Rw5G050440

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005744
Physical Location & Seq
Forward (+)
87052500 .. 87057924
5425 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G050440.1

Sequence Viewer

Length: 858 bp
ATGAGTGACCTAGTTCTATGTACCACTGTGGGTACTAACACATCTTCTTGTCTAGAAATGGAAGGAAACCAGGCAGTAAATAATTTCAAAGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCAAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGGACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATTGGGCGCAGGCACATGTACACATAATGCTATGTATTCAAATTCTGATGTGGGACTTTTCAAGAGTGATACAGATAAGTCGTTGGATGAACACCACCCATTTCCTGTCTTATTGATGCCACGCAGTTTGCTTATATTCAAAGATAAAGCATACTCAGATTACTTGCATGCTATAAACGACAGTGAGGTACACAGTTATGATAAGGTTGTAAACGAAGCACAAACTCGACCTTGGCAAGGCCTAGATCATCCATCTTCACAATTGGATGGAACTGATGAAACATTGAATACTGTGGACCTCAAGAGTATTCATAGAACTACCACTAGAGTTTCTTTAACATGTCGATTAGTTTTGAAGGTTCATAAAAATTTGTTCAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

285

Amino Acids

32.02

Weight (kDa)

6.16

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 71 - 273 9.5e-11 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 103
AcsI RAATTY 3 cut(s) 448, 514, 841
AfaI GTAC 4 cut(s) 22, 34, 493, 663
AfiI CCNNNNNNNGG 2 cut(s) 368, 710
AflIII ACRYGT 2 cut(s) 488, 812
AgsI TTSAA 8 cut(s) 88, 158, 465, 513, 535, 613, 760, 829
AjnI CCWGG 2 cut(s) 69, 384
AluBI AGCT 1 cut(s) 398
AluI AGCT 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 1 cut(s) 103
AoxI GGCC 2 cut(s) 392, 712
ApoI RAATTY 3 cut(s) 448, 514, 841
Asp700I GAANNNNTTC 1 cut(s) 357
AspLEI GCGC 1 cut(s) 482
AspS9I GGNCC 2 cut(s) 392, 769
AsuHPI GGTGA 1 cut(s) 426
AvaII GGWCC 1 cut(s) 769
BarI GAAGNNNNNNTAC 2 cut(s) 135, 167
BccI CCATC 4 cut(s) 331, 383, 733, 734
BciT130I CCWGG 2 cut(s) 71, 386
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 4 cut(s) 11, 53, 716, 798
Bme1390I CCNGG 2 cut(s) 71, 386
Bme18I GGWCC 1 cut(s) 769
BmgT120I GGNCC 2 cut(s) 392, 769
BmrFI CCNGG 2 cut(s) 71, 386
BmrI ACTGGG 1 cut(s) 232
BmsI GCATC 2 cut(s) 163, 579
BmuI ACTGGG 1 cut(s) 232
BpuEI CTTGAG 1 cut(s) 758
BsaJI CCNNGG 2 cut(s) 367, 704
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 710
Bse1I ACTGG 2 cut(s) 227, 407
BseBI CCWGG 2 cut(s) 71, 386
BseDI CCNNGG 2 cut(s) 367, 704
BseGI GGATG 4 cut(s) 394, 565, 721, 745
BseLI CCNNNNNNNGG 2 cut(s) 368, 710
BseMII CTCAG 1 cut(s) 642
BseNI ACTGG 2 cut(s) 227, 407
BshFI GGCC 2 cut(s) 394, 714
BslFI GGGAC 2 cut(s) 393, 540
BslI CCNNNNNNNGG 2 cut(s) 368, 710
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 2 cut(s) 393, 540
BsnI GGCC 2 cut(s) 394, 714
Bsp1407I TGTACA 1 cut(s) 491
Bsp143I GATC 3 cut(s) 95, 285, 718
BspANI GGCC 2 cut(s) 394, 714
BspCNI CTCAG 1 cut(s) 641
BspHI TCATGA 1 cut(s) 238
BspPI GGATC 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 491
BsrI ACTGG 2 cut(s) 227, 407
BssECI CCNNGG 2 cut(s) 367, 704
BssMI GATC 3 cut(s) 95, 285, 718
BssT1I CCWWGG 2 cut(s) 367, 704
Bst2UI CCWGG 2 cut(s) 71, 386
Bst4CI ACNGT 4 cut(s) 28, 656, 668, 766
BstAUI TGTACA 1 cut(s) 491
BstC8I GCNNGC 3 cut(s) 396, 484, 642
BstDEI CTNAG 1 cut(s) 628
BstEII GGTNACC 1 cut(s) 432
BstENI CCTNNNNNAGG 2 cut(s) 366, 708
BstF5I GGATG 4 cut(s) 394, 565, 721, 745
BstHHI GCGC 1 cut(s) 482
BstKTI GATC 3 cut(s) 98, 288, 721
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 3 cut(s) 95, 285, 718
BstNI CCWGG 2 cut(s) 71, 386
BstNSI RCATGY 4 cut(s) 294, 492, 644, 816
BstPI GGTNACC 1 cut(s) 432
BstSCI CCNGG 2 cut(s) 69, 384
BstX2I RGATCY 1 cut(s) 95
BstYI RGATCY 1 cut(s) 95
BsuRI GGCC 2 cut(s) 394, 714
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 4 cut(s) 394, 565, 721, 745
BtsIMutI CAGTG 2 cut(s) 24, 661
Cac8I GCNNGC 3 cut(s) 396, 484, 642
CciI TCATGA 1 cut(s) 238
CfoI GCGC 1 cut(s) 482
Cfr13I GGNCC 2 cut(s) 392, 769
Csp6I GTAC 4 cut(s) 21, 33, 492, 662
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 7 cut(s) 239, 273, 291, 338, 489, 641, 813
CviJI RGCY 5 cut(s) 214, 316, 394, 398, 714
CviKI_1 RGCY 5 cut(s) 214, 316, 394, 398, 714
CviQI GTAC 4 cut(s) 21, 33, 492, 662
DdeI CTNAG 1 cut(s) 628
DpnI GATC 3 cut(s) 97, 287, 720
DpnII GATC 3 cut(s) 95, 285, 718
DraI TTTAAA 1 cut(s) 201
Eco130I CCWWGG 2 cut(s) 367, 704
Eco147I AGGCCT 1 cut(s) 714
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 769
Eco91I GGTNACC 1 cut(s) 432
EcoNI CCTNNNNNAGG 2 cut(s) 366, 708
EcoO65I GGTNACC 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 69, 384
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 367, 704
ErhI CCWWGG 2 cut(s) 367, 704
FaeI CATG 7 cut(s) 242, 276, 294, 341, 492, 644, 816
FaqI GGGAC 2 cut(s) 393, 540
FatI CATG 7 cut(s) 238, 272, 290, 337, 488, 640, 812
FokI GGATG 4 cut(s) 401, 572, 708, 752
FspBI CTAG 4 cut(s) 11, 53, 716, 798
GlaI GCGC 1 cut(s) 481
HaeIII GGCC 2 cut(s) 394, 714
HhaI GCGC 1 cut(s) 482
Hin1II CATG 7 cut(s) 242, 276, 294, 341, 492, 644, 816
Hin6I GCGC 1 cut(s) 480
HinP1I GCGC 1 cut(s) 480
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HinfI GANTC 1 cut(s) 308
HpaI GTTAAC 1 cut(s) 279
HphI GGTGA 1 cut(s) 426
Hpy166II GTNNAC 5 cut(s) 279, 494, 664, 685, 769
Hpy188I TCNGA 3 cut(s) 252, 520, 631
Hpy188III TCNNGA 6 cut(s) 53, 122, 239, 465, 535, 775
Hpy8I GTNNAC 5 cut(s) 279, 494, 664, 685, 769
HpyAV CCTTC 2 cut(s) 56, 823
HpyCH4III ACNGT 4 cut(s) 28, 656, 668, 766
HpyCH4V TGCA 2 cut(s) 294, 640
HpyF3I CTNAG 1 cut(s) 628
Hsp92II CATG 7 cut(s) 242, 276, 294, 341, 492, 644, 816
HspAI GCGC 1 cut(s) 480
KspAI GTTAAC 1 cut(s) 279
Kzo9I GATC 3 cut(s) 95, 285, 718
LweI GCATC 2 cut(s) 163, 579
MaeI CTAG 4 cut(s) 11, 53, 716, 798
MaeIII GTNAC 2 cut(s) 5, 432
MalI GATC 3 cut(s) 97, 287, 720
MboI GATC 3 cut(s) 95, 285, 718
MboII GAAGA 5 cut(s) 36, 155, 317, 350, 720
MfeI CAATTG 1 cut(s) 734
MflI RGATCY 1 cut(s) 95
MluCI AATT 7 cut(s) 82, 330, 417, 448, 514, 734, 841
MmeI TCCRAC 2 cut(s) 73, 537
MnlI CCTC 4 cut(s) 204, 280, 652, 782
MroXI GAANNNNTTC 1 cut(s) 357
MseI TTAA 5 cut(s) 110, 200, 278, 333, 809
MslI CAYNNNNRTG 2 cut(s) 387, 669
MspR9I CCNGG 2 cut(s) 71, 386
MunI CAATTG 1 cut(s) 734
MvaI CCWGG 2 cut(s) 71, 386
NdeII GATC 3 cut(s) 95, 285, 718
NlaIII CATG 7 cut(s) 242, 276, 294, 341, 492, 644, 816
NmuCI GTSAC 2 cut(s) 5, 432
NspI RCATGY 4 cut(s) 294, 492, 644, 816
PaeI GCATGC 1 cut(s) 644
PagI TCATGA 1 cut(s) 238
PceI AGGCCT 1 cut(s) 714
PciI ACATGT 2 cut(s) 488, 812
PdmI GAANNNNTTC 1 cut(s) 357
PfeI GAWTC 1 cut(s) 308
PscI ACATGT 2 cut(s) 488, 812
Psp6I CCWGG 2 cut(s) 69, 384
PspEI GGTNACC 1 cut(s) 432
PspGI CCWGG 2 cut(s) 69, 384
PspPI GGNCC 2 cut(s) 392, 769
PsuI RGATCY 1 cut(s) 95
RsaI GTAC 4 cut(s) 22, 34, 493, 663
RsaNI GTAC 4 cut(s) 21, 33, 492, 662
RseI CAYNNNNRTG 2 cut(s) 387, 669
SaqAI TTAA 5 cut(s) 110, 200, 278, 333, 809
Sau3AI GATC 3 cut(s) 95, 285, 718
Sau96I GGNCC 2 cut(s) 392, 769
ScrFI CCNGG 2 cut(s) 71, 386
SetI ASST 9 cut(s) 12, 400, 439, 663, 681, 706, 774, 834, 854
SfaNI GCATC 2 cut(s) 163, 579
SinI GGWCC 1 cut(s) 769
SmiMI CAYNNNNRTG 2 cut(s) 387, 669
SmlI CTYRAG 1 cut(s) 773
SmoI CTYRAG 1 cut(s) 773
SphI GCATGC 1 cut(s) 644
Sse9I AATT 7 cut(s) 82, 330, 417, 448, 514, 734, 841
SseBI AGGCCT 1 cut(s) 714
SspI AATATT 1 cut(s) 166
SspMI CTAG 4 cut(s) 11, 53, 716, 798
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 69, 384
StyI CCWWGG 2 cut(s) 367, 704
TaaI ACNGT 4 cut(s) 28, 656, 668, 766
TaqI TCGA 3 cut(s) 185, 700, 817
TasI AATT 7 cut(s) 82, 330, 417, 448, 514, 734, 841
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 5 cut(s) 110, 200, 278, 333, 809
Tru9I TTAA 5 cut(s) 110, 200, 278, 333, 809
TscAI CASTG 2 cut(s) 31, 661
TseFI GTSAC 2 cut(s) 5, 432
Tsp45I GTSAC 2 cut(s) 5, 432
TspRI CASTG 2 cut(s) 31, 661
VpaK11BI GGWCC 1 cut(s) 769
XagI CCTNNNNNAGG 2 cut(s) 366, 708
XapI RAATTY 3 cut(s) 448, 514, 841
XbaI TCTAGA 1 cut(s) 52
XceI RCATGY 4 cut(s) 294, 492, 644, 816
XmnI GAANNNNTTC 1 cut(s) 357
XspI CTAG 4 cut(s) 11, 53, 716, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.