Rh5DG576300

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005744
Physical Location & Seq
Forward (+)
88450774 .. 88458588
7815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG576300.1

Sequence Viewer

Length: 639 bp
ATGACGTCTTGTCAATGTCTTGTGTGGTGTTGTCTTAACAGAGAAATGGAAGGAAACCAGGCACTAAATAATTTCAAAGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCTAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGGACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATTGGGCGCAGGCACATGTACACATAATGCTATGTATTCAAATTCTGATGTGGGACTTTTCAAGAGTGATACAGATAAGTCGTTGGATGAACACTACCCATTTCCTGTCTTATTGATGCCGCGCAGTTTGCTTATATTCAAAGATAAAGCATACTCGGGTTGGTATACTTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

212

Amino Acids

23.95

Weight (kDa)

6.29

Isoelectric Point (pI)

47.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 67 - 153 9.3e-09 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 583
AciI CCGC 1 cut(s) 581
AclWI GGATC 1 cut(s) 91
AcsI RAATTY 2 cut(s) 436, 502
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 481
AfiI CCNNNNNNNGG 1 cut(s) 356
AflIII ACRYGT 1 cut(s) 476
AgsI TTSAA 5 cut(s) 76, 453, 501, 523, 601
AhdI GACNNNNNGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 57, 372
AluBI AGCT 1 cut(s) 386
AluI AGCT 1 cut(s) 386
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 1 cut(s) 91
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 1 cut(s) 380
ApoI RAATTY 2 cut(s) 436, 502
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 2 cut(s) 470, 585
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 414
AvaI CYCGRG 1 cut(s) 616
BarI GAAGNNNNNNTAC 2 cut(s) 123, 155
BccI CCATC 2 cut(s) 319, 371
BciT130I CCWGG 2 cut(s) 59, 374
BcoDI GTCTC 1 cut(s) 417
BisI GCNGC 1 cut(s) 581
BlsI GCNGC 1 cut(s) 582
Bme1390I CCNGG 2 cut(s) 59, 374
BmeRI GACNNNNNGTC 1 cut(s) 9
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 1 cut(s) 380
BmrFI CCNGG 2 cut(s) 59, 374
BmrI ACTGGG 1 cut(s) 220
BmsI GCATC 2 cut(s) 151, 567
BmuI ACTGGG 1 cut(s) 220
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 215, 395
BseBI CCWGG 2 cut(s) 59, 374
BseDI CCNNGG 1 cut(s) 355
BseGI GGATG 2 cut(s) 382, 553
BseLI CCNNNNNNNGG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 215, 395
Bsh1236I CGCG 1 cut(s) 583
BshFI GGCC 1 cut(s) 382
BsiHKCI CYCGRG 1 cut(s) 616
BslFI GGGAC 2 cut(s) 381, 528
BslI CCNNNNNNNGG 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 2 cut(s) 381, 528
BsnI GGCC 1 cut(s) 382
BsoBI CYCGRG 1 cut(s) 616
Bsp1407I TGTACA 1 cut(s) 479
Bsp143I GATC 2 cut(s) 83, 273
BspACI CCGC 1 cut(s) 581
BspANI GGCC 1 cut(s) 382
BspFNI CGCG 1 cut(s) 583
BspHI TCATGA 1 cut(s) 226
BspPI GGATC 1 cut(s) 91
BsrGI TGTACA 1 cut(s) 479
BsrI ACTGG 2 cut(s) 215, 395
BssECI CCNNGG 1 cut(s) 355
BssMI GATC 2 cut(s) 83, 273
BssNAI GTATAC 1 cut(s) 627
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 355
Bst1107I GTATAC 1 cut(s) 627
Bst2UI CCWGG 2 cut(s) 59, 374
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 479
BstC8I GCNNGC 2 cut(s) 384, 472
BstEII GGTNACC 1 cut(s) 420
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 2 cut(s) 382, 553
BstFNI CGCG 1 cut(s) 583
BstHHI GCGC 2 cut(s) 470, 585
BstKTI GATC 2 cut(s) 86, 276
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 2 cut(s) 83, 273
BstMWI GCNNNNNNNGC 1 cut(s) 589
BstNI CCWGG 2 cut(s) 59, 374
BstNSI RCATGY 2 cut(s) 282, 480
BstPI GGTNACC 1 cut(s) 420
BstSCI CCNGG 2 cut(s) 57, 372
BstUI CGCG 1 cut(s) 583
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BstZ17I GTATAC 1 cut(s) 627
BsuRI GGCC 1 cut(s) 382
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 2 cut(s) 382, 553
Cac8I GCNNGC 2 cut(s) 384, 472
CciI TCATGA 1 cut(s) 226
CfoI GCGC 2 cut(s) 470, 585
Cfr13I GGNCC 1 cut(s) 380
Csp6I GTAC 1 cut(s) 480
CviAII CATG 5 cut(s) 227, 261, 279, 326, 477
CviJI RGCY 4 cut(s) 202, 304, 382, 386
CviKI_1 RGCY 4 cut(s) 202, 304, 382, 386
CviQI GTAC 1 cut(s) 480
DpnI GATC 2 cut(s) 85, 275
DpnII GATC 2 cut(s) 83, 273
DraI TTTAAA 1 cut(s) 189
DriI GACNNNNNGTC 1 cut(s) 9
Eam1105I GACNNNNNGTC 1 cut(s) 9
Eco130I CCWWGG 1 cut(s) 355
Eco32I GATATC 1 cut(s) 243
Eco88I CYCGRG 1 cut(s) 616
Eco91I GGTNACC 1 cut(s) 420
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO65I GGTNACC 1 cut(s) 420
EcoRII CCWGG 2 cut(s) 57, 372
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 355
FaeI CATG 5 cut(s) 230, 264, 282, 329, 480
FaqI GGGAC 2 cut(s) 381, 528
FatI CATG 5 cut(s) 226, 260, 278, 325, 476
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 1 cut(s) 581
FokI GGATG 2 cut(s) 389, 560
Fsp4HI GCNGC 1 cut(s) 581
GlaI GCGC 2 cut(s) 469, 584
GluI GCNGC 1 cut(s) 581
HaeIII GGCC 1 cut(s) 382
HhaI GCGC 2 cut(s) 470, 585
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 230, 264, 282, 329, 480
Hin6I GCGC 2 cut(s) 468, 583
HinP1I GCGC 2 cut(s) 468, 583
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HinfI GANTC 1 cut(s) 296
HpaI GTTAAC 1 cut(s) 267
HphI GGTGA 1 cut(s) 414
Hpy166II GTNNAC 3 cut(s) 267, 482, 627
Hpy188I TCNGA 2 cut(s) 240, 508
Hpy188III TCNNGA 5 cut(s) 110, 227, 453, 523, 636
Hpy8I GTNNAC 3 cut(s) 267, 482, 627
HpyAV CCTTC 1 cut(s) 44
HpyCH4IV ACGT 1 cut(s) 5
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 1 cut(s) 589
HpySE526I ACGT 1 cut(s) 5
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 230, 264, 282, 329, 480
HspAI GCGC 2 cut(s) 468, 583
KspAI GTTAAC 1 cut(s) 267
Kzo9I GATC 2 cut(s) 83, 273
LweI GCATC 2 cut(s) 151, 567
MaeII ACGT 1 cut(s) 5
MaeIII GTNAC 1 cut(s) 420
MalI GATC 2 cut(s) 85, 275
MboI GATC 2 cut(s) 83, 273
MboII GAAGA 3 cut(s) 143, 305, 338
MflI RGATCY 1 cut(s) 83
MluCI AATT 5 cut(s) 70, 318, 405, 436, 502
MmeI TCCRAC 2 cut(s) 61, 525
MnlI CCTC 2 cut(s) 192, 268
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 5 cut(s) 36, 98, 188, 266, 321
MslI CAYNNNNRTG 1 cut(s) 375
MspR9I CCNGG 2 cut(s) 59, 374
MvaI CCWGG 2 cut(s) 59, 374
MvnI CGCG 1 cut(s) 583
MwoI GCNNNNNNNGC 1 cut(s) 589
NdeII GATC 2 cut(s) 83, 273
NlaIII CATG 5 cut(s) 230, 264, 282, 329, 480
NmuCI GTSAC 1 cut(s) 420
NspI RCATGY 2 cut(s) 282, 480
PagI TCATGA 1 cut(s) 226
PciI ACATGT 1 cut(s) 476
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 296
PkrI GCNGC 1 cut(s) 582
PscI ACATGT 1 cut(s) 476
Psp6I CCWGG 2 cut(s) 57, 372
PspEI GGTNACC 1 cut(s) 420
PspGI CCWGG 2 cut(s) 57, 372
PspPI GGNCC 1 cut(s) 380
PsuI RGATCY 1 cut(s) 83
RsaI GTAC 1 cut(s) 481
RsaNI GTAC 1 cut(s) 480
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 5 cut(s) 36, 98, 188, 266, 321
SatI GCNGC 1 cut(s) 581
Sau3AI GATC 2 cut(s) 83, 273
Sau96I GGNCC 1 cut(s) 380
ScrFI CCNGG 2 cut(s) 59, 374
SetI ASST 3 cut(s) 8, 388, 427
SfaNI GCATC 2 cut(s) 151, 567
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 5 cut(s) 70, 318, 405, 436, 502
SsiI CCGC 1 cut(s) 581
SspI AATATT 1 cut(s) 154
StyD4I CCNGG 2 cut(s) 57, 372
StyI CCWWGG 1 cut(s) 355
TaiI ACGT 1 cut(s) 8
TaqI TCGA 1 cut(s) 173
TasI AATT 5 cut(s) 70, 318, 405, 436, 502
TatI WGTACW 1 cut(s) 479
TauI GCSGC 1 cut(s) 583
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 5 cut(s) 36, 98, 188, 266, 321
Tru9I TTAA 5 cut(s) 36, 98, 188, 266, 321
TseFI GTSAC 1 cut(s) 420
Tsp45I GTSAC 1 cut(s) 420
TspDTI ATGAA 8 cut(s) 106, 144, 215, 243, 306, 354, 449, 564
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 2 cut(s) 436, 502
XceI RCATGY 2 cut(s) 282, 480
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 1 cut(s) 345
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.