DpnI GATC 1 cut(s) 241
DpnII GATC 1 cut(s) 239
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 486
Eco47I GGWCC 1 cut(s) 158
Eco57I CTGAAG 2 cut(s) 450, 535
Eco88I CYCGRG 2 cut(s) 76, 501
EcoNI CCTNNNNNAGG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 38, 411
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 2 cut(s) 71, 107
FaiI YATR 6 cut(s) 69, 98, 105, 143, 212, 361
FaqI GGGAC 1 cut(s) 258
FatI CATG 2 cut(s) 67, 103
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 1 cut(s) 146
FokI GGATG 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 146
FspBI CTAG 2 cut(s) 524, 559
GluI GCNGC 1 cut(s) 146
HgaI GACGC 1 cut(s) 365
Hin1II CATG 2 cut(s) 71, 107
HindIII AAGCTT 2 cut(s) 334, 432
HinfI GANTC 3 cut(s) 305, 458, 555
HphI GGTGA 2 cut(s) 192, 485
Hpy188I TCNGA 2 cut(s) 367, 554
Hpy188III TCNNGA 4 cut(s) 68, 128, 152, 462
HpyAV CCTTC 1 cut(s) 99
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpyF3I CTNAG 1 cut(s) 297
Hsp92II CATG 2 cut(s) 71, 107
Kzo9I GATC 1 cut(s) 239
LweI GCATC 2 cut(s) 157, 315
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 2 cut(s) 524, 559
MaeIII GTNAC 3 cut(s) 251, 404, 473
MalI GATC 1 cut(s) 241
MboI GATC 1 cut(s) 239
MboII GAAGA 4 cut(s) 337, 464, 467, 541
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 1 cut(s) 63
MlyI GAGTC 1 cut(s) 299
MmeI TCCRAC 1 cut(s) 419
MnlI CCTC 4 cut(s) 45, 119, 339, 383
MseI TTAA 2 cut(s) 236, 420
MslI CAYNNNNRTG 2 cut(s) 24, 153
MspA1I CMGCKG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 40, 413
MvaI CCWGG 2 cut(s) 40, 413
MwoI GCNNNNNNNGC 1 cut(s) 40
NdeII GATC 1 cut(s) 239
NlaIII CATG 2 cut(s) 71, 107
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 1 cut(s) 67
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PfeI GAWTC 2 cut(s) 458, 555
PkrI GCNGC 1 cut(s) 147
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PshAI GACNNNNGTC 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 38, 411
PspGI CCWGG 2 cut(s) 38, 411
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 1 cut(s) 158
PspXI VCTCGAGB 1 cut(s) 501
RseI CAYNNNNRTG 2 cut(s) 24, 153
SaqAI TTAA 2 cut(s) 236, 420
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 1 cut(s) 239
Sau96I GGNCC 1 cut(s) 158
SchI GAGTC 1 cut(s) 299
ScrFI CCNGG 2 cut(s) 40, 413
SduI GDGCHC 1 cut(s) 295
SetI ASST 7 cut(s) 77, 184, 205, 338, 417, 430, 436
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 2 cut(s) 157, 315
Sfr274I CTCGAG 1 cut(s) 501
SinI GGWCC 1 cut(s) 158
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 2 cut(s) 24, 153
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 1 cut(s) 63
SsiI CCGC 4 cut(s) 59, 146, 162, 224
SspMI CTAG 2 cut(s) 524, 559
StyD4I CCNGG 2 cut(s) 38, 411
StyI CCWWGG 1 cut(s) 133
TaqI TCGA 1 cut(s) 502
TasI AATT 1 cut(s) 63
TauI GCSGC 1 cut(s) 148
TfiI GAWTC 2 cut(s) 458, 555
Tru1I TTAA 2 cut(s) 236, 420
Tru9I TTAA 2 cut(s) 236, 420
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 5 cut(s) 17, 56, 84, 132, 438
TspGWI ACGGA 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 158
XagI CCTNNNNNAGG 1 cut(s) 132
XapI RAATTY 1 cut(s) 63
XhoI CTCGAG 1 cut(s) 501
XspI CTAG 2 cut(s) 524, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.