RchiOBHm_Chr5g0084171

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005744
Physical Location & Seq
Forward (+)
89849978 .. 89855525
5548 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35825

Sequence Viewer

Length: 801 bp
ATGGAAGGAAACCAGGCACTAAATAATTTCAAAGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCTAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGGACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATTGGGCGCAGGCACATGTACACATAATGCTATGTATTCAAATTCTGATGTGGGACTTTTCAAGAGTGATACAGATAAGTCGTTGGATGAACACCACCCATTTCCTGTCTTATTGATGCCGTGCAGTTTGCTTATATTCAAAGATAAAGCATACTCAGATTACTTGCATGCTATAAACGACAGTGAGGTGCACAGTTATGATAAGGTTGTAAACGAAGCACAAGCTCGACCTTGGCAAGGCCTAGATCATCCATCTTCACAGTTGGATGGCACTGTTGAAACATTGAATACTGTGGACCTCAAGAGTATTCATAGAACTACCACTAGAGTTTCTTTAACATGTCGATTAGTTTTGAAAGTTCATAAAAATTTGTTCAGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

266

Amino Acids

29.96

Weight (kDa)

6.45

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 52 - 254 1.2e-10 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 3 cut(s) 391, 457, 784
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 2 cut(s) 311, 653
AflIII ACRYGT 2 cut(s) 431, 755
AgsI TTSAA 8 cut(s) 31, 408, 456, 478, 556, 695, 703, 772
AjnI CCWGG 2 cut(s) 12, 327
AluBI AGCT 2 cut(s) 341, 641
AluI AGCT 2 cut(s) 341, 641
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 1 cut(s) 372
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 1 cut(s) 46
AoxI GGCC 2 cut(s) 335, 655
ApaLI GTGCAC 1 cut(s) 605
ApoI RAATTY 3 cut(s) 391, 457, 784
Asp700I GAANNNNTTC 1 cut(s) 300
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 335, 712
AsuHPI GGTGA 1 cut(s) 369
AvaII GGWCC 1 cut(s) 712
BaeGI GKGCMC 1 cut(s) 609
BarI GAAGNNNNNNTAC 2 cut(s) 78, 110
Bbv12I GWGCWC 1 cut(s) 609
BccI CCATC 4 cut(s) 274, 326, 676, 677
BceAI ACGGC 1 cut(s) 520
BciT130I CCWGG 2 cut(s) 14, 329
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 2 cut(s) 659, 741
Bme1390I CCNGG 2 cut(s) 14, 329
Bme18I GGWCC 1 cut(s) 712
BmgT120I GGNCC 2 cut(s) 335, 712
BmrFI CCNGG 2 cut(s) 14, 329
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 2 cut(s) 106, 522
BmuI ACTGGG 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 701
BsaJI CCNNGG 2 cut(s) 310, 647
Bsc4I CCNNNNNNNGG 2 cut(s) 311, 653
Bse1I ACTGG 2 cut(s) 170, 350
BseBI CCWGG 2 cut(s) 14, 329
BseDI CCNNGG 2 cut(s) 310, 647
BseGI GGATG 4 cut(s) 337, 508, 664, 688
BseLI CCNNNNNNNGG 2 cut(s) 311, 653
BseMII CTCAG 1 cut(s) 585
BseNI ACTGG 2 cut(s) 170, 350
BseSI GKGCMC 1 cut(s) 609
BsgI GTGCAG 1 cut(s) 559
BshFI GGCC 2 cut(s) 337, 657
BsiHKAI GWGCWC 1 cut(s) 609
BslFI GGGAC 2 cut(s) 336, 483
BslI CCNNNNNNNGG 2 cut(s) 311, 653
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 336, 483
BsnI GGCC 2 cut(s) 337, 657
Bsp1286I GDGCHC 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 434
Bsp143I GATC 3 cut(s) 38, 228, 661
BspANI GGCC 2 cut(s) 337, 657
BspCNI CTCAG 1 cut(s) 584
BspHI TCATGA 1 cut(s) 181
BspPI GGATC 1 cut(s) 46
BsrGI TGTACA 1 cut(s) 434
BsrI ACTGG 2 cut(s) 170, 350
BssECI CCNNGG 2 cut(s) 310, 647
BssMI GATC 3 cut(s) 38, 228, 661
BssT1I CCWWGG 2 cut(s) 310, 647
Bst2UI CCWGG 2 cut(s) 14, 329
Bst4CI ACNGT 5 cut(s) 599, 611, 678, 691, 709
BstAUI TGTACA 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 339, 427, 585
BstDEI CTNAG 1 cut(s) 571
BstEII GGTNACC 1 cut(s) 375
BstENI CCTNNNNNAGG 2 cut(s) 309, 651
BstF5I GGATG 4 cut(s) 337, 508, 664, 688
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 3 cut(s) 41, 231, 664
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 3 cut(s) 38, 228, 661
BstNI CCWGG 2 cut(s) 14, 329
BstNSI RCATGY 4 cut(s) 237, 435, 587, 759
BstPI GGTNACC 1 cut(s) 375
BstSCI CCNGG 2 cut(s) 12, 327
BstSLI GKGCMC 1 cut(s) 609
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuRI GGCC 2 cut(s) 337, 657
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 4 cut(s) 337, 508, 664, 688
BtsIMutI CAGTG 2 cut(s) 604, 687
Cac8I GCNNGC 3 cut(s) 339, 427, 585
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 425
Cfr13I GGNCC 2 cut(s) 335, 712
Csp6I GTAC 1 cut(s) 435
CviAII CATG 7 cut(s) 182, 216, 234, 281, 432, 584, 756
CviJI RGCY 6 cut(s) 157, 259, 337, 341, 641, 657
CviKI_1 RGCY 6 cut(s) 157, 259, 337, 341, 641, 657
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 1 cut(s) 571
DpnI GATC 3 cut(s) 40, 230, 663
DpnII GATC 3 cut(s) 38, 228, 661
DraI TTTAAA 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 310, 647
Eco147I AGGCCT 1 cut(s) 657
Eco32I GATATC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 712
Eco91I GGTNACC 1 cut(s) 375
EcoNI CCTNNNNNAGG 2 cut(s) 309, 651
EcoO65I GGTNACC 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 12, 327
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 310, 647
ErhI CCWWGG 2 cut(s) 310, 647
FaeI CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
FaqI GGGAC 2 cut(s) 336, 483
FatI CATG 7 cut(s) 181, 215, 233, 280, 431, 583, 755
FokI GGATG 4 cut(s) 344, 515, 651, 695
FspBI CTAG 2 cut(s) 659, 741
GlaI GCGC 1 cut(s) 424
HaeIII GGCC 2 cut(s) 337, 657
HhaI GCGC 1 cut(s) 425
Hin1II CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 1 cut(s) 251
HpaI GTTAAC 1 cut(s) 222
HphI GGTGA 1 cut(s) 369
Hpy166II GTNNAC 5 cut(s) 222, 437, 607, 628, 712
Hpy188I TCNGA 3 cut(s) 195, 463, 574
Hpy188III TCNNGA 5 cut(s) 65, 182, 408, 478, 718
Hpy8I GTNNAC 5 cut(s) 222, 437, 607, 628, 712
HpyCH4III ACNGT 5 cut(s) 599, 611, 678, 691, 709
HpyCH4V TGCA 4 cut(s) 237, 540, 583, 607
HpyF3I CTNAG 1 cut(s) 571
Hsp92II CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
HspAI GCGC 1 cut(s) 423
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 38, 228, 661
LweI GCATC 2 cut(s) 106, 522
MaeI CTAG 2 cut(s) 659, 741
MaeIII GTNAC 1 cut(s) 375
MalI GATC 3 cut(s) 40, 230, 663
MboI GATC 3 cut(s) 38, 228, 661
MboII GAAGA 4 cut(s) 98, 260, 293, 663
MflI RGATCY 1 cut(s) 38
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 6 cut(s) 25, 273, 360, 391, 457, 784
MmeI TCCRAC 3 cut(s) 16, 480, 660
MnlI CCTC 4 cut(s) 147, 223, 595, 725
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 5 cut(s) 53, 143, 221, 276, 752
MslI CAYNNNNRTG 2 cut(s) 330, 612
MspR9I CCNGG 2 cut(s) 14, 329
MvaI CCWGG 2 cut(s) 14, 329
NdeII GATC 3 cut(s) 38, 228, 661
NlaIII CATG 7 cut(s) 185, 219, 237, 284, 435, 587, 759
NmuCI GTSAC 1 cut(s) 375
NspI RCATGY 4 cut(s) 237, 435, 587, 759
PaeI GCATGC 1 cut(s) 587
PagI TCATGA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 657
PciI ACATGT 2 cut(s) 431, 755
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 1 cut(s) 251
PscI ACATGT 2 cut(s) 431, 755
Psp6I CCWGG 2 cut(s) 12, 327
PspEI GGTNACC 1 cut(s) 375
PspGI CCWGG 2 cut(s) 12, 327
PspPI GGNCC 2 cut(s) 335, 712
PsuI RGATCY 1 cut(s) 38
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 2 cut(s) 330, 612
SaqAI TTAA 5 cut(s) 53, 143, 221, 276, 752
Sau3AI GATC 3 cut(s) 38, 228, 661
Sau96I GGNCC 2 cut(s) 335, 712
ScrFI CCNGG 2 cut(s) 14, 329
SduI GDGCHC 1 cut(s) 609
SetI ASST 8 cut(s) 343, 382, 606, 624, 643, 649, 717, 797
SfaNI GCATC 2 cut(s) 106, 522
SinI GGWCC 1 cut(s) 712
SmiMI CAYNNNNRTG 2 cut(s) 330, 612
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
SphI GCATGC 1 cut(s) 587
Sse9I AATT 6 cut(s) 25, 273, 360, 391, 457, 784
SseBI AGGCCT 1 cut(s) 657
SspI AATATT 1 cut(s) 109
SspMI CTAG 2 cut(s) 659, 741
StuI AGGCCT 1 cut(s) 657
StyD4I CCNGG 2 cut(s) 12, 327
StyI CCWWGG 2 cut(s) 310, 647
TaaI ACNGT 5 cut(s) 599, 611, 678, 691, 709
TaqI TCGA 3 cut(s) 128, 643, 760
TasI AATT 6 cut(s) 25, 273, 360, 391, 457, 784
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 5 cut(s) 53, 143, 221, 276, 752
Tru9I TTAA 5 cut(s) 53, 143, 221, 276, 752
TscAI CASTG 2 cut(s) 604, 694
TseFI GTSAC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 375
TspRI CASTG 2 cut(s) 604, 694
VneI GTGCAC 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 712
XagI CCTNNNNNAGG 2 cut(s) 309, 651
XapI RAATTY 3 cut(s) 391, 457, 784
XceI RCATGY 4 cut(s) 237, 435, 587, 759
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 2 cut(s) 659, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.