RLG00000037089

Alpha-ketoglutarate-dependent dioxygenase alkB homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005744
Physical Location & Seq
Forward (+)
88292835 .. 88300800
7966 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000037089

Sequence Viewer

Length: 969 bp
ATGCTGCCACAGTTCAGATATCGGAAATGGAAGGAAGAATTTGAGTTAGAAATGGAAGGAAACCAGGCACTAAATAATTTCAAAGTTGGATCTCTGCCCACTTTAATATACATTCCTGACTTCATTACAGAAAATGAAGAAACTATACTTCTAAAAAATATTTATGATGCCCCTGTATCGAAGTGGAAATCTTTAAAGAATAGGAGGCTACAAAACTGGGGTGGTGTAGTTCATGAAAAGGGTCTGATATCACAGGATTTGCCCTCATGGTTAACAAAGATCACATGCAGGATATATGAAGAATCTGGGCTATTCCCATCGCCAATTAACCATGTCCTTATCAATGAATATCTTCCTAACCAAGGCATAATGGCACACCAGGATGGGCCAGCTTACTATCCAGTTGTAGCAATTCTGTCTCTTGGGTCACCTGTTGTTATGAATTTCACTCCCCATTCAAGATTGACATGGGGTGCAGGCACATGTACACATAATGCTATGTATTCAAATTCTGATGGGGGACTTTTCAAGAGTGATACAGATAAGTCGTCGGATGAACACCACCCATTTCCTGTCTTATTGATGCCGCGCAGTTTGCTTATATTCAAAGATAAAGCATACTCGGATTACTTGCATGATATAAACGACAGTGAGGTACACAGTTATGATAAGGCTGTAAACGAAGCACAAGCTCGACCTTGGCAAGGTCTAGATCATCCATCTTCACAATTGGATGGAACTGTTGAAACATCGAATAGTGTGGACCTCAAGATCGCAACTCGCAACAGGCAACAGGGAGGCGAACAAAAGAAGGAAGGCAGAAAGCAGGAAGGAGACACAGTAGGTTGCCTTATCTTGCACCTATTCAGAAGATATTTGAATCCCGGTGCCTGTACTGAAAAAAAGAAGAAGGAAATTGTCCTTCTAATTTCTGTTTCGTCCAAGTGCTGTGAAGGGGCTCGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy_2

Protein Analysis

323

Amino Acids

36.53

Weight (kDa)

6.6

Isoelectric Point (pI)

43.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy_2 PF13532 69 - 216 7.1e-10 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 887
AccII CGCG 1 cut(s) 589
AciI CCGC 1 cut(s) 587
AclWI GGATC 1 cut(s) 97
AcsI RAATTY 3 cut(s) 38, 442, 508
AfaI GTAC 3 cut(s) 487, 657, 895
AfiI CCNNNNNNNGG 2 cut(s) 362, 704
AflIII ACRYGT 1 cut(s) 482
AgsI TTSAA 7 cut(s) 82, 459, 507, 529, 607, 746, 880
AjnI CCWGG 2 cut(s) 63, 378
AluBI AGCT 2 cut(s) 392, 692
AluI AGCT 2 cut(s) 392, 692
Alw26I GTCTC 2 cut(s) 423, 828
AlwI GGATC 1 cut(s) 97
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 3 cut(s) 38, 442, 508
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 2 cut(s) 386, 763
AsuC2I CCSGG 1 cut(s) 885
AsuHPI GGTGA 1 cut(s) 420
AvaII GGWCC 1 cut(s) 763
BanI GGYRCC 1 cut(s) 887
BanII GRGCYC 1 cut(s) 961
BarI GAAGNNNNNNTAC 2 cut(s) 129, 161
BccI CCATC 5 cut(s) 325, 377, 509, 727, 728
BciT130I CCWGG 2 cut(s) 65, 380
BcnI CCSGG 1 cut(s) 885
BcoDI GTCTC 2 cut(s) 423, 828
BfaI CTAG 1 cut(s) 710
BisI GCNGC 2 cut(s) 5, 587
BlsI GCNGC 2 cut(s) 6, 588
Bme1390I CCNGG 3 cut(s) 65, 380, 885
Bme18I GGWCC 1 cut(s) 763
BmgT120I GGNCC 2 cut(s) 386, 763
BmiI GGNNCC 1 cut(s) 889
BmrFI CCNGG 3 cut(s) 65, 380, 885
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 2 cut(s) 157, 573
BmuI ACTGGG 1 cut(s) 226
BpuEI CTTGAG 1 cut(s) 752
BpuMI CCSGG 1 cut(s) 885
BsaJI CCNNGG 2 cut(s) 361, 698
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 704
Bse1I ACTGG 2 cut(s) 221, 401
BseBI CCWGG 2 cut(s) 65, 380
BseDI CCNNGG 2 cut(s) 361, 698
BseGI GGATG 4 cut(s) 388, 559, 715, 739
BseLI CCNNNNNNNGG 2 cut(s) 362, 704
BseNI ACTGG 2 cut(s) 221, 401
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 589
BshFI GGCC 1 cut(s) 388
BshNI GGYRCC 1 cut(s) 887
BsiSI CCGG 1 cut(s) 885
BslFI GGGAC 1 cut(s) 534
BslI CCNNNNNNNGG 2 cut(s) 362, 704
BsmAI GTCTC 2 cut(s) 423, 828
BsmFI GGGAC 1 cut(s) 534
BsnI GGCC 1 cut(s) 388
Bsp1286I GDGCHC 1 cut(s) 961
Bsp1407I TGTACA 1 cut(s) 485
Bsp143I GATC 4 cut(s) 89, 279, 712, 771
BspACI CCGC 1 cut(s) 587
BspANI GGCC 1 cut(s) 388
BspFNI CGCG 1 cut(s) 589
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 889
BspPI GGATC 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 887
BsrGI TGTACA 1 cut(s) 485
BsrI ACTGG 2 cut(s) 221, 401
BssECI CCNNGG 2 cut(s) 361, 698
BssMI GATC 4 cut(s) 89, 279, 712, 771
BssT1I CCWWGG 2 cut(s) 361, 698
Bst2UI CCWGG 2 cut(s) 65, 380
Bst4CI ACNGT 5 cut(s) 12, 650, 662, 742, 841
BstAUI TGTACA 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 390, 478
BstEII GGTNACC 1 cut(s) 426
BstENI CCTNNNNNAGG 2 cut(s) 360, 702
BstF5I GGATG 4 cut(s) 388, 559, 715, 739
BstFNI CGCG 1 cut(s) 589
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 4 cut(s) 92, 282, 715, 774
BstMAI GTCTC 2 cut(s) 423, 828
BstMBI GATC 4 cut(s) 89, 279, 712, 771
BstMWI GCNNNNNNNGC 1 cut(s) 595
BstNI CCWGG 2 cut(s) 65, 380
BstNSI RCATGY 2 cut(s) 288, 486
BstPI GGTNACC 1 cut(s) 426
BstSCI CCNGG 3 cut(s) 63, 378, 883
BstUI CGCG 1 cut(s) 589
BstX2I RGATCY 1 cut(s) 89
BstYI RGATCY 1 cut(s) 89
BsuRI GGCC 1 cut(s) 388
BtgZI GCGATG 1 cut(s) 303
BtsCI GGATG 4 cut(s) 388, 559, 715, 739
BtsIMutI CAGTG 1 cut(s) 655
Cac8I GCNNGC 2 cut(s) 390, 478
CciI TCATGA 1 cut(s) 232
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 2 cut(s) 386, 763
Csp6I GTAC 3 cut(s) 486, 656, 894
CviAII CATG 7 cut(s) 233, 267, 285, 332, 468, 483, 635
CviJI RGCY 7 cut(s) 208, 310, 388, 392, 674, 692, 959
CviKI_1 RGCY 7 cut(s) 208, 310, 388, 392, 674, 692, 959
CviQI GTAC 3 cut(s) 486, 656, 894
DpnI GATC 4 cut(s) 91, 281, 714, 773
DpnII GATC 4 cut(s) 89, 279, 712, 771
DraI TTTAAA 1 cut(s) 195
Eco130I CCWWGG 2 cut(s) 361, 698
Eco24I GRGCYC 1 cut(s) 961
Eco32I GATATC 2 cut(s) 20, 249
Eco47I GGWCC 1 cut(s) 763
Eco91I GGTNACC 1 cut(s) 426
EcoNI CCTNNNNNAGG 2 cut(s) 360, 702
EcoO65I GGTNACC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 63, 378
EcoRV GATATC 2 cut(s) 20, 249
EcoT14I CCWWGG 2 cut(s) 361, 698
EcoT38I GRGCYC 1 cut(s) 961
ErhI CCWWGG 2 cut(s) 361, 698
FaeI CATG 7 cut(s) 236, 270, 288, 335, 471, 486, 638
FaqI GGGAC 1 cut(s) 534
FatI CATG 7 cut(s) 232, 266, 284, 331, 467, 482, 634
Fnu4HI GCNGC 2 cut(s) 5, 587
FokI GGATG 4 cut(s) 395, 566, 702, 746
FriOI GRGCYC 1 cut(s) 961
Fsp4HI GCNGC 2 cut(s) 5, 587
FspBI CTAG 1 cut(s) 710
GlaI GCGC 1 cut(s) 590
GluI GCNGC 2 cut(s) 5, 587
HaeIII GGCC 1 cut(s) 388
HapII CCGG 1 cut(s) 885
HhaI GCGC 1 cut(s) 591
Hin1II CATG 7 cut(s) 236, 270, 288, 335, 471, 486, 638
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 2 cut(s) 302, 880
HpaI GTTAAC 1 cut(s) 273
HpaII CCGG 1 cut(s) 885
HphI GGTGA 1 cut(s) 420
Hpy166II GTNNAC 5 cut(s) 273, 488, 658, 679, 763
Hpy188I TCNGA 7 cut(s) 17, 24, 246, 514, 553, 625, 869
Hpy188III TCNNGA 6 cut(s) 116, 233, 459, 529, 710, 769
Hpy8I GTNNAC 5 cut(s) 273, 488, 658, 679, 763
Hpy99I CGWCG 1 cut(s) 553
HpyAV CCTTC 8 cut(s) 25, 50, 805, 809, 824, 904, 932, 947
HpyCH4III ACNGT 5 cut(s) 12, 650, 662, 742, 841
HpyCH4V TGCA 4 cut(s) 288, 476, 634, 859
HpyF10VI GCNNNNNNNGC 1 cut(s) 595
Hsp92II CATG 7 cut(s) 236, 270, 288, 335, 471, 486, 638
HspAI GCGC 1 cut(s) 589
KspAI GTTAAC 1 cut(s) 273
Kzo9I GATC 4 cut(s) 89, 279, 712, 771
LweI GCATC 2 cut(s) 157, 573
MaeI CTAG 1 cut(s) 710
MaeIII GTNAC 1 cut(s) 426
MalI GATC 4 cut(s) 91, 281, 714, 773
MboI GATC 4 cut(s) 89, 279, 712, 771
MboII GAAGA 7 cut(s) 47, 149, 311, 344, 714, 882, 919
MfeI CAATTG 1 cut(s) 728
MflI RGATCY 1 cut(s) 89
MhlI GDGCHC 1 cut(s) 961
MluCI AATT 9 cut(s) 38, 76, 324, 411, 442, 508, 728, 915, 927
MmeI TCCRAC 2 cut(s) 67, 531
MnlI CCTC 5 cut(s) 198, 274, 646, 776, 791
MroXI GAANNNNTTC 1 cut(s) 351
MseI TTAA 4 cut(s) 104, 194, 272, 327
MslI CAYNNNNRTG 2 cut(s) 381, 663
MspI CCGG 1 cut(s) 885
MspR9I CCNGG 3 cut(s) 65, 380, 885
MunI CAATTG 1 cut(s) 728
MvaI CCWGG 2 cut(s) 65, 380
MvnI CGCG 1 cut(s) 589
MwoI GCNNNNNNNGC 1 cut(s) 595
NciI CCSGG 1 cut(s) 885
NdeII GATC 4 cut(s) 89, 279, 712, 771
NlaIII CATG 7 cut(s) 236, 270, 288, 335, 471, 486, 638
NlaIV GGNNCC 1 cut(s) 889
NmuCI GTSAC 1 cut(s) 426
NspI RCATGY 2 cut(s) 288, 486
PagI TCATGA 1 cut(s) 232
PciI ACATGT 1 cut(s) 482
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 2 cut(s) 302, 880
PkrI GCNGC 2 cut(s) 6, 588
PscI ACATGT 1 cut(s) 482
Psp6I CCWGG 2 cut(s) 63, 378
PspEI GGTNACC 1 cut(s) 426
PspGI CCWGG 2 cut(s) 63, 378
PspN4I GGNNCC 1 cut(s) 889
PspPI GGNCC 2 cut(s) 386, 763
PsuI RGATCY 1 cut(s) 89
RsaI GTAC 3 cut(s) 487, 657, 895
RsaNI GTAC 3 cut(s) 486, 656, 894
RseI CAYNNNNRTG 2 cut(s) 381, 663
SaqAI TTAA 4 cut(s) 104, 194, 272, 327
SatI GCNGC 2 cut(s) 5, 587
Sau3AI GATC 4 cut(s) 89, 279, 712, 771
Sau96I GGNCC 2 cut(s) 386, 763
ScrFI CCNGG 3 cut(s) 65, 380, 885
SduI GDGCHC 1 cut(s) 961
SetI ASST 9 cut(s) 394, 433, 657, 694, 700, 709, 768, 847, 864
SfaNI GCATC 2 cut(s) 157, 573
SinI GGWCC 1 cut(s) 763
SmiMI CAYNNNNRTG 2 cut(s) 381, 663
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
Sse9I AATT 9 cut(s) 38, 76, 324, 411, 442, 508, 728, 915, 927
SsiI CCGC 1 cut(s) 587
SspI AATATT 1 cut(s) 160
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 3 cut(s) 63, 378, 883
StyI CCWWGG 2 cut(s) 361, 698
TaaI ACNGT 5 cut(s) 12, 650, 662, 742, 841
TaqI TCGA 3 cut(s) 179, 694, 752
TasI AATT 9 cut(s) 38, 76, 324, 411, 442, 508, 728, 915, 927
TatI WGTACW 2 cut(s) 485, 893
TauI GCSGC 1 cut(s) 589
TfiI GAWTC 2 cut(s) 302, 880
Tru1I TTAA 4 cut(s) 104, 194, 272, 327
Tru9I TTAA 4 cut(s) 104, 194, 272, 327
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 1 cut(s) 426
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 426
TspDTI ATGAA 8 cut(s) 112, 150, 221, 249, 312, 360, 455, 570
TspRI CASTG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 763
XagI CCTNNNNNAGG 2 cut(s) 360, 702
XapI RAATTY 3 cut(s) 38, 442, 508
XbaI TCTAGA 1 cut(s) 709
XceI RCATGY 2 cut(s) 288, 486
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.